March 2011 - FILM-Users - imperial.ac.uk

[FILM-Users 00052] FILM Image Of The Year - the winner
by Martin Spitaler 22 Mar '11

22 Mar '11

Dear microscopists, the winner of the FILM Image Of The Year 2011 is decided: It's Valentina Caorsi with her detail of a mosquito wing. Congratulations, you have won a Canon Powershot A495, sponsored again by Invitrogen. For her winning image and all other impressive entriies, have a look at the FILM website / RESEARCH & GALLERY / GALLERY / FILM IMAGE OF THE YEAR (unfortunately this mailing list doesn't allow html mails) All entrants will again get a beautiful "spring calendar" (April 2011-March 2012) with the top images (please let me know if you are interested, it has happened that people haven't picked theirs up). If anyone else wants to order a calendar please let me know, they cost ~£19-20 (depending on the numbers ordered). Thanks for all participants for the again very impressive entries, good imaging, Martin Link to the images: http://www3.imperial.ac.uk/imagingfacility/research/gallery/film_image_of_t… ############################################### Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00051] Fwd: Live/dead E.coli staining
by Martin Spitaler 22 Mar '11

22 Mar '11

...can anyone help Parul? Typical nuclear dyes (e.g. PE) don't seem to go into bacteria easily, even when they are dead, Martin -------- Original Message -------- Subject: Live/dead E.coli staining Date: Tue, 22 Mar 2011 16:46:43 +0000 From: Mehta, Parul <p.mehta10(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk>, film-users <film-users(a)imperial.ac.uk> Hi, I wanted to know if anyone of the FILM users have done live/dead E.coli staining to differentiate between live E.coli cells to dead cells. Many thanks and kind regards, Parul Mehta PhD student Martin Buck's lab, 4th floor SAF Division of Biology Faculty of Life Sciences Imperial College London Sir Alexander Fleming Building South Kensington Campus London SW7 2AZ

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[FILM-Users 00050] FILM Club: topic needed
by Martin Spitaler 21 Mar '11

21 Mar '11

Dear microscopists, unfortunately due to a looming deadline the planning for this month's FILM Club didn't work out, so we need a volunteer. Sorry for the short notice, it's already going to be this Friday (though if that's a problem, we could postpone it by a week), but who would like to present some interesting technique? Don't be shy, please come forward, we had recently a few FILM Clubs with more exotic presentations, but don't forget that the primary aim is to simply exchange experience, methods, tools in a very informal way, so don't think your own project is not interesting enough. And you might end up with a new collaboration, it has already happened in previous FILM Clubs. Alternatively, if you are specifically interested in a method, let me know as well, and I'll try to find someone to talk about it. So please get back, good imaging, Martin ############################## Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00049] Fwd: Meeting on Foerster Energy Transfer, Göttingen, Mach 27-30
by Martin Spitaler 18 Mar '11

18 Mar '11

...for your information; very short notice, sorry, but I only just got it in, Martin -------- Original Message -------- Subject: Meeting on Foerster Energy Transfer, Göttingen, Mach 27-30 Date: Fri, 18 Mar 2011 14:39:16 +0000 From: Wolfgang Becker <becker(a)becker-hickl.de> To: Claus Seidel <cseidel(a)hhu.de> *Dear Microscopy / FLIM User, * please join the *International Meeting* *on Foerster Resonance Energy Transfer in Life Sciences Göttingen March 27-30, 2011* *Organised by * *Max Planck Institute for Biophysical Chemistry* *Göttingen* For deatils and registration please see http://fret.uni-duesseldorf.de/ Best regards Wolfgang Becker Becker & Hickl GmbH Berlin, Germany www.becker-hickl.com becker(a)becker-hickl.com Tel. +49 30 787 5632 Prof. Dr. Claus Seidel Heinrich-Heine-Universitaet Duesseldorf Lehrstuhl fuer molekulare physikalische Chemie Universitaetsstr.1 Geb. 26.32.02 40225 Duesseldorf, Germany Tel:++49/211/81-15880 (Sek) - 15881(Office) Fax:++49/211/81-12803

