January 2017 - FILM-Users - imperial.ac.uk

[FILM-Users 00703] Filters widefield 2
by Rothery, Stephen M 31 Jan '17

31 Jan '17

If the person who has borrowed the Dapi and Quad filters from the widefield 2 microscope please return them immediately This is both annoying and against our rules Bw steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ **************************************************************

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[FILM-Users 00702] dSTORM sample for 3D imaging
by Bruckbauer, Andreas 24 Jan '17

24 Jan '17

Sorry for the SPAM, I got this question from Ben Owen from Oxford Nanoimaging Ltd: I wondered if you could help us? We need a dSTORM sample to show a customer our 3D developments but we don't have any suitable biological samples. Would you know someone in Imperial that could help? We ideally would like a 2 colour sample but 1 colour would be fine. Something like the cytoskeleton that has 3D structure would be great! The visit is on the 14 February. I can collect whenever is suitable. Could you put me in touch with someone with samples such as this? Any assistance would be most appreciated. I can't pay you but will help in any suitable way! Thanks Ben If you want to help, please contact: Ben Owen ben.owen(a)oxfordni.com<mailto:ben.owen@oxfordni.com> http://www.oxfordni.com/ Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00701] talk by Zeiss on 19/01 Airyscan, EM and X-ray microscopy
by Bruckbauer, Andreas 16 Jan '17

16 Jan '17

Dear FILM user, As part of the Zeiss Airyscan demo, we are hosting a talk by Ben Ewins and Dean Edwards from Zeiss about latest development in confocal microscopy (including the fast Airyscan) as well as EM and X-Ray microscopy. When: Thursday 19/01/2017 12:00 -13:00 Where: Meeting area level 6 at SAF Best wishes Andreas ---------------------------------------------------- Dr Andreas Bruckbauer FILM facility manager Sir Alexander Fleming Building South Kensington Campus Imperial College London Exhibition Road London, SW7 2AZ SAF 4th floor, open space desk 401 +44 (0)20 7594 2023 a.bruckbauer(a)imperial.ac.uk<mailto:a.bruckbauer@imperial.ac.uk> http://www.imperial.ac.uk/medicine/facility-for-imaging-by-light-microscopy/ ----------------------------------------------------

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[FILM-Users 00700] FW: Nikon job opportunity
by Gaboriau, David C A 11 Jan '17

11 Jan '17

Dear Users, Please see below the advert for an Advanced Imaging Specialist position with Nikon. All the best, David From: lsr-group-bounces(a)imperial.ac.uk [mailto:lsr-group-bounces@imperial.ac.uk] On Behalf Of Daniel.Metcalf(a)nikon.com Sent: 06 January 2017 12:02 To: lsr-group <lsr-group(a)imperial.ac.uk> Subject: [lsr-group] Nikon job opportunity Hi All, If you haven't already picked up this job advert elsewhere we are currently recruiting for a new Advanced Imaging Specialist (AIS): https://www.nikoninstruments.com/en_GB/Country-Specific/GB/About/Career-Opp… Our AIS for London who we recruited from Imperial last year forwarded the advert through this group so hopefully a few of you reading this may apply. Please forward onto your departments etc if possible. The area covered will be primarily Cambridge and Oxford so the person would need to be able to drive and get to both of those cities easily. It could suit someone living in, or willing to relocate to, North London (easy access to M25) or up the M1 corridor. Let me know if you have any questions or would like any more information. Best Regards, Dan [Image removed by sender.] Dan Metcalf Advanced Imaging Manager Instruments Nikon UK Limited Nikon House, 380 Richmond Road, Kingston, Surrey, KT2 5PR Mob: +44 (0) 7836 502 538 http://www.nikoninstruments.com/ This E-mail is strictly confidential and intended solely for persons or organisations to whom it is addressed.If you are not the intended recipient,you must not copy, distribute or take any action in reliance upon it. If you receive this E-mail in error, please notify the IT department of Nikon U.K. Limited (02084816861) as soon as possible, delete this E-mail from your computer and destroy any printed copies. Nikon U.K. Limited cannot accept any responsibility for the accuracy or completeness or security of this message as it has been transmitted over the Internet and has been relayed by a number of servers. If you suspect that the message has been altered, please inform us. At Nikon we operate by best practice and exercise an 'opt-out' policy. If you do not wish to be e-mailed in future by Nikon U.K. Limited please indicate this fact in your response. Nikon is the trading name of Nikon U.K. Limited which is a company registered in England and Wales with company number 1448987 and VAT registration number GB 243-0236-06 whose registered office is at 380 Richmond Road,Kingston,Surrey,KT2 5PR,England.

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[FILM-Users 00699] Fast microfluidic temperature control for high resolution live cell imaging
by Bruckbauer, Andreas 11 Jan '17

11 Jan '17

FYI, from Alex Cerise http://www.cherrybiotech.com/ In collaboration with the Institut Curie and the ENS Paris, we developed a system allowing an 0.1°C precise and stable temperature control on a large 5 to 45°C range. Publication : Guilhem Velve Casquillas et al. Lab Chip, 2011, 11, 484-489 Abstract: One major advantage of using genetically tractable model organisms such as the fission yeast Schizosaccharomyces pombe is the ability to construct temperature-sensitive mutations in a gene. The resulting gene product or protein behaves as wildtype at permissive temperatures. At non-permissive or restrictive temperatures the protein becomes unstable and some or all of its functions are abrogated. The protein regains its function when returning to a permissive temperature. In principle, temperature sensitive mutation enables precise temporal control of protein activity when coupled to a fast temperature controller. Current commercial temperature control devices do not have fast switching capability over a wide range of temperatures, making repeated temperature changes impossible or impractical at the cellular timescale of seconds or minutes. Microfabrication using soft-lithography is emerging as a powerful tool for cell biological research. We present here a simple disposable polydimethylsiloxane (PDMS) based microfluidic device capable of reversibly switching between 5 _C and 45 _C in less than 10 s. This device allows high-resolution live cell imaging with an oil immersion objective lens. We demonstrate the utility of this device for studying microtubule dynamics throughout the cell cycle.

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