October 2023 - FILM-Users - imperial.ac.uk

[FILM-Users 01126] Update on FILM: Staffing, New Initiatives, and Opportunities
by Nechyporuk-Zloy, Volodymyr 25 Oct '23

25 Oct '23

Dear Colleagues, I hope this message finds you well. I am writing to update you on the current status and future initiatives of the FILM imaging family. Staffing Levels For the past several months, David and I have been operating at 50% of our normal staffing levels. Despite this, we have been working 50-60 hours per week to meet your imaging needs. We apologize for any delays you may have experienced in training or consultation and appreciate your understanding as we work through these challenging times. To alleviate the workload, we are actively recruiting new staff and hope to return to a normal operating schedule in the near future. Call for Support We would greatly appreciate your support in these times. If you have more than one year of experience in advanced imaging or image analysis coding and would like to further your skills in user training and support, we invite you to join us as paid Casual Workers. Instrumental Portfolio & Future Plans Currently, FILM serves an extensive community—approximately 460 users across 132 scientific groups—with diverse imaging requirements. We are committed to meeting these varied needs to the best of our abilities. Over the past 7-8 months, we have conducted 4-5 demos and equipment tests to ensure that we are offering cutting-edge technologies. Based on a recent PI survey, we have identified top priorities for future technological development. One exciting update is the forthcoming installation of multiplex imaging capabilities. Additionally, we look forward to expanding our facilities with three new rooms in the ICTEM building come February, where some equipment from the SAF building will be relocated. Special Announcement: Super-Resolution Microscopy We are pleased to announce that we are promoting the use of super-resolution microscopy. As part of this initiative, we are introducing a flexible charging policy and reducing the rate for the Zeiss ELYRA microscope to £25/hour. We have also made significant advances in STED imaging techniques, achieving resolutions as fine as 60 nm and 90 nm depending on the depletion wavelength used. Attached you will find SIM images obtained by David and STED images obtained by the Leica team and myself. We thank you for your continued support and look forward to your active participation in enhancing the FILM community. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01125] Issues with HCF2 Microscope and Call for Feedback on HCF3 Image Quality
by Nechyporuk-Zloy, Volodymyr 23 Oct '23

23 Oct '23

Dear Colleagues, I hope this email finds you well. I would like to bring to your attention some important updates concerning our imaging facilities. Unfortunately, our HCF2 microscope is currently out of service due to a software issue affecting the hardware server. I have spent 5–6 hours trying to resolve the problem but without success. I have already reached out to the Leica Microsystems team for assistance, and they are currently working on diagnosing and fixing the issue. I will keep you updated on the progress, and I sincerely hope that the microscope will be back in operation as soon as possible. In addition to this, we have received 2–3 complaints stating that the HCF3 microscope does not produce the same quality of images as the HCF2 system. If you have experienced similar issues, we would greatly appreciate your feedback. Your input will be invaluable as we work to improve the performance of both systems. If you would like to contribute to this effort, please upload your comparative data to the following folder: scratch2\_Service\_Issues\HCF3\HCF2\_HCF3\_Comparison. Your cooperation in this matter will help us make necessary adjustments and ensure that both systems meet our standards of quality. Thank you for your understanding and cooperation. I apologize for any inconvenience this may have caused and assure you that we are doing our best to resolve these issues promptly. Best regards, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…

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[FILM-Users 01124] FILM: Hyper-plex microscopy to our community.
by Nechyporuk-Zloy, Volodymyr 20 Oct '23

20 Oct '23

Dear FILM users, We are excited to welcome the Leica Cell Dive<https://www.leica-microsystems.com/products/light-microscopes/p/cell-dive/> into FILM during the coming weeks, bringing hyper-plex microscopy to our community. So we can best arrange info sessions and trainings, could you please reply to me if you are interested in using this technology in the next 6-12 months. When you reply, please indicate in your email which group you are in (PI name) and what campus you are based at. Thank you, and happy imaging, Volodymyr Dr. Volodymyr Nechyporuk-Zloy (FILM Facility Manager) Facility for Imaging by Light Microscopy Imperial College of Science, Technology and Medicine Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK Mobile: (+44) 7526275617 Twitter: @vnzloy LinkedIn: https://www.linkedin.com/in/volodymyr-nechyporuk-zloy/ Company Web: https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop… Personal Homepage: microscopist.uk<http://microscopist.uk>

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[FILM-Users 01123] Help request: purified p53 protein sample
by Gaboriau, David C A 20 Oct '23

20 Oct '23

Dear users, If anyone is able to help Jacopo Gabrielli, he is looking for a sample of purified p53 protein as a Western blot control. Please reply directly to Jacopo at jg2721(a)ic.ac.uk Please do not reply to this email. Many thanks! All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01122] Antibody request
by Gaboriau, David C A 06 Oct '23

06 Oct '23

Dear users, Please see below, if you can help Sam? Do not reply directly to this email, please contact Sam directly at s.reitemeier(a)imperial.ac.uk --------------- Hello everyone, Does anyone have access to any of these antibodies/stains? Anti-Cardiac Troponin I Antibody (ab47003) https://www.abcam.com/products/primary-antibodies/cardiac-troponin-i-antibo… Cell Signaling Technology Phopho-Troponin I (Cardiac) (Ser23/24) Antibody https://www.fishersci.com/shop/products/polyclonal-antibody-100-ul-pho-143/… Pro-Q™ Diamond Phosphoprotein Gel Stain https://www.thermofisher.com/order/catalog/product/P33300?gclid=CjwKCAjwvfm… Pro-Q™ Diamond Phosphoprotein Gel Destaining Solution https://www.fishersci.co.uk/shop/products/molecular-probes-pro-q-diamond-ph… Please feel free to email me at sreiteme(a)ic.ac.uk<mailto:sreiteme@ic.ac.uk> if you have any of these available. Thank you very much for your help. Warm regards, Sam ------------------ All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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