March 2015 - FILM-Users - imperial.ac.uk

[FILM-Users 00551] FW: Jurkat cells needed
by Keller, Debora 26 Mar '15

26 Mar '15

If someone can help Cris, please contact him directly! Cheers Debbi ________________________________ From: Silvestri, Cristoforo Sent: 26 March 2015 14:09 To: Keller, Debora Subject: Jurkat cells Hello I am a post doc in the lab of Darryl Overby in Bioengineering. I am looking to obtain Jurkat cells and an internet search indicated that someone at least at FILM routinely uses Jurkat cells. Would it be possible for you to put me in contact with anyone that indeed has these cells? Thank you in advance for your help. Cris Department of Bioengineering, Room B313 Imperial College London, Exhibition Road, London SW7 2AZ

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[FILM-Users 00550] FYI Super-resolution workshop
by Keller, Debora 18 Mar '15

18 Mar '15

Dear FILM-users, There is a super-resolution workshop in France (see flyer attached) - with lectures and hands-on sessions. Deadline 27th March 2015 http://ateliersinserm.dakini.fr/en/accueil-66-12.php Could be good for students and postdocs wishing to do some super-res imaging down the line. cheers Debbi Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00549] FW: Image analysis for Life Science - deadline Tuesday 17th March
by Keller, Debora 16 Mar '15

16 Mar '15

Dear users, See below - this survey is meant to assess the needs in image analysis of the scientific community. It literally takes 20 seconds to fill - I just did it! :) Deadline Tuesday 17th March, 12pm. Cheers Debbi From: UK Euro-BioImaging discussion list [mailto:UK-EUROBIOIMAGING-PROJECT@JISCMAIL.AC.UK] On Behalf Of Lucy Collinson Sent: 13 March 2015 14:58 To: UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK<mailto:UK-EUROBIOIMAGING-PROJECT@JISCMAIL.AC.UK> Subject: Image analysis for Life Science - deadline Tuesday 17th March Importance: High Hi everyone In the view of a network initiative which would have a potential beneficial impact on the scientific community, we are trying to collect basic indicators about Image Analysis in the Life Sciences in Europe, to assess the current shortcomings. The survey will take 20 seconds, and your answers will greatly help us in depicting the state-of-the-art of our Life Science community when facing the needs and challenges of daily Image Analysis. You can find the form here... https://docs.google.com/forms/d/18IeFH5uSJrZoFvP9As9JyL4gSHPNqelpK6F1cFkKTW… Of course there could be many more questions, but the goal is to be concise and have it filled in by a maximum number of people. Please forward this on to your colleagues, departments and institutes as appropriate, but do note the short deadline (next Tuesday). We will provide statistics to anyone who is interested, you can opt to enter your email address which will only be used to send you the results of the survey. Thanks a lot in advance, Lucy (on behalf of image analysis community startup members). -- Lucy Collinson PhD Head of Electron Microscopy London Research Institute Cancer Research UK 44 Lincoln's Inn Fields London WC2A 3LY Tel: 020 7269 3416 Fax: 020 7269 2805 [Description: Description: cid:image004.png@01CD97DD.036ACD30] NOTICE AND DISCLAIMER This e-mail (including any attachments) is intended for the above-named person(s). If you are not the intended recipient, notify the sender immediately, delete this email from your system and do not disclose or use for any purpose. We may monitor all incoming and outgoing emails in line with current legislation. We have taken steps to ensure that this email and attachments are free from any virus, but it remains your responsibility to ensure that viruses do not adversely affect you. Cancer Research UK Registered charity in England and Wales (1089464), Scotland (SC041666) and the Isle of Man (1103) A company limited by guarantee. Registered company in England and Wales (4325234) and the Isle of Man (5713F). Registered Office Address: Angel Building, 407 St John Street, London EC1V 4AD. -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK<mailto:UK-EUROBIOIMAGING-PROJECT@JISCMAIL.AC.UK> To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT --

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[FILM-Users 00548] Electrical testing at FILM THIS WEEKEND
by Keller, Debora 12 Mar '15

