February 2012 - FILM-Users

[FILM-Users 00174] Fwd: CFP and E2-Crimson fluorescent proteins
by Martin Spitaler 28 Feb '12

28 Feb '12

...forwarded on behalf of Elisa, can anybody help? Please reply to Elisa directly and NOT to the mailing list, Martin -------- Original Message -------- Subject: CFP and E2-Crimson fluorescent proteins Date: Tue, 28 Feb 2012 09:14:59 +0000 From: Belian, Elisa <e.belian09(a)imperial.ac.uk> To: Martin Spitaler <m.spitaler(a)imperial.ac.uk> Dear Martin, I was wondering if you know anyone who has plasmids expressing CFP and/ or E2-Crimson and could give me some DNA or bacterial stocks of those plasmids. I want to express those proteins in mammalian cells, so an expression vector would be ideal, but otherwise any vector will do. Thank you for circulating the email. Best, Elisa *Elisa Belian* * * Cardiac Myogenesis, Death and Regeneration National Heart and Lung Institute Faculty of Medicine Imperial College London 2nd Floor Sir Alexander Fleming Building Exhibition Road SW7 2AZ London United Kingdom E-mail: e.belian09(a)imperial.ac.uk <mailto:e.belian09@imperial.ac.uk>

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[FILM-Users 00173] TODAY: FILM Club on sample fixation
by Martin Spitaler 24 Feb '12

24 Feb '12

TODAY: Dear microscopists, in this Friday's FILM Club we will try something new: Some kind of technical mini-workshop on sample preparation and fixed versus live imaging. This will be based on the following recent publication, which I will summarise in the FILM Club: Immunolabeling artifacts and the need for live-cell imaging U Schnell, F Dijk, KA Sjollema & BNG Giepmans Nature Methods 9,152158(2012) <http://www.nature.com/nmeth/journal/v9/n2/full/nmeth.1855.html> The FILM Club will be held this Friday 24 Feb 2012 at 1pm in SAFB-120 (Fleming Building, South Kensington) See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00172] Fwd: FW: Perkin Elmer OPERETTA SYSTEM - please circulate
by Martin Spitaler 22 Feb '12

22 Feb '12

...forwarded for your information, Martin -------- Original Message -------- Subject: FW: Perkin Elmer OPERETTA SYSTEM - please circulate Date: Tue, 21 Feb 2012 15:57:10 +0000 From: Hipwell, Mandy A <m.hipwell(a)imperial.ac.uk> *From:*Ettorre, Anna *Sent:* 21 February 2012 15:40 *To:* Lee, Matt J; Boyton, Rosemary J; Altmann, Danny M; Friedland, Jon S; Muraro, Paolo A; Wojciak-Stothard, Beata; Karadimitris, Anastasios; Franzoso, Guido; Archanco, Marta; Mazarakis, Nicholas D B.Sc, Ph.D, FSB *Cc:* Jones, Amanda; 'kate.harper(a)perkinelmer.com'; Konitsiotis, Antonios D; Elkington, Paul T G; Purbhoo, Marco *Subject:* Perkin Elmer OPERETTA SYSTEM - please circulate Dear colleagues, Perkin Elmer has agreed to bring their High Content Imaging OPERETTA SYSTEM (http://www.perkinelmer.com/pages/020/cellularimaging/products/operetta.xhtml ) for a week demo to our BRC Facility at Hammersmith Campus (http://www1.imperial.ac.uk/departmentofmedicine/about/brc_imaging_and_facs_… ). The week demo will start on March 6^th until March 8^th . For those who are interested in having only a general overview of this High Content Imaging system, we will host a seminar on March 6^th , Wolfson Seminar Centre, seminar room 4, h: 11-12. Anybody who wants to try using his/her own cells is welcome to come. You can book a slot either by contacting directly Amanda Jones (*amanda.jones(a)perkinelmer.com <mailto:amanda.jones@perkinelmer.com>*) or by emailing myself. If you would like to have more info, please do not hesitate to contact me. Hope to meet you there. Best regards, Anna Ettorre Anna Ettorre, Ph.D. Research Fellow Faculty of Medicine Department of Medicine Section of Infectious Diseases and Immunity Commonwealth Building Hammersmith Campus Imperial College London DuCane Road London W12 0NN Tel: +44 (0)208 383 1284 Fax: +44 (0)20 8383 3394 e-mail: a.ettorre(a)imperial.ac.uk <mailto:a.ettorre@imperial.ac.uk>

