Dear FILM-users,
A big thank you to the 70 people (18% of FILM users) who completed our survey back in February 2020! I realized that I never shared the results. I have attached the complete report, but want to highlight some results:
77% of our users rated the training as "extremely good" and 75% rated the facility overall as "extremely good", many thanks for your trust, we will continue to do our best.
The question if our microscopes are up to date was answered with 'Definitely yes' by 36% and 'Probably yes' by 50% while 14% did not think so.
Light-Sheet microscopy was very high on the list of new techniques and we have addressed this now with the new LS1 microscope at South Kensington and further grant applications for super-resolution microscopy and intra-vital imaging at Hammersmith are submitted.
To acquire new microscopes and replace the existing ones, grant applications are very important and we rely on PIs and departments to support these. Many thanks for your contributions!
Best wishes
Andreas
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Dr Andreas Bruckbauer
FILM facility manager
Sir Alexander Fleming Building
South Kensington Campus
Imperial College London
Exhibition Road
London, SW7 2AZ
SAF 4th floor, open space desk 401
+44 (0)20 7594 2023
a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk>
http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/
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Dear Microscopists,
We will keep our microscope charges constant during these challenging times, at least for now. They are calculated according to full economic costing guidelines to recover costs without making a profit. However due to a change in the way we pay for our MP laser maintenance, we can pass on cost savings and reduce the re-charges for MP laser usage from £18 to £10/hour. The staff time, which we would e.g. charge when we do imaging for users, increases from £29 to £37 as the Wellcome funding for David's position ended.
FILM will review the re-charges regularly during the year, so there might be future changes and I will keep you informed. You can always find the up-to date pricing on our Website:
https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…
best wishes
Andreas
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Dr Andreas Bruckbauer
FILM facility manager
Sir Alexander Fleming Building
South Kensington Campus
Imperial College London
Exhibition Road
London, SW7 2AZ
SAF 4th floor, open space desk 401
+44 (0)20 7594 2023
a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk>
http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/
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Can anyone help Lorraine? Please see below and reply to her directly l.lawrence(a)imperial.ac.uk<mailto:l.lawrence@imperial.ac.uk>
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Hello Everyone,
I have been asked to recommend a reliable make and model of cryostat. Does anyone have one they recommend ,and have personal experience of? Thanks.l.lawrence(a)imperial.ac.uk<mailto:Thanks.l.lawrence@imperial.ac.uk>
BW
Lorraine
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Dr Andreas Bruckbauer
FILM facility manager
Sir Alexander Fleming Building
South Kensington Campus
Imperial College London
Exhibition Road
London, SW7 2AZ
SAF 4th floor, open space desk 401
+44 (0)20 7594 2023
a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk>
http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/http://www.imperial.ac.uk/people/a.bruckbauer
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Dear Microscopists,
I am excited to announce that we now have a light-sheet microscope in our line-up of instruments in FILM!
Our new Leica Stellaris 5 DLS (Digital Light Sheet) microscope and was funded by the BBSRC 19 Alert call, proposal 'A digital light-sheet microscope for the analysis of biological processes under almost natural conditions' thanks to lead applicant Periklis Pantazis from Bioengineering. The microscope is located at South Kensington.
This microscope is ideal for volumetric imaging of medium sized samples and does this very gently with minimum phototoxicity. It allows 3D-imaging of e.g. zebrafish or spheroids/organoids over time. Furthermore photo-manipulation by confocal microscopy is possible using the same instrument. There are certain requirements for the sample mounting in light-sheet microscopy, which are different from our standard widefield and confocal systems, so please contact me if you are interested in using this new microscope so that we can discuss.
https://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscop…https://www.leica-microsystems.com/products/confocal-microscopes/p/stellari…
best wishes
Andreas
----------------------------------------------------
Dr Andreas Bruckbauer
FILM facility manager
Sir Alexander Fleming Building
South Kensington Campus
Imperial College London
Exhibition Road
London, SW7 2AZ
SAF 4th floor, open space desk 401
+44 (0)20 7594 2023
a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk>
http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/http://www.imperial.ac.uk/people/a.bruckbauer
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