February 2017 - FILM-Users

[FILM-Users 00711] Innovation Mixers - *Reminder* - 1st March 2017 - Next Generation Optical Microscopy Initiative: Imaging cellular structure and function beyond the diffraction limit
by Figueira, Helen 22 Feb '17

22 Feb '17

Next Generation Optical Microscopy Initiative: Imaging cellular structure and function beyond the diffraction limit Super-resolved microscopy has revolutionised optical imaging by breaking the "diffraction limit" of optical resolution and achieving a resolution down to 10's of nm. Life Sciences researchers with interest in fields such as epigenetics, gene regulation and metabolism can now visualise biological structures and functions on the molecular scale in live cells and organisms. Teams at MRC LMS and Imperial College London have recently partnered to develop instruments, capabilities and imaging techniques such as SIM, STED, 3-D PALM STORM and FLIM-FRET to visualise and study biological structures and processes. In the innovation mixer on Wednesday, 1st March at 2.30pm we will highlight our advances in the field and present some in-house developments of these novel techniques.

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[FILM-Users 00710] !!!!!!!!!!!!!!!!!!!! Data Deletion Definiens/Huygens drive !!!!!!!!!!!!!!!!!!!!!
by Rothery, Stephen M 16 Feb '17

16 Feb '17

Dear users of Huygens and Definiens The data drive (Definiens) is getting full again and we need to delete older data We will be deleting data from before the 1st July 2016 on Monday Please make sure your data is backed up Bw Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00709] FW: Postdoctoral fellowships in Berlin
by Bruckbauer, Andreas 14 Feb '17

14 Feb '17

FYI, this should be of interest to cutting edge microscopists: From: Helge Ewers [mailto:helge.ewers@fu-berlin.de] Sent: 14 February 2017 07:11 To: helge.ewers(a)fu-berlin.de<mailto:helge.ewers@fu-berlin.de> Subject: Postdoctoral fellowships in Berlin Dear all, there are postdoctoral Fellowships available at the FU Berlin: http://www.fu-berlin.de/en/sites/promovieren/drs/drs_fellowships/DAHLEM-POS… if you know someone interested to move to Berlin with an interest in Biophysics or the like, my lab is found under “nanoscale”. Other exciting stuff is available there, too! Thanks for forwarding to interested “senior PhD-students" Best wishes, Helge -- Dr. Helge Ewers Professor for Membrane Biochemistry Freie Universität Berlin Institute for Chemistry and Biochemistry Thielallee 63 14195 Berlin Germany Email: helge.ewers(a)fu-berlin.de<mailto:helge.ewers@fu-berlin.de> Telephone: +49 30 838 60644

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[FILM-Users 00708] STORM workshop at Hammersmith
by Bruckbauer, Andreas 13 Feb '17

13 Feb '17

Dear all, If you like to attend the STORM workshop organised by Photonics on March 1st, please contact Helen Figueira, helen.figueira(a)lms.mrc.ac.uk<mailto:helen.figueira@lms.mrc.ac.uk> the workshop will be in the CSC seminar room, level 2 the Commonwealth Building on the Hammersmith Campus. Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ---------------------------------------------------- From: Bruckbauer, Andreas Sent: 06 February 2017 10:31 To: film-users <film-users(a)imperial.ac.uk> Cc: Nikolopoulou, Lina <lina.nikolopoulou13(a)imperial.ac.uk>; 'Ashdown, George' <george.ashdown(a)kcl.ac.uk>; Tahirbegi, Bogachan <b.tahirbegi(a)imperial.ac.uk>; Gaffney, Piers R J <p.gaffney(a)imperial.ac.uk> Subject: STORM workshop at Some info for all current and potential super-resolution users: Paul French's group organises an informal workshop at Hammersmith, the aim is to tell biologists about the instruments they have developed and to encourage them to work with them to apply super-resolution microscopy to their questions, please join us! - Andreas Next Generation Optical Microscopy Initiative: Imaging cellular structure and function beyond the diffraction limit Wednesday 1st March, MRC London Institute of Medical Sciences (LMS) seminar room 1430 Matthias Merkenschlager & Paul French Introduction & overview 1450 SRM & FLIM capabilities Kwasi Kwakwa - EasySTORM Ian Munro - STORM data analysis on HPC Stina Guldbrand - STED with NP Frederik Görlitz - SIM +FLIM Dirk Dorman - CSC SRM 1540 CSC aspirations & progress Sheila Xie - STORM of AID Angel L. Jaso Tamame - SRM to study meiosis Anne-Celine Kohler - Label-free STORM of DNA Alex Sardini - FLIM of AMPK FRET sensors in 2D &3D cell culture 1620 Tea 1640 Application of SRM Robert Mahen (Imperial/LMB) Super-resolved microscopy of centrosome dynamics 1700 Application of SRM Dylan Owen (KCL) - "Statistical analysis of molecular distributions derived from PALM and STORM data" 1730 Discussion & drinks Future work - probes & priorities ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00707] FW: Art of Research - call for images
by Magee, Tony 08 Feb '17

