Dear microscopists, as you may already know, FILM is about to expand to Hammersmith Campus, setting up two brand-new microscopes in the new L-Block building. With generous funding from a BHF fund to NHLI, two top-class microscopes have been purchased, a Zeiss LSM-780 confocal with GaAsP metadetector and an AxioObserver with LED illumination for fast multi-channel acquisition, a Hamamatsu Flash 4.0 high-resolution / high-sensitivity sCMOS camera and a Vivatome for fast 3D acquisition. Details of the microscopes will soon be available on our website and in our WIKI, I'll keep you posted. Installation starts this week, then the microscopes will be available to users in NHLI as well as other Imperial College users. The microscopes will be part of FILM, so you can expect the same training and support as so far on the South Kensington campus. The new FILM branch will be started with two OPENING AND TRAINING DAYS on THURSDAY+FRIDAY 14+15 JUNE 2012 THURSDAY 14 JUNE MORNING SESSION (Wolfson Building LT2): Presentations 10.00-10.10 (Prof Tony Magee, head of FILM): Welcome and opening of the L-Block branch of FILM 10.10-10.20 (Martin Spitaler, FILM): Overview and specifications of the new microscopes 10.20-11.20 (David Crook, Zeiss): Functions and applications of the widefield microscope setup (AxioObserver with Vivatome, Colibri LED illumination and Hamamatsu Flash 4.0 camera) # hardware specifications # software # high-speed acquisition (fast LED switching, fast camera options) # automated tiled, multi-position and multi-well acquisition # 3D acquisition with the Vivatome 11.20-11.30 coffee break 11.30-13.00 (Graham Brown, Zeiss): Functions and applications of the confocal microscope setup (LSM-780 Meta) # hardware specifications # confocal-specific software functions # setup of fluorophor-specific light paths, PMT versus Meta-GaAsp detectors # spectral acquisition with the Meta detector and spectral unmixing 13.00-14.00 lunch break AFTERNOON SESSION (Microscope Room L-Block 314): 14.00-17.00: hands-on session for users with their own sample (advance booking) FRIDAY: Further hands-on session for users with their own sample, according to demand To get your personal introduction with your own samples, please book your personal 2-hour slot by email to Stephen M Rothery <s.rothery@imperial.ac.uk> (this introduction will NOT replace your own personal training session, which will still be provided later) Presentations on Thursday morning will be open to everybody. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler@imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility