May 2012 - FILM-Users - imperial.ac.uk

[FILM-Users 00209] Fwd: Electron Microscopy and Tomography Position - University of Exeter
by Martin Spitaler 16 May '12

16 May '12

...forwarded, for your information, Martin ================================================= -------- Original Message -------- Subject: Electron Microscopy and Tomography Position - University of Exeter Date: Tue, 15 May 2012 12:22:07 +0100 From: Paszkiewicz, Konrad <K.H.Paszkiewicz(a)EXETER.AC.UK> Reply-To: Paszkiewicz, Konrad <K.H.Paszkiewicz(a)EXETER.AC.UK> To: <UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK> Dear all, Could you please circulate this to any interested colleagues? This full time post is primarily to support the Exeter Bio-Imaging Centre, as part of a new Biomedical Informatics Hub, which is funded by a recent Wellcome Trust Institutional Strategic Support Fund award. This Biomedical Informatics Hub will provide informatics support to a range of emerging technologies and support multiple research areas. The post holder will carry out research activities and support grant and income generation related to Wellcome Trust projects. The post holder will provide support to cell biologists studying fundamental aspects of cellular organisation, membrane trafficking, the cytoskeleton, and microbial pathogenesis. We are particularly interested in applications from candidates with expertise in advanced electron microscopy and related computational techniques (including tomography and 3D reconstruction of ultra-structural data from biological specimens; reconstruction of single molecule images). Candidates should have expertise in all aspects of electron microscopy sample preparation, immunogold labelling, usage and running of an electron microscopes, and computational and image analysis experience. The post holder will personally undertake sample preparation, perform imaging and analysis on behalf of researchers and as part of collaborative research projects, and will be expected to contribute to research papers and grant proposals as required. For job particulars please see http://www.admin.ex.ac.uk/personnel/jobs/R11159.pdf and apply online at https://jobs.exeter.ac.uk For further information contact Professor Gero Steinberg, e-mail G.Steinberg(a)exeter.ac.uk or telephone (01392) 723476. Dr Konrad Paszkiewicz Head, Exeter Sequencing Service, Biosciences, Stocker Road, University of Exeter, Exeter EX4 4QD, UK. http://biosciences.exeter.ac.uk/facilities/sequencing/ http://biosciences.exeter.ac.uk/facilities/exbiss/ -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT --

1 0

[FILM-Users 00208] Fwd: [neuroscience-technology-network] Connectomics Seminar - Professor George Augustine
by Martin Spitaler 16 May '12

16 May '12

...forwarded, for your information, Martin ====================================================================================== -------- Original Message -------- Subject: [neuroscience-technology-network] Connectomics Seminar - Professor George Augustine Date: Mon, 14 May 2012 15:19:14 +0000 From: Fenton, Rob J <r.fenton(a)imperial.ac.uk> To: neuroscience-technology-network <neuroscience-technology-network(a)imperial.ac.uk> Dear Colleague, Please note below the forthcoming lecture that may be of interest to you. Professor George Augustine 'Optogenetic mapping of brain circuitry' 4pm in RSM 2.28 on Monday 21st May Biography: Prof. Augustine is a distinguished neurobiologist and a Faculty Professor at Duke-NUS Medical School in Singapore. He is also Director of the new Center for Functional Connectomics at the Korea Institute of Science and Technology. He has broad interests in cellular and circuit neuroscience, and will talk about his recent work using novel optogenetic tools to map the fine organisation of nervous system I am in process of drawing up a schedule for individual meetings; please let me know if you'd like to meet with Prof. Augustine Thanks, Paul Chadderton ____________________________ Paul Chadderton Department of Bioengineering Imperial College London Prince Consort Road London SW7 2BW T: +44-20-7594-0770 E: p.chadderton(a)imperial.ac.uk <mailto:p.chadderton@imperial.ac.uk>

1 0

[FILM-Users 00207] Fwd: anyone anti-mCherry antibody for use in western blot?
by Martin Spitaler 11 May '12

11 May '12

Can anybody help? Martin -------- Original Message -------- Subject: anyone anti-mCherry antibody for use in western blot? Date: Thu, 10 May 2012 11:16:46 +0100 From: Semplici, Francesca <f.semplici(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, I am looking for an anti-mCherry antibody that I could borrow (a few microliters for a western blot) if anyone has some. Could you please be so kind to circulate my request to FILM users? Thank you so much indeed. Best wishes, Francesca

