February 2011 - FILM-Users

[FILM-Users 00041] damaged objective
by Martin Spitaler 25 Feb '11

25 Feb '11

Dear microscopists, unfortunately, on WF2 the 63x oil immersion objective has been damaged again, the front lens has been pushed in. Everybody, please be extremely careful with objective lenses, especially when changing stage inserts or moving motorised stages. Last time this happened, the repair cost over £2,500, if irreparable, a new lens of this quality would cost £6,000-8,000!! So please, however urgent your experiments, be extremely careful with the equipment. And while this lens is being repaired, the 63x oil objective needs to be shared between WF2 and CF5. Please send a message to SHAREPOINT HARDWARE REQUESTS a day in advance if you want to use it, and do NOT move it around yourself. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00038] TOMORROW: FILM Club - Judy Fonville on hyperspectral mass-spec imaging data analysis
by Martin Spitaler 25 Feb '11

25 Feb '11

Dear microscopists, tomorrow, Friday, 25th of February, is our next FILM Club. Judy Fonville (Jeremy Nicholson lab) is showing us how she analyses hyperspectral mass spectrometry imaging data to extract a maximum amount of information in an unbiased manner. Judy works in metabolomics, but for her projects uses data generated by an interesting combination of imaging and mass spectrometry. Apart from the specific scientific background, her technique of analysing multi-dimensional data with unknown structure can also be interesting for all of us trying to understand multi-dimensional imaging data like those from a Flim microscope or high-resolution microscopy. The FILM Club is on Friday 25 Feb 1pm, SAFB 121. Good imaging, Martin http://www3.imperial.ac.uk/imagingfacility/news ############################################ Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility _______________________________________________ FILM-Users mailing list FILM-Users(a)imperial.ac.uk https://mailman.ic.ac.uk/mailman/listinfo/film-users

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[FILM-Users 00039] requesting microscope hardware (objectives, lenses)
by Martin Spitaler 24 Feb '11

24 Feb '11

Dear microscopists, because we have more and more parts which are used on multiple microscopes, like special objectives and filter cubes, we are introducing a FILM HARDWARE REQUEST message system: From now on, if you need any special bits of equipment like special objectives, filter cubes etc. - anything that's listed in the FILM WIKI as ON REQUEST, please go to the FILM SHAREPOINT BOOKING page and send a message to FILM HARDWARE REQUESTS. We will then make sure everything is in place when you start your session. This system is also intended as a notification system for other users using the same bits of hardware. To make this work, please subscribe to these messages: On the FILM SHAREPOINT BOOKING page, in the left side-bar under DISCUSSIONS click on FILM HARDWARE REQUESTS, then in the menu go to ACTIONS > ALERT ME. If more than one person requests the same equipment, the first person to ask will get it, obviously. The same two-week rule for booking in advance as for all other booking applies. If you have any problems with this system, please let us know. Good imaging, Martin FILM SHAREPOINT BOOKING: <https://sharepoint.ic.ac.uk/FoM/NHLI/Facilities/FILM/default.aspx> FILM HARDWARE REQUESTS: <https://sharepoint.ic.ac.uk/FoM/NHLI/Facilities/FILM/Lists/FILM%20Hardware%…> ############################################################# Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00036] FWD: Postdoc position
by Martin Spitaler 23 Feb '11

23 Feb '11

-------- Original Message -------- Date: Tue, 22 Feb 2011 12:18:00 +0000 From: Purbhoo, Marco <marco.purbhoo(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin I’d be grateful if you could circulate below Post-doc vacancy amongst the imaging users. Many thanks Marco https://www4.ad.ic.ac.uk/OA_HTML/OA.jsp?akRegionCode=IRC_VIS_VAC_DISPLAY_PA… <https://www4.ad.ic.ac.uk/OA_HTML/OA.jsp?akRegionCode=IRC_VIS_VAC_DISPLAY_PA…>.. _______________________________________________________ Marco Purbhoo, D. Phil. Lecturer in Hepatology Imperial College London / St. Mary’s Campus Section of Hepatology & Gastroenterology Division of Diabetes, Endocrinology & Metabolism Department of Medicine Variety Wing, Room VE7 Norfolk Place, London W2 1PG United Kingdom Tel: +44 207 5940832

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[FILM-Users 00035] Sharepoint booking - confusing cancellations
by Martin Spitaler 23 Feb '11

23 Feb '11

Dear microscopists, when deleting bookings, some of you reduce the time to '5 min' instead of '0 min'. Unfortunately, this means that the booking in the standard view of Sharepoint appears as if it were for half an hour, and users have mistakingly left microscopes on because they thought someone has booked it afterwards. Could you please reduce the time to zero instead (same start and end time)? The booking will disappear from the standard DIARY CALENDAR VIEW, but it's still visible in the LIST VIEW (click on the yellow DIARY CALENDAR VIEW icon on the top-left of the calendar to change the view). Thanks, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00034] FILM Club next Friday
by Martin Spitaler 18 Feb '11