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[FILM-Users 00048] Fwd: Membrane bound FP mouse line
by Martin Spitaler 16 Mar '11

16 Mar '11

Dear microscopists, can anyone help? If so, please contact Bertrand directly, <b.vernay(a)ich.ucl.ac.uk>. Thanks, and good imaging, Martin -------- Original Message -------- Subject: Membrane bound FP mouse line Date: Wed, 16 Mar 2011 16:42:40 +0000 From: Bertrand Vernay <b.vernay(a)ich.ucl.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Dear Martin Following a request from a user at the ICH confocal facility, I'm trying to find a mouse transgenic line expressing a membrane bound fluorescent protein. Are you aware of any of your users having such a mouse line at FILM or elsewhere at Imperial? Thank you for your help Bertrand Dr Bertrand Vernay Microscopy Senior Research Associate Developmental Biology Unit UCL Institute of Child Health 30 Guilford Street London WC1N 1EH, UK Tel : (+44) 020 7905 2224 (direct line) (+44) 020 7242 9789 (ICH switchboard) Fax: (+44) 020 7831 4366 E-mail: b.vernay(a)ich.ucl.ac.uk http://www.ucl.ac.uk/ich/services/lab-services/confocal_microscopy_core_fac…

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[FILM-Users 00047] Fwd: Cell Press Webinar: Forces In Cell Biology
by Martin Spitaler 16 Mar '11

16 Mar '11

...for your information: -------- Original Message -------- Subject: Cell Press Webinar: Forces In Cell Biology Date: Tue, 15 Mar 2011 22:37:23 +0000 From: Cell Press <cellpress(a)mail.cell-press.com> Reply-To: cellpress(a)mail.cell-press.com <cellpress(a)mail.cell-press.com> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Cell Press Announcing a new Cell Press Webinar ------------------------------------- Forces in Cell Biology Live date: Monday, April 4, 2011 12:00pm EST / 9:00am PST / 5:00pm GMT ------------------------------------- Register Today, It's Free... In this webinar, you'll hear from experts who are bridging the gap between biology and physics to look at forces inside the cell at the mitotic spindle, at the cell membrane during cell migration, and between cells within a tissue during embryonic morphogenesis. During the webinar, you will: - Hear from our panel of experts about how force applies to specific aspects of cell function. - Learn how the role of forces can be considered when approaching a biological problem. - Have the opportunity to ask questions of our panelists in real time. Register Today, It's Free... http://mediazone.brighttalk.com/event/ReedElsevier/ec16c57ed3-4938-intro Chemical and electrical signals are well known to contribute to the growth and development of an organism; recently, there has been an emerging focus on another cue that can inform cellular development physical force. Cells are pushed, pulled, and squeezed as they undergo biological processes such as cell division, migration, and morphogenetic events, and exciting recent work is identifying the role such forces play in governing cell function. While the mechanisms governing the dynamics of physical forces within the cell have not been exhaustively described, what is clear is that from the molecular to the organismal level force is a key aspect of biological function. In this webinar, we'll hear from experts who are bridging the gap between biology and physics to look at forces inside the cell at the mitotic spindle (Tarun Kapoor), at the cell membrane during cell migration (Gaudenz Danuser), and between cells within a tissue during embryonic morphogenesis (Jennifer Zallen). Unable to join us on April 4? This webinar will be archived for 6 months for on-demand viewing. Register todayto ensure you receive updates on how to gain access after the live event! Speakers: T. Kapoor Tarun Kapoor Ph.D. Pels Family Professor, Rockefeller University, New York G. Danuser Gaudenz Danuser Ph.D. Professor of Cell Biology, Harvard Medical School, Boston J. Zallen Jennifer Zallen Ph.D. Head of the Morphogenesis and Polarity Laboratory,Memorial Sloan-Kettering Cancer Center, New York Moderated by: Deborah Taylor, Current Biology Rebecca Alvania, Trends in Cell Biology Cell Press This email has been sent from Cell Press, a division of Elsevier Inc, 600 Technology Square, 5th Floor, Cambridge, MA 02139, USA, using smartFOCUSDIGITAL technology and platform. You are receiving this e-mail because you have previously expressed interest in announcements regarding Cell Press journals. If you no longer wish to receive messages of this type, please click here. For all inquiries, problems or suggestions regarding this service, please contact cellpress(a)cell.com. Copyright Â© 2011 Cell Press/Elsevier Inc. All rights reserved. Cell Press Privacy Policy| smartFOCUSDIGITAL Privacy Policy

