November 2012 - FILM-Users

[FILM-Users 00282] Annual FILM report 2011-12
by Martin Spitaler 15 Nov '12

15 Nov '12

Dear FILM Users, Please find the annual FILM report 2011-12 on the FILM website under RESOURCES / DOCUMENTS (https://workspace.imperial.ac.uk/imagingfacility/Internal/FILM%20report%202…) Overall it’s been another good year for FILM in difficult financial circumstances. The establishment of the L block satellite has been one highlight. Please let me or Martin know if you have any comments or queries. Many thanks. And at this place a reminder: You may not be aware of the financial model FILM operates on – College requires us to recover /all/ our costs but /not/ to make a surplus. You can imagine this is not a straightforward challenge, but we do our best and so far have thankfully broken even or made only a slight surplus! To set our charges for the upcoming year, in essence what we do is divide our costs (which are more or less fixed – service contracts, consumables/minor equipment and salaries) by the number of hours usage of the microscopes and other services we offer. So you can see that this simple calculation means that the more the microscopes get used, the less we can charge for usage in the next year – so, the more you use FILM, the cheaper it gets! Best, Tony Anthony I. Magee Professor of Membrane Biology Head of Section of Molecular Medicine National Heart and Lung Institute Faculty of Medicine Imperial College London Sir Alexander Fleming Building Exhibition Road South Kensington London SW7 2AZ UK Tel. +44 (0)20 7594 3135 FAX +44 (0)20 7594 3015 http://www1.imperial.ac.uk/nhli/molecular/lipidation/ http://www1.imperial.ac.uk/medicine/people/t.magee/

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[FILM-Users 00281] Fwd: CONFOCALMICROSCOPY Index - 8 Nov 2012 to 9 Nov 2012 (#2012-267)
by Martin Spitaler 15 Nov '12

15 Nov '12

...forwarded for your information: This new ImageJ plugin could save us all a lot of time! Martin -------- Original Message -------- Subject: CONFOCALMICROSCOPY Index - 8 Nov 2012 to 9 Nov 2012 (#2012-267) Date: Sat, 10 Nov 2012 00:05:42 -0600 From: CONFOCALMICROSCOPY automatic digest system <LISTSERV(a)LISTS.UMN.EDU> Reply-To: <CONFOCALMICROSCOPY-Search-request(a)LISTS.UMN.EDU> To: <CONFOCALMICROSCOPY(a)LISTS.UMN.EDU> *Subject:* New ImageJ plugin for figure creation. *From:* Jerome Mutterer <jerome.mutterer(a)IBMP-ULP.U-STRASBG.FR> *Reply-To:* Confocal Microscopy List <CONFOCALMICROSCOPY(a)LISTS.UMN.EDU> *Date:* Fri, 9 Nov 2012 15:45:42 +0100 *Content-Type:* text/plain ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear all, Today, we are releasing the first public beta version of FigureJ, an ImageJ plugin that will help you build articles figures from your images. It reduces the time needed to build a figure from hours to minutes, works with the standard ImageJ release, and adds some very interesting features like it keeps track of original datasource and processing. Adding scalebars for example is made very easy. Figure annotation can be done with ImageJ built-in tools. Check out the videos on the ImageJ documentation wiki to see how simple it is (http://goo.gl/yIzq3) <http://goo.gl/yIzq3%29.> This is the first public version, so it certainly has bugs in it, and the code is not finished. Of course it's free and open-source, so everyone is welcome to suggest or contribute enhancements. We acknowledge the support of the Institute for Plant Molecular Biology, CNRS , Strasbourg, France; HTW Berlin, Germany, and Carl Zeiss. All comments are welcome. Sincerely, Jerome. http://imagejdocu.tudor.lu/doku.php?id=plugin:utilities:figurej:start CONFOCALMICROSCOPY Index - 8 Nov 2012 to 9 Nov 2012 (#2012-267) Browse the CONFOCALMICROSCOPY online archives. <http://lists.umn.edu/cgi-bin/wa?LIST=CONFOCALMICROSCOPY> Powered by the LISTSERV Email List Manager <http://www.lsoft.com/products/listserv-powered.asp>

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[FILM-Users 00280] Fwd: [neuroscience-technology-network] Carandini Lecture Mon 5pm, UCL
by Martin Spitaler 08 Nov '12

08 Nov '12

...forwarded for your information. The original flyer is on the FILM documentation server at <\\film-nas.med.ic.ac.uk\documentation\film-user mailing list\121112 Matteo Carandini Lecture.pdf> Sainsbury Wellcome Centre Lecture Series on Neural Circuits & Behaviour Professor Matteo Carandini "Probing spatial computations in mouse visual cortex" MONDAY 12th NOVEMBER 5pm AV HILL LECTURE THEATRE HOST: John O'Keefe Please join us for a glass of wine afterwards Starling Room- Medical Sciences Room 250 -------- Original Message -------- Subject: [neuroscience-technology-network] Carandini Lecture Mon 5pm, UCL Date: Thu, 8 Nov 2012 13:15:44 +0000 From: Simon Schultz <s.schultz(a)imperial.ac.uk> Reply-To: <s.schultz(a)imperial.ac.uk> To: neuroscience-technology-network <neuroscience-technology-network(a)imperial.ac.uk> Dear all, Please see attached as a PDF details of a lecture by Matteo Carandini at UCL on monday. Cheers, Simon

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[FILM-Users 00279] Fwd: Re: Super-resolution symposia Thursday May 3rd LMCB 5pm
by Martin Spitaler 08 Nov '12

08 Nov '12

...forwarded for your information. The attachment is at <\\film-nas.med.ic.ac.uk\film-user mailing list\121206 Super-resolution Club.pptx> Martin -------- Original Message -------- Subject: Re: Super-resolution symposia Thursday May 3rd LMCB 5pm Date: Thu, 1 Nov 2012 13:54:35 +0000 From: Ann Wheeler <a.p.wheeler(a)qmul.ac.uk> To: Parsons, Maddy <maddy.parsons(a)kcl.ac.uk> CC: Nick Barry <nbarry(a)mrc-lmb.cam.ac.uk>, Daniel Metcalf <daniel.metcalf(a)npl.co.uk>, Ian Dobbie <ian.dobbie(a)bioch.ox.ac.uk>, "neveen hosny" <n.hosny(a)imperial.ac.uk>, Andrew Vaughan <andrew.vaughan(a)UCL.AC.UK>, "Cox, Susan" <susan.cox(a)kcl.ac.uk>, "Harris, John" <john.l.harris(a)kcl.ac.uk>, "French, Paul (PHOT) M W (Photonics)" <paul.french(a)imperial.ac.uk>, "Martin Knight" <m.m.knight(a)qmul.ac.uk>, Andrevi Sapelkin <a.sapelkin(a)qmul.ac.uk>, John Connelly <j.connelly(a)qmul.ac.uk>, <wen.wang(a)qmul.ac.uk>, <sunil.kumar(a)imperial.ac.uk>, <rainer.heintzmann(a)kcl.ac.uk>, <simon.ameer-beg(a)kcl.ac.uk>, <cartera(a)mrc-lmb.cam.ac.uk>, <anne.vaahtokari(a)cancer.org.uk>, Angray Kang <a.s.kang(a)qmul.ac.uk>, <alex.knight(a)npl.co.uk>, <ejr36(a)cam.ac.uk>, <meerdelyi(a)gmail.com>, "Jones, Gareth" <gareth.jones(a)kcl.ac.uk>, Martin Spitaler <m.spitaler(a)imperial.ac.uk>, Yan Gu <ygu(a)nimr.mrc.ac.uk>, Ricardo Henriques <paxcalpt(a)gmail.com>, Microscopy List <UK-MICROSCOPY(a)JISCMAIL.AC.UK>, "Wright, Colin" <colin.wright(a)nikon.co.uk>, "Khaliq, Ghazanfar" <ghazanfar.khaliq(a)nikon.co.uk> Dear All This is to let you know that the second meeting of the super-resolution club has been arranged for Thursday December 6th at the LMCB at UCL at 5pm. Dr Ricardo Henriques, Institute Pasteur, France. will give a talk titled: 'A molecular scale view into cells through super-resolution microscopy' Please do pass this message onto interested colleagues. Best wishes Ann Dr Ann Wheeler BALM Facility Manager Blizard Institute 4 Newark Street London E1 2AT Tel: 020 7882 2406

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[FILM-Users 00278] Fwd: FW: interesting seminar Monday November 12 - Alan Waggoner - Fluorescent biosensors and targeted physiological indicators for cell pathways
by Martin Spitaler 06 Nov '12

06 Nov '12

...forwarded for your information - should be a good seminar. As usual, the attachment (PDF) is on the FILM Documentation server under <\\film-nas.med.ic.ac.uk\documentation\film-user mailing list\121112 LSF Poster Waggoner London screen.pdf>, Martin -------- Original Message -------- Subject: FW: interesting seminar Monday November 12 - Alan Waggoner - Fluorescent biosensors and targeted physiological indicators for cell pathways Date: Mon, 5 Nov 2012 18:25:40 +0000 From: French, Paul (PHOT) M W (Photonics) <paul.french(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, Please could you email this invitation to an exciting imaging seminar -- with drinks reception - to the FILM mailing list and any other colleagues that you think might be interested. Alan Waggoner is one of the pioneers of fluorescence-based sensors and is currently working on new strategies for live cell imaging of cell pathways with small molecule dyes. All are welcome. Please can you forward the message topotentially interested colleagues. Best wishes, Paul November 12-14: "Fluorescent biosensors and targeted physiological indicators for cell pathways" Keynote Speaker Prof. Alan S. Waggoner Director of Molecular Biosensor and Imaging Center Department of Biological Sciences, Carnegie Mellon University, Pittsburgh / US Venues * *LSF London - Imperial College *Monday, November 12 -- 5:30pm. Chair: Prof. Paul French Venue: Imperial College London, Lecture Theatre 3, Blackett Laboratory, Prince Consort Road, London SW7 2BB Lecture Alan S. Waggoner: We develop fluorescent biosensors toolkits for pathways of living cells, focussing on smart biosensors for high throughput methods. Our biosensor platform consists of Fluorescence Activating Proteins (FAPs) that bind fluorogen dyes with large fluorescence increases. Sensors have been developed to rapidly and selectively track FAP labeled proteins such as GPCRs. This platform is proving promising for developing calcium, pH, sodium, potassium and redox sensors that will emit signals only from investigator-targeted regions of a cell. Sensors for protein-protein interactions and protein modification are in progress. Learn more about Alan S. Waggoner: <http://www.cmu.edu/bio/faculty/waggoner.html> Invitation The members of the Scientific Advisory Board UK - Prof. Paul French (Imperial College London), Prof. Antony Galione (Oxford Univ.), Dr. Jim Haseloff (Cambridge Univ.), Prof. David Klenerman (Cambridge Univ.), Prof. Gero Miesenboeck (Oxford Univ.), Prof. Tony Ng (King's College London), Dr. Violaine Sée, Dr. Bob Burgoyne, Prof. Alexei Tepikin (Liverpool Univ.), Prof. Mike White (Manchester Univ.), Prof. Keith Willison (Institute of Cancer Research London), Prof. Tony Wilson (Oxford Univ.), and Dr. Thomas Zapf (Leica Microsystems) - cordially invite you to meet Alan Waggoner at the post lecture receptions for personal discussion. Registration All lectures are free of charge. For organizational reasons please RSVP by e-mail to LSF(a)leica-microsystems.com <mailto:LSF@leica-microsystems.com>. For more information see <http://www.leica-microsystems.com/events/leica-scientific-forum/lsf-details…>

