December 2013 - FILM-Users

[FILM-Users 00434] FILM application for Wellcome Trust Multi-User Equipment grant
by Martin Spitaler 31 Mar '14

31 Mar '14

Dear microscopists, following feedback from FILM users and Wellcome Trust grant holders (see summary at the bottom), the FILM steering committee has decided to use the open call for Wellcome Trust Multi-User Equipment grant applications to apply for upgrades / updates of existing equipment as well as some novel technology. The highest demand is currently for a new super-resolution microscope, which is luckily already funded by BBSRC, we are now in the process of testing the equipment to be purchased. Other demands stretched across a wide range of technologies and unfortunately also locations across the college, the committee tried to strike the best balance between all needs and decided to apply for the following package: * _New technology_: o an *automated high-throughput **m**icroscopy* system (e.g. Operetta or similar) o the high-throughput capacity, in combination with existing image processing / analysis capacity (e.g. Definiens) will satisfy the needs for both the high-content screening and to a large degree ImageStream needs * _Upgrade_: o *upgrade of the existing intravital microscope (FILM-CF3)* with options including high-sensitivity hybrid detectors, 405 laser, a cabinet for mouse handling, laser dispersion correction (detailed options to be checked with manufacturers) * _Support_: o *one full-time FILM staff post for Hammersmith* for the duration of the grant * _Replacement_: o *a high-sensitivity new-generation confocal* (similar to the Zeiss LSM-780 in Hammersmith), to be located in SAFB / South Kensington * _Possible options for small, complementary equipment add-ons_: o FleXcell Tensions System o ONIX microfluidic platform (if not already funded by other sources through other sources) o a high-precision multi-channel peristaltic pump system Obviously, we can't apply for everything on the list at the same time, some things will have to wait for the next equipment grant call. We now need to finalise the application - and for that *we need your support*: Those of you willing to support the application with your scientific interest and track record, please do get back to us soon - the deadline for the preliminary application is 2nd of December. And although the major bits of equipment to go on the application have been decided, if there are smaller add-ons missing on the list that would help with your microscopy in FILM, please let us know. Good imaging, Martin _*Information: *_ * http://www.wellcome.ac.uk/Funding/Biomedical-science/Funding-schemes/Strate… _*Summary of **m**icroscopy needs in Imperial College - FILM, feedback 2013*:_

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[FILM-Users 00450] ending the year with the Best Image Of The Year
by Martin Spitaler 20 Dec '13

20 Dec '13

Dear microscopists, I'm very happy to finish this busy and successful year with the *BEST FILM IMAGE OF THE YEAR*: *Priyanthi Dias' Fibroblasts* - congratulations, your new year will be full of music from your iPod nano. The full list of the to-twelve entries is in the attachment, and they will contribute to another stunning FILM calendar. All entrants will receive one for free, others are welcome to order one. For now the FILM crew wishes everybody a peaceful and refreshing Christmas time and start into the new year. We will be back for a new year full of exciting action (starting with the new super-resolution microscope and a new member of the FILM crew to help with it) in January (2nd Mark, 3rd Martin, 6th Steve). Best wishes, and much more good imaging next year, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00449] Fwd: Fluorescent Anti-Armenian Hamster
by Martin Spitaler 16 Dec '13

16 Dec '13

....can anybody help? Martin -------- Original Message -------- Subject: Fluorescent Anti-Armenian Hamster Date: Mon, 16 Dec 2013 18:27:54 +0000 From: Carlin, Leo M <l.carlin(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, Please could you send a request out to the FLIM list for me? Does anyone have a highly cross adsorbed (multiple species), or Fab fragment anti-armenian hamster secondary with Red or Fa- Red fluorophore that I could try a little of please? Best wishes, Leo -- Leo M. Carlin, Ph. D. E: mailto:l.carlin@imperial.ac.uk T: +44 (0) 20 7594 3323 W: www.imperial.ac.uk/people/l.carlin <http://www.imperial.ac.uk/people/l.carlin> Lecturer in Respiratory Science Leukocyte Biology Section, NHLI Imperial College London Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ, UK

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[FILM-Users 00448] red fluorescent streptavidin
by Gregory, Lisa 11 Dec '13

11 Dec '13

Dear All, Does anyone have a red fluoresent streptavidin that I could take a couple of ul of to test for a dual staining protocol please? Many thanks, Lisa Dr Lisa Gregory Leukocyte Biology Section National Heart & Lung Institute Faculty of Medicine Imperial College London South Kensington Campus Sir Alexander Fleming Building London SW7 2AZ Tel: +44 (0)20 7594 3129 Fax: +44 (0)20 7594 3119 Email: l.gregory(a)imperial.ac.uk ________________________________ From: film-users-bounces(a)imperial.ac.uk [film-users-bounces(a)imperial.ac.uk] on behalf of Martin Spitaler [m.spitaler(a)imperial.ac.uk] Sent: 11 December 2013 09:27 To: film-users Subject: [FILM-Users 00447] TODAY: Eric Betzig lecture super-resolution and light sheet microscopy on 11th December 2013 -------- Original Message -------- Subject: Eric Betzig lecture super-resolution and light sheet micrscopy on 11th December 2013 Date: Tue, 5 Nov 2013 15:35:16 +0000 From: French, Paul (PHOT) M W (Photonics) <paul.french(a)imperial.ac.uk><mailto:paul.french@imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk><mailto:m.spitaler@imperial.ac.uk> Dear Martin, Eric Betzig is one of the leading pioneers of both PALM microscopy for super-resolved imaging and light sheet microscopy for rapid imaging of live organisms. This promises to be an excellent talk. Please could you advertise it, e.g. through your FILM mailing list and the London super-resolution club. Best wishes, Paul Leica Scientific Forum UK presents Eric Betzig Wednesday, December 11, 2013 at 17:00h - Chair: Paul French Venue: Imperial College London, Lecture Theatre 3, Blackett Laboratory, Prince Consort Road, London SW7 2BB Eric Betzig<http://www.janelia.org/lab/betzig-lab> Janelia Farm Research Campus, Howard Hughes Medical Insitute, Ashburn, VA "Imaging Life at High Spatiotemporal Resolution" Optical microscopy has remained at the forefront of biological discovery for centuries. However, as our understanding of biological systems as increased, so has the complexity of our questions and the need for more advanced optical tools to answer them. For example, there is a hundred-fold gap between the resolution of conventional optical microscopy and the scale at which molecules self-assemble to form sub-cellular structures. Furthermore, as we attempt to peer more closely at the dynamic complexity of living systems, the actinic glare of our microscopes can adversely influence or even kill the specimens we hope to study. Finally, the heterogeneity of life, ranging from organelles within single cells to specialized cell types within tissues and organs, can seriously impede our ability to image at high resolution, due to the resulting warping and scattering of light rays. I will describe three areas focused on addressing these challenges: super-resolution microscopy for imaging specific proteins within cells down to near-molecular resolution; plane illumination microscopy using non-diffracting beams for noninvasive imaging of three-dimensional dynamics within live cells and embryos; and adaptive optics to recover optimal images from within optically heterogeneous specimens Invitation poster: <https://workspace.imperial.ac.uk/imagingfacility/Public/seminar.pdf>

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[FILM-Users 00426] Fwd: Eric Betzig lecture super-resolution and light sheet microscopy on 11th December 2013
by Martin Spitaler 11 Dec '13

11 Dec '13

...forwarded for your information... Martin -------- Original Message -------- Subject: Eric Betzig lecture super-resolution and light sheet micrscopy on 11th December 2013 Date: Tue, 5 Nov 2013 15:35:16 +0000 From: French, Paul (PHOT) M W (Photonics) <paul.french(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, Eric Betzig is one of the leading pioneers of both PALM microscopy for super-resolved imaging and light sheet microscopy for rapid imaging of live organisms. This promises to be an excellent talk. Please could you advertise it, e.g. through your FILM mailing list and the London super-resolution club. Best wishes, Paul Leica Scientific Forum UK presents Eric Betzig *Wednesday, December 11, 2013 at 17:00h - Chair: Paul French* Venue: Imperial College London, Lecture Theatre 3, Blackett Laboratory, Prince Consort Road, London SW7 2BB Eric Betzig <http://www.janelia.org/lab/betzig-lab> Janelia Farm Research Campus, Howard Hughes Medical Insitute, Ashburn, VA "Imaging Life at High Spatiotemporal Resolution" Optical microscopy has remained at the forefront of biological discovery for centuries. However, as our understanding of biological systems as increased, so has the complexity of our questions and the need for more advanced optical tools to answer them. For example, there is a hundred-fold gap between the resolution of conventional optical microscopy and the scale at which molecules self-assemble to form sub-cellular structures. Furthermore, as we attempt to peer more closely at the dynamic complexity of living systems, the actinic glare of our microscopes can adversely influence or even kill the specimens we hope to study. Finally, the heterogeneity of life, ranging from organelles within single cells to specialized cell types within tissues and organs, can seriously impede our ability to image at high resolution, due to the resulting warping and scattering of light rays. I will describe three areas focused on addressing these challenges: super-resolution microscopy for imaging specific proteins within cells down to near-molecular resolution; plane illumination microscopy using non-diffracting beams for noninvasive imaging of three-dimensional dynamics within live cells and embryos; and adaptive optics to recover optimal images from within optically heterogeneous specimens Invitation poster: <https://workspace.imperial.ac.uk/imagingfacility/Public/seminar.pdf>

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[FILM-Users 00446] open position for super-resolution post (RB158-13)
by Martin Spitaler 06 Dec '13

06 Dec '13

Dear microscopists, as part of the funding for the new super-resolution microscope we also have funding for a support staff for a year to help with all aspects from installation to testing, maintenance, quality checks, user training, data handling, data processing, data workflow and integration. An essential part of the job will be to collaborate with the Bioinformatics Support Service and authors of analysis software (open-source and commercial) to integrate the super-resolution data workflow, e.g. via batch processing tools, Omero scripts etc. Although fixed-term, this is a rather special opportunity to get involved in an exciting super-resolution project, and being part of our lively facility, the person can also acquire invaluable experience in all aspects of facility life and light microscopy in biomedical research. For further details see online job description (RB158-13) <https://www4.ad.ic.ac.uk/OA_HTML/OA.jsp?page=/oracle/apps/irc/candidateSelf…..>, or contact Steve or myself (contact info on FILM website > CONTACTS <http://www3.imperial.ac.uk/imagingfacility/location_contacts>). Good imaging, Martin ------------------------------------------------------------------------ JOB DESCRIPTION *Post Title: *Super-Resolution Microscopy Technician** *Institute:*National Heart and Lung Institute** *Section: *Facility for Imaging by Light Microscopy [FILM]** *Job Family and Level:*Technical Services, Level 3a** *Salary Range:*£28,770 - £32,750 per annum** *Responsible To:*Stephen Rothery (FILM Super-Resolution Specialist)** *Works closely with:*Martin Spitaler (FILM Facility Manager) Mark Scott (FILM staff)** *Working Relationships: *Martin Spitaler (FILM Facility Manager) Mark Scott (FILM staff) Professor Tony Magee (FILM Academic Lead), BSS users** *Location:*South Kensington** *Funding:*Biotechnology and Biological Services Research Council** *Duration:*Fixed-term for**12 months** __ _Summary of Post_ This post involves setup, maintenance and user support for a state-of-the art super-resolution microscope and requires a high standard of technical expertise in the area of super-resolution microscopy. You will have responsibility for the day-to-day co-ordination, running and maintenance of the super-resolution microscope. A second element of the post will be support in data handling and processing, which will comprise optimising and implementing algorithms, and liaison with the Bioinformatics Support Service (BSS) to develop an integrated data workflow including data acquisition software, image database, central processing via established software tools (commercial and open source) etc. This will require automation of workflows through scripting. Expertise in computer programming will be essential. _Duties and Responsibilities_ ·Provide up-to-date technical expertise for the setup, optimisation and routine use of a new state-of-the-art super-resolution microscope ·Perform routine checks, maintenance and protocol optimisation for super-resolution microscopy ·Liaise with the manufacturers of the microscope to solve any technical problems ·Give users advice, training and support to a very high standard in all aspects of super-resolution microscopy, from sample preparation to labelling strategies, image acquisition, data processing, interpretation, visualisation and quantification ·Help with general duties in the FILM microscopy facilities, also related to other microscopes ·Coordinate and oversee booking and training of facility users ·Ensure that all work done in the laboratory complies with the applicable regulations concerning biological and laser safety ·Liaise with BSS to set up a fully integrated data workflow, including data safety and efficient processing ·Use existing software and develop purpose-built scripts to automate large-scale super-resolution data processing and analysis ·Liaise with manufacturers of commercial tools and authors of established open-source algorithms to integrate them in the data handling workflow ·Contribute to presentations and public education events related to super-resolution microscopy ·Effective liaison with other members of the FILM team ·Any other duties which may arise in the facility commensurate with the grade of the post *To observe and comply with all College policies and regulations, including the key policies and procedures on Confidentiality, Conflict of Interest, Data Protection, Equal Opportunities, Financial Regulations, Health and Safety, Imperial Expectations (for new leaders, managers and supervisors), Information Technology, Private Engagements and Register of Interests, and Smoking.* ** *To undertake specific safety responsibilities relevant to individual roles, as set out on the College Website Health and Safety Structure and Responsibilities page (http://www3.imperial.ac.uk/safety/policies/organisationandarrangements).* ** *Job descriptions cannot be exhaustive and the post-holder may be required to undertake other duties, which are broadly in line with the above key responsibilities.* ** *Imperial College is committed to equality of opportunity and to eliminating discrimination. All employees are expected to adhere to the principles set out in its Equal Opportunities in Employment Policy, Promoting Race Equality Policy and all other relevant guidance/practice frameworks.* * * IMPERIAL COLLEGE LONDON *PERSON SPECIFICATION* *_Qualifications_* Essential ·Bachelor Degree in a technical or biological area related to super-resolution microscopy, or equivalent vocational qualification or equivalent experience in knowledge and experience *_Knowledge and Experience_* Essential ·Proven experience in localisation (PALM / STORM) and / or structured illumination (SIM) super-resolution microscopy ·Experience with image data handling and processing, e.g. FIJI / ImageJ, QuickPALM, RapidSTORM, RainSTORM, OMERO ·Experience in computer programming or scripting, e.g. Java, Python, Matlab Desirable ·Experience in supporting and training microscope users ·Experience in setup and optimisation of super-resolution microscopes and application protocols *_Skills and Abilities_* Essential ·Excellent super-resolution microscopy skills ·Excellent computer skills ·Excellent organisational skills ·Excellent attention to detail ·Excellent interpersonal and supervisory skills, and an ambition to run the facility service for the best benefit of microscope users ·Excellent written and oral communication skills ·Proven skills at problem-solving and overcoming obstacles ·Ability to use own initiative and work independently and as part of a team, as required ·Proven ability to prioritise, plan ahead and manage a high volume of work to meet deadlines, both prescribed and self-imposed, and ensure that the facility runs in an efficient and timely manner ·Ability and willingness to adapt to changing timetables or work patterns as dictated by changing circumstances ·Ability to work under pressure while maintaining a high degree of accuracy *_Other_* Essential ·An open, flexible and positive approach to working in a constantly changing environment ·Prepared to work unsociable hours as work demands from time to time -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00445] Fwd: [lsr-group] Aki Kusumi
by Martin Spitaler 06 Dec '13

06 Dec '13

...forwarded for your information, Martin ------------------------------------------------------------------------ -------- Original Message -------- Subject: [lsr-group] Aki Kusumi Date: Thu, 5 Dec 2013 10:44:48 +0000 From: Owen, Dylan <dylan.owen(a)kcl.ac.uk> To: lsr-group(a)imperial.ac.uk <lsr-group(a)imperial.ac.uk> Aki Kusumi is giving a talk at King's next week which some of you may be interested in... D Department of Chemistry Seminar Professor Aki Kusumi Institute for Integrated Cell-Material Sciences (iCeMS), Institute for Frontier Medical Sciences Kyoto University "Hierarchical and dynamic organization of the plasma membrane for signal transduction revealed by single-molecule tracking" 10th Dec 2013, 16:00, NHH G8, Kings College Host: Roger Morris <https://workspace.imperial.ac.uk/imagingfacility/Public/seminar-131210.pdf> <http://www.nature.com/nature/journal/v433/n7027/full/433680a.html>

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[FILM-Users 00440] FILM Club next week Wed 4 Dec 4.30pm: Vania Braga lab - "Challenges and potential solutions to quantification of cell-cell contacts"
by Martin Spitaler 04 Dec '13

04 Dec '13

Dear microscopists, some of you might have wondered what happened to this month's FILM Club (though most probably won't have noticed, in this busy time of the year). Unfortunately, due to unforeseen circumstances it had to be postponed by a week - so it will happen NEXT WEEK: * Vania Braga's lab *will talk about the experience they acquired over the last few years with *"Challenges and potential solutions to quantification of cell-cell contacts"*. This large project involved a large range of microscopy techniques including high-throughput screening, handling of large datasets and making sense of the data in an automated way. Wednesday 4 December 2013, 4.30pm, SAFB-121 (South Kensington) >>> Free drinks and nibbles will be served!! See you there, good imaging, Martin /PS: This Friday (29 Nov), all FILM staff will be away testing various new microscopes (for which luckily funding has been granted), so please don't plan any experiments requiring our assistance./ -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00443] Fwd: Marie Curie Innovative Doctoral Program Vacancies
by Martin Spitaler 03 Dec '13

03 Dec '13

...forwarded for your information... Martin -------- Original Message -------- Subject: Marie Curie Innovative Doctoral Program Vacancies Date: Mon, 2 Dec 2013 20:08:10 +0000 From: Jason Swedlow <j.r.swedlow(a)DUNDEE.AC.UK> Reply-To: Jason Swedlow <j.r.swedlow(a)DUNDEE.AC.UK> To: <UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK> Dear All- Dundee University are currently recruiting 13 Marie Curie PhD posts as part of the Innovative Doctoral Program PHOQUS that aims to develop new tools for quantitative imaging. The program includes strong interdisciplinary themes between cell and developmental biology, biophysics, and photonics. We would like to raise awareness of these opportunities to the wider imaging community so that we attract the best possible candidates. Successful candidates will receive a generous PhD fellowship in the region of £38,000 per annum, participate in an extensive interdisciplinary training programme and undertake secondments to Industrial and/or Academic Partners. More details are at http://www.phoqus.eu Questions & queries: PHOQUS(a)dundee.ac.uk <mailto:PHOQUS@dundee.ac.uk> Application deadline: 6^th Jan 2014 Cheers, Jason -------------------- Centre for Gene Regulation & Expression | Open Microscopy Environment | University of Dundee Phone: +44 (0) 1382 385819 email: j.swedlow(a)dundee.ac.uk <mailto:j.swedlow@dundee.ac.uk> Web: http://www.lifesci.dundee.ac.uk/gre/staff/jason-swedlow Open Microscopy Environment: http://openmicroscopy.org <http://openmicroscopy.org/> The University of Dundee is a registered Scottish Charity, No: SC015096 -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT --

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[FILM-Users 00441] Fwd: On Demand webcast: Real time imaging of cells with Super Resolution Microscopy.
by Martin Spitaler 02 Dec '13

02 Dec '13

...forwarded for your information, Martin Begin forwarded message: > *From:* Nature Publishing Group > <Nature.Publishing.Group(a)ealert.nature.com > <mailto:Nature.Publishing.Group@ealert.nature.com>> > *Date:* 29 November 2013 10:03:37 GMT > *To:* <t.magee(a)imperial.ac.uk <mailto:t.magee@imperial.ac.uk>> > *Subject:* *On Demand webcast: Real time imaging of cells with Super > Resolution Microscopy.* > *Reply-To:* <Nature.Publishing.Group(a)ealert.nature.com > <mailto:Nature.Publishing.Group@ealert.nature.com>> > > Macmillan Scientific Communication Webcast > If you are unable to see the message below, click here to view > <http://links.ealert.nature.com/servlet/MailView?ms=NDQ0NjkyOTgS1&r=MTc3MDMy…>. > > > nature.com webcasts > <http://links.ealert.nature.com/ctt?kn=10&ms=NDQ0NjkyOTgS1&r=MTc3MDMyNTQyMwS…> > > > Custom webcast: sponsor retains sole responsibility for content > > *The latest innovations > **in real-time imaging of > cells with Super > Resolution Microscopy ** > > A discussion into new technical innovations for > Super Resolution Microscopy** > > > Now available On Demand > * > > > > ** > Speakers: > *Dr Jennifer Lippincott-Schwartz* > NIH Distinguished Investigator. > > *Dr Orla Hanrahan* > Andor Technology. > > *Dr Susan Cox* > King's College, London. > > *Register for FREE > <http://links.ealert.nature.com/ctt?kn=20&ms=NDQ0NjkyOTgS1&r=MTc3MDMyNTQyMwS…>* > > Due to the diffraction limit, standard light microscopes can at best > resolve features which are around 200nm apart, meaning that they are > unsuited to many biological applications. In this webcast, three > expert speakers will discuss super resolution microscopy, a rapidly > developing field that is breaking the diffraction limit to enable the > close examination of finely detailed biological samples. > > The current focus of the Super Resolution Microscopy community is to > increase the utility of existing localisation microscopy techniques > such as photo-activated localization microscopy (PALM) and stochastic > optical reconstruction microscopy (STORM) – in these methods, > fluorophores within a sample emit low levels of light, which is imaged > many thousands of times. This creates a single composite image with > resolution at the true molecular length scale. However, this process > is slow, meaning that these techniques are not best suited to > real-time imaging of cells and tissues. > > Our first speaker, Dr Jennifer Lippincott-Schwartz, will discuss > research currently underway using genetically-encoded photoactivatable > proteins (PA-FPs) for superresolution imaging which has dramatically > expanded the study of cellular organization, function and dynamics. > Our second speaker, Dr Susan Cox will go into some detail on the > algorithms which facilitate high resolution imaging of live cell > dynamics at the nanoscale and lastly Dr Orla Hanrahan will discuss > fast and sensitive detector technologies which are suitable for the > low light intensities of the single molecule techniques and the speed > required for live cell super resolution applications. > > *Register for FREE > <http://links.ealert.nature.com/ctt?kn=20&ms=NDQ0NjkyOTgS1&r=MTc3MDMyNTQyMwS…> > > Browse through our webcasts > <http://links.ealert.nature.com/ctt?kn=10&ms=NDQ0NjkyOTgS1&r=MTc3MDMyNTQyMwS…> > > #MSCwebcasts * > > Sponsor Banner > <http://links.ealert.nature.com/ctt?kn=11&ms=NDQ0NjkyOTgS1&r=MTc3MDMyNTQyMwS…> > > Sponsor Banner Macmillan > <http://links.ealert.nature.com/ctt?kn=4&ms=NDQ0NjkyOTgS1&r=MTc3MDMyNTQyMwS2…> > > > Share: > <http://links.ealert.nature.com/s2s?m=44469298&r=MTc3MDMyNTQyMwS2&rj=MjE1Nzg…>Facebook > <http://links.ealert.nature.com/s2s?m=44469298&r=MTc3MDMyNTQyMwS2&rj=MjE1Nzg…><http://links.ealert.nature.com/s2s?m=44469298&r=MTc3MDMyNTQyMwS2&rj=MjE1Nzg…>Twitter > <http://links.ealert.nature.com/s2s?m=44469298&r=MTc3MDMyNTQyMwS2&rj=MjE1Nzg…><http://links.ealert.nature.com/s2s?m=44469298&r=MTc3MDMyNTQyMwS2&rj=MjE1Nzg…>LinkedIn > <http://links.ealert.nature.com/s2s?m=44469298&r=MTc3MDMyNTQyMwS2&rj=MjE1Nzg…><http://links.ealert.nature.com/s2s?m=44469298&r=MTc3MDMyNTQyMwS2&rj=MjE1Nzg…>Digg > <http://links.ealert.nature.com/s2s?m=44469298&r=MTc3MDMyNTQyMwS2&rj=MjE1Nzg…><http://links.ealert.nature.com/s2s?m=44469298&r=MTc3MDMyNTQyMwS2&rj=MjE1Nzg…>StumbleUpon > <http://links.ealert.nature.com/s2s?m=44469298&r=MTc3MDMyNTQyMwS2&rj=MjE1Nzg…> > > As a registered user of Nature Publishing Group's Web sites, our > database indicates that you have opted-in to receive third-party > emails. 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