December 2012 - FILM-Users

[FILM-Users 00299] competition and Christmas break
by Martin Spitaler 21 Dec '12

21 Dec '12

Dear microscopists, unfortunately despite a fair amount of pushing, the judges have not all come back to me with the scoring of the competition entries, and for unknown reasons the calendars have also been delayed, so we'll have to be patient with all this until the beginning of the new year. I'm really sorry for this, I've really tried hard to push everything through in time. The FILM Crew will be away during the college closure, and we'll be back refreshed from Wednesday 2 January 2013. I personally will be on a meeting directly afterwards, I'll be back at my desk a week later, from Wednesday 9 January, but Mark and Steve will be available earlier. Which leaves me to wish you all a relaxing holiday, a nice and peaceful Christmas break and a good start into the new year. Thank you all for the good cooperation in the facility, best wishes for your break from imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 0

[FILM-Users 00298] Fwd: bbsrc funded phd opportunity
by Martin Spitaler 10 Dec '12

10 Dec '12

...forwarded for your information; the original PDF can be found on our documentation server as usual, under <w:\film-user mailing list\postdoc-alistair-hume.pdf>; for those who don't know, Alistair used to work here in Miguel Seabra's group and then moved on to create his own research group in Nottingham. He was one of our best microscopists, and they also have a very good facility in Nottingham, so if you like microscopy, this should be very interesting, Martin -------- Original Message -------- Subject: bbsrc funded phd opportunity Date: Sun, 9 Dec 2012 20:36:18 +0000 From: Alistair Hume <Alistair.Hume(a)nottingham.ac.uk> Dear All, Apologies for the mass email but I'd appreciateit if you could pass the attached PhD opportunity on to any suitable students. With thanks and best wishes, Alistair Alistair Hume D.Phil. School of Biomedical Sciences University of Nottingham Medical School Queen's Medical Centre Nottingham, NG7 2UH UK Tel: +44(0)115823 0102 email: Alistair.hume(a)nottingham.ac.uk <mailto:Alistair.hume@nottingham.ac.uk> ################################################## BBSRC funded 4-year PhD Research Studentship available at the School of Biomedical Sciences University of Nottingham, UK. Title: Understanding the regulation of cancer associated protein Rab27. The small GTPase Rab27 is a general regulator of secretory granule transport and exocytosis. Its physiological importance is illustrated by the fact that Rab27 deficiency causes lethal immunodeficiency and partial albinism (Griscelli syndrome), while its over-expression drives the proliferation and metastasis of breast cancer and melanoma cells. However, in spite of the obvious patho-physiological importance of Rab27 function, little is known of the mechanism(s) regulating its activity. Thus the aim of this project is to determine the molecular mechanisms that regulate the activation and intracellular targeting of Rab27. This project will involve the use of a wide variety of cell and molecular biology techniques including cell culture, plasmid and siRNA transfection, fluorescence and confocal microscopy, live cell imaging and particle tracking, molecular cloning, RT-PCR, PCR, western blotting and protein-protein interaction studies, thereby providing comprehensive skills and training to students embarking on a career in biomedical research. This project will be jointly supervised by Alistair Hume and Peter Jones (for more details see: http://www.nottingham.ac.uk/biomedsci/) and will be within the newly established BBSRC doctoral training partnership hosted by the University of Nottingham and the Rothamstead Institute (for more details of the programme see: http://www.findaphd.com/search/PhDDetails.aspx?CAID=1032&LID=338 Applicants should have, or will expect to attain at least a 2i degree classification (or equivalent) in biochemistry, genetics, cell biology (or a related discipline). This studentship is available for a period of 4 years and provides a stipend of £13,590 per annum. Please note that certain eligibility conditions apply, and therefore, this studentship is only available to UK/EU citizens ordinarily resident in the UK. Successful applicants are identified in a 2-stage process involving appraisal by both the academic supervisors, and by the Doctoral Training Partnership Selection Panel. Contact: Alistair.hume(a)nottingham.ac.uk Start date October 2013. Deadline for application: Noon 9th January 2013

1 0

[FILM-Users 00297] FILM Calendar 2012
by Martin Spitaler 10 Dec '12

10 Dec '12

Dear microscopists, as in previous years, we will create a FILM Calendar from the top entries of our competition. Those of you who have submitted images will get one of those for free, but everyone else can also get one on request. You have to pay for it obviously, but for the quality you get, £21 is an adequate price. And I've just noticed Photobox (where I order them) has a special discount on, so if you are quick and order it from me today, you get them for ~£16. Please let me know; good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 0