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[FILM-Users 00171] FILM Club Fri 24 Feb 2011: sample preparation
by Martin Spitaler 22 Feb '12

22 Feb '12

Dear microscopists, in this Friday's FILM Club we will try something new: Some kind of technical mini-workshop on sample preparation and fixed versus live imaging. This will be based on the following recent publication, which I will summarise in the FILM Club: Immunolabeling artifacts and the need for live-cell imaging U Schnell, F Dijk, KA Sjollema & BNG Giepmans Nature Methods 9,152–158(2012) <http://www.nature.com/nmeth/journal/v9/n2/full/nmeth.1855.html> The FILM Club will be held this Friday 24 Feb 2012 at 1pm in SAFB-120 (Fleming Building, South Kensington) See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00169] OMERO and Definiens server down
by Martin Spitaler 16 Feb '12

16 Feb '12

Dear microscopists, unfortunately the server hosting Omero and Definiens is down at the moment, some unexpected technical fault. BSS are working on it, hopefully restarting will do the job and it will be back up soon again. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00168] Fwd: Operetta demo and HCS workshop
by Martin Spitaler 15 Feb '12

15 Feb '12

...forwarded, for your information, Martin -------- Original Message -------- Subject: Operetta demo and HCS workshop Date: Wed, 15 Feb 2012 15:08:44 +0000 From: Konitsiotis, Antonios D <antonios.konitsiotis05(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin, Can you please forward this to the FILM group? Thanks!! Following the visit from Perkin-Elmer in November we have been organising a date and place to demo the High content screening capabilities of the Operetta machine, as well as the Columbus software. With the help of Anna Ettorre from the BRC imaging and FACs facility we now have the dates and place sorted and I am collecting names for people from the South Kensington groups that would like to demo the machine and see if it is suitable for the work they are doing. The details of the workshop are as follows: Date: *6-8th March 2012* Place: *Imperial College Hammersmith Campus* Tuesday 6th March: *11:00 – 12:00 HCS Seminar (open to all)* @ The Wolfson Centre, Seminar Room 4, Hammersmith Campus. What is High Contents Screening? with Hans Pirard from PerkinElmer HCS instruments for everyone: Opera & Operetta/Harmony & Phenologic/Columbus Image Data Management. Applications with HCS *HCS Workshops* @ BRC Imaging and FACS Facility, Room 8.N.4, 8th Floor, Infectious Diseases and Immunity, Commonwealth Building, Hammersmith Campus *Tuesday 6th March*: *14:00 – 16:00 Pre-Bookable HCS Workshop * *Wednesday 7th March*: *10:00 – 13:00 Pre-Bookable HCS Workshop* *14:00 – 16:00* *Pre-Bookable HCS Workshop* *Thursday 8th March*: *10:00 – 13:00 - Pre-Bookable HCS Workshop* If anyone is interested in booking a slot to demo the machine please e-mail me (*antonios.konitsiotis05(a)imperial.ac.uk*) or e-mail (*amanda.jones(a)perkinelmer.com)*with the date and slot you prefer. All the best, Antonio */Antonio Konitsiotis/* Post-Doctoral Research Associate National Heart and Lung Institute, Division of Molecular Medicine, Sir Alexander Flemming Building, Imperial College South Kensington Campus London, SW7 2AZ UK

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[FILM-Users 00167] Fwd: FW: [High-Throughput-Technology-Community] High Throughput/High Content Technology Community
by Martin Spitaler 10 Feb '12

