February 2014 - FILM-Users

[FILM-Users 00434] FILM application for Wellcome Trust Multi-User Equipment grant
by Martin Spitaler 31 Mar '14

31 Mar '14

Dear microscopists, following feedback from FILM users and Wellcome Trust grant holders (see summary at the bottom), the FILM steering committee has decided to use the open call for Wellcome Trust Multi-User Equipment grant applications to apply for upgrades / updates of existing equipment as well as some novel technology. The highest demand is currently for a new super-resolution microscope, which is luckily already funded by BBSRC, we are now in the process of testing the equipment to be purchased. Other demands stretched across a wide range of technologies and unfortunately also locations across the college, the committee tried to strike the best balance between all needs and decided to apply for the following package: * _New technology_: o an *automated high-throughput **m**icroscopy* system (e.g. Operetta or similar) o the high-throughput capacity, in combination with existing image processing / analysis capacity (e.g. Definiens) will satisfy the needs for both the high-content screening and to a large degree ImageStream needs * _Upgrade_: o *upgrade of the existing intravital microscope (FILM-CF3)* with options including high-sensitivity hybrid detectors, 405 laser, a cabinet for mouse handling, laser dispersion correction (detailed options to be checked with manufacturers) * _Support_: o *one full-time FILM staff post for Hammersmith* for the duration of the grant * _Replacement_: o *a high-sensitivity new-generation confocal* (similar to the Zeiss LSM-780 in Hammersmith), to be located in SAFB / South Kensington * _Possible options for small, complementary equipment add-ons_: o FleXcell Tensions System o ONIX microfluidic platform (if not already funded by other sources through other sources) o a high-precision multi-channel peristaltic pump system Obviously, we can't apply for everything on the list at the same time, some things will have to wait for the next equipment grant call. We now need to finalise the application - and for that *we need your support*: Those of you willing to support the application with your scientific interest and track record, please do get back to us soon - the deadline for the preliminary application is 2nd of December. And although the major bits of equipment to go on the application have been decided, if there are smaller add-ons missing on the list that would help with your microscopy in FILM, please let us know. Good imaging, Martin _*Information: *_ * http://www.wellcome.ac.uk/Funding/Biomedical-science/Funding-schemes/Strate… _*Summary of **m**icroscopy needs in Imperial College - FILM, feedback 2013*:_

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[FILM-Users 00482] Fwd: FW: Upcoming Meeting - American Society for Photobiology - 37th Meeting
by Martin Spitaler 28 Feb '14

28 Feb '14

...another one forwarded for your information - note the plenary lecture by Roger Tsien, Nobel Laureate for his contribution to the discovery and development of GFP, Martin -------- Original Message -------- Subject: FW: Upcoming Meeting - American Society for Photobiology - 37th Meeting Date: Thu, 27 Feb 2014 19:51:36 +0000 From: Kuimova, Marina K <m.kuimova(a)imperial.ac.uk> To: Tortelli, Maria A <m.tortelli(a)imperial.ac.uk>, Spitaler, Martin <m.spitaler(a)imperial.ac.uk> CC: 'Tayyaba.asp' (tayyaba.asp(a)gmail.com) <tayyaba.asp(a)gmail.com> Hi Maria and Martin Can you please circulate the flyer below to the department and to the Facility user list? (I've been asked to publisize as widely as possible) Many thanks! Marina Inline image 1 Thanks for your time and your help, and I look forward to seeing you at ASP 2014. Kind Regards, Tayyaba Hasan, Ph.D. Chair, ASP 2014 Biennial Meeting, Professor of Dermatology and of Health Sciences and Technology Wellman Center for Photomedicine, Harvard Medical School Massachusetts General Hospital 40 Blossom Street, (Bartlett 314), Boston, MA 02114 http://www.dartmouth.edu/~pdt/ <http://www.dartmouth.edu/%7Epdt/> http://www2.massgeneral.org/wellman/faculty-hasan-pi.htm http://hst.mit.edu/users/thasanpartnersorg Phone: 617-726-6996 FAX: 617-724-1345 Administrative Assistant:Bernadette Vijayakanthan Email: bvijayakanthan(a)partners.org <mailto:bvijayakanthan@partners.org> Phone: 617-726-6856

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[FILM-Users 00481] Fwd: [CISBIC-RA] [cisbio] CISBIO seminar: Thu March 6, G47A Flowers, 4pm
by Martin Spitaler 28 Feb '14

28 Feb '14

...forwarded for your information - should contain lots of microscopy and interesting image analysis, Martin -------- Original Message -------- Subject: [CISBIC-RA] [cisbio] CISBIO seminar: Thu March 6, G47A Flowers, 4pm Date: Fri, 28 Feb 2014 15:55:02 +0000 From: Endres, Robert G <r.endres(a)imperial.ac.uk> To: cisbio <cisbio(a)imperial.ac.uk> CC: Bioinformatics <fons-pgt-bioinf-shpnt(a)imperial.ac.uk>, "c.j.weijer(a)dundee.ac.uk" <c.j.weijer(a)dundee.ac.uk>, "Nurboja, Sandrine A C" <s.nurboja(a)imperial.ac.uk> Dear All, Prof. Kees Weijer from Dundee University will give next week's CISBIO seminar, entitled "Collective cell migration in development and morphogenesis". G47A Flowers, Thursday March 6, from 4-5pm For further information, see http://www.lifesci.dundee.ac.uk/people/kees-weijer Refreshments will be served afterwards. Looking forward to seeing you all! Best wishes, Robert ---------------------------------------------------------------- Dr. Robert Endres Reader in Systems Biology Imperial College London Tel: +44 (0)20 7594 9537 r.endres(a)imperial.ac.uk Personal: http://www.imperial.ac.uk/people/r.endres Group: http://www3.imperial.ac.uk/biologicalphysics

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[FILM-Users 00480] Fwd: [lsr-group] Talk at IC
by Martin Spitaler 27 Feb '14

27 Feb '14

...forwarded for your information, Martin -------- Original Message -------- Subject: [lsr-group] Talk at IC Date: Thu, 27 Feb 2014 12:42:08 +0000 From: Owen, Dylan <dylan.owen(a)kcl.ac.uk> To: 'lsr-group(a)imperial.ac.uk' <lsr-group(a)imperial.ac.uk> See below for details of Christian Eggeling's STED talk at IC next month... Dear All The next Chemical Physics seminar will be given by, Dr Christian Eggeling, Weatherall Institute of Molecular Medicine, University of Oxford. To be held at 12 noon, Thursday 27^th March '14, Pippard Lecture Theatre, Sherfield Building. *"Investigation of nanoscale membrane dynamics using super-resolution STED microscopy"* *Abstract* ** Lipid-lipid and lipid-protein plasma membrane interactions such as the formation of lipid nanodomains (often denoted "rafts") or restrictions of molecular plasma-membrane diffusion by cortical cytoskeleton compartments are considered to play a functional part in a whole range of membrane-associated processes. However, the direct and non-invasive observation of such structures in living cells is impeded by the resolution limit of >200nm of a conventional far-field optical microscope. With the superior spatial resolution of STED nanoscopy with effective focal spot sizes down to 20-30 nm in living cells, it is now possible to directly resolve such membrane heterogeneities, e.g., by imaging and investigating protein nanoclusters on the membrane surface [1]. On the other hand, the combination of STED nanoscopy with tools such as fluorescence correlation spectroscopy (FCS) allows the disclosure of complex nanoscopic dynamical processes. By performing FCS measurements in focal spots tuned to a diameter of down to 30 nm, we have obtained new details of molecular membrane dynamics. Unlike fluorescent phosphoglycerolipids, fluorescent sphingolipids or a number of proteins are transiently (~ 10 ms) trapped on the nanoscale in often cholesterol-mediated molecular complexes [2]. These interactions are distinct for different lipids and proteins and may play an important role in cellular functionality [3]. Comparison of these trapping characteristics to the organization and dynamics of the different fluorescent lipid analogues in model membranes reveals details of the role of lipid "rafts" [4]. On the other hand, the comparison of lipid and protein dynamics in different cells reveals different diffusion modes, mainly depending on the organization of the underlying (actin) cytoskeleton. Further, improved insights into membranes heterogeneities are realized by recent technological developments of the STED-FCS approach [5]. The novel observations shed new light on the role of lipid-protein interactions and nanodomains for membrane bioactivity in a whole gamut of cellular processes. Examples will be given for molecular processes of immune cells following infection. [1] G Donnert... PNAS (2006), 103, 11440; J Sieber... Science (2007), 317, 1072 [2] C Eggeling... Nature (2009), 457, 1159; C Ringemann... New J Physics (2009) 11, 103054; G Vicidomini... Nat Meth (2011) 8, 571 [3] V Mueller... Biophys J (2011) 101, 1651 [4] E. Szegin ... BBA Membr (2012) 1818, 1777; A. Honigmann... Fraday Disc. (2012) doi:10.1039/C2FD20107K [5] G Vicidomini ... Nat. Meth. (2011) 8, 571; A Honigmann ... Laser Focus World (2012) 48, 75; V Mueller ... Biophotonics (2012) 19, 30. *All Welcome*

