January 2012 - FILM-Users - imperial.ac.uk

[FILM-Users 00162] FILM software: Volocity and Definiens
by Martin Spitaler 31 Jan '12

31 Jan '12

Dear microscopists, because I've received several requests recently, here some update on our software servers: We have a licence server for the VOLOCITY software, which means you can download, install and run the fully licences version on your own computer. All you need is to ask us for a login and type in the server details when starting the software. Volocity also imports images from Zeiss and Leica confocals and is very powerful for 3D rendering, visualisation, tracking and some 3D quantification. For an overview, see the Volocity website <http://www.perkinelmer.com/pages/020/cellularimaging/products/volocity.xhtml> For more demanding or medium / high throughput image analysis, we have the very powerful DEFINIENS software. This also uses a licence server, so again you can download, install and run it on your own computer. It's very powerful but also a bit complex, if you are interested please get in contact, so we can give you an introduction, training material and help. Because the software licences cost money (>£4.5k per year for Volocity, £2.5 for Definiens), we need to charge for usage. Luckily we have many users, so currently we only charge £3 per hour. Please let us know if you want to use either software package. Certainly good visualisation and quantification can make a big difference on what you get out of your data, and the hard work you've put in to get them. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 0

[FILM-Users 00161] TODAY: FILM Club: Kieran McGourty - Inhibition of lysosome function by Salmonella
by Martin Spitaler 27 Jan '12

27 Jan '12

...just a reminder about our FILM Club today, Martin ===================================================================== Dear microscopists, already the month is nearing its end, so it's time for the next FILM Club. Unfortunately we had to postpone it last time (sorry for those who turned up anyway), but this week it's really happening: Friday 27 Jan 2012, 13.00, SAFB-120 (South Kensington) KIERAN McGOURTY: INHIBITION OF LYSOSOME FUNCTION IN SALMONELLA For this study, Kieran measured the pH inside the cytosol and lysosomes with pH-sensitive dyes. He went to great lengths to measure the pH in a reproducible and quantifiable way. In this FILM Club he will explain how he has managed to calibrate the fluorescence signal, which dyes turned out best for measurement of the acidic lysosomes, and obviously what interesting results he achieved with all this. See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 0

[FILM-Users 00160] FILM Club this Friday: Kieran McGourty - Inhibition of lysosome function by Salmonella
by Martin Spitaler 24 Jan '12

24 Jan '12

Dear microscopists, already the month is nearing its end, so it's time for the next FILM Club. Unfortunately we had to postpone it last time (sorry for those who turned up anyway), but this week it's really happening: Friday 27 Jan 2012, 13.00, SAFB-120 (South Kensington) KIERAN McGOURTY: INHIBITION OF LYSOSOME FUNCTION IN SALMONELLA For this study, Kieran measured the pH inside the cytosol and lysosomes with pH-sensitive dyes. He went to great lengths to measure the pH in a reproducible and quantifiable way. In this FILM Club he will explain how he has managed to calibrate the fluorescence signal, which dyes turned out best for measurement of the acidic lysosomes, and obviously what interesting results he achieved with all this. See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 0

[FILM-Users 00158] server problem
by Martin Spitaler 20 Jan '12

20 Jan '12

Dear microscopists, we are having a problem with our facility server, first it was very slow, now it's completely inaccessible. I hope ICT will help us getting it up very soon, I'll let you know. Sorry for the troubles, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 1

[FILM-Users 00157] Non glycerol based mountigng media
by Bo, Chiara 17 Jan '12

17 Jan '12

Dear all, has anybody got a non-glycerol based mounting media that I could try tomorrow? I had some problems with lipid dies that were removed by the glycerol based one. Thanks, Chiara Bo

1 0

[FILM-Users 00156] Fwd: TODAY: Molecular Medicine & Cell Biology Seminar Series | room 121, SAF Building
by Martin Spitaler 17 Jan '12

17 Jan '12

...forwarded, for your information, Martin -------- Original Message -------- Subject: REMINDER: Molecular Medicine & Cell Biology Seminar Series | TODAY | room 121, SAF Building Date: Tue, 17 Jan 2012 10:10:49 +0000 From: Moore, Peter J <peter.moore(a)imperial.ac.uk> Molecular Medicine & Cell Biology Seminar Series: Prof Dan Davis, Cell & Molecular Biology, FoNS, Imperial College: "USING SUPER-RESOLUTION MICROSCOPY TO WATCH IMMUNE CELLS KILL" Host: Tony Magee Tuesday 17 January, 12.00-13.00, SAFB 121, South Kensington Campus Peter Moore Section Administrator Molecular Medicine Section & Cardiovascular Sciences at the Royal Brompton Campus NHLI, Imperial College 360 (Q), Level 3, Sir Alexander Fleming Building London SW7 2AZ tel: 020 759 43175 email: peter.moore(a)imperial.ac.uk <mailto:peter.moore@imperial.ac.uk>

1 0

[FILM-Users 00154] Seminar Dylan Owen, FRIDAY 06 January | 12:00-1:00pm | Room 120 SAF
by Martin Spitaler 06 Jan '12

06 Jan '12

Dear microscopists, we are starting the new year with a interesting seminar by Dylan Owen, who used to be a PhD student in Tony Magee's and Paul French's lab, and now works as a postdoc in the lab of Katharina Gaus in Sydney: Molecular Medicine & Cell Biology Seminar Series Dr Dylan Owen University of New South Wales (Sydney, Australia) ADVANCED FLUORESCENCE MICROSCOPY OF MEMBRANE LIPID AND PROTEIN DYNAMICS AND DISTRIBUTIONS Host: A Magee Friday 6 January 12.00-13.00 SAFB 120, South Kensington See you there, happy new year and good imaging, Martin -------- Original Message -------- Subject: Molecular Medicine & Cell Biology Seminar Series | FRIDAY 06 January | 12:00-1:00pm | Room 120 SAF Date: Wed, 21 Dec 2011 10:22:27 +0000 From: Moore, Peter J <peter.moore(a)imperial.ac.uk> Peter Moore Section Administrator Molecular Medicine Section & Cardiovascular Sciences at the Royal Brompton Campus NHLI, Imperial College 360 (Q), Level 3, Sir Alexander Fleming Building London SW7 2AZ tel: 020 759 43175 email: peter.moore(a)imperial.ac.uk <mailto:peter.moore@imperial.ac.uk> -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

1 1