10 Feb '12

...forwarded, for your information, Martin -------- Original Message -------- Subject: FW: [High-Throughput-Technology-Community] High Throughput/High Content Technology Community Date: Thu, 9 Feb 2012 08:03:08 +0000 From: Sharp, Emma L <e.sharp(a)imperial.ac.uk> Dear All, Please see the note below from Rob Fenton for your information. Best wishes, Emma *From:*high-throughput-technology-community-bounces@imperial.ac.uk <mailto:high-throughput-technology-community-bounces@imperial.ac.uk> [mailto:high-throughput-technology-community-bounces@imperial.ac.uk] <mailto:[mailto:high-throughput-technology-community-bounces@imperial.ac.uk]> *On Behalf Of *Fenton, Rob J *Sent:* 08 February 2012 19:26 *To:* high-throughput-technology-community; Trim, Kimberley; Wilson, Rebecca K; Sri-Pathmanathan, Anusha A; Ip, Molly M L; Kerr, Colin; O' Neill, Anne; Oakeshott, John J; Reilly, John B; Spargo, Sheena; Tebbutt, Andrew C; Venables, Tim; Anderson, Diana R; Boyce, Eileen S; Evans, Pat M; Gayer, Gosia; Girard, Francis; Gray, Sandy E; Jones, Linda D; Jones, Richard L; Lyle, Karen R; Nurboja, Sandrine A C; Skene, Barbara; Tortelli, Maria A *Subject:* [High-Throughput-Technology-Community] High Throughput/High Content Technology Community Dear Colleague, Please could you cascade the following announcement to your respective departments. The meeting is next month so I appreciate your help with this. Best wishes Rob ..................................................................................................................................................................................................................................................................................................... *_FIRST ANNOUNCEMENT _** *High Throughput.jpg *INSTITUTE OF BIOMEDICAL ENGINEERING * *High Throughput/High Content Technology Community * *Inaugural Symposium * Wednesday 21st March 2012 12:00 – 18:00 Lecture Theatre G34, Sir Alexander Fleming Building, South Kensington Campus, Imperial College London _map <https://workspace.imperial.ac.uk/campusinfo/public/sthkencampus.pdf>_ *PLEASE PUT A NOTE IN YOUR DIARIES * In conjunction with the Francis Crick Institute, the Institute of Biomedical Engineering is pleased to announce the inaugural symposium of the High Throughput/High Content Technology Community. Please join us for a stimulating multidisciplinary programme of presentations. All welcome. This will be an excellent opportunity to meet and exchange ideas with colleagues from across the College. *Symposium * *Coffee and Registration at 12 noon * *Programme from 12:30 – 18:00 (see attached)* *Drinks Reception 18:00 * *To **register**for the symposium please visit: * *http://lwf-dev.cc.ic.ac.uk/bioeng/networks/event.php?event_id=6 * *(Please note that the above link only works on Imperial PCs. If you have problems registering, please use the registration forms on the Networks Membership page <http://www3.imperial.ac.uk/biomedeng/networks> ) or those attached.* *Dr Rob Fenton* *Consultant Research Advisor* *Institute of Biomedical Engineering* *RSM 4.23* *Royal School of Mines* *South Kensington Campus*** *Imperial College* *London SW7 2AZ* *Tel: 020 759 41500* *Mob: 07872 850122* *http://www3.imperial.ac.uk/people/r.fenton*

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[FILM-Users 00166] Fwd: [CISBIC-RA] [cisbio] FW: PDRA in image analysis
by Martin Spitaler 06 Feb '12

06 Feb '12

...forwarded for your information, Martin -------- Original Message -------- Subject: [CISBIC-RA] [cisbio] FW: PDRA in image analysis Date: Sun, 5 Feb 2012 14:39:20 +0000 From: Endres, Robert G <r.endres(a)imperial.ac.uk> To: cisbio <cisbio(a)imperial.ac.uk> FYI - open postdoc position in image analysis at Cambridge. Best, Robert ---------------------------------------------------------------- Dr. Robert Endres Senior Lecturer in Systems Biology Imperial College London Tel: +44 (0)20 7594 9537 r.endres(a)imperial.ac.uk Personal: http://www.imperial.ac.uk/people/r.endres Group: http://www3.imperial.ac.uk/biologicalphysics ________________________________________ From: Alfonso Martinez Arias [ama11(a)hermes.cam.ac.uk] Sent: 05 February 2012 10:13 To: Endres, Robert G Subject: PDRA in image analysis Dear Robert, With apologies for the trouble I wonder if you could post or pass this around. I am looking for a postdoc who is interested in image analysis and modelling and your department should be a good place to find such a person. With best wishes Alfonso Alfonso Martinez Arias Department of Genetics University of Cambridge Cambridge CB2 3EH UK Post-Doctoral Research Associate ------------------------------------------------------------------------ Applications are invited for a postdoctoral researcher to join an interdisciplinary research group and participate in a European Research Council (ERC) funded project on the dynamics of gene expression during stem cell differentiation. You should have experience in biological image analysis and knowledge of software tool development, with a focus on usability, and an interest in working with experimentalists. Knowledge of microscopy will be an advantage. http://www.jobs.cam.ac.uk/job/-12592/

