April 2013 - FILM-Users - imperial.ac.uk

[FILM-Users 00343] Fwd: in situ transcript hybridisation
by Martin Spitaler 24 Apr '13

24 Apr '13

...see the forwarded question by Tony Nolan below - can anyone help? Martin -------- Original Message -------- Subject: in situ transcript hybridisation Date: Wed, 24 Apr 2013 10:38:25 +0100 From: Nolan, Tony F J <t.nolan(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin, by any chance do you know of any groups that do in situ transcript localisation in tissues? I ask because we have a PhD student who is planning to look at localisation of transcripts in the mosquito ovary and testis but none of us have ever done it before. If there was someone in College who had this type of experience then it would be great as a point of reference. If it were Drosophila experience then all the better but experience in any animal tissue would be great. Thanks Tony

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[FILM-Users 00344] COMMERCIAL Fwd: New ibidi Adenovirus Cloning, Purification, and Transduction Products – Order Your Free Sample Today!
by Martin Spitaler 24 Apr '13

24 Apr '13

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[FILM-Users 00342] Fwd: our new imaging paper
by Martin Spitaler 24 Apr '13

24 Apr '13

...forwarded for your information, some quite spectacular movies of B cell killing, all done on our FILM Confocal 2, while Dan Davis was still here at Imperial College. Congratulations, Dominika and Dan, Martin -------- Original Message -------- Subject: our new imaging paper Date: Wed, 24 Apr 2013 13:59:35 +0100 From: Davis, Daniel M <d.davis(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin, For your interest, Dominika's paper has just been published (FILM and you are acknowledged) and a 'press release' on this is here: http://www.manchester.ac.uk/aboutus/news/display/?id=9906 ------------------------------------------------------------------------ *From:* film-users-bounces(a)imperial.ac.uk [film-users-bounces(a)imperial.ac.uk] on behalf of Martin Spitaler [m.spitaler(a)imperial.ac.uk] *Sent:* 18 April 2013 19:14 *To:* film-users *Subject:* [FILM-Users 00337] Fwd: Summer workshop ...forwarded for your information, Martin -------- Original Message -------- Subject: Summer workshop Date: Tue, 16 Apr 2013 10:25:54 +0000 From: Paul Thomas (BIO) <P.Thomas(a)UEA.AC.UK> Reply-To: Paul Thomas (BIO) <P.Thomas(a)UEA.AC.UK> To: <UK-MICROSCOPY(a)JISCMAIL.AC.UK> Hi everyone, I’m running my live-cell imaging workshop again this June (poster attached). I would be very grateful if you could bring it to the attention of anyone you think might be interested: *Live-Cell Imaging* */University of East Anglia, Norwich, UK/* Organised by Dr Paul Thomas (University of East Anglia) This three-day workshop provides theoretical and practical guidance on imaging live cells and tissues. Focusing on the use of fluorescence microscopy, the course is designed for beginners. Training includes lectures and hands-on experience of both short- and long-term observations using widefield, confocal, and multi-photon microscopy. The final day includes demonstrations of image analysis for object identification, quantification, tracking, and fluorescence lifetimes. Space is limited to 12 trainees. The workshop will be held from Tues, June 11^th to Thurs, June 13^th 2013 in the Henry Wellcome Laboratory for Cell Imaging, School of Biological Sciences. Applications should be submitted using the Physiological Society web-page (http://www.physoc.org/live-cell-imaging-11-13-june-2013-tbc) by 31^st May 2013. (Members wanting to apply for travel grants should apply before 30^th April 2013.) Many thanks, Paul. ____________________ Dr. Paul Thomas, Director, The Henry Wellcome Laboratory for Cell Imaging, School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, United Kingdom. e-mail: p.thomas(a)uea.ac.uk Tel: +44-1603-592196 Fax: +44-1603-592250 Imaging web-site: http://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome… Personal web-page: http://biobis.bio.uea.ac.uk/biosql/fac_show.aspx?ID=548 ------------------------------------------------------------------------ To unsubscribe from the UK-MICROSCOPY list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=UK-MICROSCOPY&A=1

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[FILM-Users 00341] Fwd: RMS Course: Cell Imaging Techniques, 01 - 06 September 2013
by Martin Spitaler 24 Apr '13

24 Apr '13

...forwarded for your information; and don't forget that this and many more events are also on the FILM website under LINKS / MEETINGS, COURSES, COMPETITIONS <http://www3.imperial.ac.uk/imagingfacility/links/events>, Martin -------- Original Message -------- Subject: RMS Course: Cell Imaging Techniques, 01 - 06 September 2013 Date: Wed, 24 Apr 2013 07:26:20 +0000 From: Royal Microscopical Society <reply(a)rmsnewsletter.org.uk> Reply-To: Royal Microscopical Society <reply(a)rmsnewsletter.org.uk> To: <m.spitaler(a)imperial.ac.uk> CELL IMAGING TECHNIQUES COURSE: Oxford Brookes University, 01 - 06 September 2013. This Royal Microscopical Society Course will be of immense value to any life scientist or cell biologist from a research or technical background who is working in a hospital, research institute, industry or academia. It is suitable for anyone who wishes to improve their knowledge of current cell imaging techniques, either for routine purposes or for research. No previous knowledge is assumed, and it will be of value to complete novices and those with previous experience. It covers a wide range of applications in pathology, cell biology and the plant sciences. The course has been running for more than a decade and is updated each year to take into account new developments in cell imaging approaches and closely related technologies. It is structured towards a technical understanding of techniques, as once these are mastered they can be applied to almost any cell / tissue system or research project. For further details, including online registration, visit - http://www.rms.org.uk/events/Forthcoming_Events/CellImagingTech Registration fees - RMS/EMS member rate £1050 Standard Rate £1250 Accommodation package: 5 nights £250 Scientific Organisers: Susan Brooks, John Runions and Pippa Hawes. ============================================== You are receiving this email because you are a valued contact of the Royal Microscopical Society. If you do not wish to receive further correspondence, use the unsubscribe facility below. Unsubscribe m.spitaler(a)imperial.ac.uk from this list: http://rms.us2.list-manage.com/unsubscribe?u=09d07c67a261f2ebc6e211496&id=7… Our mailing address is: Royal Microscopical Society 37/38 St Clements Street Oxford, OXON OX4 1AJ Our telephone: +44 (0)1865 254760 Forward this email to a friend: http://us2.forward-to-friend.com/forward?u=09d07c67a261f2ebc6e211496&id=893… Update your profile: http://rms.us2.list-manage1.com/profile?u=09d07c67a261f2ebc6e211496&id=736e…

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[FILM-Users 00340] FILM Club this Friday 26 April: Jill Johnson - Jill Johnson: “3D imaging of trachea whole mount tissues from normal and asthmatic mice”
by Martin Spitaler 22 Apr '13

22 Apr '13

Dear microscopists, this Friday the FILM Club is back, and this time with Jill Johnson from Sarah Rankin's lab in Leukocyte Biology: *Jill Johnson: “3D imaging of trachea whole mount tissues from normal and asthmatic mice”* Allergic asthma is an inflammatory lung disease that ultimately leads to structural changes to the airway wall and breathing difficulties. In this talk, Dr Johnson will present her work investigating the mobilisation of multiple cell types in the airway wall through the use of multicolour three-dimensional confocal imaging. Friday 26 April 2013, 1pm, seminar room SAFB-120, Fleming Building, South Kensington campus. Don't miss out, it will be interesting for both the microscopy and the biology. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00339] Fwd: [CISBIC-RA] [cisbio] FW: One-day meeting Complex and Emergent Behaviours in Biological Systems
by Martin Spitaler 19 Apr '13