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[FILM-Users 00046] CFP and YFP plasmids
by Martin Spitaler 15 Mar '11

15 Mar '11

Dear microscopists, could anyone help? Aurelia Magids (Ralph Knoell lab) needs some plain CFP and YFP plasmids as FRET controls. Ideally not linked to any protein. If you have a few micrograms of such constructs, please get in contact with her (copied in). Good imaging, Martin ######################################### Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00045] Fwd: FW: Promoterless Fluorescent Protein Vector
by Martin Spitaler 04 Mar '11

04 Mar '11

...can anyone help? -----Original Message----- From: infection-and-immunity-bounces(a)imperial.ac.uk [mailto:infection-and-immunity-bounces@imperial.ac.uk] On Behalf Of Laidlaw, Stephen M Sent: 04 March 2011 16:52 To: infection-and-immunity Subject: [Infection-and-Immunity] Promoterless Fluorescent Protein Vector Dear All Does anybody have any promoterless fluorescent protein vectors (e.g. those supplied by Clontech) that I could use? Thanks Steve -- Stephen M. Laidlaw PhD Section of Virology Department of Medicine Imperial College London St Mary's Campus Norfolk Place London W2 1PG UK tel: +44-(0)207-594-1927 fax: +44 (0)207-594-3973 email: s.laidlaw(a)imperial.ac.uk

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[FILM-Users 00044] FILM User Committee
by Martin Spitaler 03 Mar '11

03 Mar '11

Dear microscopists, it/'s been a while since our last FILM User Committee meeting, so to make sure everyone is happy and no unresolved problems are building up in the busy facility life, it would be good to meet again soon. First we need to fill up the committee though, several members have left the College. So if you are an active FILM user and would like participate in the organisation of FILM, please let me know, we need a few more people, particularly active users (although participation of PIs is of course always welcome!). The role of the user committee is to make sure the facility is organised as well as possible to everyone's satisfaction. It's not much work, we only meet around once a year (unless something urgent comes up). Issues discussed in the past are the rules set out in the User Guidelines (e.g. booking restrictions or cancellations), data handling (how soon old data can be deleted), best access to microscopes for long experiments etc. Current members are: * Graham Joyce (Brian Robertson lab) * Guy Rutter (PI) * Jennifer Frueh (Robert Krams lab) * Kate Hardy (PI) * Vania Braga (PI) If you could be convinced to participate, please let me know. Good imaging, Martin ############################################## Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00043] Fwd: Re: FW: postdoc position
by Martin Spitaler 02 Mar '11