1 0

[FILM-Users 00206] Black cardigan
by Rothery, Stephen M 11 May '12

11 May '12

Hi all A small black cardigan (ladies medium) has been left in the widefield room. It has been there for a few weeks/months now If anyone has missed it, it is now hanging on my desk (403). Please come and collect Bw Steve FILM - Facility for Imaging by Light Microscopy ************************************************************** Steve Rothery FILM Technician Sir Alexander Fleming Building, desk 403 Imperial College London / South Kensington Exhibition Road London SW7 2AZ Tel. +44-(0)207 594 9793 E-mail s.rothery(a)imperial.ac.uk<mailto:s.rothery@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility **************************************************************

1 0

[FILM-Users 00205] FILM server down
by Martin Spitaler 10 May '12

10 May '12

Dear microscopists, unfortunately the FILM server is down at the moment, ICT have been contacted and will hopefully get it back up and running again soon. Sorry for the troubles, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 0

[FILM-Users 00204] FILM server down
by Martin Spitaler 10 May '12

10 May '12

Dear microscopists, unfortunately the FILM server is down at the moment, ICT has been contacted and will hopefully get it back up and running again soon. Sorry for the troubles, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 0

[FILM-Users 00203] FILM Microscopy Day 2012
by Martin Spitaler 10 May '12

10 May '12

Dear microscopists, on FRIDAY 29 JUNE we will have the FILM MICROSCOPY DAY 2012 - as in previous years, a day full of talks on all aspects of light microscopy, from the very basics to very advanced technologies (in vivo imaging, super-resolution, non-linear techniques). The presentations will be by FILM staff (Steve, Mark and myself) supported by two experts in their field, VALENTINA CAORSI (NHLI) on non-linear fluorescence techniques (fluorescence lifetime imaging and its various applications) and LUIS PIZARRO (COMPUTING) on image analysis. We also have a special novelty: To make people aware what's going on around them and stimulate new collaborations, we will have a MICROSCOPY MATCH MAKER, where you FILM users are all invited to present your exciting research projects in a short snapshot oral presentation and / or a poster. Oral presentations are strictly limited to a MAXIMUM OF 2 MINUTES / 2 POWERPOINT SLIDES - longer discussions will be possible at the posters over lunchtime. An excellent opportunity to initiate new collaborations, don't miss out - PIs, please encourage your students and postdocs to participate. Please REGISTER BY FRIDAY 1 JUNE by email to Ruth O'Donnell <r.odonnell(a)imperial.ac.uk>, including your MATCHMAKER OPTIONS (presentation, poster, none). >>PROGRAMME<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< 10.00- 10.10 Welcome by Prof Tony Magee, head of FILM GENERAL MICROSCOPY TECHNIQUES 10.10-10.55 Basics of microscopy - magnification, properties of light, resolution, transmitted light contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy (Martin Spitaler, FILM) 10.55-11.40 Live imaging techniques - Multiphoton, live imaging and second harmonic generation (SHG) (Mark Scott, FILM) 11.40-12.00 coffee & tea break 12.000-13.00 MICROSCOPY MATCHMAKER: FILM users giving a snapshot of their work in 2-minute presentations 13.00-14.15 Poster session with sandwich lunch (kindly sponsored by CARL ZEISS MICROSCOPY) SPECIAL TECHNIQUES 14.15-15.00 Fluorophores as sensors - colocalisation, fluorescence lifetime (FLIM) and resonant energy transfer (FRET) (Valentina Caorsi, NHLI) 15.00-15.45 Super-resolution microscopy - TIRF, PALM, STORM (Steve Rothery, FILM) 15.45-16.05 coffee & tea break UNDERSTANDING IMAGES 16.05-16.50 Understanding and visualisation of image data: data formats, pixel and metadata, image visualisation and presentation, OMERO database (Martin Spitaler, FILM) 16.50-17.35 Image analysis: noise removal, deconvolution, segmentation, tracking, registration (Luis Pizarro, Computing) 17.35-17.40 Wrap-up, feedback, outlook For more and up-to-date information, please check <http://www3.imperial.ac.uk/portal/page/portallive/imagingfacility/news>. A flyer is also available <https://workspace.imperial.ac.uk/imagingfacility/Public/FILM%20Microcopy%20…> See you all there. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 0

[FILM-Users 00202] Fwd: Definiens Symposium 2012 in München
by Martin Spitaler 04 May '12