18 Feb '11

Dear microscopists, next Friday, 25th of February, is our next FILM Club. Judy Fonville (Jeremy Nicholson lab) is showing us how she analyses hyperspectral mass spectrometry imaging data to extract a maximum amount of information in an unbiased manner. Judy works in metabolomics, but for her projects uses data generated by an interesting combination of imaging and mass spectrometry. Apart from the specific scientific background, her technique of analysing multi-dimensional data with unknown structure can also be interesting for all of us trying to understand multi-dimensional imaging data like those from a Flim microscope or high-resolution microscopy. The FILM Club is on Friday 25 Feb 1pm, SAFB 121. Good imaging, Martin http://www3.imperial.ac.uk/imagingfacility/news ############################################ Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00025] FILM Image Of The Year 2011 competition
by Martin Spitaler 18 Feb '11

18 Feb '11

Dear microscopists, sorry for the delay, one of the judges pulled out because of too much work, but now it's up again, our FILM IMAGE OF THE YEAR 2011 competition. The first prize is again a compact camera sponsored by Invitrogen, and the top 12 entrants will again receive a beautiful A3 calendar! So don't hesitate, dig out your best images and make the best of them. The EXTENDED DEADLINE is Friday 18 February. For the rules, and how to participate, have a look at the FILM website under GALLERY <http://imperial.ac.uk/imagingfacility/research/gallery/film_image_of_the_ye…> Good imaging, and good luck! Martin ########################################### Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00032] Fwd: Bioinformatics 2010 seminar series - Feb 21 & 28th
by Martin Spitaler 17 Feb '11

17 Feb '11

...for your information - particularly the second talk could be interesting for microscopists... -------- Original Message -------- Subject: Bioinformatics 2010 seminar series - Feb 21 & 28th Date: Wed, 16 Feb 2011 15:25:06 +0000 From: Bioinformatics <bioinformatics(a)imperial.ac.uk> All are welcome to the following Centre for Bioinformatics seminars in SAF (Sir Alexander Fleming) building: Monday 21st February, 5pm G34 Lecture Theatre, SAF building Prof Richard Houlston, Institute of Cancer Research “What have association studies taught us about inherited predisposition to cancer?” http://www.icr.ac.uk/research/research_profiles/2761.shtml Monday 28th February, 4.30pm Rm 122, SAF building Prof Kevin Burrage, University of Oxford. “The Visible Cell'' http://www.sysbio.ox.ac.uk/People/KB.html Refreshments will be provided after the seminars. Please see attached flyers for more detail. Regards, Louise ********************************************* Centre for Bioinformatics (Post to:- Biochemistry Building) Division of Molecular Biosciences Faculty of Natural Sciences Imperial College London SW7 2AZ,UK bioinformatics(a)imperial.ac.uk www.imperial.ac.uk/bioinformatics (Centre) Tel +44 (0)20 7594 5715; Fax +44 (0)20 7594 5789 ****************************************************************

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[FILM-Users 00030] Fwd: OMERO in Action - Meeting - April 2011
by Martin Spitaler 09 Feb '11

09 Feb '11

-------- Original Message -------- Subject: OMERO in Action - Meeting - April 2011 Date: Wed, 9 Feb 2011 10:17:52 +0000 From: Jerome Avondo <jerome.avondo(a)BBSRC.AC.UK> Reply-To: Jerome Avondo <jerome.avondo(a)BBSRC.AC.UK> To: UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK <UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK> Hi all, This is a reminder that registration closing date for the*OMERO in action meeting* is approaching. *Registration closes on the 18^th of February 2011.* So if you are interested in attending/participating in the -*OMERO Lectures* (FREE) / 6^th April 2011 -*OMERO workshop* / 7^th -8^th April 2011 Please visit the registration site and book your place as there are only a few places left. Further information about the events can also be found on the website:*http://www.jic.ac.uk/events/omero/* Kind Regards, Jason Swedlow and Jerome Avondo. -- UK-EUROBIOIMAGING Visit the website http://www.bioimaginguk.org To e-mail the group, send messages to UK-EUROBIOIMAGING-PROJECT(a)JISCMAIL.AC.UK To subscribe visit https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=UK-EUROBIOIMAGING-PROJECT --

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[FILM-Users 00029] food and drinks in microscope rooms
by Martin Spitaler 09 Feb '11

09 Feb '11

Dear microscopists, unfortunately I have to remind you again that all our microscope rooms are lab space, and no food and drinks are allowed there. And there is good reason for it, most users do use all kinds of biological samples, transfections, oncogenes etc., and many of the nuclear dyes and fixatives are mutagenic. The worst idea is certainly to bring coffee cups along and through them into the clinical waste bin afterwards! So in everybody's interest, please stick to the rules in the future. Good imaging, Martin ################################################ Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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