Dear All, Does anyone have a red fluoresent streptavidin that I could take a couple of ul of to test for a dual staining protocol please? Many thanks, Lisa Dr Lisa Gregory Leukocyte Biology Section National Heart & Lung Institute Faculty of Medicine Imperial College London South Kensington Campus Sir Alexander Fleming Building London SW7 2AZ Tel: +44 (0)20 7594 3129 Fax: +44 (0)20 7594 3119 Email: l.gregory@imperial.ac.uk ________________________________ From: film-users-bounces@imperial.ac.uk [film-users-bounces@imperial.ac.uk] on behalf of Martin Spitaler [m.spitaler@imperial.ac.uk] Sent: 11 December 2013 09:27 To: film-users Subject: [FILM-Users 00447] TODAY: Eric Betzig lecture super-resolution and light sheet microscopy on 11th December 2013 -------- Original Message -------- Subject: Eric Betzig lecture super-resolution and light sheet micrscopy on 11th December 2013 Date: Tue, 5 Nov 2013 15:35:16 +0000 From: French, Paul (PHOT) M W (Photonics) <paul.french@imperial.ac.uk><mailto:paul.french@imperial.ac.uk> To: Spitaler, Martin <m.spitaler@imperial.ac.uk><mailto:m.spitaler@imperial.ac.uk> Dear Martin, Eric Betzig is one of the leading pioneers of both PALM microscopy for super-resolved imaging and light sheet microscopy for rapid imaging of live organisms. This promises to be an excellent talk. Please could you advertise it, e.g. through your FILM mailing list and the London super-resolution club. Best wishes, Paul Leica Scientific Forum UK presents Eric Betzig Wednesday, December 11, 2013 at 17:00h - Chair: Paul French Venue: Imperial College London, Lecture Theatre 3, Blackett Laboratory, Prince Consort Road, London SW7 2BB Eric Betzig<http://www.janelia.org/lab/betzig-lab> Janelia Farm Research Campus, Howard Hughes Medical Insitute, Ashburn, VA "Imaging Life at High Spatiotemporal Resolution" Optical microscopy has remained at the forefront of biological discovery for centuries. However, as our understanding of biological systems as increased, so has the complexity of our questions and the need for more advanced optical tools to answer them. For example, there is a hundred-fold gap between the resolution of conventional optical microscopy and the scale at which molecules self-assemble to form sub-cellular structures. Furthermore, as we attempt to peer more closely at the dynamic complexity of living systems, the actinic glare of our microscopes can adversely influence or even kill the specimens we hope to study. Finally, the heterogeneity of life, ranging from organelles within single cells to specialized cell types within tissues and organs, can seriously impede our ability to image at high resolution, due to the resulting warping and scattering of light rays. I will describe three areas focused on addressing these challenges: super-resolution microscopy for imaging specific proteins within cells down to near-molecular resolution; plane illumination microscopy using non-diffracting beams for noninvasive imaging of three-dimensional dynamics within live cells and embryos; and adaptive optics to recover optimal images from within optically heterogeneous specimens Invitation poster: <https://workspace.imperial.ac.uk/imagingfacility/Public/seminar.pdf>