January 2011 - FILM-Users - imperial.ac.uk

[FILM-Users 00024] offline computer working again
by Martin Spitaler 31 Jan '11

31 Jan '11

Dear microscopists, our offline computer has been re-installed with Windows-7 and is up and running again. Volocity and Definiens have already been reinstalled, if you want to use other software please get in contact so we can do so. If you are going to use it, please use your normal College login for access, but store all data in the C:\USER folder - not(!!) your desktop; that makes our life much easier cleaning up the harddrive when it fills up. And the Volocity licence server had some problems recently, but should be up and running again now. Good imaging, Martin ############################# Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00023] Fwd: [neuroscience-technology-network] FW: Talk by Thomas Knopfel at UCL on 2nd February
by Martin Spitaler 31 Jan '11

31 Jan '11

-------- Original Message -------- Subject: [neuroscience-technology-network] FW: Talk by Thomas Knopfel at UCL on 2nd February Date: Tue, 11 Jan 2011 19:26:24 +0000 From: Harris, Kenneth D <kenneth.harris(a)imperial.ac.uk> To: neuroscience-technology-network <neuroscience-technology-network(a)imperial.ac.uk> Hi everyone, I would like to pass on information about a special seminar, organized by Matteo Carandini at UCL. "Towards an optogenetic electrophysiology" Thomas Knopfel RIKEN, Japan 2 February 2011, 4 pm J Z Young Lecture Theatre Anatomy Building, Gower Street University College London Dr. Knopfel is one of the main developers of a transformative new technology: voltage sensitive fluorescent proteins. His work received very considerable attention at the last SfN meeting. All the best, Kenneth. _______________________________________________ neuroscience-technology-network mailing list neuroscience-technology-network(a)imperial.ac.uk https://mailman.ic.ac.uk/mailman/listinfo/neuroscience-technology-network

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[FILM-Users 00022] Fwd: FW: search for an engineer or postdoctoral researcher
by Martin Spitaler 28 Jan '11

28 Jan '11

FYI -------- Original Message -------- Subject: FW: search for an engineer or postdoctoral researcher Date: Wed, 26 Jan 2011 09:17:24 +0000 From: Buck, Martin <m.buck(a)imperial.ac.uk> To: Spitaler, Martin <m.spitaler(a)imperial.ac.uk> ------ Forwarded Message *From: *"A.J. Carpousis" <Agamemnon.Carpousis(a)ibcg.biotoul.fr> *Date: *Wed, 26 Jan 2011 07:47:34 -0000 *Subject: *search for an engineer or postdoctoral researcher Hello: Please find in the attached PDF a job offer. I am trying to recruit someone with expertise in fluorescence microscopy. I would appreciate it if you forwarded the PDF to potential candidates. Many thanks in advance. Best wishes. A.J. Carpousis <https://fileexchange.imperial.ac.uk/files/6b9f543c9b/Research%20in%20Cellul…> ------ End of Forwarded Message

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[FILM-Users 00020] correction: FILM CLUB next Friday: Omero
by Martin Spitaler 28 Jan '11

28 Jan '11

thanks to those who have noticed, the correct date is of course FRIDAY 28 JAN 2011! Dear microscopists, in the FILM CLUB next Friday (FRIDAY 28 JAN 2011, 1pm, SAFB 121) myself and Mark Woodbridge / Chris Tomlinson will introduce the OMERO image database, which is now tested, running and ready for use - and even completely free of charge! Over the last two years, FILM in collaboration with the Bioinformatics Support Service (Mark and Chris) have been busy with installation, testing and feedback to the developers, now we can finally announce that the OMERO Image Database is ready and available for you to use. This database allows a complete workflow for image data storage, annotation and documentation, analysis and visualisation: * original image data from the different microscopes are imported into OMERO, and both the image content (pixels) and the metadata (microscope, objective, magnification, filters, etc.) are extracted and stored in the database * irrespective of the original file type, images and metadata are stored in a standardised format (based on OME-TIFF) * images are stored on a central, backed-up server and accessible from everywhere * images, movies, 3D stacks and XYZT series can be visualised with various rendering settings * datasets can be annotated and searched * images can be shared with other people * images, search results and summaries can be exported Already the export of various microscopy data in the standardised OME-TIFF format makes use and exchange of analysis tools far easier. We will also talk about our plans to streamline the workflow from acquisition to analysis and visualisation, e.g. directly linking OMERO to image analysis in Definiens or ImageJ, streaming data from Leica microscopes directly onto the server, integration with Chris' XperimentR etc. Links for further information can be found on our NEWS website <http://www3.imperial.ac.uk/imagingfacility/news> Good imaging, Martin ########################################################## Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00019] FILM CLUB next Friday: Omero
by Martin Spitaler 21 Jan '11

