- FILM-Users - imperial.ac.uk

[FILM-Users 00910] !!!!!! DELETION of files from SCRATCH2 !!!!!!!!!!!!!!!!!
by Rothery, Stephen M 08 Sep '20

08 Sep '20

Dear All The scratch2 drive is getting full, so we need to delete older files from it We will delete files older than the 1st July 2019 next week Please make sure you have everything backed up from before then Also you are reminded that the scratch drive is for transferring FILM data only It is not for long term storage or for users to store other data and programs If you are working offsite then scratch can be accessed either through VPN or using the remote gateway to logon to you work PC Many thanks Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00922] Basophils degranulation video for BBC documentary
by Bruckbauer, Andreas 04 Sep '20

04 Sep '20

Dear all, Please see Rebecca's email below for a chance to have your work shown in a BBC documentary. Please contact her directly if you can help. Best wishes Andreas From: Parkin, Rebecca V <r.parkin(a)imperial.ac.uk> Sent: 04 September 2020 16:44 To: Bruckbauer, Andreas <a.bruckbauer(a)imperial.ac.uk> Cc: Shamji, Mohamed H <m.shamji99(a)imperial.ac.uk> Subject: Basophils degranulation video Dear Andreas, I am looking for someone who has filmed basophils degranulating and would be willing to share a clip to use in a BBC documentary. Do you know of anyone who has used your facility who has done this? Many thanks Rebecca Rebecca V Parkin Senior Research Technician, Immunomodulation and Tolerance Group, Immune Tolerance Network (ITN) Distributed Centre of Excellence for Allergy & Asthma Allergy & Clinical Immunology Inflammation, Repair and Development National Heart & Lung Institute | Imperial College London Sir Alexander Fleming Building | South Kensington Campus London SW7 2AZ | United Kingdom Tel: +44 (0)20 7594 5379 | Mobile: +44 (0)7809227811 E-mail: r.parkin(a)imperial.ac.uk<mailto:r.parkin@imperial.ac.uk> Get Outlook for iOS<https://aka.ms/o0ukef>

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[FILM-Users 00921] doxorubicin request
by Bruckbauer, Andreas 04 Sep '20

04 Sep '20

Dear all, Please see the email from Foivos below, please reply to him directly in case you can help. Best wishes Andreas ---------------------------------------------------------------------------------------------------------- From: Chatzidimitriou, Foivos <f.chatzidimitriou17(a)imperial.ac.uk> Sent: 04 September 2020 12:44 To: Bruckbauer, Andreas <a.bruckbauer(a)imperial.ac.uk> Subject: doxorubicin request for mailing list Hi Andreas, I hope all is good. I wanted to do an experiment with doxorubicin today but my order from sigma hasn't arrived yet. Is there any chance you could send an email to the FILM mailing list asking whether anyone could borrow me some which I'll be happy to replace soon? Many thanks - have a nice weekend :) Foivos

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[FILM-Users 00920] Replacement to the ICTEM Analysis PC
by Rothery, Stephen M 04 Sep '20

04 Sep '20

Dear All The ICTEM analysis PC has become unusable, and as the need for remote use of the analysis PC's has increased, we have setup a second analysis PC at SAF as the new ICTEM analysis PC If you would like remote access to either or both these PC's you will need to contact a member of FILM staff, so that we can add you to the list You are reminded that currently the use of the analysis PC's is solely by remote Login The FILM analysis PC's may only be used remotely Remote connecting to the analysis computer is usually through the ICRDGateway See link below on how to connect http://www.imperial.ac.uk/admin-services/ict/self-service/connect-communica… PC Names and boking Please book the analysis pc on PPMS - no charge Hammersmith analysis pc name is dh-mspitale03 Book on ICTEM analysis page in PPMS SAF analysis PC name is FILM-skanalys02 Book on SAF analysis page in PPMS [cid:image002.png@01D682B5.482DA250] And IMPORTANT - when you finish - Make sure you log off (sign out) - DO NOT SHUT DOWN or SWICH USER And please don't save items to the desktop as this will fill up the C drive There is a D drive\User Data folder for temporarily storing data - please use this instead of the desktop NB if you need to map the scratch2 drive \\FILM-nas2.med.ic.ac.uk\scratch2<file://FILM-nas2.med.ic.ac.uk/scratch2> Right click on "This PC" and map the drive using the link above If you need to do stitching in ZEN, you may need to activate the licence: Run ZEN blue Go to Tools- Modules Manager Then tick on the Panorama module And the stitching option should appear in the methods of processing [cid:image001.png@01D65A86.394ACFD0] Any problems, please contact FILM staff Best Wishes Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00919] Microfluidic platform for a multiplex staining approach?
by Bruckbauer, Andreas 26 Aug '20

