August 2013 - FILM-Users - imperial.ac.uk

[FILM-Users 00347] Definiens training course
by Martin Spitaler 30 Sep '13

30 Sep '13

Dear microscopists, I'm very pleased to let you know that we have recently been successful with an application for the College's "equipment sharing grant", and part of of the money will pay towards two training courses for the powerful Definiens Developer XD software, one for beginners and one for advanced users. This will mean that both training courses will be available at a subsidised rate of probably ~£200-300 per participants (rates still need to be finalised, also dependent on the number of participants). Many of you have already used the Definiens software for a range of challenging image analysis tasks, from finding vesicles in noisy confocal images to identifying cells in 3D datasets, multi-parameter analysis of muscle fibres to immunological synapses. The power of the software lies in the easy access to a large range of professional image analysis and computer vision tools - with full control of all parameters - and in a dedicated architecture for large-scale batch processing (the largest dataset so far consisted of >30,000 images). It also contains tools for cell tracking, fuzzy logics and machine learning, which might be covered in the advanced course - this will depend on user demand. Please let me know if you are interested in attending either of the two training courses. And if you are not sure - if there is enough interest to learn more about the software, we could use the May FILM Club (31st of May) to give you an overview, based on projects in FILM. Good imaging, Martin PS: other improvements covered by the Equipment Sharing Grant are upgrades to our Widefield 2 microscope (64bit computer to deal with larger data files for live imaging, RGB camera for histological sections) and a new data processing server, which will allow processing of much larger datasets -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00402] new RGB camera and computer on WF2
by Martin Spitaler 13 Aug '13

13 Aug '13

Dear microscopists, with support from the College's Equipment Sharing Grant we now finally have an RGB camera for histology imaging on Widefield 2 (South Kensington). It has a nixe, large sensor with 2452 x 2056 pixels, 3x12bit digitisation. Thanks to this grant, we also have upgraded to a new 64-bit Win-7 computer, so we can now run the newest version of the Hamamatsu HCImage software, which allows automated tiling and stitching of large areas. In combination with the new camera, this will finally allow efficient acquisition of large histology slides. If this is what you need, please get in touch. For those located in Hammersmith: HWF1 has had this functionality already. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00401] Fwd: H2BTagRFP
by Martin Spitaler 12 Aug '13

12 Aug '13

...can anybody help Nicola? Best regards, Martin -------- Original Message -------- Subject: H2BTagRFP Date: Mon, 12 Aug 2013 12:36:54 +0100 From: Ruivo, Nicola R <n.ruivo(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin Do you know of any group that might have or is using H2BTagRFP for imagining? We're in search a donation of some DNA if anyone would be willing to help us out. Kind regards, Nicola *Nicola Ruivo (M.Sc.)* *Lo Celso Laboratory* *Senior Research Technician * *Imperial College London* *Cell & Molecular Biology* *Sir Alexander Fleming Building* *South Kensington Campus* *SW7 2AZ* *email: n.ruivo(a)imperial.ac.uk <mailto:n.ruivo@imperial.ac.uk>* *Telephone: 020 7594 2466 * 108102810593603702

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[FILM-Users 00400] Fwd: Super resolution microscopy post doc post.
by Martin Spitaler 09 Aug '13

09 Aug '13

...forwarded for your information, Martin -------- Original Message -------- Subject: Super resolution microscopy post doc post. Date: Fri, 9 Aug 2013 15:24:07 +0100 From: Nick Barry <nbarry(a)MRC-LMB.CAM.AC.UK> Reply-To: Nick Barry <nbarry(a)MRC-LMB.CAM.AC.UK> To: <UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK> Dear All I am currently advertising for a post doc to work on super resolution imaging . The sucessful candidate would be awarded an MRC Career Development Fellowship. If you know of a suitable candidate please pass on the link http://www.nature.com/naturejobs/science/jobs/334781-postdoctoral-scientist Many thanks Nick Barry MRC Laboratory of Molecular Biology -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT --

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[FILM-Users 00399] Fwd: Fw: AstraZeneca Recruitment
by Martin Spitaler 05 Aug '13