21 Jan '11

Dear microscopists, in the FILM CLUB next Friday (18 Jan 2011, 1pm, SAFB 121) myself and Mark Woodbridge / Chris Tomlinson will introduce the OMERO image database, which is now tested, running and ready for use - and even completely free of charge! Over the last two years, FILM in collaboration with the Bioinformatics Support Service (Mark and Chris) have been busy with installation, testing and feedback to the developers, now we can finally announce that the OMERO Image Database is ready and available for you to use. This database allows a complete workflow for image data storage, annotation and documentation, analysis and visualisation: * original image data from the different microscopes are imported into OMERO, and both the image content (pixels) and the metadata (microscope, objective, magnification, filters, etc.) are extracted and stored in the database * irrespective of the original file type, images and metadata are stored in a standardised format (based on OME-TIFF) * images are stored on a central, backed-up server and accessible from everywhere * images, movies, 3D stacks and XYZT series can be visualised with various rendering settings * datasets can be annotated and searched * images can be shared with other people * images, search results and summaries can be exported Already the export of various microscopy data in the standardised OME-TIFF format makes use and exchange of analysis tools far easier. We will also talk about our plans to streamline the workflow from acquisition to analysis and visualisation, e.g. directly linking OMERO to image analysis in Definiens or ImageJ, streaming data from Leica microscopes directly onto the server, integration with Chris' XperimentR etc. Links for further information can be found on our NEWS website <http://www3.imperial.ac.uk/imagingfacility/news> Good imaging, Martin ########################################################## Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00018] TODAY: Leica Matrix demonstration
by Martin Spitaler 19 Jan '11

19 Jan '11

A reminder about today's presentation and demonstration: -------- Original Message -------- Subject: Leica Matrix demonstration Date: Fri, 14 Jan 2011 11:58:48 +0000 From: Martin Spitaler <m.spitaler(a)imperial.ac.uk> To: film-users <film-users(a)imperial.ac.uk> CC: Colin Park <colin.Park(a)leica-microsystems.com>, Paul McCormick <paul.mccormick(a)leica-microsystems.com> Dear microscopists, for next week Wednesday I have organised a DEMONSTRATION and trial of the LEICA MATRIX SOFTWARE. This software would allow the automation of our confocal microscopes and could have some important advantages for us: * ability for flexible design of screening experiments, e.g. automated tiling of multiple areas or wells * automated focussing on the bottom of a coverslip using laser reflection * direct streaming of data onto a data server using the OME-TIFF format, hopefully allowing full integration with our OMERO image database, thus avoiding problems with data import, unnecessary transfer of large data files, etc. * potential for on-the-fly analysis feeding back to the image acquisition (e.g. fast low-resolution screen, detailed acquisition in wells with e.g. high expression level) For more details, see the Leica website at <http://www.leica-microsystems.com/products/confocal-microscopes/details/pro…> SCHEDULE FOR LEICA MATRIX DEMONSTRATION, Wednesday 19 January 2011: 12.00: Presentation of the Leica Matrix Platform by Paul McCormick (Leica Support Specialist), seminar room SAFB-122 14.00: Hands-on demonstration and mini training on the inverted Leica-SP5 ('confocal 4') Everyone is welcome, but if you are interested in the hands-on session please let me know, there is limited space at CF4. After the demonstration, we will keep and test a trial version for several weeks. If successful, we'll try to put together a grant application with all interested parties. Good imaging, Martin ################################################## Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00017] imaging register
by Martin Spitaler 17 Jan '11

17 Jan '11

Dear microscopists, our facility hopefully covers all your light microscopy needs (we are just about to introduce our TIRF microscope, and we are getting closer to STORM / PALM), but if you need other imaging techniques (EM, MRI, PET/SPECT, X-ray, ultrasound, ...) you might found the IMAGING REGISTER useful. New people might not be aware of this, but it is an extensive, searchable resource of equipment, people, expertise,... related to imaging. Whether you are looking for paramagnetic fluorescent multimodal lipids or a Dendra2 plasmid, a TEM or a collaborator for your project, this could be a useful resource. To find it, go to <http://web.bioinformatics.ic.ac.uk/imaging> or follow the link on the FILM website / LINKS / IMPERIAL Good imaging, Martin * ################################## Martin Spitaler, PhD* *FILM - Facility for Imaging by Light Microscopy* - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk <mailto:m.spitaler@imperial.ac.uk> Website: http://imperial.ac.uk/imagingfacility

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[FILM-Users 00016] Fwd: [neuroscience-technology-network] FW: Talk by Thomas Knopfel at UCL on 2nd February
by Martin Spitaler 14 Jan '11

14 Jan '11

FYI -------- Original Message -------- Subject: [neuroscience-technology-network] FW: Talk by Thomas Knopfel at UCL on 2nd February Date: Tue, 11 Jan 2011 19:26:24 +0000 From: Harris, Kenneth D <kenneth.harris(a)imperial.ac.uk> To: neuroscience-technology-network <neuroscience-technology-network(a)imperial.ac.uk> Hi everyone, I would like to pass on information about a special seminar, organized by Matteo Carandini at UCL. "Towards an optogenetic electrophysiology" Thomas Knopfel RIKEN, Japan 2 February 2011, 4 pm J Z Young Lecture Theatre Anatomy Building, Gower Street University College London Dr. Knopfel is one of the main developers of a transformative new technology: voltage sensitive fluorescent proteins. His work received very considerable attention at the last SfN meeting. All the best, Kenneth. _______________________________________________ neuroscience-technology-network mailing list neuroscience-technology-network(a)imperial.ac.uk https://mailman.ic.ac.uk/mailman/listinfo/neuroscience-technology-network