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[FILM-Users 00476] FILM Club
by Martin Spitaler 26 Feb '14

26 Feb '14

Dear microscopists, next week's FILM Club we will take the opportunity to introduce the new FILM Crew member Debora Keller to you. As already mentioned, Debora's background is super-resolution microscopy, which will exactly be her main focus here in FILM. She has dedicated her PhD (EPFL Lausanne, Switzerland) and postdoc MRC Mill Hill, North London, to the optimisation of STORM and its application to investigate centriole formation and SHH signalling /(paper in press: "Unravelling the mechanisms of centriole formation in human cells using advanced microscopy techniques", JCB March 2014)/. She will also give an overview of the specifications of the new FILM super-resolution microscope (FILM SR1, a Zeiss Elyra PS1 with SIM and PALM/STORM with dual colour option) to be installed in early April. If you are interested in super-resolution microscopy, don't miss out. FILM Club Wednesday _26 Feb 2014, 4.30pm, SAFB-122_, South Kensington:* **Debora Keller (FILM): **/"Unravelling the mechanisms of centriole formation in human cells using advanced microscopy techniques"/** * See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00475] FILM Crew changes: Mark leaving, Debora coming
by Martin Spitaler 24 Feb '14

24 Feb '14

Dear microscopists, we are in the middle of two major changes to the FILM Crew: After almost four years of great services to the facility and users, *Mark Scott* will move on to seek new challenges and explore microscopy down under at the *WEHI in Melbourne*. He has been an essential part of FILM with his strong knowledge, especially in intravital two-photon microscopy, and has been central to become so successful, sharing and developing his expertise with Cristina Lo Celso's lab. Many of you will have appreciated his friendly personality and competent teaching, we thank him for all his contributions and for the good times we have shared, and wish him all the best for his new role! He will dedicate much of his time to intra-vital imaging there as well, so if his knowledge is too sorely missed, I'm sure we'll be in touch and tap into it in the future. By lucky coincidence, our*latest FILM Crew member Debora Keller *has just started here end of last week. Debora's main focus will be the new super-resolution microscope, helping with installation, testing, user training, data handling, data processing and everything that comes with it. She has spent her whole PhD at EPFL Lausanne and much of her postdoc time at the MRC in Mill Hill on super-resolution, so she will be able to give in-depth support to all super-resolution projects. But apart from that, she will also be able to help out with routine questions and trouble shooting on other FILM microscopes, it will just take a bit of time for her to get to know the large range of our equipment. For those of you who would like to say a personal goodbye to Mark or welcome to Debora, we will convene for drinks on Monday night (24th of February) from 6.30pm at the Drayton Arms <http://www.thedraytonarmssw5.co.uk/>, 153 Old Brompton Road, London SW5 0LJ. Thanks and best wishes for the future Mark, welcome and also best wishes for here Debbi, and good imaging everybody, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00474] Fwd: FW: Join us for Imperial Fringe: The Arts Experiment | Thursday 20 February, 17:00-20:00
by Martin Spitaler 18 Feb '14