12 Mar '15

Dear users, This WE it is the turn of the FILM microscopy rooms on the 4th floor to be shut down for the statutory electrical testing. As a consequence, ALL microscopes in rooms 408/409 will be plugged off during the WE and will not be accessible. The microscopes affected will be: CF3, CF4, CF5, WF1 and WF2. In addition, we will have the annual PAT testing on Monday morning, from 9-11, after which, you can image as before (hopefully). CF2 and the Elyra SR1 are not affected by the electrical testing this WE - but will be PAT-tested on Monday as well. Note: CF4 has now its newly upgraded multiphoton connected to it, and it is working fine and independently of CF3. The Multiphoton on CF3 is not functional as we are having some stability issues. This often happens with brand new lasers, and spectraphysics engineers will come next week to check it out. Cheers Debbi Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00547] REMINDER: Data analysis/modelling needs for FILM
by Keller, Debora 11 Mar '15

11 Mar '15

Dear FILM-Users, Just a reminder, if you could answer to the questions below by Friday 13th March 2015, if you haven't already done so, it would be much appreciated! Cheers Debbi ----------------------------------------------------------------------------------------------------------------- We have the opportunity to recruit a high level data analysis/modelling expert for a trial period and we need to gauge the potential demand. The candidate has experience in high-content screening, and data analysis on different levels, and good data analysis skills can help make the most of your images. If you have any realistic interest in using this person's skills please reply to the following questions within a week: 1) Do you now or in the near future have a need for such skills? 2) Can you estimate usage in hours? 3) How much per hour would you be willing to pay? 4) Would you be willing to include such usage or possibly a part post in grant applications? 5) Would you agree to include this salary in the overall FILM costs? (ie it would be recovered via microscope user fees, as are all FILM staff salaries - maximum 3-5£/hour increase to current rates) Thanks! Debbi & Tony Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00546] TODAY: LSM 8 and Airy Scan presentation 4:30pm room 119 SAF
by Keller, Debora 11 Mar '15

11 Mar '15

Dear FILM-users, Quick reminder: Zeiss will be giving a presentation on their new LSM8 confocal series and the new Airyscan technologies TODAY: Wednesday 11/03 at 4:30 pm in SAF Buidling room 119 TOMORROW: Hammersmith campus, Thursday 12/03 at 4:30 pm in room 427/428 in the ICTEM Building It would be great to see you there! Best wishes, Debbi --------------------------------------------------------------------------------------------------------------------------------------------------- ZEISS is launching the LSM 8 Family with Airyscan - new laser scanning confocal microscopes which offer high sensitivity, high speed and improved resolution in X, Y and Z. More details on the instrument are at: http://www.zeiss.com/lsm880 or http://www.zeiss.com/lsm800 We have two seminars planned - South Kensington campus: Wednesday 11/03 at 4:30 pm in SAF Buidling room 119 - Hammersmith campus: Thursday 12/03 at 4:30 pm in room 427/428 in the ICTEM Building. The seminar will be quite short - about 40 minutes - with the aim of introducing the Airyscan technology and discussing the principle of how it works and giving some application examples. In addition, we will discuss the major points of the LSM 8 family and how it differs from the predecessor instruments. Following the seminar, we will have some refreshments and provide an opportunity to address any questions that may arise. It would be great to see you if you are able to join us. Please feel free to mention it to any of your colleagues who may be interested in attending. It would be helpful if you and colleagues could let us know if you are able to join us so that we can confirm numbers for catering - please email dan.phillips(a)zeiss.com<mailto:dan.phillips@zeiss.com> Many thanks David. Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00545] Zeiss LSM8 and Airyscan introduction NEXT WEEK
by Keller, Debora 06 Mar '15