[FILM-Users 00296] COMMERCIAL Fwd: mEOS2
by Martin Spitaler 07 Dec '12

07 Dec '12

COMMERCIAL...forwarded for your information, Martin -------- Original Message -------- Subject: mEOS2 Date: Fri, 7 Dec 2012 10:20:38 +0000 From: Dr Gavin Allsop <g.allsop(a)badrilla.com> To: <m.spitaler(a)IMPERIAL.AC.UK> Dear Martin I am writing to you from Badrilla Ltd (Leeds) to let you know that we have just launched an antibody to mEOS2, the photoactivatable / photoconvertible fluorescent protein that has recently emerged as a tool in super resolution microscopy. This antibody is the first of its kind, working in both WB and IF applications, I hope it will help researchers with the cloning and expression level assessment of their mEOS2 fusion proteins. To see validation data in relevant applications, please follow the link or get in touch to find out more. http://badrilla.com/meos2.html I look forward to hearing from you With best regards Gavin -------------- Dr Gavin Allsop Badrilla Ltd Leeds Innovation Centre 103 Clarendon Road Leeds LS2 9DF United Kingdom -------------- new http://badrilla.com http://email07.europe.secureserver.net/images/social_media/icon_sm_twitter.… <http://twitter.com/@BadrillaLtd>http://email07.europe.secureserver.net/images/social_media/icon_sm_facebook… <http://facebook.com/BadrillaLtd>

1 0

[FILM-Users 00283] FILM Image Of The Year 2012 competition
by Martin Spitaler 05 Dec '12

05 Dec '12

Dear microscopists, it is back, bigger and better than ever - the FILM IMAGE OF THE YEAR 2012 competition! Again, great prizes are waiting to be won, and this time everybody is a winner: Winner - an iPod Nano! ...for those long hours at the microscope Runners-up: a beautiful A3 poster calendar, worth £18! ALL PARTICIPANTS: 30% discount on Molecular Probes antibodies! Deadline for submission of your images: Wednesday 5th of December 2012 (see also the website: http://www3.imperial.ac.uk/imagingfacility/research/gallery/film_image_of_t…) Judges As in previous years, we have a prestigious panel of independent judges to select your best images: Kathy Nobel, Tate Modern Alex Ball, Natural History Museum Tatiana Schofield, Imperial College Consultants Melissa Belli, artist Sponsorship As before, we thank Molecular Probes / Life Technologies for their generous sponsorship! Rules for participation Up to 3 images per participant, but they must show different samples (not different versions of the same image) Images must have been taken on FILM microscopes (original data files must be made available) Images will be judged purely on their visual impression, they do not need to show any scientific results The best possible visualisation of the image data is encouraged (e.g. contrast enhancement, look-up tables), but manipulation of pixel data is not allowed - the scientific content is still visible; example for , manipulations that are not allowed are adding / removing of objects (retouching) or collages (copying objects together) By submitting the images, participants grant FILM the right to make the entries publicly available on the FILM website, use them for the calendars or other presentations; please make sure they don't contain any identifiable unpublished information So don't miss out, go through your microscopy images and go for the prizes! Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 2

[FILM-Users 00294] Fwd: RE: tet-regulated GFP
by Martin Spitaler 04 Dec '12

04 Dec '12

...forwarded, a request by a former FILM user; if you can help, please get in contact with Miranda directly ("Wilson, Miranda" <miranda.wilson(a)ucl.ac.uk>), Martin On 03/12/2012 14:06, Wilson, Miranda wrote: > Dear Martin > > I did an MRes project a few years ago with Alistair in the Seabra lab, > tracking melanosomes. You were really helpful back then and I wonder > if you could help me out again? These days I'm a postdoc at the MRC > institute at UCL. I'm hoping to get hold of a*tet-regulated GFP or GFP > fusion plasmid that I can use to help me screen the HCT116-tetR cell > line* I'm making. Do you know if anyone at SAF has one of those? > > Thanks and best wishes > Miranda > > > > Miranda S C Wilson, PhD > Postdoctoral Researcher > Saiardi Lab > MRC Laboratory for Molecular Cell Biology > University College London > Gower Street, London, WC1E 6BT > +44 207 679 2657 <tel:207%20679%202657> >

1 0