June 2017 - FILM-Users - imperial.ac.uk

[FILM-Users 00752] HCF1 Hammersmith Confocal
by Rothery, Stephen M 28 Jun '17

28 Jun '17

Re. HCF1 Hammersmith Confocal Dear All The re-install is complete and all is working The system had to be completely wiped so no images or settings were retained This means that each user will have to setup their profile. Easy Instructions are by the microscope. Also if you want to re-use an old image then copy it to the scratch drive and access it from the confocal !! IMPORTANT !! please do not download and install any software onto our machines as this can lead to problems Any problems please contact me on my mobile Number by the microscope Bw Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00751] FW: MDFI workshop July 21 - provisional programme
by Bruckbauer, Andreas 28 Jun '17

28 Jun '17

FYI: one day workshop on multidimensional fluorescence imaging on July 21st organised by the Photonics group Please see attached program ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00750] Hammersmith HCF1 Confocal
by Rothery, Stephen M 26 Jun '17

26 Jun '17

Re Hammersmith HCF1 Confocal The reload is almost completed but unfortunately the system will not be available until Wednesday to allow for all the windows updates to load and to have the system put back on the college network Apologies for the inconvenience Bw Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00749] ICR image analysist
by Black, Lucas 26 Jun '17

26 Jun '17

Dear All, This may be a little spammy, for which I apologise. The ICR are looking for an image analysist to work in the Imaging and Flow core. Details below. https://icr.tal.net/vx/lang-en-GB/mobile-0/appcentre-1/brand-4/candidate/so… Best, Lucas

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[FILM-Users 00748] Widefield microscopes Data deletion
by Rothery, Stephen M 23 Jun '17

23 Jun '17

Dear All The data drives on all the widefields (WF3 and HWF1) are getting full We need to delete older data We will be deleting the data saved up to the 1st March 2017 next week Please make sure you have your data backed up Bw Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00747] Hammersmith Confocal
by Rothery, Stephen M 23 Jun '17

23 Jun '17

Dear All users of the Hammersmith Confocal Next Monday (26th) we are having to reload windows. This might also mean the Data drive D will also be deleted. If you have not backed up your data please do so Also this will mean everyone's profile will need to be re-set. This is not a great problem and each user should be able to do this themselves. I will leave simple but explicit instructions on what to do - it should take no more than 2 minutes Bw Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00746] test sample for fluorescence microscopy
by Bruckbauer, Andreas 21 Jun '17

21 Jun '17

Dear all, Khushi Vyas from Engineering, is looking for a test sample for fluorescence microscopy, ideally a tissue sample which is fluorescent with 488 and 640 nm excitation. This would be to test her home built confocal microscope. Please contact her directly if you could provide any suitable samples. Vyas, Khushi <k.vyas13(a)imperial.ac.uk> Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00743] Damage to the 20x Objective
by Rothery, Stephen M 21 Jun '17

21 Jun '17

Dear All We have just discovered that the 20x objective on the Hammersmith widefield microscope is damaged and will not be available until it is replaced. This damage is likely caused when the stage is moved after imaging dishes and multiwall plates. These dishes and plates have a higher focus than slides which means the objectives can come into contact with the stage and holders when moving in X-Y. If you are using these sample holders please be extra careful and lower the focus after you have finished and return the turret to the 5x position. You are also reminded that objectives above x10 cannot reach the outer rows of 96 mutilwell plates without hitting the stage If you are aware that this has happened please let us know immediately so that we can sort it These objectives are costly to replace, also there is the inconvenience of downtime while we await the replacement. Please take care of all our microscopes and objectives Bw Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00744] FILM microscopy day 2017
by Bruckbauer, Andreas 20 Jun '17

20 Jun '17

Dear all, Only one week to go! Our microscopy day will be held Tuesday next week, please see the program below. You will be able to register on Evenbright https://www.eventbrite.co.uk/e/film-microscopy-day-2017-tickets-35537018178 While registration is not strictly necessary to attend the lectures, it helps us with ordering catering. Hope to see many you! Andreas FILM Microscopy Day 2017 Tuesday June 27th, 9.30-17.30 SAFB-G34 (Sir Alexander Fleming Building), South Kensington Campus 9:30 - 9:45 Tony Magee: Welcome & latest FILM news 9:45 - 10:30 Andreas Bruckbauer: From microscopy basics to state of the art applications. 10:30 - 11:00 Stephen Rothery: Introduction to Fiji and macro writing for image analysis. 11:00 - 11:30 Coffee & tea break 11:30 - 12:00 Debora Keller: PPMS, our new online booking system. 12:00 - 12:30 Microscopy Match-Maker: FILM users present their research performed in FILM Rebecca Rickman: Microscopy in Measuring Neuronal Connectivity. Magdalena Plotczyk: The use of microscopy to identify the properties of healthy and scarred dermis. Edwin A Garcia Castano: Wide-field fluorescence Life time Imaging in 3D multicellular spheroids expressing and AMPK FRET sensor. 12:30 - 13:30 Sandwich lunch 13:30 - 14:15 David Gaboriau: High Content Imaging. 14:15 - 14:45 Technobytes - 10min presentation from our sponsors: Paul McCormick, Leica microsystems, Full Spectral Freedom for Multi-Colour, Deep Imaging experiments with SP8 DIVE. Gareth Bolitho, Stratech Scientific Ltd, New and Bespoke Two Photon Probes. Olympus, TBC. 14:45 - 15:30 Cristina Lo Celso, Imperial College London: Imaging healthy and malignant haematopoiesis: active cells in a dynamic environment. 15.30 - 16.00 Coffee & tea break 16:00 - 16:55 Plenary Lecture Michael Way, The Francis Crick Institute: Using Vaccinia virus to understand signalling and cytoskeletal dynamics. 16:55 - 17:00 Tony Magee: Round-up. 17:00 - 17:30 The plenary lecture will be followed by drinks and nibbles Sponsored by: Stratech, Hamamatsu, Zeiss and maybe others Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00742] Debbi's leaving drinks - after FILM day on 27/06, 5pm
by Keller, Debora 15 Jun '17

15 Jun '17

Dear all, As some of you know (or most of you?), I have resigned from my post as facility manager and will be leaving FILM at the end of june. As a matter of fact, I will also be leaving the UK for Paris, back to my french roots 😊 I really had a great time at FILM, and enjoyed very much discussing with you, training you, helping you with your microscopy, and in general meeting great people! I will be having my leaving drinks after Microscopy Day, on 27th of june at 5pm until 6pm (ish). I do hope you have all registered for the Microscopy Match Maker and FILM day, and hope to see you then! If not, I will still be in the imaging community, but in Paris! and we know how small the scientific world is, so chances are, we might bump into each other at some point! My email address is deborakeller0(a)gmail.com - feel free to contact me if visiting in Paris! Thank you all for an amazing time! Cheers! Debbi Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<https://exchange.imperial.ac.uk/owa/redir.aspx?C=ly2HSraUakenznQlUce8xI9f3N…> Website: http://imperial.ac.uk/imagingfacility<https://exchange.imperial.ac.uk/owa/redir.aspx?C=ly2HSraUakenznQlUce8xI9f3N…>

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