05 Aug '13

...forwarded for your information, could be an interesting job, especially if you have done a bit of high-throughput and image analysis - (AstraZeneca are intensive users of Definiens), Martin -------- Original Message -------- Subject: Fw: AstraZeneca Recruitment Date: Mon, 5 Aug 2013 10:35:42 +0100 From: Joanne Marrison <joanne.marrison(a)YORK.AC.UK> Reply-To: Joanne Marrison <joanne.marrison(a)YORK.AC.UK> To: <UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK> Hi All, Please see below for recruitment opportunities at AstraZeneca, closing date 16th August Best wishes Jo -- Joanne Marrison Imaging and Cytometry Laboratory (B/K051) Bioscience Technology Facility, Department of Biology University of York, York YO10 5DD Tel: +44(0) 1904 328720 www.york.ac.uk/biology/tf <http://www.york.ac.uk/biology/tf> EMAIL DISCLAIMER http://www.york.ac.uk/docs/disclaimer/email. <http://www.york.ac.uk/docs/disclaimer/email.htm> We are recruiting senior scientists into our High Content Biology group at AstraZeneca. If you know of anyone who might be interested please feel free to share the advert. http://gs.globalsuccessor.com/fe/tpl_astrazenecaV2.asp?s=4A515F4E5A565B1A&j… Best wishes Bev *Bev Isherwood* Team Leader ___________________________________________ *AstraZeneca* R&D | Innovative Medicines | Discovery Sciences 14F3 Mereside, Alderley Park, Macclesfield, Cheshire, SK10 4TG Tel: +44 (0)1625 513109 <tel:%2B44%20%280%291625%20513109> (Extension 23109) _beverley.isherwood(a)astrazeneca.com <mailto:beverley.isherwood@astrazeneca.com>_ __ htm <http://www.york.ac.uk/docs/disclaimer/email.htm> -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT --

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[FILM-Users 00398] Fwd: FW: EUBIAS2013: European BioImage Analysis Symposium in Barcelona, Oct. 7- 8th
by Martin Spitaler 02 Aug '13

02 Aug '13

...forwarded for your information, Martin -------- Original Message -------- Subject: FW: EUBIAS2013: European BioImage Analysis Symposium in Barcelona, Oct. 7- 8th Date: Fri, 2 Aug 2013 13:03:48 +0200 From: Stefan Terjung <terjung(a)embl.de> Reply-To: <terjung(a)embl.de> Organisation: EMBL To: <elmi(a)embl.de> *From:*Eubias2013 Organization [mailto:eubias2013@gmail.com] *Sent:* Freitag, 2. August 2013 03:34 *To:* stefan.terjung(a)embl.de; elmi(a)embl.de *Subject:* EUBIAS2013: European BioImage Analysis Symposium in Barcelona, Oct. 7- 8th Dear ELMI members, We will organize a quite unique type of bioimage analysis meeting in Barcelona, from 7th to 12th October this year. The meeting aims at strengthening the image analysis community in Europe and at increasing the accessibility of image analysis tools for the community and end-users. The event features ·An Open Community Meeting, (Oct. 7-8th, registration opened now), with "Software Showcase" presentations contributed by developers, open discussions and debates, and oral and posters contributions, participation open to everyone. ·A "Taggathon" (on invitation, Oct. 9-11th) to create an interactive webtool for the community, and to be discussed altogether previously at the meeting. ·A BioImage Analysis Course (BIAS 2, Oct. 9-12th) for imaging facility staffs, which includes participation to the Meeting. We welcome you to sign up to the BioImage Community Meeting on October 7th and 8th. Please visit the website below for more details and for registration: http://eubias2013.irbbarcelona.org We invite members of European Imaging facilities to apply now to the BIAS 2 course on Image Analysis and scientific programming (same link). We also ask you to please forward this email to your national imaging communities and co-workers. This event was conceptualized at the last ELMI meeting 2013 in Arcachon, is mainly sponsored by*EuroBioImaging *(www.eurobioimaging.org <http://www.eurobioimaging.org>) and *OME*, and by companies bridging open software (e.g. Bitplane). EuBIAS2013 is open to further sponsor contributions and participation from the private sector. EuBIAS2013 is hosted by IRB Barcelona and is organized by many people from the University of Dundee, EMBL (Heidelberg), IRB Barcelona, CRG (Barcelona), EPFL (Lausanne), ETH (Zuerich), DZNE (Bonn), Institut Curie (Paris). If BioImage Analysis is key to your research, do not miss this unique event to commit yourself in boosting the accessibility to BioImage Analysis tools and strengthening the community. Sincerely, Kota Miura, Centre for Molecular and Cellular Imaging, EMBL Heidelberg Jason Swedlow, Univ. of Dundee, Data Management and Analysis Work Package, EuroBioImaging Julien Colombelli & Sébastien Tosi, Advanced Digital Microscopy, IRB Barcelona To contact us: eubias2013(a)gmail.com <mailto:eubias2013@gmail.com> -- European BioImage Analysis Symposium - Barcelona 7 to12th of October http://eubias2013.irbbarcelona.org Organization Committee: Julien Colombelli - IRB Barcelona - julien.colombelli(a)irbbarcelona.org <mailto:julien.colombelli@irbbarcelona.org> (tel: +34 93 4020451) Sébastien Tosi - IRB Barcelona - sebastien.tosi(a)irbbarcelona.org <mailto:sebastien.tosi@irbbarcelona.org> Kota Miura - EMBL - kota.miura(a)embl.de <mailto:kota.miura@embl.de> Jason Swedlow - Univ of Dundee, Data Management and Analysis WP, EuBI

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