02 Mar '11

For your information, a postdoc advert in imaging: Sebastian Barg used to work here at Imperial College before moving to Uppsala, and his work is all about imaging insulin secretion from pancreatic beta cells. -------- Original Message -------- Subject: Re: FW: postdoc position Date: Wed, 2 Mar 2011 10:01:36 +0000 From: Sebastian Barg <sebastian.barg(a)mcb.uu.se> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin, thanks! The positions are financed by a national strategic inititative in Diabetes (Lund and Uppsala) , so it is expected that the successful candidates work within the field or are willing to do so. There is a slight bonus for prior experience with islets, but this is not an absolute requirement. Experience with live cell imaging is definitely a plus. Yes, this could be in my lab - and there is a good chance to get the money if there is a suitable candidate (thanks to the short deadline and the missing english translation). I am nearly completely focused on imaging, here is my pitch http://www.mcb.uu.se/research/sbresearch.html with the details. I'd be happy to help anyone interested with the application, including the research plan and translation of the ad. Deadline is March 8. best regards, Sebastian > > > From: Sebastian Barg [mailto:sebastian.barg@mcb.uu.se] > Sent: 22 February 2011 09:12 > To: Loder, Merewyn > Subject: postdoc position > > > http://www.personalavd.uu.se/ledigaplatser/497fors.html > > > > below is the (lousy) Google translation from the Swedish original: > > > Uppsala University hereby declares the following position > Researchers at the postdoctoral level in intracellular signaling in islet cells > > within the strategic research collaboration EXODIAB > > Temporary employees and full time for one year with possibility of extension. To start immediately. > > Service holder will work under the guidance of a research group leader at the University of Uppsala, which is linked to the strategic research collaboration EXODIAB (Excellence of diabetes research in Sweden).The service is placed at the institution of the University of Uppsala where the research team leader operates.Applications from persons not already working in a EXODIAB-related projects. > > Project Description: One of EXODIAB Consortium's objectives are to identify the molecular mechanisms leading to type 2 diabetes. This disease is characterized by the inability of Î²-cells in pancreatic islets of Langerhans to compensate for increased insulin requirements in relation to insulin resistance. The reason for the insufficient Î²-cell function is largely unknown. A prerequisite for clarifying the mechanisms underlying the deterioration of the function of Langerhans island cells in diabetes is that the phenotype in normal function is well-characterized, in particular the intracellular signaling that mediates the effects of nutrients, hormones and nerve transmitters in the release of Langerhans island hormones. For the service, applications with projects focusing on issues regarding the intracellular signaling that regulate hormone secretion in Langerhans island cells to be considered. > > Duties: Service holder shall mainly engage in research within a research group, and with independent projects and in collaborative projects with other research groups.Some of the teaching and administration may possibly be seen (max 20%). > > Qualifications: The applicant must have a doctorate in cell biology, physiology or other relevant field. Particular emphasis is given to scientific excellence in the field.Previous experience of research on the islets of Langerhans is an advantage. It is an advantage if applying for a project involving cooperation between diabetes researchers in the University of Uppsala or between Uppsala and Lund University. The application must include a proposal for a project that can be performed in research collaboration EXODIAB. Emphasis will be placed on personal ability and teamwork skills. > > Salary: Salaries for individual qualifications. Please state salary in the application. > > Contact: Management of EXODIAB at Uppsala University: > Professor Bryndis Birnir, Department of Neuroscience, Uppsala University, Biomedical Center, Box 593, 75 124 Uppsala. Tel: 018-471 46 22, E-mail: bryndis.birnir @ neuro.uu.se > Professor Olle Korsgren, Department of Clinical Immunology, Rudbeck Laboratory, Uppsala University Hospital, 751 85 Uppsala, Sweden. Tel: 018-611 41 87, E-mail: olle.korsgren @ klinimm.uu.se > Associate Professor Anders Tengholm, Department of Medical Cell Biology, Uppsala University, Biomedical Center, Box 571, 75 123 Uppsala. Tel: 018-471 44 81, E-mail: anders.tengholm @ mcb.uu.se > > Union representatives are Anders Grundmann, Saco Council, tel. 018-471 53 80, Carin SÃ¶derhÃ¤ll, TCO / ST, tel. 018-471 19 96 alt. 018-471 1997 Stephen DjurstrÃ¶m, SEKO, tel. 018-471 33 15th > > Application: The application must include a curriculum vitae, publication list and a research plan for the proposed project (maximum two pages). If possible, name, address, telephone number and e-mail to the current research group leader in the application. > > Please submit your application no later than 8 March 2011, UFV-PA 2010/497. Click on the link below to get the application form. > > Researchers at the postdoctoral level in intracellular signaling in Langerhans island cells > > | up | > > > > Short info > > Application deadline: > > 2011-03-08 > > Registration number: > > UFV-PA 2011/497 > > Â© -2004. UPPSALA UNIVERSITY, Box 256, 751 05 Uppsala > Updated: 2011-02-22 | Editor | Webmaster > > > > -- > > _____________________________________________ > > > > Sebastian Barg, PhD > > Medical Cell Biology > > Uppsala University > > BMC box 571 > > 75123 Uppsala, Sweden > > +46-4714660 > > -- _____________________________________________ Sebastian Barg, PhD Medical Cell Biology Uppsala University BMC box 571 75123 Uppsala, Sweden +46-4714660

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