Dear microscopists, just a reminder TODAY is the FILM MICROSCOPY DAY - EVERYBODY IS WELCOME (you don't need to be a FILM user) There will also be a short overview of FILM for new people, and WHAT'S NEW for existing users, e.g. the new microscopes in Hammersmith, new Huygens deconvolution software (including confocal and multiphoton modules), just before the match maker session
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10.00- 10.10 Welcome by Prof Tony Magee, head of FILM GENERAL MICROSCOPY TECHNIQUES 10.10-10.55 Basics of microscopy - magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy (Martin Spitaler, FILM) 10.55-11.40 Live imaging techniques - Multiphoton, live imaging and second harmonic generation (SHG) (Mark Scott, FILM) 11.40-12.00 coffee & tea break 12.000-13.00 MICROSCOPY MATCHMAKER: FILM users giving a snapshot of their work in 2-minute presentations Introduction: FILM overview, what's new in FILM (Martin Spitaler) # David Hodson: tba # Katrin Kronenberger: Optimisation of whole mount imaging # Malak Al-Yamani & Martina Lundberg: Imaging the inside wall of the mouse aortic arch # Mirella Koleva: Study of Sonic hedgehog multimerisation # Nickolai Vysokov: Role of Latrophilin-Lasso trans-synaptic intreaction # Paulina Ciepla: tba # Sophie Pageon: Imaging the nanoscale organisation of KIR2DL1 using PALM 13.00-14.15 Poster session with sandwich lunch, ground floor (outside cafeteria) (kindly sponsored by CARL ZEISS MICROSCOPY) SPECIAL TECHNIQUES 14.15-15.00 Fluorophores as sensors - colocalisation, fluorescence lifetime (FLIM) and resonant energy transfer (FRET) (Valentina Caorsi, NHLI) 15.00-15.45 Super-resolution microscopy - TIRF, PALM, STORM (Steve Rothery, FILM) 15.45-16.05 coffee & tea break UNDERSTANDING IMAGES 16.05-16.30 Image data: data formats, pixel and metadata, image visualisation and presentation, OMERO database (Martin Spitaler, FILM) 16.30-17.35 Image analysis: noise removal, deconvolution, segmentation, tracking, registration (Luis Pizarro, Computing) 17.35-17.40 Wrap-up, feedback, outlook For more and up-to-date information, please check <http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/news>. See you all there, good imaging, Martin ############################################# -- Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler@imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility