June 2014 - FILM-Users - imperial.ac.uk

[FILM-Users 00519] thanks
by Martin Spitaler 30 Jun '14

30 Jun '14

Dear microscopists, at the end of the Microscopy Day everybody was in a rush to go to the drinks, so I would like to give my thanks by email to all who made my time in FILM a really special time: There are too many to mention, but first of course is Tony Magee, who did the hard work to get the money that got FILM of the ground (together with Mike Ferenczi and Dan Davis), and gave me the great opportunity to help it growing to its current strength. Anna McDadd and Ian Morris were always there to help with practical issues regarding the rooms; Pat Evans and her team have been working hard to sort out the organisation and administration; Richard Mattin and his troop have been incredible helpful with all admin tasks; Sarah Butcher and her excellent Bioinformatics Support Service team; and all you microscopists who have been using the microscopes and gave me insights into the most weird, wonderful and spectacular science. A special thanks goes to my colleagues Steve Rothery and Debora Keller, who are incredibly hardworking and dedicated to help people and sort out all problems, often behind the scenes, unnoticed by users. It's been a great time, and although I have now moved on to new challenges (to be more precise, to the MPI for Biochemistry in Munich), I'll always have good memories and hope FILM will keep moving from strength to strength. Meanwhile, if you still need to contact me, I don't have a new address yet, but just use my normal Imperial College email account, this will still work for quite some time. All the best, and much more good imaging, Martin

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[FILM-Users 00518] TOMORROW: FILM Microscopy Day 2014
by Martin Spitaler 24 Jun '14

24 Jun '14

...just a reminder about tomorrow's FILM Microscopy Day: We now have an exciting line-up of speakers for the Microscopy Match Maker session, see full invitation at <https://workspace.imperial.ac.uk/imagingfacility/Public/FILM%20Microscopy%2…> Martin ------------------------------------------------------------------------ -------- Original Message -------- Subject: [FILM-Users 00513] FILM Microscopy Day 2014 - user presentations needed! Date: Tue, 17 Jun 2014 12:30:12 +0100 From: Martin Spitaler <m.spitaler(a)imperial.ac.uk> To: <film-users(a)imperial.ac.uk> Dear Microscopists, for the Microscopy Match Maker session in our Microscopy Day next week, we only have one presentation so far! Everyone really liked this session in previous years, but it only works if people are actually presenting - 2 slides is all that's needed, and it's all about projects and techniques to get people to know each other, you most definitely do NOT need any results (there wouldn't be enough time to show any anyway). So please recycle the last lab meeting and show us what you do. And supervisors, please encourage your students and postdoc to participate. And don't forget to register by email to Ruth O'Donnell <r.odonnell(a)imperial.ac.uk> if you plan to participate, we need the numbers for catering purposes. On that line, anyone who wants to move on later to my leaving drinks, the venue is booked now, it's the Hereford Arms <http://www.herefordarms.co.uk/>, 127 Gloucester Rd, SW7 4TE. We should be there from around 6.30pm. See you next Wednesday (with your presentation!), good imaging, Martin ------------------------------------------------------------------------ Dear microscopists, just a reminder about this year's Microscopy Day: *FILM MICROSCOPY DAY 2014, Wednesday 25 June, SAFB-G34 (South Kensington Campus), 10.00-17.00* Again a whole day full of exciting talks and presentations about microscopy, better and bigger than ever - this time with not one, but two plenary lectures, one by internal microscopist Cristina Lo Celso, the other by Phillip Kukura from Oxford. See full agenda below. And we hope many of you will add to the exciting programme with your own 3-min presenation of the microscopy you are doing in your lab: The Microscopy Matchmaker Session has always been a highlight of the day, and the more presentations we get, the more fun it will be, and the better your chances of getting to know other people's work and starting new collaborations. See also the full invitation at <https://workspace.imperial.ac.uk/imagingfacility/Public/FILM%20Microscopy%2…> Participation is free, but for catering purposes please register replying by email to Ruth O'Donnell <r.odonnell(a)imperial.ac.uk> Martin ------------------------------------------------------------------------ http://www3.imperial.ac.uk/imagingfacility/news#md _*FILM Microscopy Day - Agenda *_ *_Morning Session_*** *10.00-10.15Welcome and latest FILM News (Tony Magee) *New microscopes, new equipment, new staff *10.15-11.15 Basics of microscopy (Martin Spitaler, FILM) *Image formation, fluorescence, visualisation, quantitation */11.15-11.35 coffee & tea break/*** *11.35-12.15 Sample preparation for live and fixed samples (Stephen Rothery, FILM) *Fixatives, strategies, viability, autofluorescence ** *_Microscopy Match-Maker_*** *12.15-13.00 Microscopy Match-Maker *Research performed in FILM, presented by users through 3 min snapshot presentations */13.00-14.00 Sandwich lunch /*/with opportunity to see the new super-resolution microscope / *_Afternoon Session_*** *14.00-14.40 Super-resolution microscopy (Debora Keller, FILM) *PALM, STORM, SIM, resolution limits, artefacts */14.40-15.00 coffee & tea break/*** *_PLENARY SESSION _*** *15.00-15.55Cristina Lo Celso, Imperial College: /"Intra-vital imaging in stem cell biology"/* *15.55-16.50 Philipp Kukura, Oxford/: "Molecular Imaging"/* *16.50-17.00 Tony Magee: Round-up 17.00-17.30 Refreshments * ** *FILM Microscopy Day 2014: Plenary session*** *Cristina Lo Celso, Imperial College*: */"Intra-vital imaging in stem cell biology"/* After a PhD from University College London in the field of epidermal stem cell biology, Dr Lo Celso worked as a postdoc at Harvard University developing intra-vital microscopy of the haematopoietic stem cell niche. In 2009 she was appointed assistant professor at Imperial College London, where she has established a research group focussing on understanding the cellular mechanisms regulating haematopoietic stem cell (HSC) function and leukaemia development and . In close collaboration with FILM, her group has developed tools to study HSCs in their native bone marrow environment. She will present an interdisciplinary approach combining intra-vital microscopy techniques, computational image analysis, molecular profiling and mathematical modelling and the group's most recent observations of the dynamic interactions between HSCs and the bone marrow microenvironment. *Philipp Kukura, Oxford/: "Molecular Imaging" /* The primary goal of optical microscopy is to visualise microscopic structure and dynamics. Developments over the past decades have enabled routine studies down to the single molecule level and structural observations far beyond the diffraction limit through the use of fluorescence as a contrast mechanism. Despite its many advantages, one of the fundamental limitations of fluorescence detection is the frequency with which photons can be emitted and thus detected. As a consequence, imaging speeds remain limited to few to tens of frames per resulting in a considerable gap between the speed at which dynamics can be recorded and the speed of motion on the nanoscale. I will describe an alternative approach to optical microscopy that relies on the ultra-efficient detection of light scattering called interferometric scattering microscopy (iSCAT). I will show that iSCAT can follow the motion of nanoscopic labels with nm precision down to the microsecond regime, the relevant timescale for a majority of dynamics on the nanoscale. Thereby, we are able to address a surprising variety of fundamental questions ranging from the mechanism underlying processive molecular motion to the existence and formation of lipid rafts in membranes. Finally, I will show that iSCAT can detect, image and track the motion of single, proteins using light scattering without the need for any labels. _* *_ -- ------------------------------------------------------------------------ Please note that I'm leaving Imperial College on 25 June 2014. For requests after that date please contact film-service(a)imperial.ac.uk <mailto:film-service@imperial.ac.uk> *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00517] TOMORROW: FILM Microscopy Day 2014
by Martin Spitaler 24 Jun '14

24 Jun '14

...just a reminder about tomorrow's FILM Microscopy Day: We now have an exciting line-up of speakers for the Microscopy Match Maker session, see full invitation at <https://workspace.imperial.ac.uk/imagingfacility/Public/FILM%20Microscopy%2…> Martin ------------------------------------------------------------------------ -------- Original Message -------- Subject: [FILM-Users 00513] FILM Microscopy Day 2014 - user presentations needed! Date: Tue, 17 Jun 2014 12:30:12 +0100 From: Martin Spitaler <m.spitaler(a)imperial.ac.uk> To: <film-users(a)imperial.ac.uk> Dear Microscopists, for the Microscopy Match Maker session in our Microscopy Day next week, we only have one presentation so far! Everyone really liked this session in previous years, but it only works if people are actually presenting - 2 slides is all that's needed, and it's all about projects and techniques to get people to know each other, you most definitely do NOT need any results (there wouldn't be enough time to show any anyway). So please recycle the last lab meeting and show us what you do. And supervisors, please encourage your students and postdoc to participate. And don't forget to register by email to Ruth O'Donnell <r.odonnell(a)imperial.ac.uk> if you plan to participate, we need the numbers for catering purposes. On that line, anyone who wants to move on later to my leaving drinks, the venue is booked now, it's the Hereford Arms <http://www.herefordarms.co.uk/>, 127 Gloucester Rd, SW7 4TE. We should be there from around 6.30pm. See you next Wednesday (with your presentation!), good imaging, Martin ------------------------------------------------------------------------ Dear microscopists, just a reminder about this year's Microscopy Day: *FILM MICROSCOPY DAY 2014, Wednesday 25 June, SAFB-G34 (South Kensington Campus), 10.00-17.00* Again a whole day full of exciting talks and presentations about microscopy, better and bigger than ever - this time with not one, but two plenary lectures, one by internal microscopist Cristina Lo Celso, the other by Phillip Kukura from Oxford. See full agenda below. And we hope many of you will add to the exciting programme with your own 3-min presenation of the microscopy you are doing in your lab: The Microscopy Matchmaker Session has always been a highlight of the day, and the more presentations we get, the more fun it will be, and the better your chances of getting to know other people's work and starting new collaborations. See also the full invitation at <https://workspace.imperial.ac.uk/imagingfacility/Public/FILM%20Microscopy%2…> Participation is free, but for catering purposes please register replying by email to Ruth O'Donnell <r.odonnell(a)imperial.ac.uk> Martin ------------------------------------------------------------------------ http://www3.imperial.ac.uk/imagingfacility/news#md _*FILM Microscopy Day - Agenda *_ *_Morning Session_*** *10.00-10.15Welcome and latest FILM News (Tony Magee) *New microscopes, new equipment, new staff *10.15-11.15 Basics of microscopy (Martin Spitaler, FILM) *Image formation, fluorescence, visualisation, quantitation */11.15-11.35 coffee & tea break/*** *11.35-12.15 Sample preparation for live and fixed samples (Stephen Rothery, FILM) *Fixatives, strategies, viability, autofluorescence ** *_Microscopy Match-Maker_*** *12.15-13.00 Microscopy Match-Maker *Research performed in FILM, presented by users through 3 min snapshot presentations */13.00-14.00 Sandwich lunch /*/with opportunity to see the new super-resolution microscope / *_Afternoon Session_*** *14.00-14.40 Super-resolution microscopy (Debora Keller, FILM) *PALM, STORM, SIM, resolution limits, artefacts */14.40-15.00 coffee & tea break/*** *_PLENARY SESSION _*** *15.00-15.55Cristina Lo Celso, Imperial College: /"Intra-vital imaging in stem cell biology"/* *15.55-16.50 Philipp Kukura, Oxford/: "Molecular Imaging"/* *16.50-17.00 Tony Magee: Round-up * ** *FILM Microscopy Day 2014: Plenary session*** *Cristina Lo Celso, Imperial College*: */"Intra-vital imaging in stem cell biology"/* After a PhD from University College London in the field of epidermal stem cell biology, Dr Lo Celso worked as a postdoc at Harvard University developing intra-vital microscopy of the haematopoietic stem cell niche. In 2009 she was appointed assistant professor at Imperial College London, where she has established a research group focussing on understanding the cellular mechanisms regulating haematopoietic stem cell (HSC) function and leukaemia development and . In close collaboration with FILM, her group has developed tools to study HSCs in their native bone marrow environment. She will present an interdisciplinary approach combining intra-vital microscopy techniques, computational image analysis, molecular profiling and mathematical modelling and the group's most recent observations of the dynamic interactions between HSCs and the bone marrow microenvironment. *Philipp Kukura, Oxford/: "Molecular Imaging" /* The primary goal of optical microscopy is to visualise microscopic structure and dynamics. Developments over the past decades have enabled routine studies down to the single molecule level and structural observations far beyond the diffraction limit through the use of fluorescence as a contrast mechanism. Despite its many advantages, one of the fundamental limitations of fluorescence detection is the frequency with which photons can be emitted and thus detected. As a consequence, imaging speeds remain limited to few to tens of frames per resulting in a considerable gap between the speed at which dynamics can be recorded and the speed of motion on the nanoscale. I will describe an alternative approach to optical microscopy that relies on the ultra-efficient detection of light scattering called interferometric scattering microscopy (iSCAT). I will show that iSCAT can follow the motion of nanoscopic labels with nm precision down to the microsecond regime, the relevant timescale for a majority of dynamics on the nanoscale. Thereby, we are able to address a surprising variety of fundamental questions ranging from the mechanism underlying processive molecular motion to the existence and formation of lipid rafts in membranes. Finally, I will show that iSCAT can detect, image and track the motion of single, proteins using light scattering without the need for any labels. _* *_ -- ------------------------------------------------------------------------ Please note that I'm leaving Imperial College on 25 June 2014. For requests after that date please contact film-service(a)imperial.ac.uk <mailto:film-service@imperial.ac.uk> *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00516] Re: visit to access possible options on TCS SP5 systems
by Martin Spitaler 20 Jun '14