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[FILM-Users 00015] Leica Matrix demonstration
by Martin Spitaler 14 Jan '11

14 Jan '11

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[FILM-Users 00014] Fwd: microscopist / imaging specialist positions open immediately: MPI-CBG Light Microscopy Facility, Dresden, Germany.
by Martin Spitaler 04 Jan '11

04 Jan '11

Dear microscopists, first of all, Happy New Year to everyone! If someone is just finishing his / her PhD or postdoc and is looking for a new job in microscopy, have a look at the advert below, a good opportunity to work in one of the best microscopy facilities around. Good imaging, Martin ############################### Martin Spitaler, PhD FILM - Facility for Imaging by Light Microscopy - Facility Manager - Sir Alexander Fleming Building, desk 401 Imperial College London / South Kensington Exhibition Road London SW7 2AZ UK Tel. +44-(0)20-759-42023 E-mail m.spitaler(a)imperial.ac.uk Website: http://imperial.ac.uk/imagingfacility ============================================================================================== ============================================================================================== Subject: microscopist / imaging specialist positions open immediately: MPI-CBG Light Microscopy Facility, Dresden, Germany. From: Daniel James White <white(a)MPI-CBG.DE> Reply-To: Confocal Microscopy List <CONFOCALMICROSCOPY(a)LISTS.UMN.EDU> Date: Tue, 21 Dec 2010 14:07:06 +0100 Content-Type: text/plain ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear friends of microscopy and imaging, We are very actively searching for 2 microscopists / imaging specialists to join our light microscopy facility team with immediate effect. see http://www.mpi-cbg.de/jobs/jobs-at-the-mpi-cbg/microscopistsimaging-special… In case of any questions, please feel free to contact us: peychl(a)mpi-cbg.de and pass on the advert to anyone who you think might be interested. cheers Dan White Full Text of advert follows: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) in Dresden is seeking motivated Microscopists/Imaging Specialists, to work in its Light Microscopy Facility (LMF). The LMF is a large core facility enabling different aspects of MPI-CBG researcher’s imaging projects including: design of imaging experiments, choice of and training on suitable imaging systems, and image processing, visualisation and analysis. The LMF is part of a network of core imaging facilities on the Dresden Biopolis campus (see https://ifn.mpi-cbg.de.) Light microscopy is one of the key methods of the MPI-CBG. The LMF provides more than 20 advanced imaging systems including laser scanning confocal microscopy, two-photon microscopy, wide-field microscopy, TIRF, SPIM and PALM/STORM. A super resolution structured illumination (SIM) system will be installed in 2011. The LMF team supports approximately 270 users from over 35 countries. More than 40 000 hours of instrument time is booked annually. The working language of the MPI-CBG is English. Requirements: We are seeking 1-2 candidates who are proactive and service-oriented team players with excellent interpersonal and organizational skills and who enjoy helping people. They should have a first degree (B.Sc.) and preferably also a doctorate degree (Ph.D.) in biology or physics/optics, be fluent in English and be ready to help scientists with both trivial and advanced aspects of light microscopy imaging projects. Experience in basic as well as advanced light microscopy is required and some experience with image analysis would be a strong plus. The successful candidate(s) should be ready to serve in a dynamic international multi-user environment whereby four basic areas of service must be covered: a) scientific support of imaging projects, b) implementation of new imaging technologies, c) maintenance and service of current imaging systems and d) training and teaching light microscopy. Ideally, the candidate(s) should have experience working in an imaging facility. The positions are available immediately. The initial contract is for 2 years. Compensation will depend on the qualifications and experience of the candidate. The Max Planck Society is committed to employ more disabled persons. The application of disabled persons is strongly encouraged. The Max Planck Society is committed to increase the number of female scientists. Women are particularly encouraged to apply. For informal inquiries, please contact the leader of the LMF, Dr. Jan Peychl, email: peychl(a)mpi-cbg.de If you wish to apply for this position, please send your CV, letter of application/motivation and the contact information of three referees to: Max Planck Institute of Molecular Cell Biology and Genetics Code: 2010-LMF-4100 Pfotenhauerstr. 108 01307 Dresden Germany or by email: 2010-LMF-4100(a)mpi-cbg.de Deadline for the applications is January 7, 2011 Dr. Daniel James White BSc. (Hons.) PhD Senior Microscopist / Image Visualisation, Processing and Analysis Light Microscopy and Image Processing Facilities Max Planck Institute of Molecular Cell Biology and Genetics Pfotenhauerstrasse 108 01307 DRESDEN Germany +49 (0)15114966933 (German Mobile) +49 (0)351 210 2627 (Work phone at MPI-CBG) +49 (0)351 210 1078 (Fax MPI-CBG LMF)

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