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[FILM-Users 00165] Fwd: Places still available on RMS Light Microscopy Course
by Martin Spitaler 06 Feb '12

06 Feb '12

...forwarded for your information. By the way, this is a very good microscopy course (you can ask Mark for more details, he attended a few years ago). And just a reminder, there are plenty more interesting courses and meetings on the FILM website under LINKS & EVENTS (www.imperial.ac.uk/imagingfacility/links/events) Martin ################################################################ LISTSERV mailing list manager LISTSERV 15.0 Subject: Places still available on RMS Light Microscopy Course From: Victoria Masters <[log in to unmask]> Reply-To: Confocal Microscopy List <[log in to unmask]> Date: Fri, 3 Feb 2012 03:55:08 -0600 Content-Type: text/plain ***** RMS Light Microscopy Summer School 9-11 July 2012 University of York, UK Organiser: Dr Peter O'Toole There are still places left on the Royal Microscopical Society Light Microscopy Summer School. The Light Microscopy summer school is an annual, residential three day course held at the University of York covering the principles of light microscopy. Participants are also trained in practical issues surrounding light microscopy. After introductory presentations, the course is taught predominantly through hands-on practical sessions. Full details can be found at www.rms.org.uk/lmschool. If you have any questions, please contact Sarah Bebb, sarah(a)rms.org.uk.

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[FILM-Users 00164] Fwd: Fwd: February 2012 Events Newsletter
by Martin Spitaler 03 Feb '12

03 Feb '12

...forwarded for your information, Martin Begin forwarded message: > *From:* The Royal Society > <events.qsvysqeninbpziv(a)newsletters.royalsociety.org > <mailto:events.qsvysqeninbpziv@newsletters.royalsociety.org>> > *Date:* 1 February 2012 17:13:35 GMT > *To:* <t.magee(a)imperial.ac.uk <mailto:t.magee@imperial.ac.uk>> > *Subject:* *February 2012 Events Newsletter* > *Reply-To:* Royal Society Events <events(a)royalsociety.org > <mailto:events@royalsociety.org>> > > If you are having difficulties viewing this email, click here for an online version: <http://newsletters.royalsociety.org/q/14srFz4xb70g0b/wv> > > Forward to a Friend > The Royal Society > February 2012 Events Newsletter > > Dear Subscriber, > > Welcome to the Royal Society's monthly events newsletter which contains information on the Royal Society's public programme of evening lectures in the coming months as well as news on some of our other activities. > > 1. Royal Society events > 2. royalsociety.tv > 3. R.Science podcast > 4. Newsletter sign-ups > > 1. Royal Society events > > > > How new science is transforming the optical microscope > The 2012 Leeuwenhoek Lecture by Dr Brad Amos FRS > 13 February 2012, 6.30pm-7.30pm > The Royal Society, London > > There are two rules for making an optical microscope; the lenses must be small and they must capture light from the object over as wide an angle as possible. This was understood a century ago, however, lasers, computers and chemically-specific labels have recently produced an explosion of development. A new method will be described, which can show anatomy at the scale of micrometres and subcellular detail in the same image of a specimen such as a mouse embryo. This is the first microscope system to disobey the small lens rule. Brad Amos is an Emeritus Research Group Leader in the MRC Lab of Molecular Biology in Cambridge and a Visiting Scientist in the University of Strathclyde. > > Doors open at 5.45pm, seats allocated on a first-come-first-served basis. > > > © Copyright The Royal Society > The Royal Society > 6-9 Carlton House Terrace > London SW1Y 5AG > > t: 020 7451 2500 > e: events(a)royalsociety.org > w: royalsociety.org/events > > Registered Charity No 207043 > > The Royal Society : supporting excellence in science

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