Dear microscopists,
we have now a set of Fura-2 filters available on Widefield 2. If you
want to use them, please get in contact for help with the correct settings.
Good imaging,
Martin
--
------------------------------------------------------------------------
*Martin Spitaler, PhD*
*FILM - Facility for Imaging by Light Microscopy*
- Facility Manager -
Sir Alexander Fleming Building, desk 401
Imperial College London / South Kensington
Exhibition Road
London SW7 2AZ
UK
Tel. +44-(0)20-759-42023
E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk>
Website: http://imperial.ac.uk/imagingfacility
-------- Original Message --------
Subject: Imaging Postdoc
Date: Fri, 4 Feb 2011 11:25:21 +0000
From: Nick Barry <nbarry(a)mrc-lmb.cam.ac.uk>
Dear Colleague
I am contacting you to ask if you might know of any recently graduated
PhDs or Postdocs looking for a position in an imaging facility. At the
LMB we have a 3 year position for someone working on microscopy and
imaging techniques, in particular superresolution techniques. Whilst it
is envisaged that much of this work will be by collaboration with
individual research groups within the LMB there is also the expectation
that time will be spent in support of overall microscopy services at the
lab.
Details may be found by following the link from
http://www.mrc.ac.uk/About/WorkingfortheMRC/Vacancies/index.htm
The reference number for this vacancy is IRC12266. The listing will
appear on the 'Jobs.ac.uk' and 'nature jobs' websites.
If you have any candidates who might suit this role i would be grateful
if you could pass on this message.
Many regards
Nick Barry
MRC Laboratory of Molecular Biology
Cambridge
Dear microscopists,
I'm very pleased to announce that we now have a fully functional TIRF
microscope available. The microscope is an adaptation of a manual Zeiss
Axiovert 200 system, with addition of a Till monochromator for standard
fluorescence excitation, two lasers (491 and 561nm) for TIRF imaging, a
high-resolution TIRF objective (100x/1.45), a high-sensitivity camera
(Hamamatsu C9100-13) and an Optosplit for parallel visualisation of
green and red fluorescence.
The microscope is available from now, but because of the manual setup
interested users need to attend the laser safety course before they can
get training.
For more detailed information, see EQUIPMENT page on the FILM website
/ Widefield 3 - TIRF.
And as an outlook for the not-too-distant future: We have also been
working hard to implement PALM and STORM microscopy on the same system,
and have now achieved preliminary results with both techniques. Several
optimisation still need to be done, but hopefully we will be able to do
high-resolution microscopy in all three dimensions (TIRF for 'Z' and
PALM / STORM for 'XY') very soon.
Good imaging,
Martin
TIRF microscope: <http://www3.imperial.ac.uk/imagingfacility/equipment/wf3>
TIRF microscopy:
<http://www3.imperial.ac.uk/imagingfacility/links/imaging_information>
###################################################
Martin Spitaler, PhD
FILM - Facility for Imaging by Light Microscopy
- Facility Manager -
Sir Alexander Fleming Building, desk 401
Imperial College London / South Kensington
Exhibition Road
London SW7 2AZ
UK
Tel. +44-(0)20-759-42023
E-mail m.spitaler(a)imperial.ac.uk
Website: http://imperial.ac.uk/imagingfacility