February 2015 - FILM-Users

[FILM-Users 00542] TODAY: LSR meeting at 4pm SAF building Imperial College
by Keller, Debora 26 Feb '15

26 Feb '15

Dear all, Just a reminder that our LSR meeting is taking place today at Imperial College SAF building at 4pm. Afterwards, we will continue our discussions at the Queen's Arms (where it all began - 6 min from Imperial<https://www.google.co.uk/maps/place/30+Queen's+Gate+Mews,+Kensington,+London+SW7+5QL/@51.499246,-0.180824,17z/data=!3m1!4b1!4m2!3m1!1s0x4876055bf5b22663:0x88e17b20924adbb6> ) Looking forward to some nice images & discussions! Cheers Debbi, Dylan and Ricardo ----------------------------------------------------------------------------------------------------------------------------------------------------------------------- Dear SR-afficionados, It is time for another session filled with interesting super-res talks! We even managed to cover all (main) methods: SIM, STED, STORM and TIRF within our 3 distinguished speakers!! Who: - Jason Mercer (MRC-LMCB, University College London): 'How to build a virus...A really big virus' - Siân Culley (MRC-LMCB, University College London): "Smaller, Better, Faster, (yet not) Stronger" - George Ashdown (EBN, King's College London): "Dynamics of the Actin Cytoskeleton and Plasma Membrane at the Immunological Synapse using live-cell super resolution microscopy" When: 26th of February 4pm to 5:30pm. Where: G34 lecture theatre, Sir Alexander Fleming building, South Kensington, Imperial College London (link to google maps here<https://www.google.com/maps/place/Sir+Alexander+Fleming+Bldg,+Kensington,+L…>) BONUS: To take full advantage of the wide range of experts present at this meeting, you, the audience, will have to opportunity to submit your questions/problems that you are facing in super-res. Surely someone will be able to help you out, and who knows, this could be the beginning of a wonderful scientific collaboration! :) If you already have some questions in mind, please send them to us (Dylan, Ricardo and myself) ahead of the meeting so we can already brainstorm a bit! And, in the great tradition of the LSR meetings, we will continue our scientific and non-scientific socializing in a nearby pub (still TBD). Looking forward to seeing you there! Debbi, Dylan & Ricardo Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00541] NEXT WEEK: HCS Demo Olympus ScanR
by Keller, Debora 25 Feb '15

25 Feb '15

Dear users, Some of you have voiced interest in demoing the microscopes for high-content imaging - thank you very much. The first demo will be next week, starting Tuesday 3rd March with the Olympus ScanR and the CellSens software (see flyers attached). You can book a slot using the "booking diary" on Scratch \\film-nas.med.ic.ac.uk\scratch\_HCS Demo<file:///\\film-nas.med.ic.ac.uk\scratch\_HCS%20Demo> Olympus will have one of their specialist from Germany during the first few days (Tuesday-Wednedsay), so take advantage of those days! ? If you won't be there during the week but still want someone to demo your samples, shoot us an email In preparation for the demo, can you also fill out the attached document with as much detail as you can on your sample, the microscope you are currently using, the current analysis workflow, and the "ideal" output from the images. You can put a sample image in scratch, under the same folder as above (_HCS Demo). ? For those who have contacted us already, can you still fill the form and send it to us? (it will be useful also for the next rounds of demos) If you wish, and haven't already done so, you can come and talk to us. I (debbi) will be available tomorrow morning (9am - 11am) or afternoon (1pm-4pm), or else on Monday 2nd (or send us an email) Also, if some of you are available on Thursday 5th of March at 2pm, Nikon are coming over to discuss imaging and analysis requirements so they can prepare some acquisition and analysis workflow in advance of the demo. The setup they are bringing would be a combined confocal/widefield modality. ? Let me know if you wish to meet them ? If the 5th of march does not work, but you are still interested in meeting them, let me know. Again, if any questions, don't hesitate to contact us! Cheers Debbi Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00540] anti-human Stro-1 antibody
by Keller, Debora 23 Feb '15

23 Feb '15

Can any of you help Mark out? (see below) From: Allenby, Mark Sent: 20 February 2015 13:16 To: Keller, Debora Subject: Stro-1 Missing Dear Debbi, An antibody I have ordered for a clinical sample arriving at the end of next week, is backordered until 5 days later than when my experiment starts. I was wondering if you knew anyone who might have a tiny bit of antibody (any conjugation, host species) for anti-human Stro-1, for mesenchymal stem cells. Enough for a single flow cytometry run. Thanks for your help! Mark

