February 2019 - FILM-Users

[FILM-Users 00843] Microscope setup with two facing micropipette aspirators
by Bruckbauer, Andreas 11 Feb '19

11 Feb '19

Please see the email from Sam below and answer directly to him if you can help <s.au(a)imperial.ac.uk>, we don’t have this equipment in FILM. Bw Andreas From: Au, Sam Sent: 11 February 2019 16:23 To: Bruckbauer, Andreas <a.bruckbauer(a)imperial.ac.uk> Cc: Matellan, Carlos <c.matellan16(a)imperial.ac.uk> Subject: Re: Introduction Dear Andreas, Hope you are well, thank you again for touring me through FILM. One of my students, Carlos (cc'ed), needs a microscope setup with two facing micropipette aspirators for cell biophysics experiments. Do we happen to have such a setup in FILM, or do you happen to know if anyone has one on or off campus we could acccess? Many thanks. Best, Sam

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[FILM-Users 00842] Re: Grant application for Abbelight super-resolution microscope?
by Bruckbauer, Andreas 07 Feb '19

07 Feb '19

Dear FILM-users, We had the demo of a localization-based super-resolution microscope from Abbelight at King's last year and have been contacting people interested in this afterwards regarding a possible grant application. I just wanted see if there is additional interest which we might have missed. We are still looking for a committed lead-applicant with a matching research interest and can provide help writing the grant. This is about a microscope for single molecule based PALM/STORM super-resolution microscopy. It offers higher laser power, better resolution (20 nm x 20 nm x 35 nm), a larger field of view (compared to our existing Zeiss Elyra system) and better 3D capabilities. Please see the website for details https://www.abbelight.com/solutions/abbelight-instruments/#SAFe360 This instrument will be as demanding in sample preparation as our Elyra system and only works well with specific bright fluorophores, very high labelling density, special buffer conditions and it takes time to figure these out for an individual sample. Please contact me if you are interested in this project Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00841] Leica demo - Thunder-imaging-system March 5th and 6th
by Bruckbauer, Andreas 04 Feb '19

04 Feb '19

Dear all, Leica has a new imaging technology called Thunder: https://www.leica-microsystems.com/products/thunder-imaging-systems/ This is a widefield microscope capable of real-time deconvolution during image acquisition. They are keen to test this on real samples, and are willing to bring in a system on the 5th and 6th of March for a demo. Please contact me if you have suitable samples to be tested, this could be cells or tissue sections etc. to be imaged in 3D, see the website for inspiration. The Thunder technology should remove the out of focus blur as we do currently with the Huygens software, but you would see it directly when acquiring the images. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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