26 Aug '20

Hello, can anyone help Mitchell? Please contact him directly mitchell.pallett10(a)imperial.ac.uk<mailto:mitchell.pallett10@imperial.ac.uk> ------------------------------ Good morning, I am looking for a system to deliver small quantifies of buffers (dNTP conjugated fluorophores) to multiplex label and repeatedly wash, stain and image frozen spleen sections. Do you happen to know anyone at Imperial that is currently using a microfluidic platform for a similar multiplex staining approach? Thank you for your time and assistance. Kind regards, Mitchell Pallett Research Associate MRC CMBI, Flowers Building Imperial College London ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00918] !!!! Huygens server local drive D data deletion !!!!!!
by Rothery, Stephen M 20 Aug '20

20 Aug '20

Huygens server local drive D data deletion (FILM04) Dear all If you are a Huygens deconvolution software user - The data drive D (scratch D) on the Huygens analysis server is getting full and so we need to delete older data We will delete data from before 1st January 2020 next week, please make sure you have all your data backed up Many thanks steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00917] Virtual Light Sheet Microscopy conference
by Bruckbauer, Andreas 19 Aug '20

19 Aug '20

FYI, virtual Light Sheet Microscopy conference: I'm emailing to let you know about the virtual version of the LSFM conference that is taking place in September. I'd be very grateful if you could advertise the link and banner below to anyone you think may be interested. Meeting registration and abstract submission are now open. https://www.rms.org.uk/lsfm2020.html [cid:image001.png@01D6763E.74FA02C0] Best regards, Chris

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[FILM-Users 00916] PDRA in Microscopy of Mitochondrial Copy-Number
by Bruckbauer, Andreas 17 Aug '20

17 Aug '20

Please see this interesting PostDoc opportunity with Patrick Chinnery (Cambridge Mitochondrial Biology Unit/Clinical Neuroscience) and Nick Jones (Imperial Maths): A. ------------------------------------ PDRA in Microscopy of Mitochondrial Copy-Number https://www.imperial.ac.uk/jobs/description/NAT00744/research-associate-mic… Closing date 14 September 2020 Applications are invited for a 2-year PostDoc joint between the labs of Patrick Chinnery (Cambridge Mitochondrial Biology Unit/Clinical Neuroscience) and Nick Jones (Imperial Maths) using microscopy to study the control of mitochondrial genome copy-number. Cells control the copy-number of their mitochondrial DNA (mtDNA) yet the process of this control has been poorly characterised. Through a combination of microscopy, image analysis and some flow-cytometry the appointee will attempt to understand and perturb this system. Beyond being of fundamental relevance to our biological understanding; the character of control of mtDNA defines the rate and manner in which mutant mtDNA expands through cellular populations. The expansion of mutant mtDNA is implicated in ageing: thus the project seeks to identify some of the basic mechanisms of ageing. The project is a close collaboration between two groups that both have a track record of working at the interface between experiment and theory and aligns with another PDRA and 2 PhDs joint between our groups. Candidates might have backgrounds in quantitative biology/microscopy or experimental biological physics/biophysics. The core skills will be single-cell microscopy with some image analysis. The appointee will join the Centre for the Mathematics of Precision Healthcare, the Cambridge Mitochondrial Biology unit and a new subgrouping in Imperial Mathematics on Single Cell theory and data. We welcome candidates from all backgrounds and value a supportive environment prioritising purposeful happiness. We anticipate an ~October 2020 start. Relevant links: https://www.imperial.ac.uk/jobs/description/NAT00498/research-associate http://systems-signals.blogspot.com/ https://www.imperial.ac.uk/people/nick.jones/ http://www.mrc-mbu.cam.ac.uk/people/patrick-chinnery http://www.imperial.ac.uk/mathematics-precision-healthcare/ -- Department of Mathematics - Imperial College London

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[FILM-Users 00915] PPMS working again
by Bruckbauer, Andreas 10 Aug '20

10 Aug '20

Dear all, There were problems for some users to log onto our PPMS booking system, ICT told me that this is now resolved. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00914] !!! WF3 microscope moved to room 410 !!!
by Bruckbauer, Andreas 05 Aug '20

05 Aug '20

Dear FILM-users, We moved the WF3 microscope next door to room 410 from today (thanks Steve!). This allows to use the WF1 and WF3 simultaneously while the COVID restrictions apply. This means that booking the Nikon WF4-HC simultaneously with the WF3 will not be possible. Users who want to run longer experiments on the Nikon system should contact FILM staff. Please let us know if you experience any problems with these microscopes or room access. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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