July 2024 - FILM-Users - imperial.ac.uk

[FILM-Users 01169] HCF3 Survey - FINAL CALL FOR RESPONSES
by Tisi, Madeline S 25 Jul '24

25 Jul '24

Hi all, Just a reminder for those of you who have used the confocal system HCF3, now or in the past, to please fill out this quick survey about the research being conducted on the system - this is to send to the BBSRC who wish to know of developments arising from their ALERT funding; of which we were awarded to fund the HCF3 system. If you have used both HCF2 and HCF3 for your research, please also answer the survey. Thank you to those of you who have responded so far. The survey will take less than 5 minutes and may be recorded anonymously if you so wish, though we would love as much detail as possible as his will help us gain insight into user demand/areas of interest. Email addresses are not being recorded. FINAL CALL FOR RESPONSES FRIDAY 26TH JULY Please find the Google form below: https://forms.gle/zoriftqtbFSwMLom9 Thank you in advance for your time! Best wishes Maddy Maddy Tisi, BSc(Hons) PhD Advanced Microscopy Specialist Facility of Light Imaging Microscopy (FILM) National Heart and Lung Institute ____________________________________ Imperial College London ICTEM Building The Hammersmith Hospital, Du Cane Road, London W12 0NN FILM Website: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop… LinkedIn: www.linkedin.com/in/madeline-tisi<http://www.linkedin.com/in/madeline-tisi> [cid:ec2eadaa-a82a-4a8c-81f8-5c61c354e611]

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[FILM-Users 01168] Opportunity: Laboratory Manager and Senior Technician Role (Temporary)
by Nechyporuk-Zloy, Volodymyr 22 Jul '24

22 Jul '24

Dear Colleagues, We are pleased to inform you about a new opportunity available starting from November for a temporary cover for the post of Laboratory Manager and Senior Technician. This role will be primarily based in the ICTEM Building, focusing on supporting advanced imaging systems, including confocal microscopes HCF1, HCF2, HCF3, and others. The postholder will be expected to continue the implementation of STED and FLIM technologies for FILM users. This is a relatively unique opportunity to work hands-on with leading-edge imaging technologies and gain valuable experience in communicating with a broad user community. If you are interested in this opportunity, please do not hesitate to reach out to me. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01167] Update on Microscope Usage Limits at FILM Facility
by Nechyporuk-Zloy, Volodymyr 22 Jul '24

22 Jul '24

Dear FILM Users, We are pleased to announce updates to our microscope usage policy aimed at improving your imaging experience. Based on the available resources, we have decided to remove the weekly limits on microscope usage while maintaining daily limits. This change will help users with long-term projects maximize their data collection without delays. Below is a summary of the new usage limits: Resource Current Weekly Limit Current Daily Limit New Weekly Limit New Daily Limit CF3 - Leica SP5 MP/FLIM upright 20 12 0 12 CF4 - Leica SP5 MP/FLIM inverted 12 6 0 6 CF6 - Leica SP8 inverted 8 4 0 6 HCF1 - Zeiss LSM-780 inverted 0 6 0 6 HCF2 - Leica Stellaris 8 inverted 0 6 0 6 HCF3 - Leica Stellaris 8 STED-FALCON 0 6 0 6 HMI1 - Leica Cell DIVE Multiplex Imager - - 0 0 HWF1 - Zeiss Axio Observer 8 5 0 6 HWF4-HC - Nikon High Content 36 12 0 12 Imaris Software (HIVE1) 0 0 0 0 LS1 - Leica Stellaris 5 Light Sheet 16 0 0 0 SR1 - Zeiss Elyra PS.1 20 12 0 12 WF1 - Zeiss Axio Observer 12 10 0 10 WF3 - Zeiss Axio Observer 12 10 0 10 WF5 - Zeiss Axio Observer 7 12 10 0 10 WF6 - Zeiss Cell Discoverer 7 0 0 0 0 This new policy was successfully tested in the ICTEM part of the FILM facility. We remain flexible and open to adjustments based on your feedback and demand. If there is a need to reinstate weekly limits, we will reassess accordingly. We also want to remind you that bookings for the microscopes are still limited to two weeks in advance. If you have any questions or need further information, please feel free to reach out. Best regards, Volodymyr

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[FILM-Users 01166] HCF3 User Survey for BBSCRC
by Tisi, Madeline S 22 Jul '24

