August 2011 - FILM-Users - imperial.ac.uk

[FILM-Users 00092] Definiens training dates
by Martin Spitaler 13 Sep '11

13 Sep '11

Dear microscopists, it's been tricky to find a common date between room and all people involved, and it's now later than originally planned, but now the Definiens Basic training is now fixed: Training course: Definiens Basic I Time & date: Tue 27 - Thu 29 September 2011, 10.00-17.00 Location: Computer Training Room in the Central Library, 2nd floor (South Kensington Campus) Trainer: Thomas Mrowiec, Definiens Available places is 6-10 Costs: max £500 (based on 6 participants, cheaper with more) Definiens Developer XD: We have had this software in the facility for just over a year now, and it's already proved its value for some very challenging image analysis projects which would have been impossible for us otherwise (the most demanding of them the fully automated quantification of immunological synapse formation in 30,000 time frames). The software offers easy access to a huge range of image analysis tools, and is specialised in analysing images on multiple scales, taking the biological context into account. I don't have any shares in the company, but it's just extremely useful, and with a good introduction - like this training course - pretty straightforward to use (recently a user with no experience in image analysis commented 'this is much easier than ImageJ'). I have also noticed that this training course is an excellent entry into the complex field of image analysis, whatever software tool you are using in the future. Trainer and training: Thomas Mrowiec comes from the Definiens Academy, specialising in customer training. The training comes with very detailed training material and sample datasets, and it doesn't require any previous experience. However, users are welcome to bring along their own sample images and get ideas how best to start with their analysis. Arranging the training on-site here we are able to keep the costs to a minimum (no travelling or accomomdation costs, larger number of participants). If you are interested, please book and confirm your place as soon as possible. Good imaging, Martin ================================= Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00099] Fwd: FW: Cell Press Webinar on Super-resolution
by Martin Spitaler 23 Aug '11

23 Aug '11

...forwarded for your information, Martin *From:*Cell Press [mailto:cellpress@mail.cell-press.com] *Sent:* 10 August 2011 22:48 *To:* Magee, Tony *Subject:* Cell Press Webinar on Super-resolution Cell Press <http://mail.cell-press.com/go.asp?/bECE001/mYS68F2F/qP1XQF2F/uAKZX32F/xR4PI…> Register for a Free Webinar ------------------------------------- Super-resolution: light microscopy for the 21st century ------------------------------------- Live date: *Thursday, September 8, 2011* 11:00 AM EST -------------------------------------Register Today, It's Free... <http://mail.cell-press.com/go.asp?/bECE001/mYS68F2F/qG16ZF2F/uAKZX32F/xR4PI…> During the webinar, you will: - Hear from our panel of experts about the latest approaches in super-resolution microscopy. - Learn how super-resolution microscopy is being applied to drive cell biology to a new level. In 1873, Ernst Abbe developed a theory that defined the limit of resolution of the light microscope. Following suit from astronomy, Abbe defined resolution as the ability to resolve, as separate, two point sources of light. The Abbe limit of 200 - 300 nm is based upon the ability of the light microscope to collect only a subset of spatial frequencies and the physiological properties of the human eye. Starting in the 1980's with the advent of video, and now digital microscopy, microscopists began to push these limits, based upon the linear response of video detectors. True super-resolution techniques, such as scanning nearfield microscopy, which collects the higher spatial frequencies resident in the evanescent wave were developed. While pushing the resolution limit an order of magnitude to ~ 20 nm these techniques had limited applicability to the demands of modern cell biology. Starting in the 1990's, a whole new spectrum of super-resolution techniques have ushered in a renaissance in biological microscopy. These techniques employ single molecule detection approaches and the non-linear absorption properties of fluorescent materials to restructure the microscope's point spread function. In this webinar, leaders in the field of super-resolution will explain the physical bases of these new approaches and describe how they will redefine our understanding of cell and molecular biology in the 21st century. Register Today, It's Free... <http://mail.cell-press.com/go.asp?/bECE001/mYS68F2F/q71FZF2F/uAKZX32F/xR4PI…> *Unable to join us on September 8?*This webinar will be archived for 6 months for on-demand viewing. Register today to ensure you receive updates on how to gain access after the live event! <http://mail.cell-press.com/go.asp?/bECE001/mYS68F2F/qYSFZF2F/uAKZX32F/xR4PI…> Speakers: *Dr. Stefan Hell*, Director, Department of NanoBiophotonics; Director Analytical and Quantitative Light Microscopy Course *Dr. Hari Schroff*, Chief and Investigator, Section on High Resolution Optical Imaging; National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health *Dr. Paul R. Selvin*, Professor of Physics University of Illinois at Urbana-Champaign Moderated by: *Dr. David E. Wolf*, Group Leader Optics and Photonics Radiation Monitoring Devices; Director Analytical and Quantitative Light Microscopy Course Brought to you by: Biophysical Journal <http://mail.cell-press.com/go.asp?/bECE001/mYS68F2F/qPSOZF2F/uAKZX32F/xR4PI…> Cell Press To unsubscribe, please click here <http://mail.cell-press.com/go.asp?/bECE001/mYS68F2F/qP168F2F/uAKZX32F/xR4PI…>

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[FILM-Users 00098] FILM staff holiday update
by Martin Spitaler 23 Aug '11

23 Aug '11

Dear microscopists, just an update on our holiday plans. Mark is away this week, and I will be away for two weeks from tomorrow. Luckily Steve is back in action now, so for anything urgent please get in contact with him. Good imaging, Martin ================================== Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00096] Fwd: interesting job opportunity at HIH
by Martin Spitaler 15 Aug '11

15 Aug '11

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[FILM-Users 00095] OMERO Workshop
by Martin Spitaler 12 Aug '11

12 Aug '11

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