June 2021 - FILM-Users - imperial.ac.uk

[FILM-Users 00972] FW: Reminder - Cardio Respiratory Interface (CRI) Section External Seminar Series
by Bruckbauer, Andreas 24 Jun '21

24 Jun '21

FYI, Debora was FILM manager until 2017 Dr Debora Olivier (née Keller) Research Engineer of Telomeres and Genome Organization Lab, Centre de Biologie Intégrative, Université Toulouse III - Paul Sabatier "Assessing telomere nuclear organisation using 3D spatial statistics" Wednesday 30th June 2021 from 12:00 - 1:00 pm (Host: Dr Andreas Bruckbauer) Please Join us at the Cardio Respiratory Interface Section External Seminar Series. We will have an exciting talk from Dr Debora Olivier (née Keller) on "Assessing telomere nuclear organisation using 3D spatial statistics". Debora Olivier is a cell biologist by training with a strong interest in advanced microscopies. She is currently a research engineer in Laure Crabbé's lab (now at CBI Toulouse) and developed both imaging and analysis approaches to study telomere dynamics and spatial organisation. Please join by clicking on the link below: Join on Teams<https://teams.microsoft.com/l/meetup-join/19%3af2502db818d94b33803ec909ff1e…> To find out more details about Debora's talk, please click here<https://www.imperial.ac.uk/events/135680/assessing-telomere-nuclear-organis…>. Many thanks. Best regards, Sek Shir on behalf of the CRI section Sek Shir Cheong PhD Postdoctoral Research Associate Cario Respiratory Interface Section | National Heart and Lung Institute Faculty of Medicine | Imperial College London Level 2 Desk 10 Sir Alexander Fleming Building London SW7 2AZ www.imperial.ac.uk/people/sek-shir.cheong<http://www.imperial.ac.uk/people/sek-shir.cheong> www.lungdevelopmentandrepair.org/sek-shir-cheong/<https://www.lungdevelopmentandrepair.org/sek-shir-cheong/>

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[FILM-Users 00971] donkey serum
by Rothery, Stephen M 16 Jun '21

16 Jun '21

Dear All If anyone can help Helen with donkey serum please contact her direct - see below Please do not reply to this e-mail Thank-you Dear All, I'm currently in dire need of some donkey serum for some IHC staining. We've got three different orders from two suppliers open that just can't seem to come in. I would only need a small amount for now (probably around 1 ml) - obviously happy to replenish as soon as ours comes in. If anyone can help out I'd be really grateful. Stoelting, Helen <helen.stoelting17(a)imperial.ac.uk> Best wishes Helen Helen Stölting FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00970] !!!!!!!!!!! DATA DELETION SCRATCH2 DRIVE !!!!!!!!!!!!!!!!!!!!!!!!
by Rothery, Stephen M 16 Jun '21

16 Jun '21

Dear All The scratch2 drive is getting full, so we need to delete older files from it We will delete files older than the 1st October 2020 next week Please make sure you have everything backed up from before then Also you are reminded that the scratch drive is for transferring FILM data only It is not for long term storage or for users to store other data and programs If you are working offsite then scratch can be accessed either through VPN or using the remote gateway to logon to you work PC Many thanks Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00969] Sterile multiwell plate for suspension culture 48-well PS clear single packed
by Bruckbauer, Andreas 10 Jun '21

10 Jun '21

Can anyone help Silvia? Please see below and respond to her directly/ Best wishes Andreas From: Rodrigues Mendes Ferreira, Silvia A <s.ferreira(a)imperial.ac.uk> Sent: 10 June 2021 09:34 To: Bruckbauer, Andreas <a.bruckbauer(a)imperial.ac.uk> Subject: Question Hi Andreas, I need 2x sterile multiwell plate for suspension culture 48-well PS clear single packed. I ordered them and they will arrive end of June. Can you please ask if someone could give me 2 of those plates? I would return as soon as they arrive. Thank you so much in advance. Silvia A. Ferreira, PhD Inflammation, Repair and Development National Heart & Lung Institute Faculty of Medicine | Imperial College London Sir Alexander Fleming Building | South Kensington Campus London SW7 2AZ | United Kingdom Professor Sara Rankin Lab https://www.imperial.ac.uk/people/s.rankin PWP http://www.imperial.ac.uk/people/s.ferreira E-mail: s.ferreira(a)imperial.ac.uk<mailto:s.ferreira@imperial.ac.uk> Get Outlook for iOS<https://aka.ms/o0ukef>

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[FILM-Users 00968] Request for help - HEK293 cells
by Gaboriau, David C A 08 Jun '21

08 Jun '21

Dear Users, Talia Shmool is looking for someone who has HEK293 cells and could help doing some experiments looking at treating the cells with FITC-IgG-coated nanoparticles. Please see details below. If you can help, please contact Talia directly: t.shmool20(a)imperial.ac.uk All the best, David ---------------------------------------------------- David Gaboriau, PhD, FRMS FILM Microscopy Specialist Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 408 +44 (0)20 7594 9793 d.gaboriau(a)imperial.ac.uk https://www.imperial.ac.uk/people/d.gaboriau http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ --------------------------------------------------- ________________________________ From: Shmool, Talia A <t.shmool20(a)imperial.ac.uk> Sent: 04 June 2021 10:38 To: Gaboriau, David C A <d.gaboriau(a)imperial.ac.uk> Subject: Confocal microscopy Dear David, Thank you for taking the time to speak with me. I would like to examine the intracellular delivery of FTIC-IgG samples to HEK-293 cells by confocal microscopy. My samples are FTIC-IgG with and without nanoparticles in different formulations contains several excipient (I.e., sugar, surfactant, amino acids). I have 10 samples in total at the moment, likely will only need to examine 6. Please let me know if there is anyone who can help me out in conducting these experiments. Thanks, Talia Get Outlook for iOS<https://aka.ms/o0ukef>

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[FILM-Users 00967] autophagy inhibitor request
by Rothery, Stephen M 07 Jun '21

07 Jun '21

Dear microscopists, I am looking for an autophagy inhibitor to try and stabilise a protein I am working on. I would like to run a quick test and wonder if anyone could help me out with an aliquot. If you can spare some, please email to m.weinert13(a)ic.ac.uk<mailto:m.weinert13@ic.ac.uk> Please do not reply to this e-mail Many thanks! Maria FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00966] nicotinic acetylcholine receptors using alpha-bungarotoxin
by Rothery, Stephen M 03 Jun '21

03 Jun '21

Dear All If anyone can help Neda - see below - please send her an e-mail directly - Mohammadi, Neda <neda.mohammadi18(a)imperial.ac.uk> PLEASE DO NOT REPLY TO THIS E_MAIL Dear Steve, I would like to stain for nicotinic acetylcholine receptors using alpha-bungarotoxin, but I do not have this antibody. Would it be possible for you to share this request in case anybody is able to lend me some? Many thanks for your help, Neda FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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