March 2022 - FILM-Users - imperial.ac.uk

[FILM-Users 01019] FW: AlexaFlours for IgM
by Rothery, Stephen M 29 Mar '22

29 Mar '22

Dear All If anyone can help Callum - see below, please contact him directly Muirhead, Callum r.muirhead(a)imperial.ac.uk<mailto:r.muirhead@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw Steve ----------------------------------------------------------------------------------------------------------------------------------- Hi Stephen, Do you have any AlexaFluor's that would work on IgM antibodies, any wavelength is acceptable but it would need to work with a mouse primary. Best wishes, Callum.M (he/him/they) Research Technician UK Dementia Research Institute Imperial College London 7th Floor - Sir Michael Uren Building 86 Wood Lane London W12 0BZ [cid:38cadc54-b0dd-43b2-8797-97b2431e0012]

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[FILM-Users 01018] Last chance to register your samples: Cell Discover 7 demo April 6th – 8th
by Bruckbauer, Andreas 21 Mar '22

21 Mar '22

Dear FILM-users Please let me know as soon as possible if you want to test this microscope with your sample! I need to finalize the schedule. ---------------- Zeiss offered us an on-site demo of their Cell Discover 7 microscope on April 6th – 8th at South Kensington. This is a high-end widefield system which also has a LSM 900 confocal unit with Airyscan2 (super-resolution, or live cell imaging). https://www.zeiss.com/microscopy/int/products/imaging-systems/celldiscovere… It should also allow automated workflows like overview imaging in widefield mode, locating objects of interest in x,y and z, followed by confocal imaging of these objects. The reason why we are interested in this is that they claim it is possible to image organoids at high resolution and in an automated workflow with this instrument. While there are limitations doing this with confocal microscopy and I would think this would be better done on a special light-sheet microscope, this demo could give us some answers. The positive aspect is that this is an all-in one box microscope, so it should be suitable for containment-level work (but not during the demo). I will also work for other samples like cells on coverslips, in dishes or on plates. Please let me know if you want to have your samples imaged during the demo which will take place in the SAF building at South Kensington. The time is limited (only 3days). Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 01017] FW: Antibody help request
by Rothery, Stephen M 16 Mar '22

16 Mar '22

Dear All If anyone can help Anne, please contact her directly Wolfes, Anne C <a.wolfes(a)imperial.ac.uk<mailto:a.wolfes@imperial.ac.uk>> DO NOT REPLY TO THIS E_MAIL Bw Steve -----Original Message----- From: Wolfes, Anne C <a.wolfes(a)imperial.ac.uk> Sent: 16 March 2022 15:33 To: Rothery, Stephen M <s.rothery(a)imperial.ac.uk> Subject: Antibody help request Hi Steve, I’m looking for an antibody for immunostaining fixed tissue against any of the following (NOT raised in mouse): - laminin B1 - neurexin 3a - Gapdh Any recommendations, example images, or even a small aliquot would be much appreciated! My goal is to stain the extracellular vs intracellular space in the suprachiasmatic nucleus, and the above options appear to be consistently expressed (i.e. levels don’t change during the day vs night). Many thanks, Anne

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[FILM-Users 01016] Cell Discover 7 demo April 6th – 8th
by Bruckbauer, Andreas 09 Mar '22

09 Mar '22

Dear FILM-users Zeiss offered us an on-site demo of their Cell Discover 7 microscope on April 6th – 8th at South Kensington. This is a high-end widefield system which also has a LSM 900 confocal unit with Airyscan2 (super-resolution, or live cell imaging). https://www.zeiss.com/microscopy/int/products/imaging-systems/celldiscovere… It should also allow automated workflows like overview imaging in widefield mode, locating objects of interest in x,y and z, followed by confocal imaging of these objects. The reason why we are interested in this is that they claim it is possible to image organoids at high resolution and in an automated workflow with this instrument. While there are limitations doing this with confocal microscopy and I would think this would be better done on a special light-sheet microscope, this demo could give us some answers. The positive aspect is that this is an all-in one box microscope, so it should be suitable for containment-level work (but not during the demo). I will also work for other samples like cells on coverslips, in dishes or on plates. Please let me know if you want to have your samples imaged during the demo which will take place in the SAF building at South Kensington. The time is limited (only 2.5 days) and will it takes 2 days to set up the microscope and ½ day to pack up. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 01015] Immersion oil bottle missing from CF6
by Gaboriau, David C A 09 Mar '22

09 Mar '22

Dear all, We're missing the immersion oil bottle for the CF6 confocal, if someone borrowed it or took it by mistake please let me know! Many thanks, All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS FILM Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01014] FW: Help please
by Rothery, Stephen M 08 Mar '22

08 Mar '22

Dear All If anyone can help Aida, please contact her directly Di Gregorio Pena, Aida aida.digregorio(a)imperial.ac.uk<mailto:aida.digregorio@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw Steve Dear Stephen, Could you please ask the FILM users if anyone has a spare 2uL of a secondary antibody; Alexa 647 anti rat? Many thanks, Aida

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[FILM-Users 01013] Update of COVID rules for the FILM facility
by Bruckbauer, Andreas 07 Mar '22

07 Mar '22

Dear FILM users, After speaking to our Health and Safety advisor we have made some changes to our COVID-19 rules for the FILM facility. * As it is now believed that virus transmission through contact with surfaces is less of a problem, we will remove the cling-film from keyboards and eye-pieces. We keep the 70% ethanol so that you can still clean the surfaces you touch. * We will remove the 30 min break between the sessions as we believe that our rooms are well ventilated. We keep the occupancy limit, general 1 m distancing and the requirement to wear face coverings to be on the safe side. The new rules are attached and will be presented when you book a microscope on PPMS. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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