July 2012 - FILM-Users - imperial.ac.uk

[FILM-Users 00239] FW: FILM registration confirmation
by Rothery, Stephen M 18 Jul '12

18 Jul '12

Hi All Does anyone have any experience using luciferase (luc2 in particular) to measure cell viability? would welcome any advice Thanks so much, Allie Alessondra Speidel a.speidel11(a)imperial.ac.uk<mailto:a.speidel11@imperial.ac.uk>

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[FILM-Users 00238] FW: FLIPR ?
by Rothery, Stephen M 18 Jul '12

18 Jul '12

Can anyone help Eric bw Steve Hi, Would you know where I could have access to a FLIPR system in SAF bld ? (to assess calcium signals) Cheers Eric Dr Eric Dubuis Tel: + 44 (0) 207 594 8427 Fax: + 44 (0) 207 594 3100 Research Fellow in Airway Disease National Heart and Lung Institute Faculty of Medicine Sir Alexander Fleming Building South Kensington Campus

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[FILM-Users 00237] Special Seminar
by Rothery, Stephen M 18 Jul '12

18 Jul '12

TODAY Special Seminar Dr Omar Qureshi University of Birmingham "Understanding trans-endocytosis in immune regulation; how to rip stimulatory molecules from antigen-presenting cells" 12.30 pm Wednesday July 18th Link Lecture Theatre (290) Sir Ernst Chain building (formerly Biochemistry) South Kensington Campus All welcome Host: Murray Selkirk

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[FILM-Users 00236] FILM staff away
by Martin Spitaler 10 Jul '12

10 Jul '12

Dear microscopists, in the coming weeks, we will take turns to go away on meetings and summer holidays, starting with myself from tomorrow afternoon. This means that until mid August, the remaining members of the FILM crew might be a bit stretched when it comes to training and support. We will certainly do our best to respond to your needs as quickly as possible, but please be patient when things take a bit longer than usual to be sorted. I know many of you will be away as well, so it should hopefully work out well. Afterwards, we will be back in business refreshed by hopefully lots of sunshine (hopefully also Steve in Scotland). Good wishes for your own trips or holidays, and good imaging while here, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00235] Fwd: RE: Volocity
by Martin Spitaler 10 Jul '12

10 Jul '12

...forwarded for your information; I won't have time, but maybe someone else is interested in being invited by Perkin Elmer to give a presentation about using Volocity for quantification at the European Microscopy Congress in Manchester in September, Martin -------- Original Message -------- Subject: RE: Volocity Date: Tue, 10 Jul 2012 13:22:12 +0000 From: Barlow, Andrew <Andrew.Barlow(a)PERKINELMER.COM> To: Martin Spitaler <m.spitaler(a)imperial.ac.uk> On 09/07/2012 12:22, Barlow, Andrew wrote: Dear Martin. My name is Andrew Barlow, I'm an application specialist working on Volocity. I understand that you co-ordinate a number of Volocity systems. I'm currently searching for a user of Volocity quantitation that might be prepared to give a presentation at a PerkinElmer workshop at the European Microscopy Congress in Manchester. Would you be able to suggest anybody that might be interested? PE would be able to offer travel and accommodation expenses for speakers. I'm also interested to hear how your users are progressing with the new Quantitation features added in Volocity 6. Do you feel that they would benefit from a visit from us for some training and advice? Regards, Andrew ** Andrew Barlow PhD | Applications Specialist PerkinElmer | For the Better Andrew.Barlow(a)perkinelmer.com <mailto:Andrew.Barlow@perkinelmer.com> Phone: +44 2476 692229| Fax: +44 2476 690091 | Mobile: +44 7799 795 999 Millburn Hill Road, Coventry, CV4 7HS, UK www.perkinelmer.com <http://www.perkinelmer.com> *Please consider the environment before printing this e-mail.* This e-mail message and any attachments are confidential and proprietary to PerkinElmer, Inc. If you are not the intended recipient of this message, please inform the sender by replying to this email or sending a message to the sender and destroy the message and any attachments. Thank you.

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[FILM-Users 00234] New Huygens deconvolution software
by Martin Spitaler 05 Jul '12

05 Jul '12

Dear microscopists, as part of the equipment purchase for the L-Block (NHLI) in Hammersmith, we have also got a licence for the powerful deconvolution software SVI Huygens, including modules for CONFOCAL DECONVOLUTION and MULTIPHOTON DECONVOLUTION. Advantages over our existing Autoquant deconvolution: # additional modules for confocal and multiphoton deconvolution # more control over the deconvolution settings # centralised installation, accessible from everywhere in the college # better batch processing (including line command option) The software is installed on a server of the Bioinformatics Support Service, so it can be used from anywhere in the college. At the moment we have 2 concurrent user licences, so 2 people can use the software in parallel at any time. Prioritisation is via the FILM HUYGENS booking page on Sharepoint. To get access, please email to set up your access and give you all required information. Charging is at our normal software charging rate, see FILM website / GUIDELINES / CHARGES for the latest rates (according to automated usage logs). If you want to use the software, please get in touch. Good imaging, Martin PS: thanks all of you who came along and contributed to the success of the Microscopy Day, it's been a very productive event, with lots of discussions, and the presentations of the Matchmaker session and posters were very interesting -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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