September 2012 - FILM-Users

[FILM-Users 00257] new FILM CLUB season starts this Friday 1pm
by Martin Spitaler 28 Sep '12

28 Sep '12

Dear microscopists, it's end of September already, so time to re-start the new FILM CLUB season. As already traditional, we are kicking off with a report about recent microscopy meetings, ELMI, EMC and others. I will report about developments relevant for FILM users, new labels, sample preparation tricks, new technology coming up. All things that could make your life easier, understand new technologies better and stay up to date. The FILM CLUB will be on Friday 28 September 2012, 13.00 in room SAFB-120 (South Kensington campus). For those new to FILM: The FILM CLUB is a monthly meeting of FILM users (but open to anyone) with the aim of getting to know each other and all the wonderful things being done on the FILM microscopes, stimulate exchange of expertise, enable new collaborations and generally improve the knowledge and understanding of microscopy techniques. We've had many very interesting presentations in the past, as you can see on our website (http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/news#film…) and many more will follow this year. IMPORTANT: We need YOU. All of you who have done all these exciting experiments in the past, please come forward and present them to other FILM users, so you valuable experience is not lost! See you on Friday, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00259] Fwd: Seminars Lecturer in Parasitology/Vector Biology - 27-28/09/2012
by Martin Spitaler 26 Sep '12

26 Sep '12

...forwarded for your information; several of the people do very imaging-based research which could be interesting to FILM users, Martin -------- Original Message -------- Subject: Seminars Lecturer in Parasitology/Vector Biology - 27-28/09/2012 Date: Wed, 26 Sep 2012 14:37:20 +0100 From: Nurboja, Sandrine A C <s.nurboja(a)imperial.ac.uk> To: DoLS Academics <ls_ac_dl(a)imperial.ac.uk>, DoLS Research Fellows <ls_refel_dl(a)imperial.ac.uk>, DoLS Research Staff <ls_re_dl(a)imperial.ac.uk> CC: Evans, Pat M <pat.evans(a)imperial.ac.uk>, Morris, Ian W <i.w.morris(a)imperial.ac.uk>, Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear All, You are invited to attend the seminars given by the shortlisted candidates who applied to the *Parasitology/Vector Biology position* advertised a couple of months ago. Date: *Thursday 27^th and Friday 28^th September 2012* Location: the Link Theatre, 2^nd floor, Sir Ernst Chain Building Thursday 27th 10am : Dr Dina Vlachou, Imperial College 10.30am: Dr Tony Nolan, Imperial College 11.00am: Dr Jake Baum, Walter & Eliza Hall Institute, Melbourne *Friday 28th* 10am : Dr Mara Lawniczak, Imperial College 10.30am: Dr Nikolai Windbichler, Imperial College 11.00am: Dr Ilaria Russo, University of Perugia Sandrine /Sandrine Nurboja/ /Department Operations Administrator/ /and PA to Professor Nick Franks/ /Cell and Molecular Biology Division/ /Biophysics Section/ /Imperial College/ /401 Sir Ernst Chain Building/ /London SW7 2AZ/ /Tel: 020 7594 7621/

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[FILM-Users 00258] Fwd: FW: Post-doc position Cell Biology/ Institut Pasteur
by Martin Spitaler 26 Sep '12

26 Sep '12

...forwarded for your information; as usual, the original attachment can be found on our documentation server (\\film-nas.med.ic.ac.uk\film-user mailing list\PostdocPosition.Pasteur-1.pdf), Martin text only version: Postdoctoral Position Membrane Traffic and Cell Division Laboratory Institut PASTEUR, Paris, FRANCE A funded postdoctoral position is currently available in the "Membrane Traffic and Cell Division laboratory" of Dr. Echard at the Institut Pasteur in Paris. This position has no nationality restriction and the working language is English. Our research focuses on the role of membrane trafficking in lipid and cytoskeleton remodeling at each step of animal cell division, under normal and pathological conditions. We are particularly interested in cytokinesis, the final step of cell division leading to the physical separation of the daughter cells. Using Drosophila and human cells, high-content RNAi-based screens, state-of-the-art live-cell imaging and advanced microscopy techniques, we are exploring the novel and promising interface between cytokinesis and membrane traffic at the cell and tissue level. Our recent work revealed unexpected connections between the human Lowe syndrome, endocytosis, cytoskeleton and cell division. We are seeking a highly motivated candidate for joining our dynamic and international team with: - a PhD and 0-3 years of relevant postdoctoral experience - a strong track-record of publications in peer-reviewed journals - validated experience in cell biology, RNAi-based screens, mouse models, lipid biology and/or cell imaging. Please send your application as a single PDF document containing a cover letter, a concise summary of previous research, a Curriculum Vitae, a publication list and contact details for at least 2 references to arnaud.echard(a)pasteur.fr For further information, please contact arnaud.echard(a)pasteur.fr and visit our website: http://www.pasteur.fr/ip/easysite/pasteur/en/research/scientific-department… units-and-groups/membrane-traffic-and-cell-division Selected Recent Publications: An ARF6/Rab35 GTPase Cascade for Endocytic Recycling and Successful Cytokinesis. Chesneau L, Dambournet D, Machicoane M, Kouranti I, Fukuda M, Goud B, Echard A. Current Biology: 22, 147-53 (2012) Rab35 GTPase and OCRL phosphatase remodel lipids and F-actin for successful cytokinesis. Dambournet D., Machicoane M., Chesneau L., Rocancourt M., Formstecher E., Salomon R., Goud B. and Echard A. Nature Cell Biology: 13, 981-8 (2011) Rab and actomyosin-dependent fission of transport vesicles at the Golgi complex. Miserey-Lenkei S, Chalancon G, Bardin S, Formstecher E, Goud B, Echard A. Nature Cell Biology: 12, 645-54 (2010) -------- Original Message -------- Subject: FW: Post-doc position Cell Biology/ Institut Pasteur Date: Mon, 24 Sep 2012 09:52:51 +0100 From: Moore, Peter J <peter.moore(a)imperial.ac.uk> Apologies if this is not relevant. *From:*Seabra, Miguel C *Sent:* 23 September 2012 20:03 *To:* Moore, Peter J *Subject:* Fwd: Could you forward to Imperial? / Post-doc position Cell Biology/ Institut Pasteur Begin forwarded message: *From: *Arnaud.Echard <arnaud.echard(a)pasteur.fr <mailto:arnaud.echard@pasteur.fr>> *Date: *21 September 2012 13:45:53 GMT+01:00 *To: *<m.seabra(a)imperial.ac.uk <mailto:m.seabra@imperial.ac.uk>> *Subject: Could you forward to Imperial? / Post-doc position Cell Biology/ Institut Pasteur* Dear Friends and Colleagues, Please find attached a PDF advertisement for a postdoctoral scientist position in my laboratory at the Institut PASTEUR, Paris, France. The aim is to understand the novel interface between membrane trafficking and cell division, both under normal and pathological conditions. I would be grateful if you could forward this to other colleagues and potential candidates, or have it posted in relevant places. Many thanks! Arnaud ECHARD -- Arnaud ECHARD, PhD. Group Leader Institut Pasteur Membrane Traffic and Cell Division G5 Lab. CNRS URA2582 28 rue du Dr. Roux 75015 Paris, France office: + 33 (0)1 44 38 94 09 lab: + 33 (0)1 44 38 94 08 fax: + 33 (0)1 45 68 89 54 secretary: + 33 (0)1 45 68 83 27 arnaud.echard(a)pasteur.fr <mailto:arnaud.echard@pasteur.fr> http://www.pasteur.fr/ip/easysite/pasteur/en/research/scientific-department…

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[FILM-Users 00256] Fwd: 23Nov/save the date!/Symposium Imaging Immune cells
by Martin Spitaler 21 Sep '12

