October 2011 - FILM-Users - imperial.ac.uk

[FILM-Users 00120] Zeiss widefield demonstration
by Martin Spitaler 14 Dec '11

14 Dec '11

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[FILM-Users 00132] Fwd: FW: Optical Imaging Meeting, 3rd November
by Martin Spitaler 03 Nov '11

03 Nov '11

...forwarded for your information, should be interesting for many microscopists, Martin -------- Original Message -------- Subject: FW: Optical Imaging Meeting, 3rd November Date: Fri, 28 Oct 2011 16:20:07 +0100 From: Harding, Sian E <sian.harding(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hi Martin, May I ask you to circulate this through the FILM list please? Best regards Sian Optical Imaging Meeting Thursday 3^rd November MDL2-Bay D, Sir Alexander Fleming Building, South Kensington Campus 10:00 Godfrey Smith, University of Glasgow Multiscale functional optical imaging: the biologist’s experience 10:45 Paul French & Chris Dunsby, Imperial College London Photonics Group Techniques and technologies for functional imaging: the photonic perspective

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[FILM-Users 00134] Fwd: Volocity User Training
by Martin Spitaler 31 Oct '11

31 Oct '11

...forwarded, for your information, Martin -------- Original Message -------- Subject: Volocity User Training Date: Fri, 28 Oct 2011 02:00:15 -0400 From: PerkinElmer <perkinelmer(a)perkinelmer.com> Reply-To: PerkinElmer <perkinelmer(a)perkinelmer.com> To: <m.spitaler(a)imperial.ac.uk> Mobile-Friendly version <http://now.eloqua.com/e/es.aspx?s=643&e=123818&elq=124495b3f7ee4da8904ac83c…> Trouble Viewing? Read the online version <http://now.eloqua.com/e/es.aspx?s=643&e=123818&elq=124495b3f7ee4da8904ac83c…> Volocity User Training *Volocity User Training* Join our expert imaging team and learn how to realize the power of your Volocity^Â® 3D Image Analysis Software at our 1 day courses, given by our expert team in specialist software training facilities. Attend one or both days depending on your needs. Refer a Colleague <http://web4.perkinelmer.com/?elqPURLPage=5732&elq=124495b3f7ee4da8904ac83c5…> *Day 1: Volocity Essentials* *Date: November 8th Location: London, UK **Price: Â£300. Receive a Â£50 discount if you book a two day training package** Designed for new lab members, infrequent users or those in need of a refresher, this 1 day course will give you the skills to confidently explore your cellular images in 3D using Volocity 3D Image Analysis Software. * Get started with Volocity * Explore, publish and share 3D data *Course content: *This course provides an introduction to: * 3D rendering and publication * Image restoration * Volume measurements * Colocalization *Day 2: Volocity Quantitation* *Date: November 9th Location: London, UK Price: Â£300. Receive a Â£50 discount if you book a two day training package** If you're seeking to expand your skills or to resolve a specific problem this 1 day course will give you the skills to answer complex biological questions using Volocity 3D Image Analysis Software. * Improve your productivity * Answer more complex analysis questions * Apply your learning to your own data *Course content:* This course provides training in: * Volume measurements * Tracking * Trend analysis * Charting FIND A COURSE <http://web4.perkinelmer.com/MartinSpitaler4680?elqPURLPage=5731&elq=124495b…> LEARN MORE <http://web4.perkinelmer.com/MartinSpitaler4680?elqPURLPage=5686&elq=124495b…> FIND A COURSE <http://web4.perkinelmer.com/MartinSpitaler4680?elqPURLPage=5731&elq=124495b…> LEARN MORE <http://web4.perkinelmer.com/MartinSpitaler4680?elqPURLPage=5686&elq=124495b…> Volocity User Training *Cancellation & Refund Policy * Please note that PerkinElmer may cancel or reschedule courses in the event of unforeseen circumstances or if the minimum enrolment is not met. In these circumstances registered students will be offered a choice to apply their training tuition to an alternative Volocity training program or, alternatively, to receive a full refund of course fees paid to PerkinElmer. We recommend you take this into account when making travel arrangements as PerkinElmer is not responsible for any of your expenses or losses, whether for travel or otherwise, incurred in the event of cancellation by PerkinElmer for any reason. *Student Refund Policy * If you notify us in writing of your cancellation up to 15 business days prior to the course date â Full refund of course registration fees paid Up to 5 business days prior to the course â 50% refund of course registration fees paid No refunds will be made if cancellation is made within five business days of the course. All cancellations must be made in writing to the training administrator directly, details will be included in your course confirmation documents. * Payment* Payment is due upon registration through our e-commerce site (PO and credit cards accepted). Â© 2011 PerkinElmer, Inc. All rights reserved. PerkinElmer^Â® is a registered trademark of PerkinElmer, Inc. All other trademarks are property of their respective owners. PerkinElmer, Inc. | 940 Winter Street | Waltham, MA 02451 USA | www.perkinelmer.com <http://app.reply.perkinelmer.com/e/er.aspx?s=643&lid=5&elq=124495b3f7ee4da8…> Manage <http://web.perkinelmer.com/MartinSpitaler4680?elqPURLPage=5472&elq=124495b3…> your PerkinElmer Life Sciences eNews Subscriptions. You are currently subscribed as m.spitaler(a)imperial.ac.uk. Unsubscribe <http://web.perkinelmer.com/MartinSpitaler4680?elqPURLPage=5829&elq=124495b3…>from all automated communications from PerkinElmer, Inc. Follow us on Facebook <http://www.facebook.com/PerkinElmer?elq=124495b3f7ee4da8904ac83c5b651f4c>twitter-button-small.gif <http://twitter.com/PKILifeScience?elq=124495b3f7ee4da8904ac83c5b651f4c>