04 May '12

...forwarded, for your information; should be an interesting meeting, Martin -------- Original Message -------- Subject: Definiens Symposium 2012 in München Date: Fri, 4 May 2012 17:47:12 +0200 From: Zimmermann, Johannes <jzimmermann(a)definiens.com> To: <m.spitaler(a)imperial.ac.uk> CC: Huber, Monika <mhuber(a)definiens.com> Hello Martin, I regret very much not seeing you in Munich at the Symposium in June (14-16). Maybe the event (especially the workshops) would be interesting for people in your group as well. As discussed, please find below some additional information: Symposium Page: http://www.definiens.com/community/symposium.html Program: http://www.definiens.com/community/symposium/symposium-2012-program.html Workshops: http://www.definiens.com/community/symposium/symposium-2012-workshops.html Registration: http://info.definiens.com/2012Symposium_RegistrationPage.html Please let me know if you have further questions. Kind regards Johannes cid:image001.jpg@01CD02D1.DBAE0E10 ------------------------------------------------------------------------ *Dr. Johannes Zimmermann* Director Business Development Life Sciences Definiens AG Bernhard-Wicki-Str. 5 80636 München Germany Tel. +49 (89) 231180 32 Fax. +49 (89) 231180 90 Mob. +49 (162) 2665580 jzimmermann(a)definiens.com <mailto:jzimmermann@definiens.com> http://www.definiens.com <http://www.definiens.com/> cid:image002.png@01CD02D1.DBAE0E10 <http://www.definiens.com/community/symposium.html> ------------------------------------------------------------------------ *Deeper Insights - Faster Results - Better Decisions* ------------------------------------------------------------------------ Sitz der Gesellschaft/Registered Office: Munich, Germany; Vorstand/Executive Board: Thomas Heydler (Vorsitzender/CEO), Dr. Markus Rinecker; Vorsitzender des Aufsichtsrats/Chairman of the Supervisory Board: Friedrich Bornikoel Registergericht/Commercial Register München HRB 133088 This e-mail may contain trade secrets or privileged, undisclosed, or otherwise confidential information. If you have received this e-mail in error, you are hereby notified that any review, copying, or distribution of it is strictly prohibited. Please inform us immediately and destroy the original transmittal. Thank you for your cooperation. ------------------------------------------------------------------------ /IMPORTANT: The contents of this email and any attachments are confidential. They are intended for the named recipient(s) only. If you have received this email in error, please notify the system manager or the sender immediately and do not disclose the contents to anyone or make copies thereof. /**** eSafe scanned this email for viruses, vandals, and malicious content. **** ------------------------------------------------------------------------

1 0

[FILM-Users 00201] Fwd: CONFOCALMICROSCOPY: Leica web-seminar : , , Live Cell Imaging: Cell-Cell Interactions"
by Martin Spitaler 03 May '12

03 May '12

...forwarded, for your information, Martin -------- Original Message -------- Subject: CONFOCALMICROSCOPY Index - 1 May 2012 to 2 May 2012 (#2012-105) Date: Thu, 3 May 2012 00:06:28 -0500 From: CONFOCALMICROSCOPY automatic digest system <LISTSERV(a)LISTS.UMN.EDU> To: <CONFOCALMICROSCOPY(a)LISTS.UMN.EDU> CONFOCALMICROSCOPY Index - 1 May 2012 to 2 May 2012 (#2012-105) **Commercial Posting** Leica has organized a web-seminar in May that may be of interest to you: Live Cell Imaging: Cell-Cell Interactions Date: 24th May 2012 Time: 12:00pm ET Duration: 60 minutes http://mediazone.brighttalk.com/event/ReedElsevier/95e1533eb1-6086-intro [Please note that if the url above gets cut onto the next line, you will need to merge it] Speakers: Jeff Lichtman Professor of Molecular and Cellular Biology, Harvard University Quentin Sattentau Professor of Immunology, University of Oxford Dr. Ellen Robey Professor of Immunology and Pathogenesis, University of California, Berkeley In this interactive webinar, you will hear from renowned scientists who use live- cell imaging to study not only how cell-cell communication facilitates neuronal and immune cell interactions, but also how these modes of communication can be altered in the context of infection to promote pathogen transmission. Very best regards, Lon Nelson Director of Marketing Management Leica Microsystems, Inc. Living up to Life (847) 317-7218 (800) 248-0123 lon.nelson(a)leica-microsystems.com http://www.leica-microsystems.com/

1 0