Dear microscopists, the first FILM Club of the new year is coming up next week Wednesday: *Catherine Simpson: "Sort your microscopy out!" Wed 29 Jan, SAFB-122, 4.30pm* Catherine Simpson is the facility manager of the Flow Cytometry Facility in South Kensington (http://www3.imperial.ac.uk/lifesciences/research/facilities/flowcytometry). In this talk, she will go into the very powerful combination of flow cytometry and microscopy to extract much more useful information from biological samples. While flow cytometry is incredibly powerful in quantifying multiple cellular parameters at high speed from large numbers of samples, it's missing one important trick: The cellular context of these parameters in time and space. This is where microscopy excels, it keeps the whole big picture intact, how molecules relate to each other, interact with each other and change over time. But this comes at the price of often lousy statistics, especially for live imaging. And it can take ages to find the 'needle in the haystack', for example a cell of a certain type, transiently transfected or with a certain expression level or combination or fluorophores. Combining the two - analysis and sorting on a FACS, live visualisation and measurement on a microscope - opens up a whole new dimension of understanding. Cells can be analysed in high numbers, rare cells sorted into microscopy multi-well plates or slides, or even re-introduced into live animals and followed up by intravital imaging. Some people ask for the ImageStream technology to analyse their samples - we can do all that and much more, at much higher magnification and over time! See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler@imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility