November 2013 - FILM-Users

[FILM-Users 00434] FILM application for Wellcome Trust Multi-User Equipment grant
by Martin Spitaler 31 Mar '14

31 Mar '14

Dear microscopists, following feedback from FILM users and Wellcome Trust grant holders (see summary at the bottom), the FILM steering committee has decided to use the open call for Wellcome Trust Multi-User Equipment grant applications to apply for upgrades / updates of existing equipment as well as some novel technology. The highest demand is currently for a new super-resolution microscope, which is luckily already funded by BBSRC, we are now in the process of testing the equipment to be purchased. Other demands stretched across a wide range of technologies and unfortunately also locations across the college, the committee tried to strike the best balance between all needs and decided to apply for the following package: * _New technology_: o an *automated high-throughput **m**icroscopy* system (e.g. Operetta or similar) o the high-throughput capacity, in combination with existing image processing / analysis capacity (e.g. Definiens) will satisfy the needs for both the high-content screening and to a large degree ImageStream needs * _Upgrade_: o *upgrade of the existing intravital microscope (FILM-CF3)* with options including high-sensitivity hybrid detectors, 405 laser, a cabinet for mouse handling, laser dispersion correction (detailed options to be checked with manufacturers) * _Support_: o *one full-time FILM staff post for Hammersmith* for the duration of the grant * _Replacement_: o *a high-sensitivity new-generation confocal* (similar to the Zeiss LSM-780 in Hammersmith), to be located in SAFB / South Kensington * _Possible options for small, complementary equipment add-ons_: o FleXcell Tensions System o ONIX microfluidic platform (if not already funded by other sources through other sources) o a high-precision multi-channel peristaltic pump system Obviously, we can't apply for everything on the list at the same time, some things will have to wait for the next equipment grant call. We now need to finalise the application - and for that *we need your support*: Those of you willing to support the application with your scientific interest and track record, please do get back to us soon - the deadline for the preliminary application is 2nd of December. And although the major bits of equipment to go on the application have been decided, if there are smaller add-ons missing on the list that would help with your microscopy in FILM, please let us know. Good imaging, Martin _*Information: *_ * http://www.wellcome.ac.uk/Funding/Biomedical-science/Funding-schemes/Strate… _*Summary of **m**icroscopy needs in Imperial College - FILM, feedback 2013*:_

1 1

[FILM-Users 00426] Fwd: Eric Betzig lecture super-resolution and light sheet microscopy on 11th December 2013
by Martin Spitaler 11 Dec '13

11 Dec '13

...forwarded for your information... Martin -------- Original Message -------- Subject: Eric Betzig lecture super-resolution and light sheet micrscopy on 11th December 2013 Date: Tue, 5 Nov 2013 15:35:16 +0000 From: French, Paul (PHOT) M W (Photonics) <paul.french(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, Eric Betzig is one of the leading pioneers of both PALM microscopy for super-resolved imaging and light sheet microscopy for rapid imaging of live organisms. This promises to be an excellent talk. Please could you advertise it, e.g. through your FILM mailing list and the London super-resolution club. Best wishes, Paul Leica Scientific Forum UK presents Eric Betzig *Wednesday, December 11, 2013 at 17:00h - Chair: Paul French* Venue: Imperial College London, Lecture Theatre 3, Blackett Laboratory, Prince Consort Road, London SW7 2BB Eric Betzig <http://www.janelia.org/lab/betzig-lab> Janelia Farm Research Campus, Howard Hughes Medical Insitute, Ashburn, VA "Imaging Life at High Spatiotemporal Resolution" Optical microscopy has remained at the forefront of biological discovery for centuries. However, as our understanding of biological systems as increased, so has the complexity of our questions and the need for more advanced optical tools to answer them. For example, there is a hundred-fold gap between the resolution of conventional optical microscopy and the scale at which molecules self-assemble to form sub-cellular structures. Furthermore, as we attempt to peer more closely at the dynamic complexity of living systems, the actinic glare of our microscopes can adversely influence or even kill the specimens we hope to study. Finally, the heterogeneity of life, ranging from organelles within single cells to specialized cell types within tissues and organs, can seriously impede our ability to image at high resolution, due to the resulting warping and scattering of light rays. I will describe three areas focused on addressing these challenges: super-resolution microscopy for imaging specific proteins within cells down to near-molecular resolution; plane illumination microscopy using non-diffracting beams for noninvasive imaging of three-dimensional dynamics within live cells and embryos; and adaptive optics to recover optimal images from within optically heterogeneous specimens Invitation poster: <https://workspace.imperial.ac.uk/imagingfacility/Public/seminar.pdf>

1 1

[FILM-Users 00440] FILM Club next week Wed 4 Dec 4.30pm: Vania Braga lab - "Challenges and potential solutions to quantification of cell-cell contacts"
by Martin Spitaler 04 Dec '13

04 Dec '13

Dear microscopists, some of you might have wondered what happened to this month's FILM Club (though most probably won't have noticed, in this busy time of the year). Unfortunately, due to unforeseen circumstances it had to be postponed by a week - so it will happen NEXT WEEK: * Vania Braga's lab *will talk about the experience they acquired over the last few years with *"Challenges and potential solutions to quantification of cell-cell contacts"*. This large project involved a large range of microscopy techniques including high-throughput screening, handling of large datasets and making sense of the data in an automated way. Wednesday 4 December 2013, 4.30pm, SAFB-121 (South Kensington) >>> Free drinks and nibbles will be served!! See you there, good imaging, Martin /PS: This Friday (29 Nov), all FILM staff will be away testing various new microscopes (for which luckily funding has been granted), so please don't plan any experiments requiring our assistance./ -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 2

[FILM-Users 00439] Fwd: Jacco Van Rheenen seminar Monday Dec 2nd, 11am, SAF119
by Martin Spitaler 26 Nov '13

26 Nov '13

...forwarded for your information, Martin -------- Original Message -------- Subject: Jacco Van Rheenen seminar Monday Dec 2nd, 11am, SAF119 Date: Tue, 26 Nov 2013 20:29:06 +0000 From: Lo Celso, Cristina <c.lo-celso(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear all, *Dr Jacco van Rheenen*, from the Ubrecht Institute (Utrecht, Netherlands) will be giving a seminar on *Monday December 2nd at 11am* in *room 119 SAFB (South Kensington)*. The title of the seminar is: _*Intravital imaging of cancer plasticity through imaging windows*._ Please join for the seminar if relevant to you, let me know (c.lo-celso(a)imperial.ac.uk) if you would like some time to talk to Jacco during the day, and please pass this message to your groups and to anybody you think may be interested. Best wishes, Cristina Cristina Lo Celso, PhD Division of Cell and Molecular Biology Imperial College London South Kensington Campus Sir Alexander Fleming Building London SW7 2AZ United Kingdom Phone: +44 (0) 207 594 5359 Fax: +44 (0) 207 584 2056

1 0

[FILM-Users 00438] Fwd: OMERO server upgrade
by Martin Spitaler 26 Nov '13

26 Nov '13

...forwarded for your information, Martin -------- Original Message -------- Subject: OMERO server upgrade Date: Tue, 26 Nov 2013 11:36:45 +0000 From: Mark Woodbridge <m.woodbridge(a)imperial.ac.uk> To: bsshelp <bsshelp(a)imperial.ac.uk> There will be a brief interruption to the BSS OMERO service at 9.30am on Friday 29th November whilst it is upgraded to version 4.4.9. Please contact me if you have any questions. More information about OMERO is available on the BSS web pages: http://www3.imperial.ac.uk/bioinfsupport/resources/data_management/omero Regards, Mark.

1 0

[FILM-Users 00437] files deleted on temporary server
by Martin Spitaler 26 Nov '13

26 Nov '13

Dear microscopists, this is a reminder that the FILM server <\\film-nas.med.ic.ac.uk\SCRATCH> is only to be used as a temporary storage space to move files around, NOT to store any data. Files on the server can and will be removed whenever space is needed WITHOUT NOTICE. This is to make sure all users can smoothly use the microscopes without worrying about copying data around. If you need space to STORE your data, we highly recommend to use the double-backed up space provided by the Bioinformatics Support Service, including the very useful OMERO Image Database. That's what we use for our image data in FILM, because it's the best solution we have found. For more information see FILM website / GUIDELINES / DATA / DATA MANAGEMENT for more information. <http://www3.imperial.ac.uk/imagingfacility/facility_guidelines/data_managem…> Right now, the server is again nearing capacity, so we will delete files to make space for new data over the next few days. If there is anything you need, please move it elsewhere quickly. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 0

[FILM-Users 00436] eYFP construct needed
by Martin Spitaler 22 Nov '13

22 Nov '13

Forwarded on behalf of Morgan Beeby - can anybody help? Best regards, Martin -------- Original Message -------- Subject: FW: fluorescent protein pointers? Date: Wed, 20 Nov 2013 12:44:07 +0000 From: Beeby, Morgan <m.beeby(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, Apologies for the harassment, but I wondered if you had any thoughts on possible people to talk to regarding obtaining eYFP (or other fluorescent protein)-encoding vectors? thanks! Morgan

1 0

[FILM-Users 00425] FILM Image Of The Year 2013-14
by Martin Spitaler 22 Nov '13

22 Nov '13

Dear microscopists, we are slowly nearing the end of the year again, so it's time for our annual competition again, the FILM IMAGE OF THE YEAR 2013-14! Again, thanks to generous sponsorship from Molecular Probes / Life Technologies, attractive prizes are waiting: *Winner *- an*iPod Nano*! ...for those long hours at the microscope _Runners-up_: a beautiful _A3 poster calendar_, worth £23! *Deadline* for submission of your images: *Friday 29th of December 2012* _*Judges *_ As in previous years, we have a prestigious panel of independent judges to select your best images: Kathy Nobel, Tate Modern Alex Ball, Natural History Museum Tatiana Schofield, Imperial College Consultants Melissa Belli, artist _ _ _Rules for participation_ * Up to 3 images per participant; they must show different samples (not different versions of the same image) * Images must have been taken on FILM microscopes (original data files must be made available) * Images will be judged purely on their visual impression, they do not need to show any scientific results, but a description of the scientific context is encouraged (and might make it more interesting for the judges) * The best possible visualisation of the image data is encouraged (e.g. contrast enhancement, look-up tables), but manipulation of pixel data is not allowed - the scientific content must still visible. Examples of manipulations not allowed are adding / removing of objects (retouching) or collages (copying objects together) * By submitting the images, participants grant FILM the right to make the entries publicly available on the FILM website, use them for the calendars or other presentations describing research peformed in the facility; please make sure they don't contain any identifiable unpublished information For inspirations from previous years and details see the website: <http://www3.imperial.ac.uk/imagingfacility/research/gallery/film_image_of_t…> So don't miss out, get your best microscopy images and go for the prizes! Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 1

[FILM-Users 00433] Fwd: Molecular Medicine & Cell Biology Seminar Series | Tue 12 Nov 2013 | 12.00 - 1.00pm room 120 SAF
by Martin Spitaler 11 Nov '13

11 Nov '13

...forwarded for your information Martin Flyer: <https://workspace.imperial.ac.uk/imagingfacility/Public/seminar-131112b.pdf> -------- Original Message -------- Subject: Molecular Medicine & Cell Biology Seminar Series | Tue 12 Nov 2013 | 12.00 - 1.00pm room 120 SAF Date: Mon, 11 Nov 2013 10:55:08 +0000 From: Enright, Catherine E <c.enright(a)imperial.ac.uk> Molecular Medicine & Cell Biology Seminar Series Molecular Medicine | National Heart and Lung Institute & Cell Biology | Division of Medicine Faculty of Medicine | Imperial College London *Dr Elisabeth Ehler** *The Randall Centre of Cell and Molecular Biophysics, King's College London. *'Formin-g and maintaining myofibrils in the heart'** * Host Dr Pradeep Luther *Tuesday 12 November 2013 | 12:00-1:00pm** **Room 120 | Sir Alexander Fleming Building | South Kensington Campus** *ALL WELCOME

1 0

[FILM-Users 00423] Phase Focus demo
by Martin Spitaler 11 Nov '13

11 Nov '13

Dear microscopists, see below the invitation to a demonstration of a new microscope technology, Ptychography, by Joanne Whetstone from the company Phasefocus. It's label free and three-dimensional, so potentially interesting to a range of users. Good imaging, Martin -------- Original Message -------- Subject: Re: Phase Focus demo Date: Mon, 28 Oct 2013 14:44:54 +0000 From: Joanne Whetstone <joanne.whetstone(a)phasefocus.com> *==> PDF version of this invitation*: <https://workspace.imperial.ac.uk/imagingfacility/Public/seminar.pdf> Phasefocus would like to invite you to a presentation on Monday 11th November at 12pm, and a demonstration of their high contrast label-free live cell imaging system on Tuesday 12th or Wednesday 13th November 2013. The Phasefocus Virtual Lens® uses a technique known as Ptychography, allowing phase imaging of cells in high contrast, without using labels, and free of artefacts. This method adds a further quantitative dimension to phase imaging, and is able to provide numerical information on cell cycle analysis, cell thickness, and cell volume, which has traditionally been measured using fluorescence. The system also uses low laser power allowing long term timelapse experiments to be performed. PRESENTATION Monday 11th November 12:00 Room SAFB-121, South Kensington Campus, Exhibition Road, London SW7 2AZ Refreshments will be provided DEMONSTRATION Room SAFB-530, South Kensington Campus, Exhibition Road, London SW7 2AZ We want you all to have the opportunity to see the system and ask questions, the easiest way to do this is to allocate timeslots for you to sign up for. These are: Tues12th and Wed 13th November: 09:30, 11:00, 13:00, 14:30, 16:00 To make a booking, please contact joanne.whetstone(a)phasefocus.com, 07748 963287. We are flexible, so if these times don't suit you, please contact us and we will do our best to accommodate you. Joanne Whetstone Sales & Marketing Manager -- Phase Focus Logo <http://www.phasefocus.com/> *Joanne Whetstone *Sales & Marketing Manager PHONE: +44 (0)114 286 6364 MOBILE:_+44 (0) 7748 963287_ WEB: www.phasefocus.com <http://www.phasefocus.com/> *Phasefocus Limited* Electric Works Sheffield Digital Campus Sheffield, UK S1 2BJ Facebook <https://www.facebook.com/pages/Phase-Focus/434726259956299?fref=ts>Twitter <https://twitter.com/PhaseFocus1>LinkedIn <http://www.linkedin.com/company/1290490>Phasefocus Blog <http://phasefocus.blogspot.co.uk/>Phasefocus Youtube Channel <http://www.youtube.com/channel/UCuiyDWj5fmp6VfXiflGSGyA> This email is confidential and may also be privileged. Unless you are the intended recipient, you may not copy, forward or otherwise use it, or disclose it or its contents to anyone else. If you have received this email in error please notify us immediately and delete it from your system. ------------------------------------------------------------------------ Company registered in England No. 05856137. Reg'd Office: 40 Leavy Greave Road, Sheffield, South Yorkshire, S3 7RD

1 1