06 Mar '15

Dear FILM-users, Zeiss will be giving a presentation on their new LSM8 confocal series and the new Airyscan technologies (see below) at both South Ken and Hammersmith campus. It would be great to see you there! Best wishes, Debbi --------------------------------------------------------------------------------------------------------------------------------------------------- ZEISS is launching the LSM 8 Family with Airyscan - new laser scanning confocal microscopes which offer high sensitivity, high speed and improved resolution in X, Y and Z. More details on the instrument are at: http://www.zeiss.com/lsm880 or http://www.zeiss.com/lsm800 We have two seminars planned - South Kensington campus: Wednesday 11/03 at 4:30 pm in SAF Buidling room 119 - Hammersmith campus: Thursday 12/03 at 4:30 pm in room 427/428 in the ICTEM Building. The seminar will be quite short - about 40 minutes - with the aim of introducing the Airyscan technology and discussing the principle of how it works and giving some application examples. In addition, we will discuss the major points of the LSM 8 family and how it differs from the predecessor instruments. Following the seminar, we will have some refreshments and provide an opportunity to address any questions that may arise. It would be great to see you if you are able to join us. Please feel free to mention it to any of your colleagues who may be interested in attending. It would be helpful if you and colleagues could let us know if you are able to join us so that we can confirm numbers for catering - please email dan.phillips(a)zeiss.com<mailto:dan.phillips@zeiss.com> Many thanks David. ------------------------------------------------------------------------------------------------------------------------------------------------------------- Carl Zeiss Limited Division of Microscopy D a v i d C r o o k Ph. D. 3D Imaging Specialist - Team Leader Mobile: +44 (0) 7713 784272 Customer Care Team: +44 (0) 1223 401540 Fax: +44 (0) 1223 401501 mailto: david.crook(a)zeiss.com<mailto:david.crook@zeiss.com> http://www.zeiss.co.uk/

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[FILM-Users 00544] Data analysis/modelling needs for FILM
by Keller, Debora 05 Mar '15

05 Mar '15

Dear FILM-Users, We have the opportunity to recruit a high level data analysis/modelling expert for a trial period and we need to gauge the potential demand. The candidate has experience in high-content screening, and data analysis on different levels, and good data analysis skills can help make the most of your images. If you have any realistic interest in using this person's skills please reply to the following questions within a week: 1) Do you now or in the near future have a need for such skills? 2) Can you estimate usage in hours? 3) How much per hour would you be willing to pay? 4) Would you be willing to include such usage or possibly a part post in grant applications? 5) Would you agree to include this salary in the overall FILM costs? (ie it would be recovered via microscope user fees, as are all FILM staff salaries - maximum 3-5£/hour increase to current rates) Thanks! Debbi & Tony Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00543] Beautiful single cell image needed
by Keller, Debora 01 Mar '15

01 Mar '15

Dear FILM-users, There is a need for a beautiful image of a "cancer" cell (or similar or a cell stained for a protein involved in cancer) for an exhibit of the Proxomics research project at the Royal Society summer exhibit. (see email below). There are some ideas for such images, but I am sure you have much more beautiful images than that! :-) If you have such an image, can you send it to me with a descriptive text (as well as your name and affiliation)? If you could send it before tuesday evening it would be great. The deadline for the deciding on an image is Thursday 5th of march. Cheers Debbi ________________________________ Dear FILM, Our Proxomics research project has been selected for exhibiting at the Royal Society Summer Exhibition this summer under the title “Single Cell Science” and we are looking for a beautiful single cell image for our design template on the stand, flyers and webpage. Would it be possible to circulate an email to ask the FILM community whether anyone has a suitable image that they would be happy for us to use? Best wishes, Natalia Dr. Natalia Goehring Proxomics Project Manager Institute of Chemical Biology | Imperial College London Imperial College Road | SW7 2AZ | UK Tel.: +44 (0)20 7594 3101 Mob.: +44 (0)7872 850 219 Email: n.goehring(a)imperial.ac.uk<mailto:n.goehring@imperial.ac.uk> Proxomics (PROXO) Project: http://www.proxomics.ac.uk/ Institute of Chemical Biology: http://www.chemicalbiology.ac.uk<http://www.chemicalbiology.ac.uk/> [X] Here are some ideas we have found from commercial databases that could be used to depict “Single Cell Science”: [Description: Description: cid:image001.jpg@01D05050.D4F55CC0][Description: cid:image003.jpg@01D05050.D4F55CC0][Description: cid:image012.jpg@01D05050.D4F55CC0][Description: cid:image013.jpg@01D05050.D4F55CC0]

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