Dear all, I want to highlight the excellent paper by James Jonkman et al. Tutorial: Guidance for quantitative confocal microscopy published yesterday in Nature Protocols: https://www.nature.com/articles/s41596-020-0313-9 It is a very comprehensive guide for confocal microscopy including sample preparation, instrumentation, image acquisition and even statistics, maybe the best ever written on this topic! Please read before using the FILM microscopes, we also included a link on our webpage under Resources/General Information. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer@imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------