December 2021 - FILM-Users

[FILM-Users 00992] Fridge in widefield microscope room 409
by Bruckbauer, Andreas 23 Feb '22

23 Feb '22

Dear FILM-users, We will get a new fridge in room 409 soon, if you still have samples in the old fridge, please remove them as soon as possible. In case you need to store them in the fridge for your experiment, they must be labelled with your name and date, so that we can transfer them safely to the new fridge. All unlabelled or old samples (older than 1 month) will be disposed of. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 01004] Glutaraldehyde solution for Tuesday/Wednesday?
by Bruckbauer, Andreas 21 Dec '21

21 Dec '21

Dear FILM-users, Can anybody spare 50ml of glutaraldehyde solution on Tuesday/Wednesday for Thomas Hall? Please contact him directly thomas.hall14(a)imperial.ac.uk<mailto:thomas.hall14@imperial.ac.uk> Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 01003] Please switch microscopes off
by Bruckbauer, Andreas 20 Dec '21

20 Dec '21

Dear FILM-users, If you do microscopy on the last day before the College closure (23/12) or during the closure days (if you have access), please switch the microscope off completely when you are finished, even when another person is booked as they might not show up. Access to College buildings will be restricted from 18:00 Thursday 23rd December 2021 to 07:00 Tuesday 4th January 2022. If you need be at work on Christmas Day or New Year's Day, contact your Head of Department, who should contact Security<mailto:id.card@imperial.ac.uk> for authorisation. The FILM team wishes you and your families a very Happy Christmas and New Year! Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 01002] centering telescope
by Bruckbauer, Andreas 16 Dec '21

16 Dec '21

Please contact Alexander Bailey directly if you can help a.bailey18(a)imperial.ac.uk<mailto:a.bailey18@imperial.ac.uk> ------ Would you be able to send an email round asking whether anyone has a centering telescope U-CT30 we could borrow for a short period (after the Christmas break) as we are setting up an Olympus microscope for phase contrast and need to set up some new objectives? Any help would be great! Thanks, and Best Wishes, Alex ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 01001] FW: Looking for isotype controls
by Bruckbauer, Andreas 08 Dec '21

08 Dec '21

If you can help Kornelija (see below), please contact her directly kornelija.suveizdyte15(a)imperial.ac.uk<mailto:kornelija.suveizdyte15@imperial.ac.uk> Andreas From: Suveizdyte, Kornelija <kornelija.suveizdyte15(a)imperial.ac.uk> Sent: 08 December 2021 13:03 To: Bruckbauer, Andreas <a.bruckbauer(a)imperial.ac.uk> Subject: Looking for isotype controls Hello Andreas, I am looking for a couple of isotype controls: a rat anti-mouse IgG2a and a rabbit anti-mouse IgG. Would you mind sharing this with FILM users in hope that someone has some and can spare me 2-3ul of each? Thank you, Kornelija Kornelija Suveizdytė PhD Student Inflammation, Repair and Development National Heart and Lung Institute Faculty of Medicine | Imperial College London Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ e-mail: kornelija.suveizdyte15(a)imperial.ac.uk<mailto:kornelija.suveizdyte15@imperial.ac.uk>

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[FILM-Users 01000] FW: ER antibody aliquot
by Rothery, Stephen M 06 Dec '21

06 Dec '21

Dear all Please message below If you are able to help please contact Zuzanna directly Zuzanna Jablonska, zuzanna.jablonska19(a)imperial.ac.uk<mailto:zuzanna.jablonska19@imperial.ac.uk> DO NOT REPLY TO THIS E-MAIL Many thanks Steve Hi Steve, I'm looking for a primary antibody against calnexin or calreticulin. Could you please send this out to the FILM users? Maybe I could get an aliquot from someone. Many thanks, Zuzanna

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[FILM-Users 00999] Ammonium Oxalate
by Bruckbauer, Andreas 01 Dec '21

01 Dec '21

Hello, Does anyone have a few grams of Ammonium Oxalate to spare for Lorraine Lawrence? Her supplier has let her down, please contact Lorraine directly l.lawrence(a)imperial.ac.uk<mailto:l.lawrence@imperial.ac.uk> Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00998] Chain Specific Secondary Antibodies
by Rothery, Stephen M 01 Dec '21

01 Dec '21

Dear all Please message below If you are able to help please contact Prag directly Pragati Pandey pragati.pandey13(a)imperial.ac.uk<mailto:pragati.pandey13@imperial.ac.uk> DO NOT REPLY TO THIS E-MAIL Many thanks Steve From: Pandey, Pragati <pragati.pandey13(a)imperial.ac.uk> Subject: Chain Specific Secondary Antibodies Hi Steve, I am looking for chain specific secondary antibodies for immunofluorescence ideally in red and far red. My primaries are raised in mouse and the isotypes are IgG1, IgG2a, IgG2b. Could you please circulate my request? Best Regards Prag Pragati Pandey National Heart and Lung Institute 4th Floor, ICTEM Building, Hammersmith Campus Imperial College London Du Cane Road London W12 0NN *: pragati.pandey13(a)imperial.ac.uk<mailto:pragati.pandey13@imperial.ac.uk> | *: 020 7594 2738

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