- FILM-Users - imperial.ac.uk

[FILM-Users 00080] feedback Microscopy Day
by Martin Spitaler 21 Jun '11

21 Jun '11

Dear microscopists, thank you very much for everybody keeping their day free and joining the microscopy day, it was good seeing so many of you there. To help planning the event in the future, we would now appreciate any feedback and comments from your side. It's not easy for us to know what is interesting and useful for you, so please tell us what was missing, too short, too long, too much (or simply ok as it was). Thanks in advance (and thanks those who have already told us), and good imaging, Martin* ########################################## *Martin Spitaler, PhD ** *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00077] Reminder: MICROSCOPY DAY 2011
by Martin Spitaler 20 Jun '11

20 Jun '11

...just a little reminder for our Microscopy Day next Monday... Martin ======================================================== Dear microscopists, this year we are are having a microscopy day again, a whole day of lectures, presentations and demonstrations about microscopy: MICROSCOPY DAY, MONDAY 20 JUNE 2011, 9.45-17.00, SAFB-G34 Following up and expending on the successful event two years ago, we are offering information from the very basics to the highly sophisticated, from fluorophores to image analysis. The details are still being finalised, if you are interested in a particular topic please let me know. PRELIMINARY PROGRAMME: 9.45- 9.50 Introduction 9.50-10.30 Basics of microscopy: properties of light, diffraction limit, resolution, contrast methods, epifluorescence, confocal, ... 10.30-11.15 Principles of fluorescence: fluorophores, photo-bleaching, photo-switching, phototoxicity, ... 11.15-11.30 coffee 11.30-12.15 Special techniques I: two photon microscopy, second harmonic generation, in vivo imaging, Flim, Fret, ... 12.15-13.00 Special techniques II: high-resolution microscopy (TIRF, PALM, STORM), ... 13.00-14.30 lunch break and demonstrations: - TIRF, PALM, STORM - 2P, SHG, in vivo, Flim - tour of EM facility - OMERO image database, XperimentR - image analysis tools 14.30-15.15 Transmission electron microscopy 15.15-16.00 Understanding and handling images: data formats, pixel and metadata, visualisation, OMERO database, analysis workflow, ... 16.00-16.15 coffee 16.15-17.00 Tools for image analysis: filtering, edge detection, segmentation, feature extraction, morphoplogy, ... For more details and updates, see FILM website / NEWS (http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/news) Everybody is welcome. see you there, and good imaging, Martin ######################################### Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00078] Fwd: Euro-BioImaging: 1st Newsletter
by Martin Spitaler 17 Jun '11

17 Jun '11

Dear microscopists, I'm forwarding the information below regarding the EuroBioimaging initiative (in which we are participating). Most likely Eurobioimaging will in the future help your microscopy research in the way of training, facility access, data management and analysis, lobbying for funding and generally promoting bioimaging (see the website for more details), so you might be interested to stay up to date through this newsletter. By the way, the local initiative, BioImagingUK (http://www.bioimaginguk.org) is also taking shape, the dedicated Working Groups have already produced very interesting documents how bioimaging should be organised, supported and funded, which are going to be presented to the funding bodies (research councils and Wellcome Trust) soon. We also have a mailing list there (see bottom of the homepage) to keep you up to date. Good imaging, Martin ############################################################################ -------- Original Message -------- Subject: Euro-BioImaging: 1st Newsletter Date: Tue, 7 Jun 2011 13:10:48 +0100 From: info(a)eurobioimaging.eu <info(a)eurobioimaging.eu> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> E U R O B I O I M A G I N G Dear Martin Spitaler, We are pleased to herewith send you the first edition of the Euro-BioImaging biannual Newsletter. Don`t miss the special sections "Spread the word: The survey is on" and "Behind the scenes". < http://www.eurobioimaging.eu/content-news/1st-euro-bioimaging-newsletter-av… > Enjoy reading, The Euro-BioImaging Communication Team (WP5)

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[FILM-Users 00076] TODAY: FILM Club
by Martin Spitaler 27 May '11

27 May '11

...just a reminder about the FILM-Club TODAY, Martin > > > Dear microscopists, > > sorry for the late notice, there were some problems with the > organisation, but this Friday it's time for the FILM Club again: > > FILM Club May 2011: > Amir Horowitz: Live cell imaging of particle capturing during apoptosis > > Friday 27 May 2011, SAFB-121 > > Amir Horowitz is presenting his project which is based on a combination > of confocal imaging, 3D image analysis and mathematical modelling to > understand biological and physical mechanisms behind the process of > phagocytosis. > > This is going to be an interesting talk for many of us, either working > on phagocytosis, or generally interested in the combination of tools to > understand biology. > > > See you there, good imaging, > > Martin > > ############################### > Martin Spitaler, PhD > > FILM - Facility for Imaging by Light Microscopy > - Facility Manager - > Sir Alexander Fleming Building, desk 401 > Imperial College London / South Kensington > Exhibition Road > London SW7 2AZ > UK > > Tel. +44-(0)20-759-42023 > E-mail m.spitaler(a)imperial.ac.uk > Website: http://imperial.ac.uk/imagingfacility > > > _______________________________________________ > FILM-Users mailing list > FILM-Users(a)imperial.ac.uk > https://mailman.ic.ac.uk/mailman/listinfo/film-users

