August 2024 - FILM-Users - imperial.ac.uk

[FILM-Users 01172] Update on FILM Facility Charging Rates
by Nechyporuk-Zloy, Volodymyr 22 Aug '24

22 Aug '24

Dear Colleagues, As you know, FILM, our imaging facility, has been operating under significant economic and technological pressures, reflecting the broader situation in the UK economy. The annual inflation rates over the last four years have been as follows: - 2021: 2.6% - 2022: 9.1% - 2023: 7.3% - 2024: 3.1% (up to July) The cumulative inflation over these four years is approximately 24.2% (23.08%)<https://www.bankofengland.co.uk/monetary-policy/inflation/inflation-calcula…>: [cid:3bffd417-5ac5-42fc-9c45-0e25fd70814a] These inflationary pressures have led to increased costs in staff expenses, service contracts, consumables, and necessary investments in IT infrastructure within the SAF and ICTEM Buildings. Despite these challenges, our charging rates<https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…> have remained fixed over the last four years. We have tried to compensate by increasing our income through the acquisition of new equipment and intensifying our workload, which has resulted in income growth each year, including a 10.9% increase in 2023. However, due to limitations in staff numbers and other factors, it is no longer feasible to maintain the current charging rates. In the near future, we will be developing a new charging schema to address these challenges. Thank you for your understanding and continued support. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01171] Upcoming Leica Stellaris Applications Workshop on Autonomous Microscopy - 3rd September
by Nechyporuk-Zloy, Volodymyr 22 Aug '24

22 Aug '24

Dear Colleagues, We are pleased to announce that the Leica UK Team is organizing the next workshop in our Stellaris<https://www.leica-microsystems.com/products/confocal-microscopes/p/stellari…> applications series on Tuesday, 3rd September. The topic for this session will be “Autonomous Microscopy.” Autonomous Microscopy enables highly economical operations for daily laboratory work, allowing advanced experiments that would otherwise require significant manual effort or be impossible without automated procedures. This approach includes a rare event detection workflow powered by AI-based microscopy, which facilitates the automated detection of rare events without human intervention and the complete automation of complex microscopic workflows. In this autonomous workflow, low-resolution 2D overview images are initially generated and immediately transferred to the connected AI-based image processing system (Aivia<https://www.leica-microsystems.com/products/microscope-software/p/aivia/>). This system detects rare events, previously defined by the operator, using a pixel classifier and sends the event coordinates back to the imaging system for high-resolution and 3D data stack scanning according to the operator's specifications. The result is highly specific data on the object of interest, generated in statistically relevant quantities due to the automation process. Please find the program of the event attached. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01170] Introducing the Cell DIVE imager
by Ferreira Da Silva, Ana Maria 14 Aug '24

14 Aug '24

Dear All, We are thrilled to announce that the Cell DIVE imager<https://www.leica-microsystems.com/products/light-microscopes/p/cell-dive/> is now available for training sessions. The system is an automated microscope designed specifically for multiplexed experiments. It allows multiplexed tissue analysis for investigation of the expression of multiple biomarkers and the cellular and spatial localization of biomarker expression within a tissue. Please find attached the resources available in the Cell DIVE room (ICTEM room 304B). In addition to the imager and robot, the room is equipped with an antigen retrieval module, a sample preparation bench with reagents (excluding antibodies), a barcode printer, and an analysis PC. To give you an idea of what the Cell DIVE workflow looks like, you start by doing a scan with a 2x and a 10x objective to define the regions of interest, and then proceed to image the biomarker stainings with a 20x objective. You can use 4 antibodies (+DAPI) per round. Between each round you need to inactivate the dyes and image autofluorescence. We have indicative times of acquisition per round using the 20x imaging: · 1 cm x 1cm region (225 FOV): 12 minutes · 4.5 cm x 2 cm region (2584 FOV): 132 min (2h12) Leica recommends using validated antibodies from CST and Abcam. I've attached a 2 validated lists. For more details about Abcam antibodies suitable for the Cell DIVE workflow, please contact dafne.galindoduran(a)abcam.com<mailto:dafne.galindoduran@abcam.com>. To request a training session, please complete the First Training Request<https://ppms.eu/imperial/req/?pf=2&training=true&form=31> on PPMS. If you don't have a PPMS account yet, please request one here<https://ppms.eu/imperial/?FILM>. For any questions and further information, please don't hesitate to contact me. Best wishes, Ana ____________ Ana Ferreira da Silva, PhD FILM facility ICTEM, level 3 Hammersmith campus, Imperial College London Du Cane Road, London W12 0NN FILM website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/

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