21 Sep '12

...forwarded for your information; the attachment can be found on the FILM DOCUMENTATION SERVER under <...\film user mailing list\Imaging Immune cells 23 Nov 2012.pdf> (see FILM website /GUIDELINES / DATA for server details). Martin -------- Original Message -------- Subject: 23Nov/save the date!/Symposium Imaging Immune cells Date: Wed, 19 Sep 2012 09:18:20 +0100 From: Francois Lassailly <Francois.Lassailly(a)CANCER.ORG.UK> Reply-To: Francois Lassailly <Francois.Lassailly(a)CANCER.ORG.UK> To: <UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK> Dear colleagues, This is now 10 years* that in vivo intravital microscopy is being used in Immunology. In order to celebrate this anniversary, several international specialists will present recent developments during a special symposium organised on November 23^rd at Institut Cochin in Paris, focussing on the behaviour of immune cells withintumours and inflamed tissues (in human and mouse). For more information please see attached poster Inscription are free but required: _https://docs.google.com/spreadsheet/viewform?formkey=dFBkMDB6S1lpbUhFMlhDc… <https://docs.google.com/spreadsheet/viewform?formkey=dFBkMDB6S1lpbUhFMlhDcT…>_ __ *P. Bousso, N. R. Bhakta, R. S. Lewis, E. Robey, /Science/ 296,1876 (2002). *M. J. Miller, S. H. Wei, I. Parker, M. D. Cahalan, /Science/ 296, 1869 (2002). *S. Stoll, J. Delon, T. M. Brotz, R. N. Germain, /Science/ 296,1873 (2002) Subject: 23Nov/save the date!/Symposium Imaging Immune cells Chers collègues, On fête cette année les 10 ans* de l’utilisation de la *microscopie intravitale **/in vivo/** en immunologie*. Cette approche, permettant de *visualiser les cellules immunes* dans leur *environnement naturel*,a permis de faire des progrès très importants; de mettre en évidence des *comportements cellulaires totalement ignorés* jusque là. Citons notamment l’incroyable mobilité des lymphocytes T observée dans les ganglions lymphatiques de souris. Pour commémorer cet anniversaire, nous avons voulu faire le point sur l’avancement des recherches dans le domaine, en nous concentrant plus particulièrement sur le *comportement des cellules immunes dans les tumeurs et les tissus enflammés* (chez l'homme et la souris). Le *23 Novembre 2012*, nous aurons le privilège d’écouter plusieurs spécialistes français, européens et américains qui viendront à l'Institut exposer leurs résultats. Pour rappel, ce *symposium est gratuit* mais nécessite une inscription au préalable. Nous vous invitons à vous inscrire rapidement sur le site de l'Institut à l'adresse suivante : https://docs.google.com/spreadsheet/viewform?formkey=dFBkMDB6S1lpbUhFMlhDcT… En espérant vous voir nombreux le 23 Novembre. Bien amicalement, Nadège et Emmanuel *P. Bousso, N. R. Bhakta, R. S. Lewis, E. Robey, /Science/ 296, 1876 (2002). *M. J. Miller, S. H. Wei, I. Parker, M. D. Cahalan, /Science/ 296, 1869 (2002). *S. Stoll, J. Delon, T. M. Brotz, R. N. Germain, /Science/ 296, 1873 (2002) -- ____________________________________________________________ Emmanuel DONNADIEU Institut COCHIN INSERM U1016/CNRS UMR 8104/Université Paris Descartes 22, rue Méchain 75014 PARIS France Phone : +33 1 40516564 Fax : +33 1 40516555 email :emmanuel.donnadieu@inserm.fr ____________________________________________________________ NOTICE AND DISCLAIMER This e-mail (including any attachments) is intended for the above-named person(s). If you are not the intended recipient, notify the sender immediately, delete this email from your system and do not disclose or use for any purpose. We may monitor all incoming and outgoing emails in line with current legislation. We have taken steps to ensure that this email and attachments are free from any virus, but it remains your responsibility to ensure that viruses do not adversely affect you. Cancer Research UK Registered charity in England and Wales (1089464), Scotland (SC041666) and the Isle of Man (1103) A company limited by guarantee. Registered company in England and Wales (4325234) and the Isle of Man (5713F). Registered Office Address: Angel Building, 407 St John Street, London EC1V 4AD. -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT --

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[FILM-Users 00255] CO2 Incubator working again
by Rothery, Stephen M 18 Sep '12

18 Sep '12

The CO2 Incubator in room 409 is now working properly again Bw Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel +44-(0)207 594 9793 And ICTEM, (L-Block) Room 314 Hammersmith Campus Imperial College London Du Cane Road London, W12 0NN Tel. +44-(0)207 594 1699 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility **************************************************************

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[FILM-Users 00253] Fwd: Olympus BioScapes Competition
by Martin Spitaler 14 Sep '12

14 Sep '12

...forwarded, for your information, Martin -------- Original Message -------- Subject: Olympus BioScapes Competition Date: Wed, 12 Sep 2012 15:27:33 -0400 From: Michael W. Davidson <davidson(a)magnet.fsu.edu> To: <m.spitaler(a)imperial.ac.uk> Dear Dr. Spitaler, Each year, the BioScapes judges must pass over excellent images in order to narrow down the field into 10 winning entries. As a result, images that might otherwise be chosen by a different judging panel are dropped from the competition. As there will be a new panel this year, several of your previously entered images would be prime candidates for consideration. I hope you will be able to enter the 2012 Olympus BioScapes International Digital Imaging competition this year. If you are too busy or haven't had the opportunity to gather new images, let me know and I can transfer several of your previously entered images into this year's competition. The competition URL is: http://www.olympusbioscapes.com/enter.asp Please let me know if you require any assistance from us or experience difficulty with uploading images. Regards---Mike Michael W. Davidson National High Magnetic Field Laboratory 1800 E. Paul Dirac Dr. The Florida State University Tallahassee, FL 32310 Phone: 850-644-0542 Fax: 850-644-8920 Web: http://microscopy.fsu.edu

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[FILM-Users 00254] Fwd: Olympus BioScapes Competition
by Martin Spitaler 14 Sep '12

14 Sep '12

-------- Original Message -------- Subject: Olympus BioScapes Competition Date: Wed, 12 Sep 2012 15:27:33 -0400 From: Michael W. Davidson <davidson(a)magnet.fsu.edu> To: <m.spitaler(a)imperial.ac.uk> Dear Dr. Spitaler, Each year, the BioScapes judges must pass over excellent images in order to narrow down the field into 10 winning entries. As a result, images that might otherwise be chosen by a different judging panel are dropped from the competition. As there will be a new panel this year, several of your previously entered images would be prime candidates for consideration. I hope you will be able to enter the 2012 Olympus BioScapes International Digital Imaging competition this year. If you are too busy or haven't had the opportunity to gather new images, let me know and I can transfer several of your previously entered images into this year's competition. The competition URL is: http://www.olympusbioscapes.com/enter.asp Please let me know if you require any assistance from us or experience difficulty with uploading images. Regards---Mike Michael W. Davidson National High Magnetic Field Laboratory 1800 E. Paul Dirac Dr. The Florida State University Tallahassee, FL 32310 Phone: 850-644-0542 Fax: 850-644-8920 Web: http://microscopy.fsu.edu

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[FILM-Users 00252] Fwd: quartz bottom plate
by Martin Spitaler 14 Sep '12

14 Sep '12

...forwarded request - can anybody help? Martin -------- Original Message -------- Subject: quartz bottom plate Date: Fri, 14 Sep 2012 15:24:13 +0100 From: Liebeke, Manuel <m.liebeke(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi, does anyone has a quartz bottom 96 well plate I could test for an UV assay? With kind regards Dr. Manuel Liebeke Biomolecular Medicine Department of Surgery and Cancer Faculty of Medicine Imperial College London London, UK SW7 2AZ Tel. +44 207594 3231 http://www1.imperial.ac.uk/medicine/divisions/surgeryandcancer/ http://www1.imperial.ac.uk/medicine/people/m.liebeke/

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[FILM-Users 00251] Fwd: Imperial Flow Cytometry Course 2012
by Martin Spitaler 14 Sep '12

14 Sep '12

...forwarded for your information; it's short notice (I've only just received the email), but it should be very interesting for many microscope users - FACS and microscopy in combination are extremely powerful! Martin -------- Original Message -------- Date: Fri, 14 Sep 2012 12:56:00 +0100 From: Poobalasingam, Thurka <t.poobalasingam(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Imperial core flow cytometry facility staff are pleased to offer this intensive, two day educational course in the varied techniques using flow cytometry. The course will initially cover basic flow cytometry but then rapidly move on to the more advanced applications providing you with vital information on important cutting-edge topics in spectral overlap and compensation issues in flow cytometry, staining and sample prep, calibration issues, as well as covering Imagestream technology and its applications, as well covering various multi-plexing techniques. These will also include cell cycle, apoptosis, cell proliferation, and fluorescent proteins and FRET and covering topics such as looking for immune patterns in MS. Please check our programme for confirmed speakers and talks. (http://www1.imperial.ac.uk/nhli/respiratory/respinfect/flow/imperial_flow_c…) The course has been designed to allow the participant to gain experience of what a flow cytometer is capable of as well as to raise awareness of the problems that can be encountered when setting up polychromatic panels. No prior knowledge of flow cytometry is assumed. This course will give you a firm grounding in the basics of flow cytometry, as well as delving into the complicated issues researchers can often face. Registration closes on the 18th as I then have to give catering numbers etc. *Thurka Poobalasingam* * * *Flow Cytometry Technician* *Respiratory Infections Section* *Respiratory Sciences * *National Heart & Lung Institute (NHLI)* *Imperial College London* *Room 312D, Third floor FACS Facility, * *Medical School Building* *St. Mary's Campus* *Norfolk Place* *Paddington* *London W2 1PG* *Tel: 020 7594 3763* *Email: t.poobalasingam(a)imperial.ac.uk* *Website: * *http://www1.imperial.ac.uk/medicine/about/institutes/centre_respiratory_infection/flow/* ------------------------------------------------------------------------ *From:* Spitaler, Martin *Sent:* 14 September 2012 11:21 *To:* Poobalasingam, Thurka *Subject:* Re: FW: Request for Abstract, and copy of powerpoint talk for flow course Thurka, preparing my talk I've just notice I only have a very preliminary programme - do you have a final version? And is your course fully booked? I guess yes, but if needed, I can send an invitation to our users. Best, Martin On 04/09/2012 23:41, Poobalasingam, Thurka wrote: > I hope you do not mind, but I used the links you have on your site to > speed things up a bit at my end, but its done and goes live once > somebody (somewhere higher up) approves the page. > Thanks again Martin for the nudge. > BW > > *Thurka Poobalasingam* > * > * > *Flow Cytometry Technician* > > *Respiratory Infections Section* > > *Respiratory Sciences * > > *National Heart & Lung Institute (NHLI)* > > *Imperial College London* > > *Room 312D, Third floor FACS Facility, * > > *Medical School Building* > > *St. Mary's Campus* > > *Norfolk Place* > > *Paddington* > > *London W2 1PG* > > *Tel: 020 7594 3763* > > *Email: t.poobalasingam(a)imperial.ac.uk* > > *Website: > * > > *http://www1.imperial.ac.uk/medicine/about/institutes/centre_respiratory_infection/flow/* > > > ------------------------------------------------------------------------ > *From:* Spitaler, Martin > *Sent:* 04 September 2012 14:33 > *To:* Poobalasingam, Thurka > *Subject:* Re: FW: Request for Abstract, and copy of powerpoint talk > for flow course > > PS: given that microscopy and FACS are often used in combination, it > would be useful if you added the link to the FILM website to your own > website (yours is already on ours > <http://www3.imperial.ac.uk/imagingfacility/links/core_facilities>) > > > On 28/08/2012 11:43, Poobalasingam, Thurka wrote: >> Hi Martin, >> >> I'm happy to take just an abstract for now to advertise the course a >> bit more as well as the booklets I need to prepare....the talk itself >> was to prepare booklets in advance to hand-out on the mornings during >> registration. >> >> Many thanks >> >> *Thurka Poobalasingam* >> * >> * >> *Flow Cytometry Technician* >> >> *Respiratory Infections Section* >> >> *Respiratory Sciences * >> >> *National Heart & Lung Institute (NHLI)* >> >> *Imperial College London* >> >> *Room 312D, Third floor FACS Facility, * >> >> *Medical School Building* >> >> *St. Mary's Campus* >> >> *Norfolk Place* >> >> *Paddington* >> >> *London W2 1PG* >> >> *Tel: 020 7594 3763* >> >> *Email: t.poobalasingam(a)imperial.ac.uk* >> >> *Website: >> * >> >> *http://www1.imperial.ac.uk/medicine/about/institutes/centre_respiratory_infection/flow/* >> >> >> ------------------------------------------------------------------------ >> *From:* Spitaler, Martin >> *Sent:* 28 August 2012 10:35 >> *To:* Poobalasingam, Thurka >> *Subject:* Re: FW: Request for Abstract, and copy of powerpoint talk >> for flow course >> >> Dear Thurka, >> >> I don't think I will be able to submit my talk yet, too many other >> things are more urgent at the moment. I'll try to do it as soon as >> possible, but I very much doubt many participants will look at it so >> early anyway. >> >> Best regards, >> >> Martin >> >> On 23/08/2012 16:42, Poobalasingam, Thurka wrote: >>> >>> Dear Invited Speaker >>> >>> I am writing to invite you to submit a short abstract and to >>> finalise the title of your intended talk (if not done so already). >>> >>> In late August/early September I am hoping all Speakers will be able >>> to provide a copy of their proposed talk; I am aiming to have >>> booklets printed to be ready for the second week of September. This >>> has arisen due to comments received from previous courses >>> of delegates requesting printouts of presentations to be used as >>> reference material during the talks. >>> >>> I would be happy for speakers to obviously omit anything they do not >>> wish to be printed and I acknowledge that talks are even amended at >>> the last hour, so having the backbone of the slides to be presented, >>> would be very helpful and very much appreciated. >>> >>> Please feel free to contact me should you need any further >>> information and I look forward to hearing from you. >>> >>> Best wishes, >>> >>> **Thurka* Poobalasingam* >>> >>> *Flow Cytometry Technician* >>> >>> *Respiratory Infections Section* >>> >>> *Respiratory Sciences * >>> >>> *National Heart & Lung Institute (NHLI)* >>> >>> *Imperial College London* >>> >>> *Room 312D, Third floor FACS Facility, * >>> >>> *Medical School Building* >>> >>> *St. Mary's Campus* >>> >>> *Norfolk Place* >>> >>> *Paddington* >>> >>> *London W2 1PG* >>> >>> *Tel: 020 7594 3763* >>> >>> *Email: t.poobalasingam(a)imperial.ac.uk >>> <mailto:t.poobalasingam@imperial.ac.uk>* >>> >>> *Website: * >>> >>> *http://www1.imperial.ac.uk/medicine/about/institutes/centre_respiratory_infection/flow/* >>> >> >