18 Feb '14

...forwarded for your information - this time featuring award-winning images from FILM users... Martin -------- Original Message -------- Subject: FW: Join us for Imperial Fringe: The Arts Experiment | Thursday 20 February, 17:00-20:00 Date: Mon, 17 Feb 2014 10:35:51 +0000 From: Hay, Matilda <matilda.hay13(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi again Martin, Here is the e-flyer for our event on Thursday if you or any of the other researchers / photographers fancy dropping by and seeing their images in our pop-up gallery. Matilda cid:image001.jpg@01CDD2F2.65B60A40 <http://www.imperial.ac.uk/festival/fringe> The Arts Experiment 2.0 Science and art collide as Imperial's researchers and student societies team up their talents with ArtsFest to show that good science and creativity go hand in hand. Stimulate your senses with an evening of hands-on collaborations involving dancing, medicine, music and physics. The Imperial Fringe is a series of monthly evening public events exploring the unexpected side of science. Events are free and open to all. Prepare to think in new dimensions as you join us on a voyage of discovery and debate, inspired by Imperial’s research. *cid:image002.jpg@01CDD2F2.65B60A40*** *Date*: Thursday 20 February 2014 *Time*: 17.00 – 20.00 *cid:image003.jpg@01CDD2F2.65B60A40*** *Venue*: Main Entrance, Exhibition Road, Imperial College London, South Kensington Campus, London, SW7 2AZ (between buildings 17 and 28 on the map <http://workspace.imperial.ac.uk/campusinfo/public/sthkencampus.pdf>). cid:image004.jpg@01CDD2F2.65B60A40 *Join us from**17.00 – 20.00 **for***hands-on demos, activities and discussion in the College Main Entrance. Drop in at any time. Activities include: * Listen to beats provided by the Jazz & Rock Society and find out which music sets your pulse racing with researchers from theImperial Centre for Experimental and Translational Medicine. <http://www1.imperial.ac.uk/departmentofmedicine/divisions/experimentalmedic…> * Marvel at the beauty in science and enjoy art inspired by surgery in our Art Exhibition. With images from theFaculty of Imaging and Light Microscopy <http://www3.imperial.ac.uk/imagingfacility>. * How does improvised vs. practiced music change our brains? Which do we prefer and why? JoinProfessor Henrik Jensen <http://www2.imperial.ac.uk/%7Ehjjens/>, Dept of Mathematics and the Imperial College String Ensemble in the College Café to find out. *Three live performances at 6.30, 7.00 and 7.30 first come, first served* * Sometimes our ears deceive us. Listen to mysterious phantom sounds and try to create them yourself using a synthesizer. With Music Technology Society andDr Tobias Reichenbach <http://www.imperial.ac.uk/AP/faces/pages/read/Home.jsp?person=reichenbach&_…>, Dept of Bioengineering. * Tie yourself in knots as the Juggling Society andProfessor Kevin Buzzard <http://www.imperial.ac.uk/AP/faces/pages/read/Home.jsp?person=k.buzzard&_ad…>, Dept of Mathematics, unveil the mathematics behind juggling. * Get messy, and bring out the medical illustrator in you. Draw on your right or left brains, and use medical props to stretch your artistic side with LeoSoc. * Just how much pressure do dancers put on their feet? Measure the forces involved with Dr Tom Burton, MSk Lab <http://www1.imperial.ac.uk/msklab/aboutus/>, and Dance Company. * Get creative with Artifact, a collaboration between students at Imperial and the RCA. * From piccolo to tuba, what makes wind instruments work, and why do they differ in sound? Take a tuneful tour with Imperial College wind Band. * A pay bar will be open throughout *RSVP:*Please let us know you are coming on Facebook <https://www.facebook.com/events/659863947409765>or by emailing festival(a)imperial.ac.uk <mailto:festival@imperial.ac.uk> cid:image005.gif@01CDD2F2.65B60A40 cid:image006.gif@01CDD2F2.65B60A40*Contact |* <mailto:events@imperial.ac.uk> cid:image006.gif@01CDD2F2.65B60A40 *Campus Map |* <http://www3.imperial.ac.uk/campusinfo> cid:image006.gif@01CDD2F2.65B60A40 *Coming to an Event * <http://www3.imperial.ac.uk/events/comingtoanevent> Stay informed with more Imperial College London News and Events: cid:image006.gif@01CDD2F2.65B60A40 Events e-Bulletin | <http://www3.imperial.ac.uk/communications/bulletin> cid:image006.gif@01CDD2F2.65B60A40 News e-Bulletin | <http://www3.imperial.ac.uk/media/dailynewsdigest> cid:image006.gif@01CDD2F2.65B60A40 RSS Feeds | <http://www3.imperial.ac.uk/college/aboutthissite/rss> cid:image006.gif@01CDD2F2.65B60A40 Online Lectures | <http://www3.imperial.ac.uk/college/media/onlinelectures> cid:image006.gif@01CDD2F2.65B60A40 Monthly Podcast | <http://www3.imperial.ac.uk/college/media/podcasts> cid:image006.gif@01CDD2F2.65B60A40 Reporter | <http://www3.imperial.ac.uk/reporter> cid:image006.gif@01CDD2F2.65B60A40 Imperial Twitter <http://twitter.com/imperialcollege>

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[FILM-Users 00473] Fwd: VEGFR-2 staining
by Martin Spitaler 18 Feb '14