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[FILM-Users 00075] Fwd: Dyes
by Martin Spitaler 26 May '11

26 May '11

...can anyone help Florencia? If so, please contact her directly, Martin -------- Original Message -------- Subject: Dyes Date: Tue, 24 May 2011 12:09:07 +0100 From: Minuzzi, Florencia <florencia.minuzzi05(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, I’m trying to look at the ER in my cells, and I think DiOC6 is probably the best dye to use in yeast. Do you have any that I could borrow to try before I buy it? The catalogue number is D-273. Thanks, Florencia

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[FILM-Users 00074] Fwd: FILM Club this Friday
by Martin Spitaler 24 May '11

24 May '11

Sorry, I forgot the time: As usual, it's 1PM, this Friday SAFB-121, Martin -------- Original Message -------- Subject: FILM Club this Friday Date: Tue, 24 May 2011 17:47:55 +0100 From: Martin Spitaler <m.spitaler(a)imperial.ac.uk> To: film-users <film-users(a)imperial.ac.uk> Dear microscopists, sorry for the late notice, there were some problems with the organisation, but this Friday it's time for the FILM Club again: FILM Club May 2011: Amir Horowitz: Live cell imaging of particle capturing during apoptosis Friday 27 May 2011, SAFB-121 Amir Horowitz is presenting his project which is based on a combination of confocal imaging, 3D image analysis and mathematical modelling to understand biological and physical mechanisms behind the process of phagocytosis. This is going to be an interesting talk for many of us, either working on phagocytosis, or generally interested in the combination of tools to understand biology. See you there, good imaging, Martin ############################### Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00073] FILM Club this Friday
by Martin Spitaler 24 May '11

24 May '11

Dear microscopists, sorry for the late notice, there were some problems with the organisation, but this Friday it's time for the FILM Club again: FILM Club May 2011: Amir Horowitz: Live cell imaging of particle capturing during apoptosis Friday 27 May 2011, SAFB-121 Amir Horowitz is presenting his project which is based on a combination of confocal imaging, 3D image analysis and mathematical modelling to understand biological and physical mechanisms behind the process of phagocytosis. This is going to be an interesting talk for many of us, either working on phagocytosis, or generally interested in the combination of tools to understand biology. See you there, good imaging, Martin ############################### Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00072] Definiens training course
by Martin Spitaler 20 May '11

20 May '11

Dear microscopists, I'm planning to organise another training course for the Definiens advanced image analysis software this summer. If you are interested in such a course, please let me know, content and dates of the course are flexible and depend on the requirements of participants. If there is enough interest, I hope of organising a basic course for beginners and an advanced version for more experience users. The costs for the course should be ~£400 per participant. The Definiens software gives biologists relatively easy access to very complex image analysis tools. Since we installed it about a year ago, we have very successfully used it for a large range of microscopy images in the facility (the largest analysis was on a dataset of 30,000 images). Unlike Volocity, the software doesn't provide quick drag&drop solutions for simple tasks, but for anything more demanding it provides a huge set of tools to identify objects in crowded and noisy microscopy images, taking into account biological information, and extract a large range of features about intensity, shape, position, orientation, density, boundaries, .... of objects. If you are interested, please get in contact with me. If you want more information, have a look at the links on the FILM website / Equipment / Definiens (http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/equipment…) or on the Definiens Community website (http://community.definiens.com/home.html) Good imaging, Martin ################################## Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00071] Fwd: ibidi Online LIVE Webinar - 18th May...
by Martin Spitaler 17 May '11