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[FILM-Users 00277] Fwd: FW: interesting seminar Monday November 12 - Alan Waggoner - Fluorescent biosensors and targeted physiological indicators for cell pathways
by Martin Spitaler 06 Nov '12

06 Nov '12

...forwarded for your information - should be a good seminar. As usual, the attachment (PDF) is on the FILM Documentation server under <\\film-nas.med.ic.ac.uk\documentation\film-user mailing list\121112 LSF Poster Waggoner London screen.pdf>, Martin -------- Original Message -------- Subject: FW: interesting seminar Monday November 12 - Alan Waggoner - Fluorescent biosensors and targeted physiological indicators for cell pathways Date: Mon, 5 Nov 2012 18:25:40 +0000 From: French, Paul (PHOT) M W (Photonics) <paul.french(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin, Please could you email this invitation to an exciting imaging seminar -- with drinks reception - to the FILM mailing list and any other colleagues that you think might be interested. Alan Waggoner is one of the pioneers of fluorescence-based sensors and is currently working on new strategies for live cell imaging of cell pathways with small molecule dyes. All are welcome. Please can you forward the message topotentially interested colleagues. Best wishes, Paul November 12-14: "Fluorescent biosensors and targeted physiological indicators for cell pathways" Keynote Speaker Prof. Alan S. Waggoner Director of Molecular Biosensor and Imaging Center Department of Biological Sciences, Carnegie Mellon University, Pittsburgh / US Venues * *LSF London - Imperial College *Monday, November 12 -- 5:30pm. Chair: Prof. Paul French Venue: Imperial College London, Lecture Theatre 3, Blackett Laboratory, Prince Consort Road, London SW7 2BB * *LSF Oxford - University of Oxford *Tuesday, November 13 -- 4:00pm. Chair: Prof. Gero Miesenboeck Venue: Main Seminar Room, Biochemistry Department, South Parks Road, Oxford * *LSF Liverpool - Liverpool University *Wednesday, November 14 -- 5:00pm. Chair: Prof. Violaine Sée Venue: Lecture Theatre 3, Life Sciences Building, Crown Street, Liverpool L69 7ZB Lecture Alan S. Waggoner: We develop fluorescent biosensors toolkits for pathways of living cells, focussing on smart biosensors for high throughput methods. Our biosensor platform consists of Fluorescence Activating Proteins (FAPs) that bind fluorogen dyes with large fluorescence increases. Sensors have been developed to rapidly and selectively track FAP labeled proteins such as GPCRs. This platform is proving promising for developing calcium, pH, sodium, potassium and redox sensors that will emit signals only from investigator-targeted regions of a cell. Sensors for protein-protein interactions and protein modification are in progress. Learn more about Alan S. Waggoner: <http://www.cmu.edu/bio/faculty/waggoner.html> Invitation The members of the Scientific Advisory Board UK - Prof. Paul French (Imperial College London), Prof. Antony Galione (Oxford Univ.), Dr. Jim Haseloff (Cambridge Univ.), Prof. David Klenerman (Cambridge Univ.), Prof. Gero Miesenboeck (Oxford Univ.), Prof. Tony Ng (King's College London), Dr. Violaine Sée, Dr. Bob Burgoyne, Prof. Alexei Tepikin (Liverpool Univ.), Prof. Mike White (Manchester Univ.), Prof. Keith Willison (Institute of Cancer Research London), Prof. Tony Wilson (Oxford Univ.), and Dr. Thomas Zapf (Leica Microsystems) - cordially invite you to meet Alan Waggoner at the post lecture receptions for personal discussion. Registration All lectures are free of charge. For organizational reasons please RSVP by e-mail to LSF(a)leica-microsystems.com <mailto:LSF@leica-microsystems.com>. For more information see <http://www.leica-microsystems.com/events/leica-scientific-forum/lsf-details…>

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