February 2013 - FILM-Users

[FILM-Users 00323] updated of FILM charging rates from 1 April 2013
by Martin Spitaler 26 Feb '13

26 Feb '13

Dear microscopists, based on recent developments of FILM costs and usage, we have now updated the charging rates for FILM usage to be applied from 1 April 2013. I'm pleased to see that despite the challenging financial situation of many users, we have managed to keep our broad user base, allowing moderate changes to charges. As always, the updated rates can be found on the FILM website under GUIDELINES / CHARGES / INTERNAL. <http://www3.imperial.ac.uk/imagingfacility/facility_guidelines/charges/char…> Importantly, you will see that some charges will actually go down! This reflects a simple fact: FILM does NOT make any profit, charging rates are simply the CURRENT COSTS divided by the CURRENT USAGE. Whenever usage goes up, charges go down, as simple as that. Therefore: Don't try to save money in the wrong place, the more you use our microscopes, the lower the charging rates will be (with a slight delay)!! Be assured that we very much prefer you getting more out from our microscopes for the same money. And in case you are confused by the different types of charging rates, here some explanation: The FEC RATES calculated by Research Support Services, and updated recently (see my email on 1 Feb 2013), are the estimates for FUTURE COSTS, for grants applied for now, but used in 1-5 years' time. They are updated once every year in January. The ACTUAL, CURRENT RATES are based on actual, current cost and usage data. We update them whenever usage or cost change significantly, but at least once a year (typically in April). If you need any more information, please let me know. Good imaging, Martin * Internal charging rates on the FILM website: <http://www3.imperial.ac.uk/imagingfacility/facility_guidelines/charges/char…> * Email about FEC rates: <http://mailman.ic.ac.uk/pipermail/film-users/2013/000309.html> -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00322] Fwd: TODAY: Molecular Medicine & Cell Biology Seminar Series | TODAY | 12:00-1:00pm | Room 121, SAF
by Martin Spitaler 26 Feb '13

26 Feb '13

...forwarded for your information, Martin ------------------------------------------------------------------------ Molecular Medicine & Cell Biology Seminar Series: Dr Mark Leake, University of Oxford: "Illuminating the machines of life" Tuesday 26 Feb 2013, 12.00-13.00, SAFB 121, South Kensington Host: Liming Ying -------- Original Message -------- Subject: REMINDER: Molecular Medicine & Cell Biology Seminar Series | TODAY | 12:00-1:00pm | Room 121, SAF Date: Tue, 26 Feb 2013 09:29:18 +0000 From: Moore, Peter J <peter.moore(a)imperial.ac.uk> Peter Moore Section Administrator Allergy and Clinical Immunology Section Leukocyte Biology Section & Administrator to Faculty of Medicine Campus Dean, South Kensington Campus NHLI, Imperial College Desk 124, Level 2, Sir Alexander Fleming Building London SW7 2AZ tel: 020 759 43175 email: peter.moore(a)imperial.ac.uk <mailto:peter.moore@imperial.ac.uk>

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[FILM-Users 00321] Fwd: 9th April – Photonex London – photonics technology roadshow - UCL
by Martin Spitaler 25 Feb '13

25 Feb '13

...forwarded for your information... Martin -------- Original Message -------- Subject: 9th April – Photonex London – photonics technology roadshow - UCL Date: Fri, 22 Feb 2013 12:01:29 +0000 From: Laurence Devereux/Xmark Media <news(a)xmarkmedia-mail.com> Reply-To: <ld(a)xmarkmedia.com> To: m.spitaler(a)imperial.ac.uk <m.spitaler(a)imperial.ac.uk> Photonex Roadshow eMailer Can't see images? Click here to view this e-mail in a web browser <http://news.xmarkmedia-mail.com/NLI/ViewHtmlEmail.aspx?a=431D6082DEB87D138E…> Photonex London Roadshow, Tuesday 9th April 2013, Wilkins North Cloisters, University College London Register to Attend Visiting Conference Travel Exhibitors List Dear Martin, The Photonex Roadshow will be visiting UCL on the 9th April - you are encouraged and welcome to attend. This one day event <http://news.xmarkmedia-mail.com/NLI/LE35.ashx?a=3BB6046FADC446D4E538A7E5D9F…> brings together the UK’s top photonics technology supplier companies, leading researchers and invited speakers for a special one day event. What is likely to be of interest: * There is the roadshow of 25 companies <http://news.xmarkmedia-mail.com/NLI/LE35.ashx?a=2A6F3416B71F045A4DAAB7ABC6A…> dedicated to optics, photonics and imaging technology * There is a high-level conference detailing advances in photonics tools and techniques for the life sciences * There are tutorials and educational forums being presented by companies and photonics specialists ------------------------------------------------------------------------ *Attend free educational and advanced learning opportunities:* Conference: *Advances in photonic tools and techniques for the life sciences* - see who is speaking> <http://news.xmarkmedia-mail.com/NLI/LE35.ashx?a=F2DEB557608DB4911502A7588E2…>Photonics Tutorials: *Latest photonics techniques and instrumentation* - pick one to attend> <http://news.xmarkmedia-mail.com/NLI/LE35.ashx?a=34C05FE493E915DA55456DABCE7…> ------------------------------------------------------------------------ We hope to see you at UCL. We will continue to report on the event on our web site and there will other newsletters reviewing the meetings and roadshow. And if you have any questions please call us or email our team at info(a)photonex.org <http://news.xmarkmedia-mail.com/NLI/LE35.ashx?a=83C958A867FF4A91F69BA90D022…>. Kind regards, Laurence Devereux Xmark Media Ltd, Effingham KT24 5JS, UK E: ld(a)photonex.org <http://news.xmarkmedia-mail.com/NLI/LE35.ashx?a=D4E4A95847ACD2D90DA63CCBB6B…> T: +44 (0)1372 750555 Join us on FACEBOOK <http://news.xmarkmedia-mail.com/NLI/LE35.ashx?a=E18A81DD36F672C14CEAD7B5EEC…> – ‘Like us’! LINKEDIN – Join Photonex Group <http://news.xmarkmedia-mail.com/NLI/LE35.ashx?a=1B08257C69B3114DAF2C2B39B24…> Follow us on Twitter – @photonex <http://news.xmarkmedia-mail.com/NLI/LE35.ashx?a=971A4E219AEA67F421972CF448D…>*Lunch Included for attendees who register by noon on Tuesday 2nd April (one week ahead). Event Opening Times Exhibiting Information <http://news.xmarkmedia-mail.com/NLI/LE35.ashx?a=980D8D0AD443FC1DB6EE8384688…> Join Mailing List <http://news.xmarkmedia-mail.com/NLI/LE35.ashx?a=8FE5C03E746E0C9672B5CD346B0…> Forward Email to a colleague <http://news.xmarkmedia-mail.com/newsletters/Forward.aspx?MTcyMTo5%21*%21F27…> This message is sent to Martin Spitaler at m.spitaler(a)imperial.ac.uk in good faith as we believe it likely that you will have a genuine interest in Photonex events. You may have registered this year, visited previously, enquired or we may have picked up your name whilst in conversation with a colleague. To avoid sending the wrong information we manage a large number of specific mailing lists. To update your details or change your subscription preferences, please click here <http://news.xmarkmedia-mail.com/newsletters/EditProfile.aspx?MTcyMToy%21*%2…> Old Village Hall, The Street, Effingham, KT24 5JS However, if you would rather not receive ANY future e-mails regarding our events, please visit the opt-out link by clicking here <http://news.xmarkmedia-mail.com/newsletters/ManageProfile.aspx?MTcyMTo5%21*…>

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[FILM-Users 00320] Fwd: [neuroscience-technology-network] Postdoctoral position available - two-photon targeted electrophysiology
by Martin Spitaler 22 Feb '13

22 Feb '13

...forwarded for your information, Martin -------- Original Message -------- Subject: [neuroscience-technology-network] Postdoctoral position available - two-photon targeted electrophysiology Date: Fri, 22 Feb 2013 16:50:57 +0000 From: Simon Schultz <s.schultz(a)imperial.ac.uk> Reply-To: <s.schultz(a)imperial.ac.uk> To: neuroscience-technology-network <neuroscience-technology-network(a)imperial.ac.uk> Research Associate Imperial College London -Department of Bioengineering Two-photon targeted electrophysiology From £32,100 to £34,720 per annum Fixed Term Appointment for up to 36 months Applications are invited for the above post to work with Dr Simon Schultz on a BBSRC funded project in Neural Engineering to underpin work at Imperial College on coding of sensory information in mammalian cortical circuits. This is an exciting opportunity to contribute to a BBSRC funded project in Neural Engineering with a focus on understanding neural computation. The Neural Coding Laboratory at Imperial College London is developing cutting edge optical and opto-electrophysiological technology for studying how information is encoded and processed in mammalian cortical circuits. This project will involve using a novel tool for two-photon targeted electrophysiology to obtain recordings from multiple, genetically targeted neuronal cell types simultaneously. The ultimate aim of this work is to understand the general principles underlying the information processing operations performed by the mammalian cortical circuit. Candidates must be in the possession of a doctoral degree or equivalent in neuroscience, physics, engineering or a related topic. Experience in whole-cell patch clamp electrophysiology or in vivo two-photon microscopy is a requirement. Preference will be given to candidates with a strong experimental background, and experience in quantitative neurophysiological data analysis using MATLAB. The ideal candidate would be a “tool developer” as well as “tool user”. This full-time post is available from the 1 April 2013 or as soon as possible thereafter. Informal enquiries may be addressed to Dr S Schultz, email: s.schultz(a)imperial.ac.uk. Please note that applications sent directly to this email address will not be accepted. For more information about the laboratory and research group, see http://www.schultzlab.org Our preferred method of application is online via our website at the following link: http://www3.imperial.ac.uk/employment (select “Job Search” and enter the job reference no EN20130059FH). Please complete and upload an application form as directed and submit any other relevant supporting documents such as your full CV. Closing date: 25 March 2013 _______________________________________________ neuroscience-technology-network mailing list neuroscience-technology-network(a)imperial.ac.uk https://mailman.ic.ac.uk/mailman/listinfo/neuroscience-technology-network

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[FILM-Users 00319] problem with Sharepoint alerts
by Martin Spitaler 22 Feb '13

22 Feb '13

Dear microscopists, it turns out there have been a few problems with Sharepoint Alerts to microscope messages, due to the confusing way the Sharepoint floating menu and alert setup works (it's easy to set up the alert for a specific message rather than the whole list by mistake). I've just sent out test messages for all microscopes, if you have NOT received one for any equipment you are using, please do the following: * Go to the FILM Sharepoint site * click on your name in the top-right corner and select MY SETTINGS > MY ALERTS * There is should be an entry in the style of "FILM CF1 MESSAGES" for each equipment and software you are using, without any topic * If this is not the case, please delete the wrong alerts, then go to ADD ALERT and add the missing alerts you need ONE BY ONE (it doesn't allow to add more than one at a time) Thanks, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00318] Fwd: anti CD31 clone JC/70A: Request for FILM mailing
by Martin Spitaler 19 Feb '13

19 Feb '13

...can anyone help Sietse with the request below? If so, please reply to him directly, Martin -------- Original Message -------- Subject: anti CD31 clone JC/70A: Request for FILM mailing Date: Mon, 18 Feb 2013 17:02:40 +0000 From: Braakman, Sietse <sietse.braakman09(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin, I hope all is well? I'm currently doing a small side project and we need to stain some fixed human tissue for CD31. We have had trouble finding appropriate antibodies. The epitope needs to be extracellular because we don't want to permeabilise the cells membrane. On the same tissue, one of our collaborators has successfully used the JC/70A clone (e.g. from DAKO or Abcam) but he is in Canada. By any chance, does anyone have this antibody such that we can give it a try? Many thanks, Sietse

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[FILM-Users 00317] COMMERCIAL Fwd: Test with a Free Sample: In vivo-like Cell Culture on Elastically Supported Surfaces
by Martin Spitaler 19 Feb '13

19 Feb '13

...if anyone is interested, I'll ask for a test sample, Martin -------- Original Message -------- Subject: Test with a Free Sample: In vivo-like Cell Culture on Elastically Supported Surfaces Date: Thu, 14 Feb 2013 11:50:57 +0100 From: ibidi - cells in focus <events(a)ibidi.de> To: <m.spitaler(a)imperial.ac.uk> Wird diese Nachricht nicht richtig dargestellt, klicken Sie bitte *hier* <http://13457.cleverreach.de/m/5517160/0-d4f84b553abbfd1f56abb398762087e4>. load images */In vivo/-like Cell Culture on Elastically Supported Surfaces (ESS) <http://13457.cleverreach.de/c/9936047/b683f5ae3bd6>* *Are you sure your cells grow under /in vivo/-like conditions?* Most researchers culture cells on stiff surfaces, such as glass or plastic, even though the stiffness of the cell environment has a strong influence on cell behavior /in vivo/ and /in vitro/. ibidi's ESS product line line <http://13457.cleverreach.de/c/9936048/b683f5ae3bd6> provides ready-to-use solutions for growing cells on substrates that cover a large range of physiological relevant elasticities, such as: * fat or brain (1.5 kPa) * connective tissue (15 kPa) * muscle (28 kPa) <http://13457.cleverreach.de/c/9936048/b683f5ae3bd6> * Simply fill the µ-Dish^35 mm, high ESS <http://13457.cleverreach.de/c/9936048/b683f5ae3bd6> with your cells and start your experiments under /in vivo/-like conditions!* *Applications:* * Cell culture under /in vivo/-like conditions (e.g., cardiomyocytes) * Cellular mechanics research * Immunofluorescence <http://13457.cleverreach.de/c/9936049/b683f5ae3bd6> stainings * Live cell imaging <http://13457.cleverreach.de/c/9936050/b683f5ae3bd6> of cell differentiation on soft surface * Transfection <http://13457.cleverreach.de/c/9936051/b683f5ae3bd6> * Suitable for DIC, when used with the special DIC Lid <http://13457.cleverreach.de/c/9936052/b683f5ae3bd6> /Differentiated mouse fibroblasts cultured on elastic surface (ESS 28 kPa)/ ibidi's µ-Dish^35 mm, high ESS <http://13457.cleverreach.de/c/9936048/b683f5ae3bd6> provides cell culture and high-resolution microscopy on a soft cell culture surface! *Order a FREE SAMPLE <http://13457.cleverreach.de/c/9936053/b683f5ae3bd6>* *to test ibidi's µ-Dish^35 mm, high ESS with your experiments.* Ordering Information: *Cat. No.* *Description* *Pcs./ Box* 81291 <http://13457.cleverreach.de/c/9936048/b683f5ae3bd6> µ-Dish 35 mm, high ESS, uncoated elastic surface with a stiffness of 1.5 kPa, sterile, Ø 35 mm, 2 ml volume 30 81391 <http://13457.cleverreach.de/c/9936048/b683f5ae3bd6> µ-Dish 35 mm, high ESS, uncoated elastic surface with a stiffness of 15 kPa, sterile, Ø 35 mm, 2 ml volume 30 81191 <http://13457.cleverreach.de/c/9936048/b683f5ae3bd6> µ-Dish 35 mm, high ESS, uncoated elastic surface with a stiffness of 28 kPa, sterile, Ø 35 mm, 2 ml volume 30 Wenn Sie diese E-Mail (an: m.spitaler(a)imperial.ac.uk) nicht mehr empfangen möchten, können Sie diese *hier* <http://13457.cleverreach.de/rmftlp.php?cid=0&mid=5517160&h=0-b683f5ae3bd6> abbestellen. ibidi GmbH Am Klopferspitz 19 82152 Planegg-Martinsried Deutschland www.ibidi.com events(a)ibidi.de Geschäftsführer: Dr. Roman Zantl, Dr. Valentin Kahl Handelsregister: Amtsgericht München, Registernr.: HRB 135854 Ust.Ident.Nr.: DE 215 296 022 <http://13457.cleverreach.de/c/9936054/b683f5ae3bd6>

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[FILM-Users 00316] FILM Club this Friday: Amelia Shoemark: "Electron tomography and immunofluorescence in the diagnosis of primary ciliary dyskinesia"
by Spitaler, Martin 18 Feb '13

18 Feb '13

Dear microscopists, also February is approaching its end, so this week is already FILM CLUB time again: Amelia Shoemark will talk about "Electron tomography and immunofluorescence in the diagnosis of primary ciliary dyskinesia". Friday 22 Feb 2013, 1pm, SAFB-120, Fleming Building, South Kensington. Don't miss out on another interesting presentation. See you all there, good imaging, Martin -- ________________________________ Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk<mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00315] Fwd: Fwd: "Microscopy in Focus" - SIGN UP NOW for our newest Science Webinar!
by Martin Spitaler 15 Feb '13

15 Feb '13

...forwarded for your information, Martin -------- Original Message -------- Subject: Fwd: "Microscopy in Focus" - SIGN UP NOW for our newest Science Webinar! Date: Thu, 14 Feb 2013 10:38:11 +0000 From: Magee, Tony <t.magee(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> For FILM mailing list. T Begin forwarded message: > *From:* Science Webinars <sciencewebinars(a)aaas-science.org > <mailto:sciencewebinars@aaas-science.org>> > *Date:* 14 February 2013 10:33:09 GMT > *To:* <T.MAGEE(a)IMPERIAL.AC.UK <mailto:T.MAGEE@IMPERIAL.AC.UK>> > *Subject:* *"Microscopy in Focus" - SIGN UP NOW for our newest Science > Webinar!* > *Reply-To:* Science Webinars <reply(a)mail.aaas-science.org > <mailto:reply@mail.aaas-science.org>> > > View on mobile or on web page > <http://app.aaas-science.org/e/es.aspx?s=1906&e=43587&elq=07375201320e4f22a7…> > > Science Webinar Series <http://webinar.sciencemag.org/> > > New complimentary webinar from /Science/: > > *Microscopy in Focus: The Art and Science of Image Quality* > > You are invited to hear our panel of experts on March 7, 2013, in this > live, online educational seminar. For more information and > complimentary registration visit: *webinar.sciencemag.org* > <http://webinars.sciencemag.org/webinar/archive/microscopy-focus> > > *Date: *Thursday, March 7, 2013 > *Time:* 9 a.m. UK, 10 a.m. CET, 5 p.m. China, 6 p.m. Japan, 4 a.m. > Eastern US > *Duration:* 1 hour > > Submit your questions in advance by sending them to webinar(a)aaas.org > <mailto:webinar@aaas.org>. > > <http://bit.ly/MicroscopyInFocus> > > *About This Webinar * > > High resolution microscopy has become a widespread and ubiquitous > technology in most life science laboratories and high quality images > have become an essential part of many publications. Technology such as > confocality can enhance image contrast by eliminating out of focus > light, but this is often at the expense of signal intensity and > increased phototoxicity. In this webinar our experts will discuss the > benefits and challenges of confocality, compare and contrast it with > other technologies such as deconvolution, and provide advice on which > technology is best for which application. > > During the webinar, our panelists will: > - Obtain an overview of confocal microscopy and its application in > basic research > - Learn about technologies such as deconvolution, which can help to > preserve maximum light input and provide better image quality > - Receive best practice advice for obtaining the best quality images, > regardless of the technique used > - Have their questions answered live by our respected thought-leaders! > > *Participants:* > > Jason Swedlow , Ph.D. > University of Dundee, > Dundee, U.K. > > John Murray, M.D., Ph.D. > Indiana University > Bloomington, IN > > Paul C. Goodwin, M.Sc > GE Healthcare > Issaquah, WA > > *Register at:* > *webinar.sciencemag.org* > <http://webinars.sciencemag.org/webinar/archive/microscopy-focus> > > Produced by the /Science//AAAS Custom Publishing Office and sponsored > by GE Healthcare. > > <http://bit.ly/MicroscopyInFocus> > > Manage Your E-mail Subscription Preferences > <http://aaas-science.org/ANTHONYMAGEE1?elqPURLPage=18&elq=07375201320e4f22a7…> > | Unsubscribe > <http://aaas-science.org/ANTHONYMAGEE1?elqPURLPage=18&elq=07375201320e4f22a7…> > Need help? Contact memuser(a)aaas.org <mailto:memuser@aaas.org> with > customer service code ELQ > > This e-mail was sent to: T.MAGEE(a)IMPERIAL.AC.UK > <mailto:T.MAGEE@IMPERIAL.AC.UK> > AAAS / /Science/ | 1200 New York Avenue NW | Washington, DC 20005 > | U.S.A. > +1 202-326-6417 | memuser(a)aaas.org <mailto:memuser@aaas.org> | > Privacy Policy > <http://www.sciencemag.org/site/help/about/privacy.xhtml?elq=07375201320e4f2…> >

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[FILM-Users 00314] COMMERCIAL Fwd: New ibidi products from Thistle Scientific
by Martin Spitaler 07 Feb '13

07 Feb '13

COMMERCIAL ------------------------------------------------------------------------ -------- Original Message -------- Subject: New ibidi products from Thistle Scientific Date: Tue, 5 Feb 2013 17:28:05 +0000 From: James Smith <James(a)thistlescientific.co.uk> Reply-To: <enquiries(a)thistlescientific.co.uk> To: Dr Martin Spitaler <m.spitaler(a)imperial.ac.uk> Celebrating 20 Years of Biometra Thermocyclers Dear Dr Spitaler, Here we are in 2013 and already ibidi are introducing new products to one of their most popular ranges. The 24-well m-plate with flat clear bottom was designed specifically with high throughput applications in mind. This product can also be used for a variety of routine cell based assays including fluorescence based work and live cell imaging. For product details, including ordering information and pricing, please click here <http://www.thistlescientific.co.uk/e-news/ibidi-jan-2013/IBIDI-Jan-2013-E-m…>. Should you require further information on this or any other ibidi product, please click here <http://ibidi.com/>. Kind regards, *Thistle Scientific Ltd* *DFDS House, Goldie Road* *Uddingston,* *Glasgow* *G71 6NZ* *Tel: 01698 338844 Fax: 01698 338880* *email: Enquiries <mailto:Enquiries@thistlescientific.co.uk>**(a)thistlescientific.co.uk* <mailto:Enquiries@thistlescientific.co.uk>

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