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[FILM-Users 00539] High-Content Screening: FILM needs your samples!
by Keller, Debora 16 Feb '15

16 Feb '15

Dear users, As you remember, we have been awarded a Wellcome Trust grant to purchase a high-content microscope. We are now in the process of organizing demos and for that we need you (and your samples)! Is high-content for you? If you are imaging multi-well plates to follow gene-expression, or trying to analyse multiple cell populations after drug treatment, doing some small-scale RNAi screens, imaging nano-particle uptake, following spheroids or organoids or even tracking several fish over time: this is high-content imaging, and your samples will be invaluable to test the flexibility and functionality of the setups as well as the analysis. Please send us an email if you wish to provide samples, with a short description of your experiment, dye/fluorescent protein type, support type (multi-well plate, luer cell, slide, ...), environmental conditions, flow, expected outcome, or just come to chat with us. The more varied the sample types, the more thoroughly we can test both the acquisition setup and the analysis packages. So far, we are going to test several microscopes/platforms: - ScanR from Olympus (2-6th March, SAF): microscope setup - Operetta from Perkin Elmer (28-29th April, SAF): confocal modality, box setup - ImageXpress Ultra from Molecular Devices (demo dates TBC, SAF): box setup - Nikon WF/confocal (demo dates TBC): dual modality on microscope setup Let us know, preferentially by the end of this week! :) Debbi & Steve Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00538] FYI: Next LSR-meeting 26th February 2015, 4pm, South Ken
by Keller, Debora 06 Feb '15

06 Feb '15

Dear FILM-users, My apologies for those who will receive this message twice - but below an invitation to attend our upcoming London Super-Resolution meeting. You don't need to be doing super-res to attend, just to be interested in it :) The talks can give you an example of what can be done in SR. Most methods are available at FILM (PALM, SIM,STORM, TIRF) or at Imperial (photonics). Looking forward to seeing you there! Cheers Debbi From: lsr-group-bounces(a)imperial.ac.uk [mailto:lsr-group-bounces@imperial.ac.uk] On Behalf Of Keller, Debora Sent: 06 February 2015 16:42 To: lsr-group Subject: [lsr-group] Next LSR-meeting 26th February 2015, 4pm, South Ken Dear SR-afficionados, It is time for another session filled with interesting super-res talks! We even managed to cover all (main) methods: SIM, STED, STORM and TIRF within our 3 distinguished speakers!! Who: - Jason Mercer (MRC-LMCB, University College London): 'How to build a virus...A really big virus' - Siân Culley (MRC-LMCB, University College London): "Smaller, Better, Faster, (yet not) Stronger" - George Ashdown (EBN, King's College London): "Dynamics of the Actin Cytoskeleton and Plasma Membrane at the Immunological Synapse using live-cell super resolution microscopy" When: 26th of February 4pm to 5:30pm. Where: G34 lecture theatre, Sir Alexander Fleming building, South Kensington, Imperial College London (link to google maps here<https://www.google.com/maps/place/Sir+Alexander+Fleming+Bldg,+Kensington,+L…>) BONUS: To take full advantage of the wide range of experts present at this meeting, you, the audience, will have to opportunity to submit your questions/problems that you are facing in super-res. Surely someone will be able to help you out, and who knows, this could be the beginning of a wonderful scientific collaboration! :) If you already have some questions in mind, please send them to us (Dylan, Ricardo and myself) ahead of the meeting so we can already brainstorm a bit! And, in the great tradition of the LSR meetings, we will continue our scientific and non-scientific socializing in a nearby pub (still TBD). Looking forward to seeing you there! Debbi, Dylan & Ricardo Debora Keller, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Super-Resolution Specialist - Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ, UK Phone: +44 (0)207 594 2023 Mobile: + 44(0) 7760 256 889 E-mail: d.keller(a)imperial.ac.uk<mailto:d.keller@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00537] Reminder TODAY Advanced imaging seminar - Aleksandar Ivetic
by Keller, Debora 03 Feb '15

03 Feb '15

Dear All, The research seminar series this term continues with: Who? Aleksandar Ivetic (Cardiovascular Division, King's College, London) Subject? "Advanced imaging techniques reveal a novel role for L-selectin in regulating transendothelial migration and cell polarity" Where? Room 120, SAF When? Tuesday 3rd February, 12-1pm All are welcome to attend. Many thanks, Tanya Dr Tanya Chong Section Administrator, NHLI Sir Alexander Fleming Building Imperial College South Kensington London SW7 2AZ

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