22 Jul '24

Hi all, We kindly ask that those of you who have used the confocal system HCF3, now or in the past, fill out this quick survey about the research being conducted on the system - this is to send to the BBSRC who wish to know of developments arising from their ALERT funding; of which we were awarded to fund the HCF3 system. If you have used both HCF2 and HCF3 for your research, please also answer the survey. The survey will take less than 5 minutes and may be recorded anonymously if you so wish, though we would love as much detail as possible as his will help us gain insight into user demand/areas of interest. Email addresses are not being recorded. Please find the Google form below: https://forms.gle/zoriftqtbFSwMLom9 Thank you in advance for your time! Best wishes Maddy Maddy Tisi, BSc(Hons) PhD Advanced Microscopy Specialist Facility of Light Imaging Microscopy (FILM) National Heart and Lung Institute ____________________________________ Imperial College London ICTEM Building The Hammersmith Hospital, Du Cane Road, London W12 0NN FILM Website: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop… LinkedIn: www.linkedin.com/in/madeline-tisi<http://www.linkedin.com/in/madeline-tisi> [cid:ea41da1d-5d68-455b-96c7-b9c69527d283]

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[FILM-Users 01165] FILM news in SAF
by Gaboriau, David C A 19 Jul '24

19 Jul '24

Dear users, >From next week I will be working from home two days a week, usually on Mondays and Wednesdays, to concentrate on administrative tasks. I will be able to offer support remotely by email. I will schedule trainings for two other days, while the last day I will be concentrating on IT/software issues that need solving. I will let you know once this is done. You might also have noticed that rooms 408, 409 and 410 (as well as room 517 where the Elyra is) are now booked for cleaning every Tuesday from 6am-9am. If you have an overnight imaging session running at this time, you will have to stick a sign to the door so the cleaners do no enter and disturb your experiment. I made three signs that are located on my desk. Many thanks for your cooperation and happy imaging! All the best, David ---------------------------------------------------- David Gaboriau, PhD (Cantab), FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ https://www.imperial.ac.uk/Stories/light-fantastic/ ----------------------------------------------------

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[FILM-Users 01164] ONI super resolution microscope seminar and demo in SAF!
by Gaboriau, David C A 17 Jul '24

17 Jul '24

Dear users, Please see the message below about a talk on the ONI super-resolution microscope and an upcoming demo. All the best, David ---------------------------------------------------- David Gaboriau, PhD (Cantab), FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ https://www.imperial.ac.uk/Stories/light-fantastic/ ---------------------------------------------------- ________________________________ From: Kelwick, Richard J R <r.kelwick(a)imperial.ac.uk> Sent: 17 July 2024 17:27 To: structural-and-synthetic-biology <structural-and-synthetic-biology(a)imperial.ac.uk> Subject: ONI super resolution microscope seminar and demo in SAF! Dear all, We are hosting a demo of the ONI Nanoimager, a benchtop super-resolution microscope. Applications include: cellular imaging/phenotyping, extracellular vesicles and more. See https://oni.bio/nanoimager/ Please contact Pip Timmins phillipa(a)oni.bio<mailto:phillipa@oni.bio> at ONI if you have any questions about the microscope. The demo will be based in SAF level 2 Lab Room 245 starting Monday 22nd July and there will also be a seminar and introduction to the system on Tuesday the 23rd of July 10am-11:30am in SAF lecture room G34. See attached flyer!!! If you would like to test your own samples on the microscope please fill in the form so that ONI can organise the demo and advise on sample prep. Samples will need approval before the demo so please fill in the form as soon as possible. Demo form: Here<https://docs.google.com/forms/d/e/1FAIpQLSeihJfGw-l3-HgtPSd_vDQl5fuJ5ZUWmlN…> Thanks, Richard Dr Richard J. R. Kelwick Senior Research Associate & RSE Enterprise Fellow Alumni Imperial College London Department of Infectious Disease Section of Structural and Synthetic Biology Sir Alexander Fleming Building South Kensington Campus Exhibition Road, London, SW7 2AZ, UK Member of Imperial Postdocs and Fellows Enterprise Network (IPFEN<https://www.imperial.ac.uk/postdoc-fellows-development-centre/networks-/ipf…>) Committee [signature_1912115680]@rkelwick<https://twitter.com/rkelwick> [signature_2296782183] http://www.linkedin.com/in/richardkelwick Google Scholar<https://scholar.google.co.uk/citations?user=rFzQd_4AAAAJ&hl=en> The contents of this E-mail are confidential.