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[FILM-Users 00130] FILM Club this Friday: super-resolution microscopy in the facility
by Martin Spitaler 28 Oct '11

28 Oct '11

Dear microscopists, this weeks FILM CLUB will all be about SUPER-RESOLUTION MICROSCOPY done in the facility: Steve Rothery (FILM) will introduce PALM and STORM, our microscope setup (WF3) and the requirements for samples (fluorophores) to make it work. Sophie Pageon (Dan Davis lab) will then show on her data how this technology can be used to get an insight into molecular organisation of a signalling molecule. The FILM Club will be this Friday, 28 October 2011, 13.00 in seminar room SAFB-120. Yet again, this promises to be very interesting, don't miss out. See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00127] Volocity Server
by Scott, Mark 18 Oct '11

18 Oct '11

Dear FILM Users, We have now started running a new Volocity licence server as the old one was removed due to a hardware upgrade and to keep the server running we needed to find a new server. As such the old details will not work for users. If you require access to Volocity please get in contact with either myself, Martin or Steve and we will add you to the system and send you the details of the new server. Thank you Mark Scott FILM - Facility for Imaging by Light Microscopy Senior Research Technician Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel: ++44(0)20-759-49793 E-mail: mark.scott1(a)imperial.ac.uk<mailto:mark.scott1@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00126] Reminder: Reminder: Zeiss widefield demonstration
by Martin Spitaler 17 Oct '11

17 Oct '11

Dear microscopists, this is a reminder that this week we are having the demonstration / testing of the Zeiss widefield system. The microscope is available from tomorrow until Thursday night to test it with your own samples. It offers various high-sensitivity cameras, a fast-switching (msec) LED illumination system for fast multicolour / ratiometric imaging and a Vivatome for fast 3D / confocal-like imaging. Please book your time slot now, places typically fill up quickly. And switching between the various available hardware takes a bit of time, so we need to plan the schedule in advance. The setup is installed in SAFB-530. See you there, good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00125] problem with Volocity licence server
by Martin Spitaler 14 Oct '11

14 Oct '11

Dear microscopists, we are having a problem with the Volocity licence server and need to reinstall it. I hope we'll get it back up and running some time today. Sorry for the troubles. Good imaging, Martin -- ------------------------------------------------------------------------ *Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00123] Hardware Requests
by Scott, Mark 13 Oct '11

13 Oct '11

Dear Users, Many of you are already aware of and using the hardware request function to reserve filters and lenses for the microscopes in the facility. I just want to clarify the lens situation and that there are two lists of lenses in the drop down list - 1 for Zeiss lenses and 1 for Leica. The lenses are pre-fixed with a "Z" or an "L" respectively. Please ensure you select the correct lenses for the microscope you are booking (i.e. Leica lenses for a Leica microscope) to guarantee the lens you request will actually fit in the microscope you have booked. As always any questions please forward them to a member of the FILM staff. Thank you Mark Scott FILM - Facility for Imaging by Light Microscopy Senior Research Technician Sir Alexander Fleming Building, desk 408 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel: ++44(0)20-759-49793 E-mail: mark.scott1(a)imperial.ac.uk<mailto:mark.scott1@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00122] Fwd: FW: UK poster
by Martin Spitaler 12 Oct '11