19 Apr '13

...forwarded for your information; by the way, it's not specifically mentioned, but it's in Manchester, Martin -------- Original Message -------- Subject: [CISBIC-RA] [cisbio] FW: One-day meeting Complex and Emergent Behaviours in Biological Systems Date: Wed, 17 Apr 2013 15:27:07 +0100 From: Endres, Robert G <r.endres(a)imperial.ac.uk> To: cisbio <cisbio(a)imperial.ac.uk> ________________________________________ From: Tommaso Biancalani [tommaso.biancalani(a)gmail.com] Sent: 17 April 2013 15:19 Subject: Fwd: One-day meeting Complex and Emergent Behaviours in Biological Systems Would you please forward this to anyone who might be interested. Thanks, Tommaso ---------- Forwarded message ---------- From: Date: 17 April 2013 15:07 Subject: One-day meeting Complex and Emergent Behaviours in Biological Systems To: "MANCHESTER-COMPLEXITY(a)JISCMAIL.AC.UK<mailto:MANCHESTER-COMPLEXITY@JISCMAIL.AC.UK>" <MANCHESTER-COMPLEXITY(a)jiscmail.ac.uk<mailto:MANCHESTER-COMPLEXITY@jiscmail.ac.uk>> Cc: Tommaso Biancalani <tommaso.biancalani(a)gmail.com<mailto:tommaso.biancalani@gmail.com>>, George Constable <george.constable(a)postgrad.manchester.ac.uk<mailto:george.constable@postgrad.manchester.ac.uk>>, Toby Brett <tobybrett(a)gmail.com<mailto:tobybrett@gmail.com>> Dear colleague, In order to cope with many of the current challenges in biology, one must understand how collective behaviour arises from microscopic interactions. The techniques that address these questions define the field of complex systems, which is by its nature inter-disciplinary and requires joint efforts between biologists, and scientists with a more theoretical background. We are organising a meeting on complex systems and biology that will take place in Manchester on the 15th of May. The aim is to bring together those with an interest in biological questions which can be addressed with methods from physics, mathematics or computer science. The meeting is especially targeted at PhD students and post-docs. Four invited speakers will talk in the morning. Short talks by PhD students and post-docs will be given in the afternoon. PLEASE REGISTER BEFORE FRIDAY 3rd OF MAY IF YOU WISH TO ATTEND THE MEETING A LIMITED NUMBER OF SLOTS FOR SHORT TALKS IS AVAILABLE To register, send an e-mail to: tommaso.biancalani(a)postgrad.manchester.ac.uk<mailto:tommaso.biancalani@postgrad.manchester.ac.uk> Please indicate in your registration e-mail whether you wish to give a short oral presentation. Post-docs and PhD students are encouraged to apply. Complex and Emergent Behaviours in Biological Systems Wednesday 15th of May Schuster Building, Niels Bohr Common Room 6th floor 10:00 - 10:30 - Coffee and Introduction 10:30 - 11:10 - Dr. Robert Endres (Imperial College, London) "Emergence of stereotypical behaviour in chemotactic amoeba" 11:10 - 11:50 - Dr. Marco Cosentino Lagomarsino (Universite Pierre et Marie Curie, Paris) "Cell-size control in E.coli" 11:50 - 12:30 - Dr. Rosalind Allen (University of Edinburgh) "Complex dynamics in microbial ecosystems" 12:30 - 13:10 - Dr. Mauro Mobilia (University of Leeds) "Large-fluctuation effects on the fixation of a deleterious allele under mutation pressure" 13:10 - 14:30 - Lunch 14:30 - 16.30 - Short talks Organisers: Tommaso Biancalani, George Constable and Toby Brett

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[FILM-Users 00338] Fwd: Re-Entry of Small World Images
by Martin Spitaler 18 Apr '13

18 Apr '13

...forwarded for your information (deadline 30 April), Martin -------- Original Message -------- Subject: Re-Entry of Small World Images Date: Tue, 16 Apr 2013 16:14:49 -0400 From: Michael W. Davidson <davidson(a)magnet.fsu.edu> To: <m.spitaler(a)imperial.ac.uk> Dear Dr. Spitaler, I hope you will be able to enter the 2013 Nikon Small World competition this year and perhaps even the new video competition: Small World in Motion. Contestants can enter up to 3 images or videos in each competition. If you are too busy or haven't had the opportunity to gather new images, let me know and I can transfer several of your previously entered images into this year's competition. Each year the competition has several hundred high-quality images that are not selected by the judges and I would like to give these images a second chance with a new set of judges. Over the past several years, many of the images that have been re-entered have gone on to win prizes. The Small World competition URL is: http://www.microscopyu.com/smallworld/scripts/entryPage1.asp The Small World Video competition URL is: http://www.microscopyu.com/smallworld/videoregistration/entrypage1.aspx Note that everyone entering the Small World 2013 competition will receive a complimentary 2014 Small World calendar. Regards---Mike Michael W. Davidson National High Magnetic Field Laboratory 1800 E. Paul Dirac Dr. The Florida State University Tallahassee, FL 32310 Phone: 850-644-0542 Fax: 850-644-8920 Web: http://microscopy.fsu.edu

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[FILM-Users 00337] Fwd: Summer workshop
by Martin Spitaler 18 Apr '13

18 Apr '13

...forwarded for your information, Martin -------- Original Message -------- Subject: Summer workshop Date: Tue, 16 Apr 2013 10:25:54 +0000 From: Paul Thomas (BIO) <P.Thomas(a)UEA.AC.UK> Reply-To: Paul Thomas (BIO) <P.Thomas(a)UEA.AC.UK> To: <UK-MICROSCOPY(a)JISCMAIL.AC.UK> Hi everyone, I'm running my live-cell imaging workshop again this June (poster attached). I would be very grateful if you could bring it to the attention of anyone you think might be interested: *Live-Cell Imaging* */University of East Anglia, Norwich, UK/* Organised by Dr Paul Thomas (University of East Anglia) This three-day workshop provides theoretical and practical guidance on imaging live cells and tissues. Focusing on the use of fluorescence microscopy, the course is designed for beginners. Training includes lectures and hands-on experience of both short- and long-term observations using widefield, confocal, and multi-photon microscopy. The final day includes demonstrations of image analysis for object identification, quantification, tracking, and fluorescence lifetimes. Space is limited to 12 trainees. The workshop will be held from Tues, June 11^th to Thurs, June 13^th 2013 in the Henry Wellcome Laboratory for Cell Imaging, School of Biological Sciences. Applications should be submitted using the Physiological Society web-page (http://www.physoc.org/live-cell-imaging-11-13-june-2013-tbc) by 31^st May 2013. (Members wanting to apply for travel grants should apply before 30^th April 2013.) Many thanks, Paul. ____________________ Dr. Paul Thomas, Director, The Henry Wellcome Laboratory for Cell Imaging, School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, United Kingdom. e-mail: p.thomas(a)uea.ac.uk Tel: +44-1603-592196 Fax: +44-1603-592250 Imaging web-site: http://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome… Personal web-page: http://biobis.bio.uea.ac.uk/biosql/fac_show.aspx?ID=548 ------------------------------------------------------------------------ To unsubscribe from the UK-MICROSCOPY list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=UK-MICROSCOPY&A=1

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[FILM-Users 00336] Fwd: Postdoc position in microscopy - Hayday lab - London
by Martin Spitaler 15 Apr '13