20 Jun '14

Hi Paul, I've copied all other contacts in: Steve Rothery and Debora Keller, plus the <film-service(a)imperial.ac.uk>, which actually goes to those two as well. Or phone ++44(0)20-759-49793. All the best, Martin On 20/06/2014 14:09, Suckling, Paul wrote: > > Hi Martin > > I have just left you a voice message about the mail below as I was > wanting to come in and see you next Thursday to look at and discussion > the possibility for moving 405nm and MP parts around the systems , but > then of course I notice you are actually leaving on the 25^th , so we > would miss each other . > > I am really committed elsewhere until next Thursday so do you have a > name of someone I could contact about the request ? > > Really sorry for this , but just come back to me and lets see what can > be arranged. > > Regards and all the very best for the future . > > Paul > > ------------------------------------------------------------------------ > > > > > Dear Leica Service, > > in the facility, we have 3 Leica SP5 systems under full service contract: > > o serial number 5100000307: SP5 multiphoton upright > o serial number 5100000293: SP5 multiphoton inverted > o serial number 5100000291: SP5 resonant > > In a room next door, there is an under-used SP2-multiphoton system > (serial number 1428000528), which is getting old and needs to make > space for new equipment. But the microscope is still fully functional, > so we would like to explore options to re-use part on existing equipment: > > o the Mai Tai multiphoton laser could be used on the inverted > multi-photon SP5 system (currently, both multiphoton systems > share one laser) > o the motorised stage could be used on the resonant SP5 system > o the 405nm laser could be used on the upright multi-photon SP5 > system > o the microscope stand and optical table would be used in other labs > > We therefore need two bits of information: First, we need a Leica > engineer to come in and check compatibility (physical dimensions, > Windows-7 64b drivers etc). Once that's established, we will then need > a quote about costs for the work involved. > > Could you please first send out an engineer to check the technical > feasibility? > > Best regards, > > Martin > -- > > ------------------------------------------------------------------------ > > Please note that I'm leaving Imperial College on 25 June 2014. For > requests after that date please contact film-service(a)imperial.ac.uk > <mailto:film-service@imperial.ac.uk> > * > Martin Spitaler, PhD > FILM - Facility for Imaging by Light Microscopy* > - Facility Manager - > Sir Alexander Fleming Building, desk 401 > Imperial College London / South Kensington > Exhibition Road > London SW7 2AZ > UK > > Tel. +44-(0)20-759-42023 > E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> > Website: http://imperial.ac.uk/imagingfacility > > > Please be advised that this email may contain confidential > information. If you are not the intended recipient, please notify us > by email by replying to the sender and delete this message. The sender > disclaims that the content of this email constitutes an offer to enter > into, or the acceptance of, any agreement; provided that the foregoing > does not invalidate the binding effect of any digital or other > electronic reproduction of a manual signature that is included in any > attachment.