08 Feb '17

From: Johnson, Helen L Sent: 08 February 2017 10:47 Subject: Art of Research - call for images Scientific imagery competition now open [mperial College London] View online<https://wwwf.imperial.ac.uk/utils/apps/mail/web/189721096/art-of-reseach---…> Scientific Imagery Competition Art of Research: call for images Following last year’s successful competition, Imperial Innovations have launched this year's call for great scientific imagery from across Imperial College London. As before, both staff and students are invited to submit images that reflect or illustrate their scientific research or work. This year, we are also accepting short video clips! Full details of the competition are on the webpage<http://www.imperialinnovations.co.uk/technology-transfer/art-research-scien…>. We will be accepting submissions until the 26th February 2017. [https://www.imperial.ac.uk/ImageCropToolT4/imageTool/uploaded-images/Digita…]<http://www.imperialinnovations.co.uk/technology-transfer/art-research-scien…> [acebook]<https://www.facebook.com/imperialcollegelondon> [witter]<https://twitter.com/imperialcollege> [ouTube]<https://www.youtube.com/user/imperialcollegevideo> [inkedIn]<https://www.linkedin.com/edu/imperial-college-london-12598> [oogle Plus]<https://plus.google.com/100357106915062883581/posts> [nstagram]<https://instagram.com/imperialcollege/> Imperial College London<https://www.imperial.ac.uk?utm_medium=email&utm_campaign=Art+of+Reseach+-+c…> • South Kensington Campus, London, SW7 2AZ • +44 (0)20 7589 5111

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[FILM-Users 00706] Questions: OMERO data sharing platform
by Bruckbauer, Andreas 06 Feb '17

06 Feb '17

Dear all, Together with the bioinformatics data science group FILM has offered the data-sharing platform OMERO http://www.imperial.ac.uk/bioinformatics-data-science-group/resources/softw… This is a great tool to store, access and share microscopy data with people inside and outside of the College. However it requires significant staff time to be maintained. It would be great if users, especially PIs, would answer the following questions: - Are you currently using OMERO? - Are you planning to use OMERO in the future? - Could OMERO help you to fulfil data sharing obligations by funding bodies? Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00705] !!!!!!!!!!!!!!!!!!!!!!!!!!!! DELETION OF SCRATCH !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
by Rothery, Stephen M 06 Feb '17

06 Feb '17

Dear All the scratch disk is getting full again so we need to clean it up we will be deleting all files dated from before 1st September 2016 on Friday please make sure you have backed up your data you are reminded that scratch is for transferring data only - not for long term storage or storing your own data many thanks Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00704] STORM workshop at
by Bruckbauer, Andreas 06 Feb '17

06 Feb '17

Some info for all current and potential super-resolution users: Paul French's group organises an informal workshop at Hammersmith, the aim is to tell biologists about the instruments they have developed and to encourage them to work with them to apply super-resolution microscopy to their questions, please join us! - Andreas Next Generation Optical Microscopy Initiative: Imaging cellular structure and function beyond the diffraction limit Wednesday 1st March, MRC London Institute of Medical Sciences (LMS) seminar room 1430 Matthias Merkenschlager & Paul French Introduction & overview 1450 SRM & FLIM capabilities Kwasi Kwakwa - EasySTORM Ian Munro - STORM data analysis on HPC Stina Guldbrand - STED with NP Frederik Görlitz - SIM +FLIM Dirk Dorman - CSC SRM 1540 CSC aspirations & progress Sheila Xie - STORM of AID Angel L. Jaso Tamame - SRM to study meiosis Anne-Celine Kohler - Label-free STORM of DNA Alex Sardini - FLIM of AMPK FRET sensors in 2D &3D cell culture 1620 Tea 1640 Application of SRM Robert Mahen (Imperial/LMB) Super-resolved microscopy of centrosome dynamics 1700 Application of SRM Dylan Owen (KCL) - "Statistical analysis of molecular distributions derived from PALM and STORM data" 1730 Discussion & drinks Future work - probes & priorities ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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