November 2018 - FILM-Users

[FILM-Users 00830] !!! Changes to usage regulations
by Bruckbauer, Andreas 19 Dec '18

19 Dec '18

Dear FILM-users, We had to make a few changes to our usage regulations, you will be presented to the new documents when you log into PPMS, the main changes to the regulations are: In order to prevent Masters and BSc students to book microscopes outside 07:00 - 19:00 , we had to change the peak hours, which are now from 07:00 - 19:00. An update on the lone working policy is also included. Due to high demand on the confocal microscopes, we have to restrict the maximum hours which can be booked per day: Widefield microscopes: Booking during peak hours (7-19h) is restricted to a maximum of 12h/day; there is a weekly booking limit of 16h during peak time per user, so as to allow others to gain access to the instrument. The booking restriction is lifted if the microscope is not booked 24h beforehand. Confocal microscopes: Booking during peak hours (7-19h) is restricted to a maximum of 6h/day; there is a weekly booking limit of 12h during peak time per user, so as to allow others to gain access to the instrument. The booking restriction is lifted if the microscope is not booked 24h beforehand. Please contact facility staff if you need to book for longer times. I also updated the health & safety document with the lone working regulations. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00833] FW: Image Contest
by Bruckbauer, Andreas 21 Nov '18

21 Nov '18

Please see below: From: Thwaites, Ryan S Sent: 20 November 2018 12:11 To: FILM Service <film-service(a)imperial.ac.uk> Subject: Image Contest Dear FILM team, I am a post-doc in the NHLI and represent the Society for Mucosal Immunology (SMI) Communication committee. The SMI are currently running an image competition that I was wondering if you could advertise? The first place prize is $1500 towards attendance of the SMI 2019 congress in Brisbane, Australia. I expect that many of your facility users might be interested. If you are interested the details and entry for the competition can be found here: http://www.socmucimm.org/icmi-2019/contest/ , the deadline for entry is the 15th December 2018. Best wishes, Ryan Thwaites Research Associate Imperial Clinical Respiratory Research Unit (ICRRU) 1st Floor, Mint Wing St Marys Hospital Imperial College London W2 1NY

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[FILM-Users 00832] Sample for fluorescence microscopy
by Bruckbauer, Andreas 21 Nov '18

21 Nov '18

Dear all, One of our users, Abderrahim Boualam, abderrahim.boualam16(a)imperial.ac.uk<mailto:abderrahim.boualam16@imperial.ac.uk> from bioengineering is asking if anyone has a spare (biological) sample for fluorescence microscopy with low fluorophore concentration which bleaches easily? Please contact him directly if you can help. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00831] FLIM-FRET seminar - Sean Warren- on Monday 12th November from 1-2pm
by Bruckbauer, Andreas 09 Nov '18

09 Nov '18

Sean Warren, who is a former member of the Photonics group in Physics and who wrote the FLIMfit software package for analysing fluorescence lifetime image data, will be giving a seminar on Monday 12th November from 1-2pm in Blackett room B636: Dr. Sean Warren Garvan Institute of Medical Research In vivo quantification of FRET biosensors using multispectral FLIM-FRET Intravital imaging of genetically expressed Förster resonance energy transfer (FRET) biosensors can provide unique insights into the function of biological processes in a native context. However, quantitative analysis of intravital FRET data is often compromised by motion, tissue or drug autofluorescence and scattering. We demonstrate tools to overcome these challenges and so reliably obtain accurate FRET quantification in vivo. We first demonstrate a new open source software tool, Galene, for image-based correction of sample motion blurring in both time resolved and conventional laser scanning fluorescence microscopy data in two and three dimensions. We show that we can resolve intravital FRET images of intra-abdominal organs in a murine model. We then introduce a multispectral fluorescence lifetime imaging (FLIM)-FRET analysis approach which allows reliable quantification of FRET biosensors in a variety of intravital contexts. We demonstrate that we can measure FRET activity in the presence of high levels of autofluorescence from both tissue and administered small molecule drugs. Finally, we demonstrate that this multispectral approach can be used to image two highly overlapping FRET biosensors using a genetically engineered mouse model expressing FRET biosensors for the prototypical RhoGTPases RhoA and Rac1. This approach allows us to directly visualise the mutually inhibitory response of RhoA and Rac1 on a subcellular level to treatment with small molecule inhibitors and activators. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ http://www.imperial.ac.uk/people/a.bruckbauer ----------------------------------------------------

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[FILM-Users 00829] Reminder for data saving
by Gaboriau, David C A 06 Nov '18

06 Nov '18

Dear all, Just a quick reminder to save your microscopy data on the local hard drive of the microscope you are using, and then copy all the data to your folder on the new network drive Scratch2 (Scratch has been discontinued). You can map Scratch2 at the following address: \\film-nas2.med.ic.ac.uk\scratch2<file:///\\film-nas2.med.ic.ac.uk\scratch2> All the best and Happy Imaging! David David Gaboriau, PhD FILM - Facility for Imaging by Light Microscopy High-Content Microscopy Specialist Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)2075 949 793 Email: d.gaboriau(a)imperial.ac.uk<mailto:d.gaboriau@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility Page: http://www.imperial.ac.uk/people/d.gaboriau

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