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[FILM-Users 00514] Martin leaving card
by Rothery, Stephen M 20 Jun '14

20 Jun '14

Dear All As you will probably know Martin Spitaler is leaving us There is a card if you wish to add your appreciation on desk 408 (Debbi's Desk) Level 4 SAF (first row of desks at the front of the building) Also if you have any photos of Martin's time here that we can add to an album, please can you send then to either myself or Debbi Many thanks Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1669 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility **************************************************************

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[FILM-Users 00513] FILM Microscopy Day 2014 - user presentations needed!
by Martin Spitaler 17 Jun '14

17 Jun '14

Dear Microscopists, for the Microscopy Match Maker session in our Microscopy Day next week, we only have one presentation so far! Everyone really liked this session in previous years, but it only works if people are actually presenting - 2 slides is all that's needed, and it's all about projects and techniques to get people to know each other, you most definitely do NOT need any results (there wouldn't be enough time to show any anyway). So please recycle the last lab meeting and show us what you do. And supervisors, please encourage your students and postdoc to participate. And don't forget to register by email to Ruth O'Donnell <r.odonnell(a)imperial.ac.uk> if you plan to participate, we need the numbers for catering purposes. On that line, anyone who wants to move on later to my leaving drinks, the venue is booked now, it's the Hereford Arms <http://www.herefordarms.co.uk/>, 127 Gloucester Rd, SW7 4TE. We should be there from around 6.30pm. See you next Wednesday (with your presentation!), good imaging, Martin ------------------------------------------------------------------------ Dear microscopists, just a reminder about this year's Microscopy Day: *FILM MICROSCOPY DAY 2014, Wednesday 25 June, SAFB-G34 (South Kensington Campus), 10.00-17.00* Again a whole day full of exciting talks and presentations about microscopy, better and bigger than ever - this time with not one, but two plenary lectures, one by internal microscopist Cristina Lo Celso, the other by Phillip Kukura from Oxford. See full agenda below. And we hope many of you will add to the exciting programme with your own 3-min presenation of the microscopy you are doing in your lab: The Microscopy Matchmaker Session has always been a highlight of the day, and the more presentations we get, the more fun it will be, and the better your chances of getting to know other people's work and starting new collaborations. See also the full invitation at <https://workspace.imperial.ac.uk/imagingfacility/Public/FILM%20Microscopy%2…> Participation is free, but for catering purposes please register replying by email to Ruth O'Donnell <r.odonnell(a)imperial.ac.uk> Martin ------------------------------------------------------------------------ http://www3.imperial.ac.uk/imagingfacility/news#md _*FILM Microscopy Day - Agenda *_ *_Morning Session_*** *10.00-10.15Welcome and latest FILM News (Tony Magee) *New microscopes, new equipment, new staff *10.15-11.15 Basics of microscopy (Martin Spitaler, FILM) *Image formation, fluorescence, visualisation, quantitation */11.15-11.35 coffee & tea break/*** *11.35-12.15 Sample preparation for live and fixed samples (Stephen Rothery, FILM) *Fixatives, strategies, viability, autofluorescence ** *_Microscopy Match-Maker_*** *12.15-13.00 Microscopy Match-Maker *Research performed in FILM, presented by users through 3 min snapshot presentations */13.00-14.00 Sandwich lunch /*/with opportunity to see the new super-resolution microscope / *_Afternoon Session_*** *14.00-14.40 Super-resolution microscopy (Debora Keller, FILM) *PALM, STORM, SIM, resolution limits, artefacts */14.40-15.00 coffee & tea break/*** *_PLENARY SESSION _*** *15.00-15.55Cristina Lo Celso, Imperial College: /"Intra-vital imaging in stem cell biology"/* *15.55-16.50 Philipp Kukura, Oxford/: "Molecular Imaging"/* *16.50-17.00 Tony Magee: Round-up * ** *FILM Microscopy Day 2014: Plenary session*** *Cristina Lo Celso, Imperial College*: */"Intra-vital imaging in stem cell biology"/* After a PhD from University College London in the field of epidermal stem cell biology, Dr Lo Celso worked as a postdoc at Harvard University developing intra-vital microscopy of the haematopoietic stem cell niche. In 2009 she was appointed assistant professor at Imperial College London, where she has established a research group focussing on understanding the cellular mechanisms regulating haematopoietic stem cell (HSC) function and leukaemia development and . In close collaboration with FILM, her group has developed tools to study HSCs in their native bone marrow environment. She will present an interdisciplinary approach combining intra-vital microscopy techniques, computational image analysis, molecular profiling and mathematical modelling and the group's most recent observations of the dynamic interactions between HSCs and the bone marrow microenvironment. *Philipp Kukura, Oxford/: "Molecular Imaging" /* The primary goal of optical microscopy is to visualise microscopic structure and dynamics. Developments over the past decades have enabled routine studies down to the single molecule level and structural observations far beyond the diffraction limit through the use of fluorescence as a contrast mechanism. Despite its many advantages, one of the fundamental limitations of fluorescence detection is the frequency with which photons can be emitted and thus detected. As a consequence, imaging speeds remain limited to few to tens of frames per resulting in a considerable gap between the speed at which dynamics can be recorded and the speed of motion on the nanoscale. I will describe an alternative approach to optical microscopy that relies on the ultra-efficient detection of light scattering called interferometric scattering microscopy (iSCAT). I will show that iSCAT can follow the motion of nanoscopic labels with nm precision down to the microsecond regime, the relevant timescale for a majority of dynamics on the nanoscale. Thereby, we are able to address a surprising variety of fundamental questions ranging from the mechanism underlying processive molecular motion to the existence and formation of lipid rafts in membranes. Finally, I will show that iSCAT can detect, image and track the motion of single, proteins using light scattering without the need for any labels. _* *_ -- ------------------------------------------------------------------------ Please note that I'm leaving Imperial College on 25 June 2014. For requests after that date please contact film-service(a)imperial.ac.uk <mailto:film-service@imperial.ac.uk> *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00512] Re: LacO Array
by Martin Spitaler 16 Jun '14