18 Feb '14

...forwarded request: Can anybody help? Best, Martin -------- Original Message -------- Subject: VEGFR-2 staining Date: Fri, 14 Feb 2014 12:50:30 +0000 From: Reina Torres, Ester <e.reina-torres12(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, We are trying to stain mouse eyes for Vascular Endothelial Growth Factor Receptor 2 and we have problems with the Ab. The one we have needs antigen retrieval and it seems to damage our delicate samples. I was wondering if you could forward this email to the FILM users in order to ask for advice in this matter. If anyone knows a good Ab anti-VEGFR-2 for mice we would appreciate the information. Thanks, Ester ……………………………………….. Ester Reina-Torres - PhD Student Department of Bioengineering Imperial College London South Kensington Campus London SW7 2AZ

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[FILM-Users 00472] Fwd: Fwd: POSTDOC AND MICROSCOPIST POSITIONS AT BIRMINGHAM
by Martin Spitaler 12 Feb '14

12 Feb '14

...forwarded for your information... Martin -------- Original Message -------- Subject: Fwd: POSTDOC AND MICROSCOPIST POSITIONS AT BIRMINGHAM Date: Wed, 12 Feb 2014 16:36:17 +0000 From: Alex Sossick <ajs17(a)CAM.AC.UK> Reply-To: Alex Sossick <ajs17(a)CAM.AC.UK> To: UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK <UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK> > > Dear Friends and Colleagues, > > > I would be very grateful if you could put up the attached posters on your corridor walls, and/or forward this message to anyone who you think might be interested. We are recruiting for two posts: > > > 1. POST-DOCTORAL RESEARCH FELLOW in glia and nervous system regeneration in Drosophila. > > Please visit: http://www.biosciences-labs.bham.ac.uk/hidalgo/Alicia_Hidalgo_Lab_Home.html > > And apply at: https://atsv7.wcn.co.uk/search_engine/jobs.cgi post reference : 47734 > > > > 2. MICROSCOPIST EXPERIMENTAL OFFICER for BIOSCIENCES ADVANCED LIGHT MICROSCOPY FACILITY: ideally for someone who has just finished the PhD using extensively confocal microscopy. > > Please visit: http://www.birmingham.ac.uk/facilities/balm/index.aspx <http://www.birmingham.ac.uk/facilities/balm/index.aspx> > > And apply at: http://www.birmingham.ac.uk/staff/jobs/index.aspx post reference 47717: > > > > Application deadline for both 27th February 2014. > > > Thank you so much!! > > Best wishes, > > Alicia > -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT -- > > > -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT -- > > > > Dr Alicia Hidalgo > NeuroDevelopment Group > School of Biosciences > University of Birmingham > Edgbaston > Birmingham B15 2TT > United Kingdom > > Phone: 00 44 (0)121 4145416 (office) > +44 (0)121 4143392 (lab) > skype: hidalgoa.1 > Fax: +44 (0)121 4145445 > www.biosciences-labs.bham.ac.uk/hidalgo <http://www.biosciences-labs.bham.ac.uk/hidalgo> > > -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT --

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[FILM-Users 00471] Fwd: ESRIC Summer School
by Martin Spitaler 12 Feb '14

12 Feb '14

...forwarded for your information... Martin -------- Original Message -------- Subject: ESRIC Summer School Date: Wed, 12 Feb 2014 11:51:06 +0000 From: Alison Dun <manager(a)esric.org> To: Undisclosed recipients:; Dear All, Please pass this across your institute or to anyone you think may be interested, ESRIC is hosting its inaugural Super-Resolution Summer School from the 4th - 8th of August this year and registration is now open. This 5-day residential course, held in Edinburgh, will cover the theory, development and application of all super-resolution technologies through informal seminars from leading academics in the field. In addition we have hands-on workshops from all of the major microscope suppliers on super-resolution systems. The course also offers ample time to gain advice from academic speakers and suppliers through Q&A and social events. To apply please visit the Olympus website http://www.olympus.co.uk/medical/en/medical_systems/training_events/event_d… download the Events Brochure and send your application to danielle.browning(a)olympus.co.uk <mailto:danielle.browning@olympus.co.uk> Looking forward to seeing you in August, Thanks, Ali Dr. Alison Dun Technology and Facility Manager Edinburgh Super Resolution Imaging Consortium (ESRIC) Heriot-Watt University Edinburgh EH14 4AS Tel: +44 7977 518 581 http://www.esric.org <http://www.esric.org/> Dr. Alison Dun Technology and Facility Manager Edinburgh Super Resolution Imaging Consortium (ESRIC) Heriot-Watt University Edinburgh EH14 4AS Tel: +44 7977 518 581 http://www.esric.org

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