May 2023 - FILM-Users - imperial.ac.uk

[FILM-Users 01098] FILM: limited support on 31.05.2023
by Nechyporuk-Zloy, Volodymyr 30 May '23

30 May '23

Dear Colleagues, It is with regret that we communicate the unavailability of full on-site support for tomorrow, due to the impending industrial action within the rail system. In view of these circumstances, we will be providing limited online support during this period. We appreciate your understanding and cooperation during this time. Should you have any urgent matters that require immediate attention, please do not hesitate to reach out to us. Best regards, Volodymyr

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[FILM-Users 01097] !!!!!!!!!!!!!! DATA DELETION SCRATCH2 !!!!!!!!!!!!!!!!!!!!!!!
by Rothery, Stephen M 16 May '23

16 May '23

Dear All The scratch2 drive is getting full, so we need to delete older files from it We will delete files older than the 1st September 2022 next week NB This will include data files (eg exel data etc) Please make sure you have everything backed up from before then Also you are reminded that the scratch drive is for transferring FILM data only It is not for long term storage or for users to store other data and programs If you are working offsite then scratch can be accessed either through VPN or using the remote gateway to logon to you work PC Many thanks Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 01096] FILM: limited support on 12.05.2023
by Nechyporuk-Zloy, Volodymyr 11 May '23

11 May '23

Dear Colleagues, We regret to inform you that we will be providing only limited online support in light of the upcoming rail industrial action. https://www.nationalrail.co.uk/travel-information/industrial-action/ We apologize for any inconvenience this may cause. Best regards, Volodymyr

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[FILM-Users 01095] NIKON seminar: NSPARC - new super resolution detector
by Nechyporuk-Zloy, Volodymyr 09 May '23

09 May '23

Dear Colleagues, We would like to invite you to a NIKON seminar dedicated to the new super-resolution confocal microscopy (11.05.2023 at 14:00; SAF 113, Sir Alexander Fleming Building): https://www.imperial.ac.uk/events/162351/reveal-new-confocal-imaging-potent… Confocal laser scanning microscopy (CLSM) is an indispensable technology throughout the scientific community. By its principle, CLSM allows fluorophore emission detection (typically by a single-element detector like a photomultiplier tube) passing through a pinhole to reject out-of-focus light. By this means, the pinhole (usually set at 1AU) is primarily known for providing an optical section and therefore an improvement in the image signal-to-noise ratio (S/N). Closing the pinhole yields a resolution improvement but with a trade-off of heavy signal loss causing a reduction of the image S/N. To overcome the compromise between an increase in resolution and S/N, Nikon developed a new detector for AX igniting new confocal imaging potential. Based on the imaging scanning microscopy (ISM) technology, Nikon SPatial ARray Confocal detector (NSPARC) equipped with an array detector allows outstanding acquisition of super-resolved (SR) images equivalent to 0.2AU with S/N corresponding to 1AU. NSPARC detector can be a standalone detector or integrated with an additional confocal detector unit bringing extremely flexible hardware configuration. Combined with AX featuring the largest field of view in the market and gentle fast acquisition, NSPARC provides a simple acquisition, and unprecedented SR image quality, beyond confocal imaging. The seminar is a part of our NIKON demo: https://www.imperial.ac.uk/events/161521/nikon-confocal/ We still have a few available time slots in case you want to test the system and obtain more data. Thank you in advance for your attention, Volodymyr

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[FILM-Users 01094] Antibody request
by Rothery, Stephen M 02 May '23

02 May '23

Dear All Aanya Hirdaramani, PhD student with Prof Aylin Hanyaloglu at IRDB, is looking for a few ul of the following antibodies (all to react w human thank you): * anti-cleaved / activated notch * Hes1 * phospho and total Akt * IGF-1 * GLP-2 If you are able to spare some antibody, please contact her directly. Hirdaramani, Aanya aanya.hirdaramani15(a)imperial.ac.uk<mailto:aanya.hirdaramani15@imperial.ac.uk> Many thanks, DO NOT REPLY TO THIS E_MAIL Bw steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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