16 Jun '14

...forwarded on behalf of Michael - can anybody help? Best regards, Martin On 16/06/2014 14:58, Clarke, Michael wrote: > Hi Martin, > > i was wondering if you could email around and ask if anybody has a > plasmid containing an array of 128-256 LacO repeats? > > Many thanks > Michael

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[FILM-Users 00511] FILM Microscopy Day 2014
by Martin Spitaler 12 Jun '14

12 Jun '14

Dear microscopists, just a reminder about this year's Microscopy Day: *FILM MICROSCOPY DAY 2014, Wednesday 25 June, SAFB-G34 (South Kensington Campus), 10.00-17.00* Again a whole day full of exciting talks and presentations about microscopy, better and bigger than ever - this time with not one, but two plenary lectures, one by internal microscopist Cristina Lo Celso, the other by Phillip Kukura from Oxford. See full agenda below. And we hope many of you will add to the exciting programme with your own 3-min presenation of the microscopy you are doing in your lab: The Microscopy Matchmaker Session has always been a highlight of the day, and the more presentations we get, the more fun it will be, and the better your chances of getting to know other people's work and starting new collaborations. See also the full invitation at <https://workspace.imperial.ac.uk/imagingfacility/Public/FILM%20Microscopy%2…> Participation is free, but for catering purposes please register replying by email to Ruth O'Donnell <r.odonnell(a)imperial.ac.uk> Martin PS: as most of you will know by now, the Microscopy Day will also be my last day in this job before I move on, so these will also be my leaving drinks; more hardy thinkers or drinkers will probably move on to a pub nearby, tbc PPS: I know people are traditionally keen on leaving presents; to make your life easier, I propose that - unless you have an idea for something small and symbolic (easy to take on a plane) - you donate any collected money to the homeless charity <http://www.mungosbroadway.org.uk> - they are doing a really good job (and with Imperial's staff policy, you never know when you need them...) ------------------------------------------------------------------------ http://www3.imperial.ac.uk/imagingfacility/news#md _*FILM Microscopy Day - Agenda *_ *_Morning Session_*** *10.00-10.15Welcome and latest FILM News (Tony Magee) *New microscopes, new equipment, new staff *10.15-11.15 Basics of microscopy (Martin Spitaler, FILM) *Image formation, fluorescence, visualisation, quantitation */11.15-11.35 coffee & tea break/*** *11.35-12.15 Sample preparation for live and fixed samples (Stephen Rothery, FILM) *Fixatives, strategies, viability, autofluorescence ** *_Microscopy Match-Maker_*** *12.15-13.00 Microscopy Match-Maker *Research performed in FILM, presented by users through 3 min snapshot presentations */13.00-14.00 Sandwich lunch /*/with opportunity to see the new super-resolution microscope / *_Afternoon Session_*** *14.00-14.40 Super-resolution microscopy (Debora Keller, FILM) *PALM, STORM, SIM, resolution limits, artefacts */14.40-15.00 coffee & tea break/*** *_PLENARY SESSION _*** *15.00-15.55Cristina Lo Celso, Imperial College: /"Intra-vital imaging in stem cell biology"/* *15.55-16.50 Philipp Kukura, Oxford/: "Molecular Imaging"/* *16.50-17.00 Tony Magee: Round-up * ** *FILM Microscopy Day 2014: Plenary session*** *Cristina Lo Celso, Imperial College*: */"Intra-vital imaging in stem cell biology"/* After a PhD from University College London in the field of epidermal stem cell biology, Dr Lo Celso worked as a postdoc at Harvard University developing intra-vital microscopy of the haematopoietic stem cell niche. In 2009 she was appointed assistant professor at Imperial College London, where she has established a research group focussing on understanding the cellular mechanisms regulating haematopoietic stem cell (HSC) function and leukaemia development and . In close collaboration with FILM, her group has developed tools to study HSCs in their native bone marrow environment. She will present an interdisciplinary approach combining intra-vital microscopy techniques, computational image analysis, molecular profiling and mathematical modelling and the group's most recent observations of the dynamic interactions between HSCs and the bone marrow microenvironment. *Philipp Kukura, Oxford/: "Molecular Imaging" /* The primary goal of optical microscopy is to visualise microscopic structure and dynamics. Developments over the past decades have enabled routine studies down to the single molecule level and structural observations far beyond the diffraction limit through the use of fluorescence as a contrast mechanism. Despite its many advantages, one of the fundamental limitations of fluorescence detection is the frequency with which photons can be emitted and thus detected. As a consequence, imaging speeds remain limited to few to tens of frames per resulting in a considerable gap between the speed at which dynamics can be recorded and the speed of motion on the nanoscale. I will describe an alternative approach to optical microscopy that relies on the ultra-efficient detection of light scattering called interferometric scattering microscopy (iSCAT). I will show that iSCAT can follow the motion of nanoscopic labels with nm precision down to the microsecond regime, the relevant timescale for a majority of dynamics on the nanoscale. Thereby, we are able to address a surprising variety of fundamental questions ranging from the mechanism underlying processive molecular motion to the existence and formation of lipid rafts in membranes. Finally, I will show that iSCAT can detect, image and track the motion of single, proteins using light scattering without the need for any labels. _* *_ -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK

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[FILM-Users 00510] Fwd: Seminar/Interview clash 20/06/2014: Ad hoc Life Sciences seminar
by Martin Spitaler 10 Jun '14

10 Jun '14

...forwarded for your information.... Martin > *From: *DoLSNEWS <dolsnews(a)imperial.ac.uk > <mailto:dolsnews@imperial.ac.uk>> > *Subject: **20/06/2014: Ad hoc Life Sciences seminar* > *Date: *June 9, 2014 4:19:07 PM GMT+01:00 > *To: *DoLS Staff and PGR Students <ls_all_dl(a)imperial.ac.uk > <mailto:ls_all_dl@imperial.ac.uk>> > > > Department of Life Sciences ad hoc seminar > > Speaker: Professor Grant J. Jensen, California Institute of > Technology, USA > > Title: "How electron cryo-tomography is opening a new window on > bacterial cell biology: type VI secretion systems and correlated light > and electron microscopies" > > Date: 12:30pm, Friday 20th June 2014, G47A Flowers Building > > Host: Dr Morgan Beeby >

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[FILM-Users 00509] Fwd: Spare inverted microscope frame
by Martin Spitaler 06 Jun '14

06 Jun '14

...forwarded for your information, Martin -------- Original Message -------- Subject: Re: Spare inverted microscope frame Date: Tue, 3 Jun 2014 10:05:06 +0100 From: Clegg, James <j.clegg11(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> > Hi Martin > > I'm James, one of Mark Neil's students in the Physics department. We > met once about a year ago when I came round to SAF to see your old > flat panel monitors, and I've seen you at quite a few London > SuperResolution meetings. > > Mark and I are trying to build a new microscope with our own confocal > scanning apparatus but, in the true university style, we don't have a > frame to use and we probably can't afford to buy one! Perhaps this is > a bit of a long shot but Mark mentioned that you might have a frame > that we could borrow. Something like an Olympus IX71 would be ideal > as we have a few Olympus objectives already and inverted would be > best. Can you help out? > > Best regards, > > James Clegg

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