17 May '11

...for your information, Martin -------- Original Message -------- Subject: ibidi Online LIVE Webinar - 18th May... Date: Mon, 16 May 2011 16:13:18 +0100 From: James Smith <James(a)thistlescientific.co.uk> Reply-To: James(a)thistlescientific.co.uk <James(a)thistlescientific.co.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> To view as a webpage, click here <http://www.thistlescientific.co.uk/e-news/ibidi-may/ibidi-may.html> Visit our website <http://www.thistlescientific.co.uk> Contact us <http://www.thistlescientific.co.uk/acatalog/contact_us.html> *Immunofluorescence <http://www.ibidi.de/products/disposables/S_8060X_SlideVI.html>Angiogenesis <http://www.ibidi.de/products/disposables/S_8015X_Slide_Angio.html>Flow assays <http://www.ibidi.de/products/instruments/I_109XX_ibidi_pump.html>Life Cell Imaging <http://www.ibidi.de/products/disposables/D_811XX_Dish35mm_high.html>* */Chemotaxis <http://www.ibidi.de/products/disposables/S_8030X_Slide_Chemo.html>Wound Healing <http://www.ibidi.com/products/disposables/E_802XX_CI_family.html>Gene Transfection <http://www.ibidi.de/products/reagents/R_606XX_TransfKitVI.html>/* /Live Webinar:/ /Principles and Basics of Light Microscopy for Cell-Based Assays/ /During the last decade, the imaging of cultured, or primary, cells has played a major role in helping to understand biological processes. Cell-based assays use light microscopy as one of the most dominant parameters in the visualization of multi-parameter data sets./ /In this webinar we will present the basics of microscopy starting with the inner workings of modern microscopes. We will also discuss the optical properties of glassware and Petri dishes, including the underlying physics. Finally, you will learn about the principles of fluorescence microscopy techniques and modern live cell imaging, using specially designed ibidi -Slides and -Dishes. / /**Register now for your preferred date*:*/ * // /*>> May 18, 2011, 8 am BST (3 pm Taiwan | 4 pm Tokyo | 5 pm Sydney) <http://www.ibidi.de/db_webinars/webinar_mb_20110518_8amCET.php>* / * /*>> May 18, 2011, 1 pm BST <http://www.ibidi.de/db_webinars/webinar_mb_20110518_2pmCET.php>* / * /*>> May 18, 2011, 4 pm BST (11 am EDT | 10 am CDT | 8 am PDT) <http://www.ibidi.de/db_webinars/webinar_mb_20110518_5pmCET.php>* / /*This webinar will focus on the following topics:* / * // /Basics of light microscopy (e.g. optical properties and cover slips) / * /Practical aspects and applications (e.g. Khler illumination and contrast techniques) / * /Fluorescence microscopy / * /Live cell imaging applications / /* */ /Principles and Basics of Light Microscopy for Cell-Based Assays/ /*Elias Horn, ibidi GmbH*/ /Elias Horn studied biotechnology at the University of Applied Science, Mittweida. Since 2004, he has been working on the development of customer-specific slides, for /in vivo/ cell culture experiments, in the Research and Development department at ibidi. His main focus is the design and implementation of migration and chemotaxis assays./ // /For further information on any of our products, contact 01698 338844, or to browse our catalogue visit www.thistlescientific.co.uk <http://www.thistlescientific.co.uk>./ /Thistle Scientific - DFDS House - Goldie Road - Uddingston- GLASGOW - G71 6NZ/ /TEL: 01698 338844 - FAX: 01698 338880 - MAIL: info(a)thistlescientific.co.uk <mailto:info@thistlescientific.co.uk>/ /*********************************************************************************************************************************************************** To unsubscibe click here <http://www.thistlescientific.co.uk/acatalog/newsletter-feedback.html> / /***********************************************************************************************************************************************************/ //

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[FILM-Users 00070] MICROSCOPY DAY 2011
by Martin Spitaler 17 May '11

17 May '11

Dear microscopists, this year we are are having a microscopy day again, a whole day of lectures, presentations and demonstrations about microscopy: MICROSCOPY DAY, MONDAY 20 JUNE 2011, 9.45-17.00, SAFB-G34 Following up and expending on the successful event two years ago, we are offering information from the very basics to the highly sophisticated, from fluorophores to image analysis. The details are still being finalised, if you are interested in a particular topic please let me know. PRELIMINARY PROGRAMME: 9.45- 9.50 Introduction 9.50-10.30 Basics of microscopy: properties of light, diffraction limit, resolution, contrast methods, epifluorescence, confocal, ... 10.30-11.15 Principles of fluorescence: fluorophores, photo-bleaching, photo-switching, phototoxicity, ... 11.15-11.30 coffee 11.30-12.15 Special techniques I: two photon microscopy, second harmonic generation, in vivo imaging, Flim, Fret, ... 12.15-13.00 Special techniques II: high-resolution microscopy (TIRF, PALM, STORM), ... 13.00-14.30 lunch break and demonstrations: - TIRF, PALM, STORM - 2P, SHG, in vivo, Flim - tour of EM facility - OMERO image database, XperimentR - image analysis tools 14.30-15.15 Transmission electron microscopy 15.15-16.00 Understanding and handling images: data formats, pixel and metadata, visualisation, OMERO database, analysis workflow, ... 16.00-16.15 coffee 16.15-17.00 Tools for image analysis: filtering, edge detection, segmentation, feature extraction, morphoplogy, ... For more details and updates, see FILM website / NEWS (http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/news) Everybody is welcome. see you there, and good imaging, Martin ######################################### Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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