May 2024 - FILM-Users - imperial.ac.uk

[FILM-Users 01153] Notice of Limited FILM Staff Support (June 4-7)
by Nechyporuk-Zloy, Volodymyr 30 May '24

30 May '24

Dear Colleagues, We would like to inform you that from June 4th to 7th, there will be limited support from our FILM staff, as two of our team members will be attending the annual meeting of the European Light Microscopy Initiative<https://www.elmi2024.org/> in Liverpool. I will attempt to monitor and respond to emails during this period. Thank you for your understanding. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01152] Update on The Confocal System Usage for Summer: HCF1, HCF2, HCF3
by Nechyporuk-Zloy, Volodymyr 23 May '24

23 May '24

Dear Colleagues, I hope this message finds you well. To enhance access to the confocal systems in the ICTEM Building, viz. HCF1, HCF2, HCF3, during the summer months (May-August), we are making some adjustments to the usage policy. Effective immediately, we will remove the weekly usage limit while maintaining a daily limit of 6 hours. This change aims to improve accessibility and ensure fair usage among all users. Please note that these adjustments are provisional. Based on your feedback and any technical issues that may arise, we are open to revising this policy or reverting to the previous settings. Thank you for your cooperation and understanding. We look forward to your feedback to better tailor these arrangements to our community's needs. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01151] Leica workshop: TauSense Imaging:
by Nechyporuk-Zloy, Volodymyr 15 May '24

15 May '24

Dear Colleagues, I am pleased to inform you that on Thursday, 23rd May, Leica will host the first in a series of application-based workshops at the Leica Imaging Hub<https://www.imperial.ac.uk/bioengineering/about/facilities-and-services/bio…>, located in Bessemer Building B118, South Kensington. Topic – TauSense Imaging: TauSense provides dynamic access to Fluorescence Lifetime 'Functional Imaging' information in real time and in parallel with fluorescence intensity images. Utilize lifetime-based information to explore molecular functions within a cellular context, enhance image quality, and distinguish fluorescent species beyond spectral methods. Workshop Highlights: 1. Differentiate between desired and unwanted fluorescence signals, e.g., remove Autofluorescence or reflected light (TauGating, GateScan). 2. Separate fluorophores using lifetime-based information, e.g., visualize more fluorophores, eliminate cross-excitation (TauSeparation, TauScan). 3. Highlight regions of FRET activity or mean lifetime changes (Functional Imaging, Bio Sensors). 4. Directly visualize distinct structures based on lifetime differences (TauContrast), e.g., 1 detector imaging 2 or 3 lifetime-based channels – Multiplexing, Spatial Biology. Program: 9:30 – 10:00 AM: TauSense Overview – Teams-based Webinar (Link provided upon registration) Workshop Sessions on Leica STELLARIS 8 and TauSense Operation: Session One: 10:30 AM - 12:00 PM Session Two: 1:00 PM - 2:30 PM Session Three: 2:30 PM - 4:00 PM To reserve your spot, please contact Emmanuelle Steib at emmanuelle.steib(a)leica-microsystems.com. Best regards, Volodymyr

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