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[FILM-Users 00250] Definiens software news
by Martin Spitaler 13 Sep '12

13 Sep '12

Dear microscopists, as you might know, we have the very powerful Definiens image analysis software in the facility, and we keep using it constantly for a range of demanding image analysis tasks. Some of you might not be aware of the advantages this might have for your own microscopy projects. We are therefore planning to re-instate our monthly DEFINIENS USER GROUP meeting, where we present current and past solutions to common (or more exotic) image analysis challenges. Please let me know if you are interested, so we can arrange place, date and time of the user group meeting. And for your information, the online DEFINIENS FORUM and RULESET EXCHANGE have become quite lively recently, and now they contain a long list of useful Tips, Tricks, ideas and downloadable rulesets. Some generally interesting pages should be: USER FORUM: <http://community.definiens.com/user-forum.html>, with topics from beginners to various expert tasks RULESET EXCHANGE: <http://community.definiens.com/rule-set-exchange.html>, a long list of downloadable rulesets to solve common problems; examples are: # Detection and Quantification of 3D Signals in Confocal Images # Segmentation of a line via layer arithmetics # Rolling Ball background correction The FILM Crew is happy to get you started with a basic trial analysis of your data and a personal introduction. We also have some capacity to do analysis tasks for users, although for bigger projects, we would need to plan this well in advance. Good imaging, Martin <http://www3.imperial.ac.uk/imagingfacility/equipment/licence_definiens> -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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