December 2018 - FILM-Users

[FILM-Users 00837] Postdoc vacancy in super-res microscopy of immune cells - Manchester
by Bruckbauer, Andreas 19 Dec '18

19 Dec '18

fyi: Hi, We have a new postdoc position available in super-resolution microscopy of immune cell biology. Please circulate to anyone who may be interested! Thanks. Details: https://www.jobs.manchester.ac.uk/displayjob.aspx?jobid=16551 Daniel M. Davis Professor of Immunology Director of Research Manchester Collaborative Center for Inflammation Research University of Manchester CTF Building 46 Grafton Street Manchester M13 9NT Tel: 0161 275 5019

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[FILM-Users 00830] !!! Changes to usage regulations
by Bruckbauer, Andreas 19 Dec '18

19 Dec '18

Dear FILM-users, We had to make a few changes to our usage regulations, you will be presented to the new documents when you log into PPMS, the main changes to the regulations are: In order to prevent Masters and BSc students to book microscopes outside 07:00 - 19:00 , we had to change the peak hours, which are now from 07:00 - 19:00. An update on the lone working policy is also included. Due to high demand on the confocal microscopes, we have to restrict the maximum hours which can be booked per day: Widefield microscopes: Booking during peak hours (7-19h) is restricted to a maximum of 12h/day; there is a weekly booking limit of 16h during peak time per user, so as to allow others to gain access to the instrument. The booking restriction is lifted if the microscope is not booked 24h beforehand. Confocal microscopes: Booking during peak hours (7-19h) is restricted to a maximum of 6h/day; there is a weekly booking limit of 12h during peak time per user, so as to allow others to gain access to the instrument. The booking restriction is lifted if the microscope is not booked 24h beforehand. Please contact facility staff if you need to book for longer times. I also updated the health & safety document with the lone working regulations. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00835] FW: access to College buildings during the Christmas closure
by Bruckbauer, Andreas 11 Dec '18

11 Dec '18

Dear all, I hope you don't need to work in the FILM rooms on College Closure Days, otherwise you need written approval by your Head of Department, for NHLI see below. Best wishes Andreas From: Hipwell, Mandy A Sent: 10 December 2018 10:38 Subject: FW: access to College buildings during the Christmas closure FYI From: Hyde, Gareth D Sent: 07 December 2018 11:00 Subject: access to College buildings during the Christmas closure Dear All, As you all know the Provosts board this year introduced a policy that requires access to College buildings between midnight and 7am or at any time during College closure periods to be approved by the Head of Department (Prof Edwin Chilvers). See the attached for further information and a link to the application form. If any staff in your areas require access to carry out work during the College Christmas closure please ensure that they complete an application and send it to me by Friday the 14th of December at the latest. This is to ensure there is time for Prof Chilvers to sign the applications and for them to be processed by Security. Please remember to clearly indicate in the application why this work must happen during a College closure period. Applications will not be approved for emergency access only. In the case of emergencies e.g. freezer failures, Security should be contacted on 020 7589 1000 and they will be able to let you into College buildings. Best wishes Gareth Safety and Technical Services Manager NHLI Imperial College

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[FILM-Users 00834] help with beta-galactosidase assays
by Bruckbauer, Andreas 04 Dec '18

04 Dec '18

Is someone able to help Robyn? Please contact them directly: I have transfected suspension HEK cells with a positive control plasmid containing the LacZ reporter gene. I wondered if you knew of any resources available to stain the cells with X-gal or anyone performing beta-galactosidase assays that may be willing for me to try it out on my small number of samples prior to investing in my own reagents? Any help would be greatly appreciated! Many thanks, Robyn Bell, Robyn <robyn.bell15(a)imperial.ac.uk> ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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