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[FILM-Users 01163] Important! OBJECTIVE CLEANING
by Tisi, Madeline S 10 Jul '24

10 Jul '24

Hi all, We have received numerous complaints about oil objectives being left in poor conditions. You should all have been shown how to clean an objective and if you have not please ask FILM staff, who will go through the procedure below with you: 1. use a low-lint lens tissue produced especially for cleaning optics, or a cotton swab - all FILM microscopes have the materials required adjacent to the microscopes. 2. if using lens tissue, fold the lens tissue to form a stiff corner, and then add 70% ethanol to the area 3. clean the objective from the centre outwards in a spiral motion, if you have put too much oil on and you have drenched the entire objective please ensure you clean this us as it is not fair to the next user. The amount of oil required should not exceed more than double the size of the objective aperture. 4. Dispose of the lens tissue/swab and repeat until all oil residue is removed. [Steps for cleaning objective lenses - use a spiral motion, starting from the rim of the lens and moving to the centre.] Unfortunately, if the objectives keep being left in these conditions we will begin charging cleaning fees. Cleaning the objectives should be part of the shut down procedure: 1. close software/shut down as you have been instructed 2. remove sample 3. clean objective 4. escape objective to lower z limit 5. change to lowest magnification objective 6. ensure condenser is enclosed and you have not left any mess All in all, this takes minutes so please be considerate! Best wishes Maddy Maddy Tisi Advanced Microscopy Specialist Facility of Light Imaging Microscopy (FILM) National Heart and Lung Institute ____________________________________ Imperial College London ICTEM Building The Hammersmith Hospital, Du Cane Road, London W12 0NN FILM Website: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop… LinkedIn: www.linkedin.com/in/madeline-tisi<http://www.linkedin.com/in/madeline-tisi> [cid:42a79790-9e74-42f0-a6de-c8a08e28b220]

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[FILM-Users 01162] Important Reminder: Proper Handling of Stage Inserts
by Nechyporuk-Zloy, Volodymyr 08 Jul '24

08 Jul '24

Dear Colleagues and FILM Users, We always consider the support and service to such a diverse and broad community of scientists as a great privilege and opportunity for us to grow in all aspects. We hope that FILM staff can support you to the best of our capability and that you can obtain excellent results here. This all requires mutual respect and understanding. In my previous email, I kindly asked our community to avoid damaging the stage inserts, as they cost more than £1,100 each, and we must cover these expenses from our own budget. Unfortunately, we have recently experienced another instance of damage to the stage insert dedicated to well-plate imaging. As you can see from the attached picture, there is substantial damage to this mechanical-optical element: [cid:e3d30cc5-5c1c-46ff-97f7-22f279970692] Optics and microscopy do not require forceful handling. Please avoid such actions and respect our equipment and work. Your cooperation is essential in maintaining the quality and availability of our resources for all users. Thank you for your understanding and support. Warm regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01161] WF1 move to ICTEM delayed until October 2024
by Gaboriau, David C A 08 Jul '24

08 Jul '24

Dear users, Some of you wrote to use asking to keep WF1 in SAF building, and as the move from NHLI to Hammersmith is slightly delayed, we will keep WF1 in SAF until October 2024 at least. All the best, David ---------------------------------------------------- David Gaboriau, PhD (Cantab), FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ https://www.imperial.ac.uk/Stories/light-fantastic/ ----------------------------------------------------

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[FILM-Users 01160] Leica Applications Workshop Series: LASX Navigator – Thursday 18th July
by Nechyporuk-Zloy, Volodymyr 08 Jul '24

08 Jul '24

Leica Applications Workshop Series – Thursday 18th July Topic: LASX Navigator Overview: LAS X Navigator is a powerful tool for sample navigation and image acquisition that allows users to generate a fast overview image and identify important specimen details instantly. Like a GPS for samples, it provides a clear path to high-quality data, enabling users to navigate through samples, create fast overviews, identify details instantly, and run high-resolution image acquisition using templates for slides, dishes, and multi-well plates. This workshop offers an excellent opportunity for attendees to bring their own samples and receive support for their specific applications. Program: 10:30 – 11:00: Navigator Overview – Teams-based Webinar (Link provided upon registration) Workshop Sessions – Leica STELLARIS 8 and Navigator Operation: - Session One: 13:00-14:00 - Session Two: 14:15-15:15 - Session Three: 15:30-16:30 All Sessions are identical Location: Bessemer Building B118, South Kensington To register for a workshop session, please send details of the applications you are working with and your Lab/Group affiliation to: emmanuelle.steib(a)leica-microsystems.com.

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