15 Apr '13

...forwarded for your information, Martin -------- Original Message -------- Subject: Postdoc position in microscopy - Hayday lab - London Date: Thu, 11 Apr 2013 09:36:25 +0100 From: Francois Lassailly <Francois.Lassailly(a)CANCER.ORG.UK> Reply-To: Francois Lassailly <Francois.Lassailly(a)CANCER.ORG.UK> To: <UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK> From: Lucie Abeler-Dorner <Lucie.Abeler-Dorner(a)cancer.org.uk <mailto:Lucie.Abeler-Dorner@cancer.org.uk>> Date: Thursday, 11 April 2013 01:42 To: Francois Lassailly <Francois.Lassailly(a)cancer.org.uk <mailto:Francois.Lassailly@cancer.org.uk>> Subject: Hayday lab postdoc position Dear François, We have an immediate opening for someone with experience in microscopy and image analysis. The post is currently advertised as a three-year postdoctoral position with option of extension but is also suited for a highly qualified, highly numerate research associate. The project offers extremely exciting opportunities as part of a high-throughput immunological phenotyping programme financed by the Wellcome Trust and awarded to a multi-centre consortium led by our group at King’s College London. The consortium will over the next five years conduct a high-throughput immunological phenotyping of approximately 800-1000 knockout mouse lines generated by the Wellcome Trust Sanger Institute (please see attachment for details). If you are aware of suitable candidates, please encourage them to contact us for further information. Please feel free to forward this email to people who might be interested or who know a suitable candidate, and ideally, please let us know of any good prospects (adrian.hayday(a)kcl.ac.uk <mailto:adrian.hayday@kcl.ac.uk>; lucie.abeler-dorner(a)kcl.ac.uk <mailto:lucie.abeler-dorner@kcl.ac.uk>) Best wishes, Adrian and Lucie Adrian Hayday Kay Glendinning Professor Dept of Immunobiology King’s College London & Senior Group Leader London Research Institute, CRUK Dr Lucie Abeler-Dörner Strategic Award co-ordinator Dept of Immunobiology King’s College London NOTICE AND DISCLAIMER This e-mail (including any attachments) is intended for the above-named person(s). If you are not the intended recipient, notify the sender immediately, delete this email from your system and do not disclose or use for any purpose. We may monitor all incoming and outgoing emails in line with current legislation. We have taken steps to ensure that this email and attachments are free from any virus, but it remains your responsibility to ensure that viruses do not adversely affect you. Cancer Research UK Registered charity in England and Wales (1089464), Scotland (SC041666) and the Isle of Man (1103) A company limited by guarantee. Registered company in England and Wales (4325234) and the Isle of Man (5713F). Registered Office Address: Angel Building, 407 St John Street, London EC1V 4AD. -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT -- ------------------------------------------------------------------------ We have an immediate opening for someone with experience in microscopy and image analysis. The post is currently advertised as a three---year postdoctoral position with option of extension but is also suited for a highly qualified, highly numerate research associate. The project offers extremely exciting opportunities as part of a high---throughput immunological phenotyping programme financed by the Wellcome Trust and awarded to a multi---centre consortium led by our group at King’s College London. The consortium will over the next five years conduct a high---throughput immunological phenotyping of approximately 800---1000 knockout mouse lines generated by the Wellcome Trust Sanger Institute (see advert for details). More specifically, the project focuses on the epidermal immune compartment. As you may appreciate, this is an interesting and aesthetically beautiful composition of dendritic T cells and Langerhans cells in regularly patterned juxtaposition. Despite the growing interest in tissue---associated lymphoid and myeloid compartments, there is little understanding of what regulates their normal development, homeostasis, and spatial organization. Clearly, the mutant screen offers the opportunity to identify key new genes and pathway. We therefore require the candidate to set up and implement the confocal assessment of the mutant mice. The challenges of this job will be the development of a semi--- automated acquisition process and the application / development of high--- throughput analysis tools to extract a maximum of information from large data sets. Such developments may set an important benchmark for others to follow. Added to this, an additional incentive is the potential for a successful candidate to take---on the follow---up studies of interesting mutants, potentially developing a career development trajectory. Technical assistance will be available within the team. The job advert with the full job description can be found on the KCL website (http://www.kcl.ac.uk/depsta/pertra/vacancy/external/pers_detail.php?jobinde…) If you are aware of suitable candidates, please encourage them to contact us for further information. Please feel free to forward this email to people who might be interested or who know a suitable candidate, and ideally, please let us know of any good prospects (adrian.hayday(a)kcl.ac.uk; lucie.abeler---dorner(a)kcl.ac.uk) Best wishes, Adrian Hayday Dr Lucie Abeler---Dörner Kay Glendinning Professor Strategic Award co---ordinator Dept of Immunobiology Dept of Immunobiology King’s College London King’s College London & Senior Group Leader, London Research Institute, CRUK

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[FILM-Users 00335] COMMERCIAL Fwd: ibidi u-Plate savings from Thistle Scientific
by Martin Spitaler 15 Apr '13

15 Apr '13

...COMMERCIAL - forwarded for your information, Martin -------- Original Message -------- Subject: ibidi u-Plate savings from Thistle Scientific Date: Mon, 8 Apr 2013 16:54:36 +0100 From: James Smith <James(a)thistlescientific.co.uk> Reply-To: <enquiries(a)thistlescientific.co.uk> To: Dr Martin Spitaler <m.spitaler(a)imperial.ac.uk> Celebrating 20 Years of Biometra Thermocyclers Dear Dr Spitaler, Ibidi, in partnership with their UK Distributor, Thistle Scientific, are offering special pricing on their m-Plate range of products. ibidi are also introducing the m-Plate Angiogenesis 96 well, used primarily to investigate angiogenesis in high throughput tube formation assays. The link below will enable you to view the various products and associated pricing/discounts within this range: ibidi m-Plate offers > click here <http://www.thistlescientific.co.uk/e-news/ibidi-April-2013/ibidi-April-2013…> Should you require any further information or clairifcation, please do not hesitate to contact us. Please note that offers are valid until the 30th of June 2013 and cannot be combined with any other offers or promotions. Kind regards, *Thistle Scientific Ltd* *DFDS House, Goldie Road* *Uddingston,* *Glasgow* *G71 6NZ* *Tel: 01698 338844 Fax: 01698 338880* *email: **e* <mailto:%20enquiries@thistlescientific.co.uk>*nquiries <mailto:Enquiries@thistlescientific.co.uk>**(a)thistlescientific.co.uk* <mailto:Enquiries@thistlescientific.co.uk>

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