12 Oct '11

...forwarded, for your information... Martin -------- Original Message -------- Subject: FW: UK poster Date: Mon, 10 Oct 2011 18:37:47 +0100 From: French, Paul (PHOT) M W (Photonics) <paul.french(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk>, Nadal, Rebecca C <r.nadal(a)imperial.ac.uk> Thursday, October 13th, 2011, 5:00 pm Venue: Greenwood Lecture Theatre, Guy's Campus, King's College London Leica Scientific Forum London Professor Taekjip Ha, University of Illinois at Urbana-Champaign, US: “Single Molecule Biology: One, two, three and four“ 5:00 Welcome & Introduction of speaker by chairman Simon Ameer-Beg Taekjip Ha will talk about: • Recent tool developments in single molecule nanometry where a position of a single fluorescent molecule can be determined with a single nanometer precision • How these new tools provide fundamental insights on how molecular motors move on cellular highwayscombining optical tweezers that can apply and measure pico-Newton forces to single biological molecules with single molecule FRET • Latest studies on complex processes that require coordination between multiple proteins, such as DNA replication, using multi-dimensional single molecule measurement techniques 6:15 Discussion & post lecture reception Scientific Advisory Board: Prof. Paul French (Imperial College London), Prof. Antony Galione (Oxford Univ.), Dr. Jim Haseloff (Cambridge Univ.), Prof. David Klenerman (Cambridge Univ.), Prof. Gero Miesenboeck (Oxford Univ.), Prof. Tony Ng (King’s College London), Prof. Alexei Tepikin (Liverpool Univ.), Prof. Mike White (University of Manchester), Prof. Keith Willison (Institute of Cancer Research London), Prof. Tony Wilson (Oxford Univ.), and Dr. Thomas Zapf (Leica Microsystems) For free registration please e-mail to: lsf(a)leica-microsystems.com Learn more: http://www.leica-microsystems.com/events/leica-scientific-forum/lsf-details…

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[FILM-Users 00121] Fwd: Can you please circulate this to the mailing list?
by Martin Spitaler 12 Oct '11

12 Oct '11

...forwarded, for your information. Attachments don't work with the Mailmanager, but all information is below (just without pictures)... Martin -------- Original Message -------- Subject: Can you please circulate this to the mailing list? Date: Wed, 12 Oct 2011 12:30:21 +0100 From: Konitsiotis, Antonios D <antonios.konitsiotis05(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> Hey Martin, I have attached a poster and the info about the Perkin-Elmer visit next week. Can you please circulate what you can to the FILM users and whoever else will be interested? Thank you! All the best, Antonio */Dr. Antonio D Konitsiotis/* Research Associate Department of Molecular Medicine/NHLI Imperial College London, South Kensigton Campus, 2nd Floor, Desk 7, Sir Alexander Fleming Building, London, SW7 2AZ U.K. e-mail: antonios.konitsiotis05(a)imperial.ac.uk <blocked::mailto:antonios.konitsiotis05@imperial.ac.uk> mob: +44 7912552947 ** *Perkin Elmer visit: ‘Introduction to the Operetta High Content Screening System and Harmony software’* *20^th of October 12pm-1pm, seminar room 122 in the SAF building.* *Everyone is welcome.* ** Perkin Elmer will be on campus on the 20^th of October to talk about their new high throughput systems. The speaker is Hans Pirard and he is European High contents screening specialist and he will give an hour presentation on the operetta High content screening system and columbus image data management solutions. In his talk he will give an overview of the technologies and then actual examples of uses of the system on different cell types. He will show how analysis can be performed with harmony software using patented PhenoLOGIC and machine learning technology which allows you to teach the operetta to recognise and distinguish between sub populations of cells as you can by eye. After the seminar Hans will be available to discuss further about the use of these systems in your own experiments. For more information about the power of these new tools please click on links to the Operetta systems (http://www.perkinelmer.com/pages/020/cellularimaging/products/operetta.xhtml) and the Columbus software (http://www.perkinelmer.com/pages/020/cellularimaging/products/columbus.xhtml)

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