Dear all,

Dr Valentina Caorsi, from abbelight, will give a talk on single molecule localization microscopy at FILM, as described below:

Date:                     Tuesday 23^rd October

Time:                     12.30-13.30

Room:                   G120 in SAF Building

Into the nanoworld

Breaking the resolution limit of conventional microscopy opened the way to investigation of cellular structures at the nanoscale, from individual proteins to entire organelles. Different approaches have been proposed from structured illumination microscopy (SIM) to stimulated emission depletion (STED) and single molecule localization microscopy approaches (SMLM). SMLMs, such as fluorescence photoactivated localization microscopy ((F)PALM) and direct stochastic optical reconstruction microscopy (dSTORM), can easily provide lateral localization precision down to 5–10 nm. However, 3D multicolour nanoscopy is still a challenge and a lot of effort has been made by the nano-community to develop quantitative and reproducible 3D super-localization methods.

In this panorama abbelight developed a new nanoscope allowing precise isotropic 3D localization precision (15X15X15nm) by decoupling the lateral and axial detection. For the latter, two different sources of axial information are retained: the supercritical angle fluorescence (SAF) that provides an absolute z-reference (0-600nm) and a strong astigmatism-based PSF measurement allowing an extended axial depth (up to 5mm).

Thus, nanoscopy can be accessible, to gain more details in 3D and extract quantitative information (numbers, size, distributions, spatial organization of individual molecules, clusters or organelles).

Additionally, abbelight is organising a 3-days workshop on SMLM at King’s College London (see attached poster), and there are spaces for Imperial College researchers to attend and test abbelight’s DAISY module with their own samples.

If interested, please contact Valentina: vcaorsi@abbelight.com

All the best,

David