October 2022 - FILM-Users - imperial.ac.uk

[FILM-Users 01055] FW: Non-treated 6-well plate
by Rothery, Stephen M 27 Oct '22

27 Oct '22

Dear All If anyone can help Amina - see below, please contact her directly ABULIMITI, AMINA a.abulimiti21(a)imperial.ac.uk<mailto:a.abulimiti21@imperial.ac.uk> DO NOT REPLY TO THIS E_MAIL Bw Steve From: ABULIMITI, AMINA <a.abulimiti21(a)imperial.ac.uk> Sent: 27 October 2022 13:37 To: Rothery, Stephen M <s.rothery(a)imperial.ac.uk> Subject: Non-treated 6-well plate Hi Steve, I am looking for a non-treated 6-well plate. Would you mind circulating this email in the building to see if anyone has some to spare? Thank you very much in advance! Best, Amina - Amina Abulimiti PhD student Department of Brain Sciences Imperial College London Burlington Danes, Hammersmith Hospital London W12 0NN UK

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[FILM-Users 01054] Request for antibodies
by Gaboriau, David C A 27 Oct '22

27 Oct '22

Dear Users, Please see below a request for antibodies, if you can help please reply to Antonia directly a.akoumianaki(a)imperial.ac.uk All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Interim FILM Facility Manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- Hi David, I hope all is well. Please, could you circulate the below request for a secondary antibody to FILM users for me? I would like to borrow 2ul of either Goat Anti-Mouse IgG (Alexa Fluor 488) or Goat Anti-Mouse IgG (Alexa Fluor 647) Thanking you in advance for your help. Best, Antonia

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[FILM-Users 01053] Wellcome Bioimaging Landscape Review
by Gaboriau, David C A 14 Oct '22

14 Oct '22

Dear Users, Please see below about a survey of the field of bioimaging, and the link to take the survey: https://www.research.net/r/55TLDXF?utm_campaign=2227811_October%20Research%… [https://www.research.net/collect/images/default_custom_meta_image.jpg]<https://www.research.net/r/55TLDXF?utm_campaign=2227811_October%20Research%…> Wellcome Bioimaging Landscape Review<https://www.research.net/r/55TLDXF?utm_campaign=2227811_October%20Research%…> Take this survey powered by surveymonkey.com. Create your own surveys for free. www.research.net Landscape review of barriers affecting progress in the field of Bioimaging Technopolis<https://www.technopolis-group.com/> is carrying out a global landscape review on behalf of Wellcome<https://wellcome.org/> to identify new key areas that have the potential to transform the field of bioimaging. The review will cover bioimaging methodologies, tools and technology development across the scales of life – from atoms all the way to humans – that are applicable to discovery research. An important component of the review is a stakeholder survey. Your participation in this survey will help us to understand: * What are the key emerging bioimaging technologies/methodologies? * What are the main barriers (with a specific focus on novel technology development) to progress and adoption of bioimaging technologies/methodologies? We welcome contributions from all researchers and scientists working in fields related to bioimaging, from academics and methodology/technology developers to infrastructure staff. To that end, please feel free to forward the survey link to colleagues, technical staff, postdoctoral researchers and postgraduate/doctoral students. Wellcome will use the outputs of this survey to inform its future strategy with regard to supporting bold, creative and high-quality research activities in the field of bioimaging. The survey will close on 31st October 2022. All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Interim FILM Facility Manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 01052] Fw: Antibody request please
by Gaboriau, David C A 14 Oct '22

14 Oct '22

Dear Users, Please see below for an antibody request. If you can help Annabelle please reply to a.milner(a)imperial.ac.uk All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS Interim FILM Facility Manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- ________________________________ From: Milner, Annabelle <a.milner(a)imperial.ac.uk> Sent: 14 October 2022 11:26 To: Gaboriau, David C A <d.gaboriau(a)imperial.ac.uk> Subject: Antibody request please Dear David I hope all is well. I'm currently running a pilot experiment looking at microvilli expression in Caco-2 cells. Please may you circulate the below request for a primary antibody (not raised in mouse) to FILM users for me? I'm looking to borrow 3-4 uL of either -Villin -Fimbrin/plastin 1 -ESPN -Ezrin Or any other antibody that would be recommended for looking at microvilli Many thanks! Annabelle

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[FILM-Users 01051] FW: Bcl-2 antibody
by Rothery, Stephen M 04 Oct '22

04 Oct '22

Dear All If anyone can help Laura - see below, please contact her directly l.nicastro19(a)imperial.ac.uk<mailto:l.nicastro19@imperial.ac.uk> (Nicastro, Laura ) DO NOT REPLY TO THIS E_MAIL Bw Steve Hi Steve, I am looking for a small aliquot (3-5 ul) of the antibody Bcl-2. Would you mind circulating this email in the building to see if anyone has some to spare? Thank you very much in advance! Best, Laura

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[FILM-Users 01050] Fwd: Workshop ‘Automated Microscopy for Everyone’ by Acquifer - Automated Zebrafish Imaging - Please register for Demonstration and Hands-on Session
by Gaboriau, David C A 04 Oct '22

04 Oct '22

Dear FILM users, The Pantazis lab in Bioengineering is organising an instrument demo of the ACQUIFER Imaging Machine in Bessemer building at the end of October, please see below for details of the seminar and workshop. All the best, David Begin forwarded message: From: "Pantazis, Periklis L" <p.pantazis(a)imperial.ac.uk<mailto:p.pantazis@imperial.ac.uk>> Subject: Workshop ‘Automated Microscopy for Everyone’ by Acquifer - Automated Zebrafish Imaging - Please register for Demonstration and Hands-on Session Date: 1 October 2022 at 00:34:41 BST Dear London Zebrafish PI Community, dear London Zebrafish Club organising committee, I hope you are doing fine. The Pantazis Lab will be hosting a Workshop ‘Automated Microscopy for Everyone’ by Acquifer at the Department of Bioengineering of Imperial College London (Bessemer Building – see attached map). Acquifer will present the ACQUIFER Imaging Machine, an automated microscope following a unique opto-mechanical design rendering it ideal for imaging non-adherent, motion sensitive or large specimens. The Programme is as follows: * Online Seminar: 19th of October 2022, 14:00 to 16:00 * Demonstration and Hands-on Sessions: 25th to 27th of October, 2022 3-4 hour slots per small group (4 to 5 participants) It will be great if you can distribute the attached Acquifer Workshop pdf (includes a link for registration)among your lab members/the zebrafish community at your earliest convenience. I am happy to address any further questions! We will be excited to welcome many users at our place! Best wishes and take care, Laki __ Periklis (Laki) Pantazis PhD Imperial College London | Department of Bioengineering | South Kensington Campus | London SW7 2AZ Laboratory of Advanced Optical Precision Imaging | Lab B104 | Office RSM314 Tel: +44 (0)20 7594 6367 | Web: http://www.pantazis-lab.org<http://www.pantazis-lab.org/> | Email: p.pantazis(a)imperial.ac.uk <mailto:p.pantazis@imperial.ac.uk%C2%A0>

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